FastQCFastQC Report
Thu 26 May 2016
SRR936005_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936005_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences992800
Sequences flagged as poor quality0
Sequence length125
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179991.8129532634971797No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131911.3286663980660758No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102311.0305197421434327No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32550.3278605962933119No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28900.2910958904109589No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25750.2593674456083803No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21520.21676067687348913No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18590.18724818694601128No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16950.17072925060435132No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16260.16377921031426268No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC11010.11089846897663176No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG10620.10697018533440775No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA11350.055.340977100-101
TACGCAG7800.051.90444100-101
TCGAGTA2700.051.819633100-101
AGTATCA27600.050.80098100-101
ACGTATC3200.047.443905100-101
CGGTATC9850.047.146408100-101
CGAGTAC8000.046.88574100-101
CCGTATC4700.046.869907100-101
AGGTATC18500.046.66449100-101
CGTACAT10200.046.404186100-101
AAGTATC19550.045.37637100-101
TCGGTAT2900.044.13983100-101
CGAGCGT1350.044.0940496-97
GAGCGTA1100.043.29778398-99
CCTGTCG3650.043.21819796-97
CGACGCA900.042.999268100-101
GGTATCA94950.042.7966351
CCGGTAT3900.042.744835100-101
CCCGGTA2750.042.21533698-99
GCGTACA2050.042.111855100-101
Produced by FastQC (version 0.11.5)