FastQCFastQC Report
Thu 26 May 2016
SRR936004_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936004_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences997021
Sequences flagged as poor quality0
Sequence length77
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238892.39603779659606No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA134631.3503226110583428No Hit
CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG11920.11955615779406853No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTATA852.242814E-937.59038510
GCGGTAT1001.0966687E-831.9518289
CGCGTCA703.170259E-530.4257322
TCGCGAT703.170259E-530.42573226
TTCGCGA807.879828E-526.62118125
GTACTCG700.001128054125.3611464
ATTTGCG4500.025.2420883
ATGCACG851.1874015E-425.06277560
GCGTCAA851.1894267E-425.0564863
TTGCGCG4700.024.167965
GACGTAT750.001687103523.6680239
GATGGCG1802.401066E-1023.664465
GATCGTT1201.9184135E-623.6644572
TGCGCGC4900.023.181516
TCGCGCT952.5256E-422.42233737
AACTCGA800.002456670222.18654313
TCCGCAC1003.5688505E-421.30442667
CGCGCTT1003.572482E-421.30121838
ATACTCG1003.576117E-421.29801223
CGCTCCG1003.5773293E-421.29694425
Produced by FastQC (version 0.11.5)