FastQCFastQC Report
Thu 26 May 2016
SRR936004_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936004_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences997021
Sequences flagged as poor quality0
Sequence length125
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177061.7758903774343768No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133331.3372837683459025No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102211.0251539335680995No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32030.3212570246765113No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29560.29648322352287465No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26550.2662932877040704No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20880.20942387371981133No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18260.1831455907147392No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16950.17000644921220315No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16320.16368762543617438No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC11760.11795137715253741No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG10020.10049938767588648No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA11700.053.93688100-101
TCGAGTA3000.049.61175100-101
AGTATCA27200.048.809208100-101
CGGTATC11600.048.49976100-101
ACGTATC3300.047.807686100-101
TACGCAG7050.047.71173100-101
AGGTATC17600.047.694935100-101
GCGTATC2450.047.384285100-101
TCGTATC2900.047.216698100-101
CCTGTCG3700.045.8481596-97
GGTATCA99850.045.8366971
CGTACAT10100.045.68211100-101
CCGGTAT3650.045.669994100-101
TTCGGTA1050.045.35703798-99
CGAGTAC7250.045.163803100-101
TGTATCA20400.044.212826100-101
CCGTATC5000.044.055233100-101
TCGGTAT3200.043.720356100-101
CACGCAG8300.043.39534100-101
CCCGGTA2700.042.99469498-99
Produced by FastQC (version 0.11.5)