FastQCFastQC Report
Thu 26 May 2016
SRR936003_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936003_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3069614
Sequences flagged as poor quality0
Sequence length77
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42220.13754172348705734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGTCG4800.017.75160654
CGTTTAG2852.1645974E-1017.44813767
CGCTATC2803.252353E-916.47987439
CCGCTAT2803.252353E-916.47933638
GTACCCG3950.016.17841324
TACCCGC3751.8189894E-1216.09321425
AGGTCGG6250.015.33638855
CTACCGC3101.2494638E-814.88528935
TAAGGCG4301.8189894E-1214.86059917
TTGCGTA1951.2565129E-414.56162436
GGTCGGC5950.014.31992356
TGCGATA2504.545358E-614.20151553
GTCGATA2252.6541928E-514.20128422
ACCCTCG3808.4764906E-1014.01488362
TAAGTCG3051.6366175E-713.974853567
ACCGCTA3051.6490412E-713.966237
ACCGGTA2809.582109E-713.94609841
CTCGAAC9950.013.91605923
CGATTGC2303.2492877E-513.89958468
GTCGGCC6900.013.89097657
Produced by FastQC (version 0.11.5)