FastQCFastQC Report
Thu 26 May 2016
SRR936003_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936003_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3069614
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70700.23032211867681082No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50880.1657537397210203No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37150.12102498880966793No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA12100.044.032207100-101
GGTATCA74350.037.6994251
CGGTATC9750.036.633587100-101
TACGCAG9700.031.912764100-101
GCGTATC4000.031.25303100-101
GTATCAA146500.030.425591
ACCGTAC3400.028.8895998-99
CGTACAT18950.027.330202100-101
CCGTACA8050.026.621923100-101
CCTGTCG8350.026.01724896-97
CCGTATC5450.025.668718100-101
TATCAAC187850.023.5339372
TCGTACA9000.023.481112100-101
ATCAACG187450.023.3918023
TCAACGC188400.023.2730884
AGTATCA33450.023.046577100-101
TCGTATC6550.022.721214100-101
CAACGCA194400.022.6156235
TGTATCA31450.022.240782100-101
CCGTATA3100.022.083551100-101
Produced by FastQC (version 0.11.5)