FastQCFastQC Report
Thu 26 May 2016
SRR936002_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936002_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3087370
Sequences flagged as poor quality0
Sequence length77
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43530.14099379083167873No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCG800.002455271522.1905252
GTACCCG4000.022.18656324
GGTAACG2400.022.182251
TACCCGC4050.021.91123625
GGCACGC6450.019.81375942
TCGAACT10350.018.17797724
TTTACGC1200.001218280817.75385363
GGGCACG7150.017.37773341
CGATTCG1452.190035E-417.13831915
ACCCGCA5600.017.1131326
CTCGAAC11050.017.02670723
CGAACTC11000.016.78002225
ACGAGGT4900.016.6601334
GTAACGT3206.366463E-1116.6366882
CGCTGTA7200.016.2725246
GCACGCT8050.015.87562243
TACGTAG2502.6645466E-715.6163042
TAACGTT3502.4738256E-1015.2106873
GTAGACC22250.014.6768438
TCGCGTT1707.35148E-414.6165569
Produced by FastQC (version 0.11.5)