##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936002_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3087370 Sequences flagged as poor quality 0 Sequence length 77 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.08892099100529 40.0 37.0 41.0 31.0 41.0 2 36.95943375753473 40.0 37.0 41.0 31.0 41.0 3 36.82884493922011 40.0 36.0 41.0 30.0 41.0 4 36.73233075400746 39.0 36.0 41.0 30.0 41.0 5 36.65757878064502 39.0 36.0 41.0 30.0 41.0 6 36.57037348941008 39.0 36.0 41.0 30.0 41.0 7 36.43401600715172 39.0 36.0 41.0 30.0 41.0 8 36.30879356863609 39.0 36.0 40.0 30.0 41.0 9 36.482494485597776 39.0 36.0 41.0 30.0 41.0 10-11 36.70935100101381 39.5 36.0 41.0 30.0 41.0 12-13 36.650531844255795 40.0 36.0 41.0 30.0 41.0 14-15 36.50683461975727 40.0 36.0 41.0 30.0 41.0 16-17 36.37022190408017 40.0 36.0 41.0 30.0 41.0 18-19 36.22754010695188 40.0 35.5 41.0 29.5 41.0 20-21 36.03616881034667 39.5 35.0 41.0 29.0 41.0 22-23 35.78480211312541 39.0 35.0 41.0 27.5 41.0 24-25 35.56671487382465 39.0 35.0 41.0 27.0 41.0 26-27 34.62884995967442 38.0 34.0 40.0 25.5 40.5 28-29 34.66014407084347 38.0 34.0 40.0 25.5 40.5 30-31 35.08104438405504 39.0 35.0 40.0 26.0 41.0 32-33 34.96515853299086 39.0 34.5 40.5 26.0 41.0 34-35 34.72246685690409 38.0 34.0 40.0 25.5 41.0 36-37 34.42124963966094 38.0 34.0 40.0 24.5 41.0 38-39 34.1214135332014 38.0 34.0 40.0 23.0 41.0 40-41 33.773579454357595 37.0 33.0 40.0 22.5 41.0 42-43 33.40477671934365 36.5 33.0 39.5 22.0 41.0 44-45 33.04137664095978 36.0 33.0 39.0 21.5 41.0 46-47 32.62891344412883 35.5 32.0 39.0 20.0 40.5 48-49 32.20848683507322 35.0 32.0 38.5 18.5 40.0 50-51 31.760043175907 35.0 31.5 37.0 17.5 39.5 52-53 31.278862105934824 35.0 31.0 37.0 12.0 39.0 54-55 30.823632897903394 35.0 31.0 36.0 7.5 39.0 56-57 30.392966667422435 34.5 30.5 36.0 7.0 37.5 58-59 29.9653761939774 34.0 30.0 35.5 2.0 37.0 60-61 29.602536463073747 34.0 30.0 35.0 2.0 37.0 62-63 29.24007035114029 34.0 29.5 35.0 2.0 36.0 64-65 28.868131289738514 34.0 29.0 35.0 2.0 36.0 66-67 28.519397739823866 34.0 29.0 35.0 2.0 35.5 68-69 28.160968720950194 34.0 29.0 35.0 2.0 35.0 70-71 27.72285067873303 34.0 27.5 35.0 2.0 35.0 72-73 27.259965115940105 34.0 27.0 35.0 2.0 35.0 74-75 26.708997463860825 33.0 25.5 35.0 2.0 35.0 76-77 24.939682318607744 31.0 21.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.9568934205104824 1101 2 0.9596749898033252 1101 3 0.9751420364454049 1101 4 0.8594953989934027 1101 5 0.9217835653411726 1101 6 0.9789409217236766 1101 7 0.9366987793459316 1101 8 0.8578175950143745 1101 9 0.9692490415458295 1101 10-11 0.9463394146425088 1101 12-13 1.0685595649777326 1101 14-15 1.0782289767079618 1101 16-17 1.0902481046349592 1101 18-19 1.112855296869263 1101 20-21 1.120282397351076 1101 22-23 1.0203522536408087 1101 24-25 1.1019368278606123 1101 26-27 1.0336591712895498 1101 28-29 1.0895580804106046 1101 30-31 1.1508844410082943 1101 32-33 1.1181595857467101 1101 34-35 1.0811343451214768 1101 36-37 1.0460223025340412 1101 38-39 1.05838799887416 1101 40-41 0.9700890441050873 1101 42-43 0.997253849180062 1101 44-45 0.9277145575075352 1101 46-47 0.9653776313558851 1101 48-49 0.8452990759557721 1101 50-51 0.851577556730863 1101 52-53 0.8824838938482635 1101 54-55 1.0136996184280136 1101 56-57 0.9814679538495596 1101 58-59 1.0004812897912458 1101 60-61 0.8072052907685006 1101 62-63 0.7517549037236684 1101 64-65 0.9566133733535978 1101 66-67 0.941255680811377 1101 68-69 0.939815301127858 1101 70-71 0.9062760374559993 1101 72-73 0.7330914473987491 1101 74-75 0.8109896347259777 1101 76-77 1.0992780831859932 1102 1 0.5888712839419696 1102 2 0.7220538810792831 1102 3 0.613977075832068 1102 4 0.36806199826043695 1102 5 0.496733436860616 1102 6 0.4886405720880589 1102 7 0.4481544008969607 1102 8 0.46461316348819537 1102 9 0.28799941013760133 1102 10-11 0.4692170632376502 1102 12-13 0.5789227331819546 1102 14-15 0.6186121015236665 1102 16-17 0.6128623680832916 1102 18-19 0.653312192694699 1102 20-21 0.6670419491434814 1102 22-23 0.6792180967681531 1102 24-25 0.8146668817803189 1102 26-27 0.6800223816185422 1102 28-29 0.7248575875507584 1102 30-31 0.8168793755042643 1102 32-33 0.7436340686651164 1102 34-35 0.7178052242655042 1102 36-37 0.7194087657380521 1102 38-39 0.8501055312049246 1102 40-41 0.8047454639205753 1102 42-43 0.8710676155560648 1102 44-45 0.8082121563954416 1102 46-47 0.7648671317560698 1102 48-49 0.6569491033368777 1102 50-51 0.702963725061462 1102 52-53 0.6692175915608836 1102 54-55 0.7550528241232826 1102 56-57 0.6107523066024143 1102 58-59 0.6447767529531347 1102 60-61 0.5617155902756394 1102 62-63 0.5255518728462114 1102 64-65 0.5698555592080972 1102 66-67 0.515989083650588 1102 68-69 0.4858056018987398 1102 70-71 0.45566696588600664 1102 72-73 0.5168766215846858 1102 74-75 0.46627187070895815 1102 76-77 0.4099021617065688 1103 1 0.5011117996752859 1103 2 0.5126257095261622 1103 3 0.4745632751379958 1103 4 0.6031144496029626 1103 5 0.38313006589984155 1103 6 0.4760932737428547 1103 7 0.5429476639783246 1103 8 0.3700631669068457 1103 9 0.5096327018356064 1103 10-11 0.5256560377411574 1103 12-13 0.5599820806190507 1103 14-15 0.5143533326731173 1103 16-17 0.4774544391379578 1103 18-19 0.43116354915036936 1103 20-21 0.5504547592613704 1103 22-23 0.5066979037725616 1103 24-25 0.7369825808285952 1103 26-27 0.7160643356051111 1103 28-29 0.5449381427403566 1103 30-31 0.6781047486796652 1103 32-33 0.6806583231898315 1103 34-35 0.6633623066350793 1103 36-37 0.6936754120873445 1103 38-39 0.5844175518636092 1103 40-41 0.6465122270044361 1103 42-43 0.6686479998129116 1103 44-45 0.5767019738059034 1103 46-47 0.5958362600490332 1103 48-49 0.4901954416236407 1103 50-51 0.4737972494781637 1103 52-53 0.6084717890023974 1103 54-55 0.5588843965712549 1103 56-57 0.649794130513321 1103 58-59 0.6447263069437028 1103 60-61 0.5094084983171498 1103 62-63 0.35955172234200106 1103 64-65 0.2843754945538244 1103 66-67 0.4063804842319243 1103 68-69 0.5234373347733623 1103 70-71 0.5447007851408721 1103 72-73 0.6410540076809355 1103 74-75 0.6480540656857059 1103 76-77 0.6642957589823197 1104 1 0.5045142549605899 1104 2 0.5927686775598531 1104 3 0.721095231982531 1104 4 0.7066094040701074 1104 5 0.5853124515673329 1104 6 0.5150430023307848 1104 7 0.5120834998382549 1104 8 0.4744182014858538 1104 9 0.46073931119966005 1104 10-11 0.6356705617354947 1104 12-13 0.6720981211131374 1104 14-15 0.7270121973132788 1104 16-17 0.6591612383687533 1104 18-19 0.6577980394447209 1104 20-21 0.7597950129038651 1104 22-23 0.8071971829821507 1104 24-25 0.7888071407137076 1104 26-27 0.674985367809434 1104 28-29 0.6791059070459511 1104 30-31 0.9445111825116541 1104 32-33 0.9146102560711213 1104 34-35 0.9697712557956919 1104 36-37 0.9839152608430695 1104 38-39 0.930091321854384 1104 40-41 0.9897166474916546 1104 42-43 1.0328662329703562 1104 44-45 0.921873354234485 1104 46-47 0.8323819565381214 1104 48-49 0.9169919407189369 1104 50-51 0.8912207686122393 1104 52-53 0.9627071426224383 1104 54-55 0.765183266296166 1104 56-57 0.6672337423413168 1104 58-59 0.7894550706732062 1104 60-61 0.7812898288989487 1104 62-63 0.6620456842009652 1104 64-65 0.64202547440415 1104 66-67 0.7634489633776873 1104 68-69 0.6541965583211677 1104 70-71 0.5910098784595661 1104 72-73 0.5457000630776676 1104 74-75 0.5786844124494479 1104 76-77 0.6476952661942796 1105 1 -0.09893583223711033 1105 2 0.09636761676783578 1105 3 0.020455658606486793 1105 4 0.04605666995984592 1105 5 -0.005753626283130586 1105 6 0.0365721611335843 1105 7 0.15645223424185417 1105 8 0.1580930539831087 1105 9 0.17364115085889154 1105 10-11 0.31425404101631216 1105 12-13 0.3454530344860416 1105 14-15 0.2806311592383892 1105 16-17 0.22123475140391236 1105 18-19 0.15596571363308698 1105 20-21 0.20894225526896548 1105 22-23 0.24833667746209898 1105 24-25 0.3263780596534218 1105 26-27 0.1273760019569039 1105 28-29 0.23310142788997013 1105 30-31 0.4159679958591411 1105 32-33 0.4865178689471392 1105 34-35 0.4059370002226004 1105 36-37 0.34842697227863795 1105 38-39 0.3798910534840587 1105 40-41 0.4028462764575167 1105 42-43 0.5042334384788205 1105 44-45 0.2588913207092034 1105 46-47 0.29001815118365215 1105 48-49 0.11789168582068754 1105 50-51 0.1925730642002783 1105 52-53 0.10202158291539831 1105 54-55 0.01977448357436984 1105 56-57 0.09899737292960253 1105 58-59 0.1512955415284125 1105 60-61 0.3597363420828401 1105 62-63 0.22937557247337992 1105 64-65 0.18318070690456167 1105 66-67 0.16773347465241173 1105 68-69 0.1004795427607128 1105 70-71 0.006259093309498809 1105 72-73 -0.335099881550871 1105 74-75 -0.2830259725429052 1105 76-77 -0.2665587866549828 1106 1 0.10866383672210844 1106 2 0.21070035080320082 1106 3 0.22318422212359224 1106 4 0.1638226492743442 1106 5 0.08042471800838058 1106 6 0.1250165879313485 1106 7 -0.17572510336142244 1106 8 0.055029520540507804 1106 9 0.06461937967490883 1106 10-11 -0.058689206831246565 1106 12-13 0.29184140603754827 1106 14-15 0.177862621843893 1106 16-17 0.13841437825097103 1106 18-19 0.1853111512136607 1106 20-21 0.18570581050798296 1106 22-23 0.2142708895176284 1106 24-25 -0.01579709003664931 1106 26-27 -0.010897631124677787 1106 28-29 0.1569003193748486 1106 30-31 0.3942989696376813 1106 32-33 0.16414012842040648 1106 34-35 0.1569412068889946 1106 36-37 0.25740905910114975 1106 38-39 0.20361752462821414 1106 40-41 0.22807419786966676 1106 42-43 0.2394631631615809 1106 44-45 0.2426158727763692 1106 46-47 0.25387074373671936 1106 48-49 0.11880097262302058 1106 50-51 0.18902235817433777 1106 52-53 0.19515637449908851 1106 54-55 0.17609643603668346 1106 56-57 0.2613838568372735 1106 58-59 0.1202359628733447 1106 60-61 -0.029414420853662193 1106 62-63 0.0045227189423791 1106 64-65 0.1646661634856521 1106 66-67 0.058286011659784975 1106 68-69 -0.020846448448757826 1106 70-71 -0.10833399826148948 1106 72-73 -0.22360998710423985 1106 74-75 -0.140575762044147 1106 76-77 -0.2086026615526677 1107 1 -0.20529585961890007 1107 2 -0.2328061518264164 1107 3 -0.11884721358201489 1107 4 -0.17627950974407014 1107 5 -0.2592918910989894 1107 6 -0.41820898350615465 1107 7 -0.18029102425150967 1107 8 -0.14315056578113428 1107 9 -0.28933203350244696 1107 10-11 -0.16974824839916636 1107 12-13 -0.2805229118526782 1107 14-15 -0.3186538883867698 1107 16-17 -0.3229381882571474 1107 18-19 -0.3479407215082446 1107 20-21 -0.37951071542159553 1107 22-23 -0.3210296816093603 1107 24-25 -0.339762249437662 1107 26-27 -0.5398221718702914 1107 28-29 -0.322340103522599 1107 30-31 -0.14806214331164114 1107 32-33 -0.12536331342956686 1107 34-35 -0.36358232651966915 1107 36-37 -0.2089498531492353 1107 38-39 -0.15103830360975934 1107 40-41 -0.12097970316852269 1107 42-43 -0.18651593712150571 1107 44-45 -0.20613744797203992 1107 46-47 -0.2565638943034898 1107 48-49 -0.2722329201360729 1107 50-51 -0.2549244674986646 1107 52-53 -0.27879462329838134 1107 54-55 -0.2785464217606588 1107 56-57 -0.12514861090409823 1107 58-59 -0.22485839046466793 1107 60-61 -0.21786255344618155 1107 62-63 -0.21028259107864145 1107 64-65 -0.3036630034923782 1107 66-67 -0.13138326653824706 1107 68-69 -0.3914216600023437 1107 70-71 -0.3389232349948408 1107 72-73 -0.21760235185176313 1107 74-75 -0.3978565671969747 1107 76-77 -0.33144525680548753 1108 1 0.12090694179785544 1108 2 0.17452244259845173 1108 3 0.20868063269548998 1108 4 0.2780683330007676 1108 5 0.15780252365223646 1108 6 0.13375884761114065 1108 7 0.2953910427870383 1108 8 0.21621509846342235 1108 9 0.23281619744280846 1108 10-11 0.2550527541866785 1108 12-13 0.2708541111542573 1108 14-15 0.2544046821719661 1108 16-17 0.2952023124738403 1108 18-19 0.2560149814404369 1108 20-21 0.2424784865630656 1108 22-23 0.23142708398836476 1108 24-25 0.2554900857476028 1108 26-27 0.17454548760418476 1108 28-29 0.3232241005398109 1108 30-31 0.3932015396332531 1108 32-33 0.30307021594255446 1108 34-35 0.4037156586616035 1108 36-37 0.35594644892641014 1108 38-39 0.4762514216536644 1108 40-41 0.41917299724893553 1108 42-43 0.44616267721637115 1108 44-45 0.38041947679217714 1108 46-47 0.40003891190733043 1108 48-49 0.4341714535336507 1108 50-51 0.3880781599961374 1108 52-53 0.5863542605419063 1108 54-55 0.568996694755068 1108 56-57 0.5777152366104268 1108 58-59 0.5885922295105388 1108 60-61 0.6613527013096068 1108 62-63 0.706362577665157 1108 64-65 0.6902445211866812 1108 66-67 0.7564363803954564 1108 68-69 0.625660101592139 1108 70-71 0.549989809722085 1108 72-73 0.5779834450976864 1108 74-75 0.574979178127986 1108 76-77 0.5380370876201184 1109 1 -0.15582130852954634 1109 2 -0.163681682249198 1109 3 -0.23025356766836325 1109 4 -0.21026685383417032 1109 5 -0.2902694725393644 1109 6 -0.2701129625060119 1109 7 -0.19793793168994966 1109 8 -0.23624821504435545 1109 9 -0.26439308099526926 1109 10-11 -0.2596691514368388 1109 12-13 -0.21685322258862527 1109 14-15 -0.08528003819291996 1109 16-17 -0.0753398515600665 1109 18-19 -0.044316871066932606 1109 20-21 0.10522251173644293 1109 22-23 0.032336814180418116 1109 24-25 -0.014030446638628291 1109 26-27 -0.10772371193991148 1109 28-29 0.09012744257110938 1109 30-31 0.12135942241425823 1109 32-33 0.11219487594188848 1109 34-35 0.09445075147166193 1109 36-37 0.19025071421879147 1109 38-39 0.20341967285020957 1109 40-41 0.2467890517991691 1109 42-43 0.26379125524812963 1109 44-45 0.28501316487847106 1109 46-47 0.2702851741221721 1109 48-49 0.39879706267065984 1109 50-51 0.32359032890905937 1109 52-53 0.24007179874939055 1109 54-55 0.27851098515366957 1109 56-57 0.31894326866579803 1109 58-59 0.2922555453886915 1109 60-61 0.25060838378996664 1109 62-63 0.12500360214125195 1109 64-65 0.15813551948988547 1109 66-67 0.18426201560658484 1109 68-69 0.05116484332990012 1109 70-71 0.05291090191189696 1109 72-73 0.002194060822358068 1109 74-75 -0.019376860106163463 1109 76-77 0.06126982486997079 1110 1 -0.25404671435439496 1110 2 -0.29026823027164284 1110 3 -0.593548206452077 1110 4 -0.3860109472937907 1110 5 -0.30057837904464435 1110 6 -0.355818140452854 1110 7 -0.41382017911930546 1110 8 -0.7140104170852766 1110 9 -0.6560841510938857 1110 10-11 -0.5621460185430891 1110 12-13 -0.36714606377199743 1110 14-15 -0.38619297350659565 1110 16-17 -0.5219847533549355 1110 18-19 -0.49712440305134464 1110 20-21 -0.6307670263075664 1110 22-23 -0.679086429064931 1110 24-25 -0.7612234020729574 1110 26-27 -0.7152448756619236 1110 28-29 -0.533704604626628 1110 30-31 -0.39139544884870503 1110 32-33 -0.4671689841026492 1110 34-35 -0.4997849369390863 1110 36-37 -0.4510847351617997 1110 38-39 -0.600684880420765 1110 40-41 -0.5404067501606491 1110 42-43 -0.585487365005875 1110 44-45 -0.6181461859577482 1110 46-47 -0.5966981522573249 1110 48-49 -0.5769952852449443 1110 50-51 -0.48985872413379994 1110 52-53 -0.4557109567652624 1110 54-55 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-0.4827510742107961 2314 12-13 -0.49042922637559627 2314 14-15 -0.5803754705322888 2314 16-17 -0.6466833377158068 2314 18-19 -0.5934705354394723 2314 20-21 -0.5327497036029882 2314 22-23 -0.4707719945887803 2314 24-25 -0.43182845774066436 2314 26-27 -0.42521329222992676 2314 28-29 -0.542876226054382 2314 30-31 -0.7145650009421658 2314 32-33 -0.7638344286834666 2314 34-35 -0.8529812106882417 2314 36-37 -1.3674355554325786 2314 38-39 -0.9221084317056167 2314 40-41 -0.7913138953053149 2314 42-43 -0.709481680133031 2314 44-45 -0.7440861172987425 2314 46-47 -0.5632082301181995 2314 48-49 -0.6033331069662893 2314 50-51 -0.44819869923802713 2314 52-53 -0.3345748371728128 2314 54-55 -0.47822567632317714 2314 56-57 -0.4661256659905071 2314 58-59 -0.5025636305758923 2314 60-61 -0.41539886100572687 2314 62-63 -0.44518668931477023 2314 64-65 -0.297711629744029 2314 66-67 -0.20452473074420396 2314 68-69 -0.28600347335531495 2314 70-71 -0.19247123424884194 2314 72-73 -0.0393351864807201 2314 74-75 -0.11038761820247345 2314 76-77 -0.15369082616312468 2315 1 -1.066209174533455 2315 2 -0.8845468017399298 2315 3 -0.7566564545529957 2315 4 -0.6973042499626061 2315 5 -0.8965360553362913 2315 6 -0.840834068829821 2315 7 -1.2331665319008849 2315 8 -1.1116893588257781 2315 9 -0.7251969062119983 2315 10-11 -0.8566820014786956 2315 12-13 -0.8138904295372029 2315 14-15 -0.7290988228069182 2315 16-17 -0.600709343470264 2315 18-19 -0.6484286952810052 2315 20-21 -0.5881524096356756 2315 22-23 -0.6641891342018624 2315 24-25 -0.7738628332844257 2315 26-27 -0.6476326218382766 2315 28-29 -0.6285535954587758 2315 30-31 -0.7008855276659105 2315 32-33 -0.7054373962500975 2315 34-35 -0.7652544117196172 2315 36-37 -0.772164213963805 2315 38-39 -0.6820993184427877 2315 40-41 -0.7728253523552198 2315 42-43 -0.8323598805404444 2315 44-45 -0.8253493983194247 2315 46-47 -0.5679142592841586 2315 48-49 -0.6094218562610436 2315 50-51 -0.6250012270479779 2315 52-53 -0.5404027116294898 2315 54-55 -0.7465780476170139 2315 56-57 -0.8057579453959391 2315 58-59 -0.6561340610699062 2315 60-61 -0.8064210667844307 2315 62-63 -0.7721728884777903 2315 64-65 -0.6893124442254184 2315 66-67 -0.9050606732067372 2315 68-69 -1.0044542714029525 2315 70-71 -0.9334889580950083 2315 72-73 -0.8481462264472555 2315 74-75 -0.7706502770254637 2315 76-77 -0.7269158929966473 2316 1 -1.7065228560765249 2316 2 -1.3089790300702049 2316 3 -1.5228344766530597 2316 4 -2.334123719015956 2316 5 -1.7681088547951447 2316 6 -1.7143513505929846 2316 7 -1.4082991676032393 2316 8 -1.3008043972661554 2316 9 -1.5502697233387863 2316 10-11 -1.4434002585118009 2316 12-13 -1.9702685536064806 2316 14-15 -3.0462031494314843 2316 16-17 -2.250905709933491 2316 18-19 -1.7489743820335875 2316 20-21 -1.632587767714213 2316 22-23 -2.043573179552922 2316 24-25 -1.927241951805975 2316 26-27 -1.901909523791339 2316 28-29 -2.363886938888065 2316 30-31 -2.4437464204518378 2316 32-33 -2.504965157621932 2316 34-35 -2.3703980520206756 2316 36-37 -2.437617345983657 2316 38-39 -2.5983328683345093 2316 40-41 -2.2818145446277747 2316 42-43 -2.4930951694616397 2316 44-45 -2.3591163532258115 2316 46-47 -2.3568569091713343 2316 48-49 -2.05823381391858 2316 50-51 -2.1568645882966457 2316 52-53 -2.229081352107233 2316 54-55 -2.3041808118914417 2316 56-57 -2.1409267246426786 2316 58-59 -2.2051613151196463 2316 60-61 -2.109990241792886 2316 62-63 -2.2312269473069613 2316 64-65 -2.1917975653012434 2316 66-67 -2.132586809128675 2316 68-69 -2.1901871625331424 2316 70-71 -2.010080207924638 2316 72-73 -2.057377469411634 2316 74-75 -2.2572365293538468 2316 76-77 -2.7531371258145825 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 63634.0 3 5423.0 4 7032.0 5 8714.0 6 10146.0 7 10712.0 8 12612.0 9 13258.0 10 15983.0 11 18046.0 12 14689.0 13 12432.0 14 12088.0 15 12374.0 16 12810.0 17 13333.0 18 14096.0 19 14818.0 20 16196.0 21 17616.0 22 19368.0 23 22539.0 24 25800.0 25 30108.0 26 35871.0 27 42655.0 28 50897.0 29 61460.0 30 74658.0 31 91698.0 32 114825.0 33 146706.0 34 196406.0 35 270727.0 36 391919.0 37 547141.0 38 510070.0 39 147106.0 40 1404.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.419318006980266 26.615316095909826 28.616304022155457 22.34906187495445 2 22.229989310355997 26.739107900618702 28.705808039908003 22.325094749117294 3 22.364413582386515 27.067170111545263 28.194252150471105 22.37416415559712 4 22.104853598966685 26.953301535169068 28.493070892109884 22.448773973754367 5 22.354159222601496 27.026985876819904 28.484429904198727 22.134424996379877 6 22.54523037371567 26.87815909649719 28.298798899485217 22.27781163030193 7 22.433678102346086 26.69514490892027 28.458264721308563 22.412912267425078 8 22.266724202835732 26.75885412313388 28.391475316789094 22.582946357241298 9 22.329150371516466 26.615358000886353 28.417640488167713 22.63785113942947 10-11 22.598869690425932 26.863521331114548 28.296891494784116 22.240717483675404 12-13 22.630360906978776 26.722534141939825 28.2678366720289 22.3792682790525 14-15 22.307553393167908 27.031742342586018 28.337391859247568 22.323312404998504 16-17 22.597538686289802 26.7766054536959 28.30671107623562 22.319144783778686 18-19 22.441886342484686 26.751670143060714 28.207981766180946 22.598461748273653 20-21 22.53566158267117 26.97274298798477 28.102611546560535 22.388983882783528 22-23 22.68799108495051 27.02899172798733 27.756206439114777 22.526810747947383 24-25 22.64767741797535 26.979318325690855 27.835217873880598 22.537786382453202 26-27 22.654917654277053 27.133749460835915 27.71385195343825 22.49748093144878 28-29 22.70653921884138 27.155767698658895 27.647407235126465 22.490285847373258 30-31 22.704575866718972 27.116973656122788 27.629213013636285 22.54923746352196 32-33 22.52889640700446 27.35019575246626 27.556071112274388 22.564836728254893 34-35 22.669278461455484 27.15126862576111 27.6716731645698 22.50777974821361 36-37 22.627520759193356 27.158667809499377 27.778333861342773 22.43547756996449 38-39 22.466116922271514 27.128944805323652 27.885336033407754 22.51960223899708 40-41 22.530515710280174 27.073182148675944 27.716805656539872 22.679496484504007 42-43 22.45635637372119 27.262366794189198 27.553856119016277 22.727420713073336 44-45 22.591940298555816 27.07803629390897 27.699904740353887 22.630118667181325 46-47 22.544344031318573 26.93231646348545 27.67893312893999 22.84440637625599 48-49 22.536468034791625 27.068708595863235 27.57224768784723 22.82257568149792 50-51 22.468667702450453 27.228089341778354 27.461181192019634 22.842061763751552 52-53 22.54367313613091 27.188079402609333 27.538695690560832 22.729551770698926 54-55 22.45089801550989 27.16623067075335 27.53990905561001 22.84296225812675 56-57 22.443047635831412 27.115341131071585 27.50448835360516 22.937122879491838 58-59 22.593908510219567 27.179768355903555 27.52473194739941 22.701591186477465 60-61 22.61197980278363 27.099692169334727 27.405676239373168 22.882651788508475 62-63 22.459514317603436 27.113217358342634 27.606351355948405 22.82091696810553 64-65 22.642020443849066 27.053508587923048 27.45916211157537 22.84530885665251 66-67 22.47641376486268 27.13782551142655 27.557894075272504 22.827866648438263 68-69 22.4897350603941 26.99624847358331 27.636849391717483 22.877167074305106 70-71 22.419139037574396 27.028943482437988 27.702334498889456 22.849582981098155 72-73 22.567317277046385 26.87458260184839 27.628050581890097 22.930049539215126 74-75 22.38473403540641 26.85203418534126 27.54937469337585 23.21385708587648 76-77 22.36597848420934 26.745196158417777 27.777453637389982 23.1113717199829 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3535.0 1 2311.0 2 1064.5 3 1135.5 4 1229.0 5 1286.0 6 1402.5 7 1542.0 8 1777.0 9 1932.0 10 1973.5 11 2103.0 12 2384.0 13 2577.0 14 2679.5 15 2998.5 16 3497.5 17 3780.0 18 4148.0 19 5169.0 20 6697.0 21 9151.0 22 10730.0 23 12681.0 24 17037.5 25 22625.5 26 25808.0 27 29912.0 28 38577.5 29 47136.0 30 51133.0 31 56950.5 32 69237.5 33 80770.5 34 91429.5 35 97025.0 36 102457.5 37 112660.0 38 119705.0 39 121980.0 40 124232.5 41 130629.5 42 136001.5 43 137229.0 44 138349.0 45 140811.0 46 143815.0 47 145667.5 48 145858.0 49 145518.0 50 143780.5 51 137780.5 52 133178.0 53 129845.5 54 122348.5 55 112385.5 56 100756.0 57 94925.0 58 88332.0 59 73783.0 60 59023.0 61 52219.0 62 46004.5 63 34008.0 64 23758.0 65 19290.0 66 16129.0 67 11413.5 68 8474.0 69 5986.5 70 4884.0 71 4286.5 72 3177.0 73 2159.0 74 1653.0 75 1401.5 76 946.0 77 605.0 78 468.0 79 370.0 80 255.0 81 203.5 82 151.5 83 134.0 84 120.0 85 90.0 86 73.5 87 73.0 88 65.0 89 50.0 90 39.5 91 36.0 92 31.5 93 30.5 94 28.0 95 25.5 96 29.0 97 25.0 98 26.5 99 63.5 100 95.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0030770526370341097 2 0.008745307494728523 3 0.01204909032607041 4 0.019725526904776556 5 0.01486702274103849 6 0.01460790251897246 7 0.018429925794446408 8 0.019952257099084332 9 0.018203195600138628 10-11 0.022462484250349003 12-13 0.021393613334326628 14-15 0.008016531870167813 16-17 0.006397030482255124 18-19 0.011628019965213109 20-21 0.010672514146344623 22-23 0.014737462630005473 24-25 0.02189565876457956 26-27 0.01345805653355445 28-29 0.008113701953442574 30-31 0.007741216634222656 32-33 0.009976128549542166 34-35 0.011287924673751445 36-37 0.00984656843850915 38-39 0.017539200031094427 40-41 0.012777865950631118 42-43 0.015045167893708886 44-45 0.017603980086610932 46-47 0.01656749919834681 48-49 0.01360381165846659 50-51 0.026511237720130726 52-53 0.030689551300945465 54-55 0.024567836054635497 56-57 0.015935893657060864 58-59 0.016923789503687607 60-61 0.0196931368770183 62-63 0.025426171790229223 64-65 0.020162792279512985 66-67 0.01982269698805132 68-69 0.028924294788120634 70-71 0.05566226270255914 72-73 0.06596229152968384 74-75 0.07655383060663283 76-77 0.07407599348312642 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 3087370.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.99766696529949 #Duplication Level Percentage of deduplicated Percentage of total 1 77.75896279235455 45.875973903904494 2 12.64909515896973 14.925342072025567 3 4.154705206205014 7.353537422840381 4 1.86765843905797 4.407499623698929 5 0.9686256892650646 2.8573327914646978 6 0.5852601122203134 2.0717388713308713 7 0.3873229925329173 1.5995807045022168 8 0.2720836242468452 1.2841839240021646 9 0.20121929082480575 1.0684321836368071 >10 1.0395153982767353 11.159988644734838 >50 0.07752050749919898 3.1120026358129826 >100 0.037094793173665166 3.7815463455968596 >500 8.258782704554452E-4 0.3246377080571371 >1k 1.101171026104975E-4 0.17820316839215083 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4353 0.14099379083167873 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.239002775825379E-5 0.0 4 0.0 0.0 0.0 3.239002775825379E-4 0.0 5 0.0 0.0 0.0 7.773606661980909E-4 0.0 6 0.0 0.0 0.0 0.0014575512491214204 0.0 7 0.0 0.0 0.0 0.0035629030534079166 0.0 8 0.0 0.0 0.0 0.005538694746661398 6.478005551650758E-5 9 0.0 0.0 0.0 0.008000336856288686 9.717008327476136E-5 10 0.0 0.0 0.0 0.010818269271256765 9.717008327476136E-5 11 0.0 0.0 0.0 0.014543122463455951 9.717008327476136E-5 12 0.0 0.0 0.0 0.01881860612754545 9.717008327476136E-5 13 0.0 0.0 0.0 0.025329001706954464 9.717008327476136E-5 14 0.0 0.0 0.0 0.03271392803583632 9.717008327476136E-5 15 0.0 0.0 0.0 0.040876215030916284 9.717008327476136E-5 16 0.0 0.0 0.0 0.05052844330287591 9.717008327476136E-5 17 0.0 0.0 0.0 0.06063413196345109 9.717008327476136E-5 18 0.0 0.0 0.0 0.0709989408460923 9.717008327476136E-5 19 0.0 0.0 0.0 0.08223828047820637 9.717008327476136E-5 20 0.0 0.0 0.0 0.0939958605544525 9.717008327476136E-5 21 0.0 0.0 0.0 0.10620690101931417 1.2956011103301517E-4 22 0.0 0.0 0.0 0.12029656309415457 1.2956011103301517E-4 23 0.0 0.0 0.0 0.13448339525226974 1.2956011103301517E-4 24 0.0 0.0 0.0 0.1513262096865617 1.2956011103301517E-4 25 0.0 0.0 0.0 0.16816902412085366 1.2956011103301517E-4 26 0.0 0.0 0.0 0.18705241030391562 1.2956011103301517E-4 27 0.0 0.0 0.0 0.20719900756954948 1.2956011103301517E-4 28 0.0 0.0 0.0 0.22763711508500764 1.2956011103301517E-4 29 0.0 0.0 0.0 0.2474274220453007 1.2956011103301517E-4 30 0.0 0.0 0.0 0.2675416292831763 1.6195013879126896E-4 31 0.0 0.0 0.0 0.2922876104904822 1.6195013879126896E-4 32 0.0 0.0 0.0 0.3192037235575911 1.6195013879126896E-4 33 0.0 0.0 0.0 0.3460226665414252 1.9434016654952272E-4 34 0.0 0.0 0.0 0.37591866216229347 1.9434016654952272E-4 35 0.0 0.0 0.0 0.4073045990600414 1.9434016654952272E-4 36 0.0 0.0 0.0 0.4406015475955263 1.9434016654952272E-4 37 0.0 0.0 0.0 0.47580950776874814 1.9434016654952272E-4 38 0.0 0.0 0.0 0.5116652684971351 1.9434016654952272E-4 39 0.0 0.0 0.0 0.5492377006967095 1.9434016654952272E-4 40 0.0 0.0 0.0 0.5874579334514489 1.9434016654952272E-4 41 0.0 0.0 0.0 0.6299212598425197 1.9434016654952272E-4 42 0.0 0.0 0.0 0.6764657297311304 1.9434016654952272E-4 43 0.0 0.0 0.0 0.726702662784182 1.9434016654952272E-4 44 0.0 0.0 0.0 0.7801138185575425 1.9434016654952272E-4 45 0.0 0.0 0.0 0.8388045488554984 1.9434016654952272E-4 46 0.0 0.0 0.0 0.9005399417627301 1.9434016654952272E-4 47 0.0 0.0 0.0 0.9650932670849299 2.267301943077765E-4 48 0.0 0.0 0.0 1.0315576040448666 2.5912022206603033E-4 49 0.0 0.0 0.0 1.0977304307549791 2.915102498242841E-4 50 0.0 0.0 0.0 1.1674985505462578 2.915102498242841E-4 51 0.0 0.0 0.0 1.2402141628635375 2.915102498242841E-4 52 0.0 0.0 0.0 1.320930112037106 2.915102498242841E-4 53 0.0 0.0 0.0 1.4057272047082143 2.915102498242841E-4 54 0.0 0.0 0.0 1.4998202353459418 2.915102498242841E-4 55 0.0 0.0 0.0 1.5990956704249895 2.915102498242841E-4 56 0.0 0.0 0.0 1.7064038323880844 2.915102498242841E-4 57 0.0 0.0 0.0 1.8114123023803432 2.915102498242841E-4 58 0.0 0.0 0.0 1.9217651269527138 3.239002775825379E-4 59 0.0 0.0 0.0 2.03386701302403 3.239002775825379E-4 60 0.0 0.0 0.0 2.1536453356740526 3.5629030534079165E-4 61 0.0 0.0 0.0 2.2739742887959653 3.5629030534079165E-4 62 0.0 0.0 0.0 2.396538153833198 3.5629030534079165E-4 63 0.0 0.0 0.0 2.5349731324719746 3.8868033309904544E-4 64 0.0 0.0 0.0 2.6753191227484883 3.8868033309904544E-4 65 0.0 0.0 0.0 2.8266777224628084 3.8868033309904544E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAACGCG 80 0.0024552715 22.19052 52 GTACCCG 400 0.0 22.186563 24 GGTAACG 240 0.0 22.18225 1 TACCCGC 405 0.0 21.911236 25 GGCACGC 645 0.0 19.813759 42 TCGAACT 1035 0.0 18.177977 24 TTTACGC 120 0.0012182808 17.753853 63 GGGCACG 715 0.0 17.377733 41 CGATTCG 145 2.190035E-4 17.138319 15 ACCCGCA 560 0.0 17.11313 26 CTCGAAC 1105 0.0 17.026707 23 CGAACTC 1100 0.0 16.780022 25 ACGAGGT 490 0.0 16.660133 4 GTAACGT 320 6.366463E-11 16.636688 2 CGCTGTA 720 0.0 16.27252 46 GCACGCT 805 0.0 15.875622 43 TACGTAG 250 2.6645466E-7 15.616304 2 TAACGTT 350 2.4738256E-10 15.210687 3 GTAGACC 2225 0.0 14.676843 8 TCGCGTT 170 7.35148E-4 14.616556 9 >>END_MODULE