FastQCFastQC Report
Thu 26 May 2016
SRR936002_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936002_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3087370
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72600.2351516015249225No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52000.1684281443429197No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38100.12340600575894693No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA12200.042.20901100-101
GGTATCA74100.035.254311
GCGTATC3800.032.89914100-101
CGGTATC9600.032.866505100-101
GTATCAA148950.031.5211751
CCGTACA6700.029.76589100-101
TCGCGAA400.005367415529.74612448-49
CCTGTCG6900.029.32998396-97
CGTACAT18050.028.364174100-101
AGTATCA34850.027.75872100-101
TACGCAG9350.027.696604100-101
ACCGTAC3050.027.32473698-99
ACGTATC5300.026.957787100-101
CCGTATC5650.025.814667100-101
CGTATAG3900.025.186523100-101
AGGTATC21850.024.793556100-101
GGGTATC15500.024.580864100-101
TCGAGTA5450.024.57734100-101
CGATCTA2600.024.04168100-101
TCGTATC5950.024.01282100-101
Produced by FastQC (version 0.11.5)