FastQCFastQC Report
Thu 26 May 2016
SRR936001_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936001_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1185408
Sequences flagged as poor quality0
Sequence length77
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100680.8493278263686427No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45010.37970049130763417No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAC657.3315075E-427.30446210
CGGCTAT750.001688186423.66586356
CTCGCGC750.001689015823.66386836
TCGCGCT750.001689015823.66386837
GACGTAT1153.6973837E-521.60522839
CGCGGAA850.003491557420.88252659
CGCAGCG1309.503752E-519.1107064
TCGCGCA1351.2685974E-418.40445539
GCGACGA1602.3064511E-517.75164617
CGTGGCG1602.3106388E-517.74790210
CGAACGT1653.0130035E-517.20790730
ACGCCCC1905.3618296E-616.8116722
GTTCGCG1502.8379858E-416.56470737
CGCTGTA1300.002081903616.38682646
TAAGGCG3055.7298166E-1016.29659317
TTCGCGC1553.645888E-416.02900938
ATTACGA1553.6470595E-416.0283341
ACTTTCG2452.1060441E-715.94092648
TACGACA1350.002681395515.77724455
GTTACGG1604.637487E-415.53006912
Produced by FastQC (version 0.11.5)