##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936001_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1185408 Sequences flagged as poor quality 0 Sequence length 77 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.59526340298024 40.0 38.0 41.0 35.0 41.0 2 38.483722903844075 40.0 38.0 41.0 35.0 41.0 3 38.39997705431379 40.0 38.0 41.0 35.0 41.0 4 38.30246379305691 40.0 38.0 41.0 34.0 41.0 5 38.225151171579746 40.0 38.0 41.0 34.0 41.0 6 38.099435806068456 40.0 38.0 41.0 34.0 41.0 7 37.92512957563978 40.0 38.0 41.0 34.0 41.0 8 37.72530892317244 40.0 37.0 41.0 34.0 41.0 9 37.62191836059821 40.0 37.0 41.0 33.0 41.0 10-11 37.78325859113487 40.0 38.0 41.0 34.0 41.0 12-13 37.94954859423928 40.0 38.0 41.0 34.0 41.0 14-15 37.89439416639672 40.0 38.0 41.0 34.0 41.0 16-17 37.720162593807366 40.0 38.0 41.0 33.5 41.0 18-19 37.52145885636 40.0 38.0 41.0 33.0 41.0 20-21 37.2333761877767 40.0 37.5 41.0 33.0 41.0 22-23 36.84106105239715 40.0 37.0 41.0 31.5 41.0 24-25 36.51464896474462 40.0 37.0 41.0 31.0 41.0 26-27 35.574258398796026 39.0 36.0 40.0 29.0 40.5 28-29 35.608397699357525 39.0 35.5 40.0 29.5 40.5 30-31 35.96357119236583 40.0 36.0 41.0 30.0 41.0 32-33 35.81490212652521 39.5 35.5 41.0 29.0 41.0 34-35 35.578316073453195 39.0 35.0 41.0 28.5 41.0 36-37 35.31086807242738 39.0 35.0 41.0 28.0 41.0 38-39 35.03829862798294 38.5 35.0 40.5 27.5 41.0 40-41 34.68030079095131 38.0 35.0 40.0 26.5 41.0 42-43 34.32593081875608 37.0 34.5 40.0 26.0 41.0 44-45 33.949581072508366 37.0 34.0 39.5 26.0 41.0 46-47 33.56189767573696 36.0 34.0 39.0 25.0 41.0 48-49 33.11648394476838 36.0 34.0 39.0 23.5 40.5 50-51 32.69510792908433 35.0 33.5 38.0 22.0 40.0 52-53 32.27209914223626 35.0 33.0 37.0 20.5 39.0 54-55 31.83731213219415 35.0 33.0 37.0 18.5 39.0 56-57 31.41307760703488 35.0 33.0 36.0 16.0 38.5 58-59 31.00920526940935 35.0 32.0 36.0 9.0 37.0 60-61 30.670003492468418 35.0 32.0 35.0 6.5 37.0 62-63 30.360060418016413 35.0 32.0 35.0 2.0 36.5 64-65 30.034044818324155 34.5 31.0 35.0 2.0 36.0 66-67 29.6970637957564 34.0 31.0 35.0 2.0 36.0 68-69 29.38694483249649 34.0 31.0 35.0 2.0 35.5 70-71 29.043281722411187 34.0 30.0 35.0 2.0 35.0 72-73 28.665989684564302 34.0 30.0 35.0 2.0 35.0 74-75 28.207160319484935 34.0 29.5 35.0 2.0 35.0 76-77 26.61305516750351 32.5 26.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.590786533665721 1101 2 0.5310695994851145 1101 3 0.5768410817613798 1101 4 0.6601413567482624 1101 5 0.5803869663928083 1101 6 0.6628561797748276 1101 7 0.6880797617873 1101 8 0.6845884676775782 1101 9 0.7229356109847345 1101 10-11 0.7045367176883772 1101 12-13 0.8313340174055242 1101 14-15 0.7702836777294451 1101 16-17 0.8269343101537387 1101 18-19 0.8211185428226031 1101 20-21 0.9476281951865744 1101 22-23 0.9530773987846786 1101 24-25 1.024971150573208 1101 26-27 0.8365087783391161 1101 28-29 0.7728481995220662 1101 30-31 0.8339501042602535 1101 32-33 0.9536922976372821 1101 34-35 0.9236710068195464 1101 36-37 0.9524379035158148 1101 38-39 0.9465439148482275 1101 40-41 0.7938686186956616 1101 42-43 0.8486921696552159 1101 44-45 0.7178029946916737 1101 46-47 0.7230480922599227 1101 48-49 0.7468285732580213 1101 50-51 0.7407406500310572 1101 52-53 0.7441567447901605 1101 54-55 0.7888891878127993 1101 56-57 0.7233180908535992 1101 58-59 0.9248869567649436 1101 60-61 0.7351365926142144 1101 62-63 0.7877388267696439 1101 64-65 0.7622016515136423 1101 66-67 0.6311478484423461 1101 68-69 0.6262106808373673 1101 70-71 0.6642444847989175 1101 72-73 0.6660245043542297 1101 74-75 0.5577068514827381 1101 76-77 0.5381970631533193 1102 1 0.5225530408427588 1102 2 0.5622695994851128 1102 3 0.6823339047278907 1102 4 0.44478314303215427 1102 5 0.5709566633625016 1102 6 0.6733442180523355 1102 7 0.558826811229082 1102 8 0.6867026622549304 1102 9 0.739240555163363 1102 10-11 0.6664958883422898 1102 12-13 0.6470195038489024 1102 14-15 0.6200208388139785 1102 16-17 0.5334542463578842 1102 18-19 0.6181826576551401 1102 20-21 0.6865908746124134 1102 22-23 0.6111781962328422 1102 24-25 0.5493800819926662 1102 26-27 0.38571196813177977 1102 28-29 0.4167505918665668 1102 30-31 0.36515775976264564 1102 32-33 0.426832329535209 1102 34-35 0.5543548983665971 1102 36-37 0.526371555828419 1102 38-39 0.5151805974638606 1102 40-41 0.5752670237993343 1102 42-43 0.519188501393657 1102 44-45 0.5842852913423897 1102 46-47 0.4739106121961285 1102 48-49 0.6655322415195783 1102 50-51 0.7348727074473231 1102 52-53 0.7773631243754906 1102 54-55 0.7848387890887096 1102 56-57 0.7669085214756031 1102 58-59 0.6577883921716428 1102 60-61 0.5795767840017731 1102 62-63 0.6465534998158944 1102 64-65 0.5874769306205003 1102 66-67 0.384520097246174 1102 68-69 0.5301685755742085 1102 70-71 0.5652173715612783 1102 72-73 0.5313380609730487 1102 74-75 0.5383920189468547 1102 76-77 0.613378562355873 1103 1 0.280575470749298 1103 2 0.3846521540022465 1103 3 0.32217191095841713 1103 4 0.3653463829075463 1103 5 0.40118843906343926 1103 6 0.2848769283327144 1103 7 0.33897260562160625 1103 8 0.4265506373328165 1103 9 0.4391670349141421 1103 10-11 0.45756068585007625 1103 12-13 0.3599154540046712 1103 14-15 0.41784139956164523 1103 16-17 0.3723981715915343 1103 18-19 0.5734736233872297 1103 20-21 0.5642363574784568 1103 22-23 0.6287433052671076 1103 24-25 0.6545788358867455 1103 26-27 0.4675213139261629 1103 28-29 0.5922914018354177 1103 30-31 0.885296077519655 1103 32-33 0.7516379370118429 1103 34-35 0.8532570790519571 1103 36-37 0.8781559795044274 1103 38-39 0.8387020927909603 1103 40-41 0.8292190487214484 1103 42-43 0.7527785325463086 1103 44-45 0.822147596638338 1103 46-47 0.6443205810434733 1103 48-49 0.6756861356005786 1103 50-51 0.7964353242697513 1103 52-53 0.799795523129383 1103 54-55 0.7945141784968115 1103 56-57 0.7006075868961652 1103 58-59 0.6875809155361274 1103 60-61 0.8347263167120538 1103 62-63 0.6382936867317852 1103 64-65 0.5307330053868569 1103 66-67 0.6812920598629937 1103 68-69 0.6214558029885424 1103 70-71 0.43264727810333525 1103 72-73 0.5530458491350423 1103 74-75 0.42848298467893997 1103 76-77 0.32474180223126226 1104 1 0.5168374564271687 1104 2 0.5513618072773241 1104 3 0.5413403982343823 1104 4 0.3491935326425448 1104 5 0.4892073127131553 1104 6 0.3252792829874025 1104 7 0.5617696683719373 1104 8 0.6846169479692179 1104 9 0.5570236720464763 1104 10-11 0.4234647195111165 1104 12-13 0.7185805428099385 1104 14-15 0.7119987608918947 1104 16-17 0.7090971035007456 1104 18-19 0.6107417485642372 1104 20-21 0.7498752901968331 1104 22-23 0.7649223520770008 1104 24-25 0.8488846274472124 1104 26-27 0.7019892408590493 1104 28-29 0.9470194230353997 1104 30-31 1.0462493182042039 1104 32-33 1.0580654464183255 1104 34-35 1.1364971061588065 1104 36-37 0.9029605168673811 1104 38-39 1.0661682598015219 1104 40-41 1.092662478344785 1104 42-43 1.075487852043004 1104 44-45 0.950202174459271 1104 46-47 0.9226664563519762 1104 48-49 1.0054225012598366 1104 50-51 0.9575895905642113 1104 52-53 0.9978449425573075 1104 54-55 1.0400283994783237 1104 56-57 0.9921364435535303 1104 58-59 0.9958669636002107 1104 60-61 0.9392975632225529 1104 62-63 1.0181372660496635 1104 64-65 0.9903003072438779 1104 66-67 0.8017519154279888 1104 68-69 0.636121822327457 1104 70-71 0.6065647741586808 1104 72-73 0.5660088401938275 1104 74-75 0.37687123972607495 1104 76-77 0.45063440651171405 1105 1 -0.14787961221846757 1105 2 0.05923286479123391 1105 3 -0.09578854425170391 1105 4 0.05815457160358051 1105 5 0.14960156132168834 1105 6 -0.11710476153950111 1105 7 -0.08853645407703681 1105 8 0.1171679683773803 1105 9 -0.14154311830602495 1105 10-11 -0.08637758104547544 1105 12-13 -0.04987029206946403 1105 14-15 -0.07798732445133538 1105 16-17 -0.16442330466252741 1105 18-19 0.026541841328608484 1105 20-21 0.039137813387924325 1105 22-23 0.06912921664100935 1105 24-25 -0.0659831833134561 1105 26-27 -0.1815288481947519 1105 28-29 0.11243222451962964 1105 30-31 0.2007414332320394 1105 32-33 0.2363098805556163 1105 34-35 0.2591385718359831 1105 36-37 0.39174298439984057 1105 38-39 0.23880508725977734 1105 40-41 0.16543845237075772 1105 42-43 0.21073135853651337 1105 44-45 0.13198325052605497 1105 46-47 0.1286126530124534 1105 48-49 0.18764652723386632 1105 50-51 0.09590127887589972 1105 52-53 0.025428430497932197 1105 54-55 0.03392450337442554 1105 56-57 -0.001956784646843346 1105 58-59 0.15451492278388557 1105 60-61 -0.17936199150842924 1105 62-63 -0.1981730307963474 1105 64-65 -0.08560878366521507 1105 66-67 -0.04002684152933966 1105 68-69 -0.4961416285074236 1105 70-71 -0.5825377304795367 1105 72-73 -0.8415027553534848 1105 74-75 -0.8564079810531489 1105 76-77 -0.725103070297191 1106 1 -0.002063285687853522 1106 2 0.018314497444301026 1106 3 -0.3173871837074884 1106 4 -0.11609712907668523 1106 5 -0.36890184003885906 1106 6 -0.18608435337623064 1106 7 -0.0368697874103745 1106 8 -0.04242386835731082 1106 9 -0.14361794823800267 1106 10-11 -0.2134013905692811 1106 12-13 -0.245125394110282 1106 14-15 -0.20157575982548082 1106 16-17 -0.0532328284720478 1106 18-19 -0.11856019948771745 1106 20-21 -0.2703009621222847 1106 22-23 -0.2046973139712378 1106 24-25 0.10976511600626537 1106 26-27 -0.06669891622196644 1106 28-29 -0.013826278881730047 1106 30-31 0.10366660330005573 1106 32-33 0.0676704247733042 1106 34-35 0.08367938816251552 1106 36-37 0.20233822249508648 1106 38-39 -0.0028445726041681496 1106 40-41 0.07625477890137233 1106 42-43 0.10600346738005584 1106 44-45 -0.04771062702496209 1106 46-47 0.010058231243746718 1106 48-49 -0.07000653399062173 1106 50-51 -0.24100348302886232 1106 52-53 -0.22467361031839062 1106 54-55 -0.06695985036706986 1106 56-57 -0.06408263498697764 1106 58-59 -0.20842725408686036 1106 60-61 -0.2425592704199957 1106 62-63 -0.2057920784153957 1106 64-65 -0.11719041631827665 1106 66-67 -0.13994180751573282 1106 68-69 -0.2704953700040207 1106 70-71 -0.3834050774183133 1106 72-73 -0.4421660206596023 1106 74-75 -0.3456086613252545 1106 76-77 -0.4500690566917491 1107 1 -0.22760398662089187 1107 2 -0.2395281388024486 1107 3 -0.23053847007017225 1107 4 -0.24841362595007155 1107 5 -0.30664979867303543 1107 6 -0.47733752669725504 1107 7 -0.4898400708387669 1107 8 -0.3173735897644505 1107 9 -0.33802988102404186 1107 10-11 -0.37074934590650344 1107 12-13 -0.11904770132072429 1107 14-15 -0.13627059898893634 1107 16-17 -0.07767014782628223 1107 18-19 -0.1875078108424333 1107 20-21 -0.2936372352421941 1107 22-23 -0.24603085061691843 1107 24-25 -0.42773914256306966 1107 26-27 -0.3577432822721036 1107 28-29 -0.284492703771555 1107 30-31 -0.1490561336137688 1107 32-33 -0.011412581611807582 1107 34-35 -0.12849421310351516 1107 36-37 -0.2356329675964588 1107 38-39 -0.07229016182531467 1107 40-41 -0.1823090666691698 1107 42-43 -0.2183662643575559 1107 44-45 -0.10011987828604418 1107 46-47 -0.2003666091285936 1107 48-49 0.02592254846626929 1107 50-51 -0.029323738433127744 1107 52-53 -0.05428275607685151 1107 54-55 -0.20444844839109422 1107 56-57 -0.1276898630155081 1107 58-59 -0.16153697131785805 1107 60-61 0.04386111356558686 1107 62-63 0.16300002647179213 1107 64-65 0.007659160022761569 1107 66-67 0.23967874021870728 1107 68-69 0.050515586882774244 1107 70-71 -0.024071804529189222 1107 72-73 -0.08755563854230175 1107 74-75 0.02008636466575453 1107 76-77 0.138283893535192 1108 1 -0.2998507777729955 1108 2 -0.13957479192300326 1108 3 -0.2735165328218301 1108 4 -0.20370245760427963 1108 5 -0.28151366281092294 1108 6 -0.36763907550374597 1108 7 -0.1884718363445046 1108 8 -0.20501539661539425 1108 9 -0.2842528418135686 1108 10-11 -0.381351685245626 1108 12-13 -0.2950225009243681 1108 14-15 -0.2181837753467235 1108 16-17 -0.1504821100462479 1108 18-19 -0.27792490002186554 1108 20-21 -0.35708740701050345 1108 22-23 -0.25403835110208206 1108 24-25 -0.19898141363183441 1108 26-27 -0.24990967069081904 1108 28-29 -0.23871384727424072 1108 30-31 -0.2903595035627333 1108 32-33 -0.2521102321195201 1108 34-35 -0.21944048747270273 1108 36-37 -0.16187219914374396 1108 38-39 -0.1826103333237299 1108 40-41 -0.17495477413225302 1108 42-43 -0.20139956543211923 1108 44-45 -0.35216689640621723 1108 46-47 -0.31021253816186345 1108 48-49 -0.08573681178192771 1108 50-51 -0.2511040626401808 1108 52-53 -0.2729522296420157 1108 54-55 -0.3076324917386515 1108 56-57 -0.26265329236687407 1108 58-59 -0.24871693797951266 1108 60-61 -0.3692092144071353 1108 62-63 -0.3000803903989002 1108 64-65 -0.26828408767703493 1108 66-67 -0.28604505788509016 1108 68-69 -0.3415383456668408 1108 70-71 -0.4646632966964752 1108 72-73 -0.29368628270237096 1108 74-75 -0.2642043215463836 1108 76-77 -0.1359140390761091 1109 1 0.1402067672964833 1109 2 0.13631120472671654 1109 3 -0.0440477496537639 1109 4 0.03800247145148461 1109 5 -0.09733621133037218 1109 6 0.04586837857650039 1109 7 -0.030938299336028763 1109 8 -0.13641674806447668 1109 9 -0.04332586580306241 1109 10-11 -0.39040271935632376 1109 12-13 -0.2176982684688724 1109 14-15 -0.1314038950107559 1109 16-17 -0.020947064043426167 1109 18-19 -0.1383624692899872 1109 20-21 -0.15040404758250503 1109 22-23 -0.1785446538900075 1109 24-25 -0.2509566911441041 1109 26-27 -0.25365674603693833 1109 28-29 -0.1809860174700404 1109 30-31 -0.0011613229564346739 1109 32-33 -0.10025399315111372 1109 34-35 -0.12849719008548988 1109 36-37 -0.16626333360648005 1109 38-39 0.004542104425361515 1109 40-41 0.1119857789180898 1109 42-43 0.05272036052551954 1109 44-45 0.05848578274288485 1109 46-47 0.005517164202679226 1109 48-49 -0.14843630884896442 1109 50-51 -0.13007274709812577 1109 52-53 -0.034249652037289025 1109 54-55 0.15834476779468787 1109 56-57 0.22907264923973614 1109 58-59 0.14636202034526846 1109 60-61 0.035537471962463485 1109 62-63 0.06437790604029914 1109 64-65 0.11685055879412687 1109 66-67 0.014871284797358442 1109 68-69 -0.1947837585781258 1109 70-71 -0.17899229264838468 1109 72-73 -0.13952745909247355 1109 74-75 -0.20800503265019543 1109 76-77 -0.12279047941317245 1110 1 -0.5797269917570773 1110 2 -0.3947657713380295 1110 3 -0.5746701702517356 1110 4 -0.42652117644625065 1110 5 -0.4402472486179363 1110 6 -0.6346231821106585 1110 7 -0.6277896516886017 1110 8 -0.4954534909643016 1110 9 -0.39823556545603367 1110 10-11 -0.47993312551264466 1110 12-13 -0.6185671302179259 1110 14-15 -0.5279785091892819 1110 16-17 -0.6918008473666433 1110 18-19 -0.8264132673652327 1110 20-21 -0.9580379762433324 1110 22-23 -1.0268733115096182 1110 24-25 -0.8918378479176852 1110 26-27 -0.8035467115748247 1110 28-29 -0.8271506306273224 1110 30-31 -0.7216207243449304 1110 32-33 -0.5379588685087953 1110 34-35 -0.390085851429653 1110 36-37 -0.46957498043890666 1110 38-39 -0.6352637383144568 1110 40-41 -0.5824157797866505 1110 42-43 -0.4376258425346222 1110 44-45 -0.514006803197141 1110 46-47 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-0.9143196673352989 2313 46-47 -0.5064067431757735 2313 48-49 -0.7695553617862032 2313 50-51 -0.7866843173460616 2313 52-53 -0.8239525781038495 2313 54-55 -0.8187083183493016 2313 56-57 -0.7569559413343114 2313 58-59 -0.7524727648531524 2313 60-61 -0.5772166044279778 2313 62-63 -0.4939126158865861 2313 64-65 -0.44024868921421145 2313 66-67 -0.46813444820837447 2313 68-69 -0.6168297715332294 2313 70-71 -0.5639561821577281 2313 72-73 -0.5628503687790172 2313 74-75 -0.41880467526802434 2313 76-77 -0.5909387930160293 2314 1 -0.3260774676318192 2314 2 -0.34011684119285235 2314 3 -0.18458134950940064 2314 4 -0.2666880434085215 2314 5 -0.32010096375613983 2314 6 -0.4434693412696973 2314 7 -0.44749183283871474 2314 8 -0.4252498801179456 2314 9 -0.15456283466714638 2314 10-11 -0.3526193658950021 2314 12-13 -0.583516089371436 2314 14-15 -0.5043588222029669 2314 16-17 -1.0503762621166928 2314 18-19 -0.8105698847177365 2314 20-21 -0.7815989558960652 2314 22-23 -0.6131675664790208 2314 24-25 -0.7443453417361496 2314 26-27 -0.6233863369529686 2314 28-29 -0.6229205945741114 2314 30-31 -0.7320015622712504 2314 32-33 -0.7169744501258037 2314 34-35 -0.6116552711249312 2314 36-37 -0.7518284441715792 2314 38-39 -0.7371651652480082 2314 40-41 -0.5626245016243914 2314 42-43 -0.640309803691089 2314 44-45 -0.5265587764542232 2314 46-47 -0.49987921831234416 2314 48-49 -0.4205253855990634 2314 50-51 -0.47213746204420204 2314 52-53 -0.47902331630248085 2314 54-55 -0.4368866346400999 2314 56-57 -0.24614910564303827 2314 58-59 -0.24918448918429092 2314 60-61 -0.33801643633720957 2314 62-63 -0.26811768662478386 2314 64-65 -0.25920442531170096 2314 66-67 -0.22776464851654055 2314 68-69 -0.16190261086646984 2314 70-71 -0.05249449284550067 2314 72-73 0.1174024677527079 2314 74-75 0.10164286640448239 2314 76-77 -0.05432652238988922 2315 1 -0.5492099221202054 2315 2 -0.5908816200270834 2315 3 -0.39598949183940846 2315 4 -0.5505794585938588 2315 5 -0.3753081966194287 2315 6 -0.3585582209720499 2315 7 -0.5147724660970212 2315 8 -0.4343177532825564 2315 9 -0.4642745306541656 2315 10-11 -0.5022163067796654 2315 12-13 -0.606627108617225 2315 14-15 -0.702908881149888 2315 16-17 -0.5949179306972212 2315 18-19 -0.46715356637376715 2315 20-21 -0.5529607062367248 2315 22-23 -0.32480843430012385 2315 24-25 -0.35225532902811096 2315 26-27 -0.34572109419884356 2315 28-29 -0.4546665716384055 2315 30-31 -0.4247656368046506 2315 32-33 -0.3525450869056215 2315 34-35 -0.3214362488782072 2315 36-37 -0.6142268181553234 2315 38-39 -0.46357568076559375 2315 40-41 -0.40239223545992786 2315 42-43 -0.3687421219125824 2315 44-45 -0.3701324141680047 2315 46-47 -0.3933728566385639 2315 48-49 -0.361843729573458 2315 50-51 -0.3769726403394884 2315 52-53 -0.41456677625685145 2315 54-55 -0.45270086764118744 2315 56-57 -0.369332851604792 2315 58-59 -0.22797240276964104 2315 60-61 -0.31186314373083945 2315 62-63 -0.290355985278957 2315 64-65 -0.26019389142105354 2315 66-67 -0.22868712949276215 2315 68-69 -0.3452746041909194 2315 70-71 -0.3557943718894876 2315 72-73 -1.5851349290901844 2315 74-75 -2.395793708243751 2315 76-77 -1.8682843102728555 2316 1 -1.966246959157246 2316 2 -1.7783421652207707 2316 3 -1.3190778599779946 2316 4 -1.189240386379609 2316 5 -2.021466866049259 2316 6 -1.6440675466535453 2316 7 -1.6497849534767965 2316 8 -1.7013914553921268 2316 9 -1.1776535624836981 2316 10-11 -1.3874099940106532 2316 12-13 -1.4077334373275718 2316 14-15 -1.436402690597788 2316 16-17 -2.471722224230355 2316 18-19 -2.2925938129330916 2316 20-21 -1.7011503018581706 2316 22-23 -1.7860688625906818 2316 24-25 -1.810172859183801 2316 26-27 -1.4036056789270432 2316 28-29 -1.5134141140157809 2316 30-31 -1.5566775343550034 2316 32-33 -1.9499206116412608 2316 34-35 -1.823574513398107 2316 36-37 -1.6885931500539328 2316 38-39 -1.7688899907714344 2316 40-41 -1.6378388585536143 2316 42-43 -2.0317056162534044 2316 44-45 -1.7058558851281944 2316 46-47 -1.2600187995685843 2316 48-49 -1.5039501114215952 2316 50-51 -1.827880233729143 2316 52-53 -2.7378368756245095 2316 54-55 -2.080361210911285 2316 56-57 -2.072220890289099 2316 58-59 -2.2023998431224747 2316 60-61 -1.9100702748217557 2316 62-63 -1.8489053237135167 2316 64-65 -1.8389701282030302 2316 66-67 -1.912091667459709 2316 68-69 -1.987102012661083 2316 70-71 -1.803606157850485 2316 72-73 -1.5347560566740093 2316 74-75 -1.7287609222296183 2316 76-77 -1.8192567317617758 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13629.0 3 1312.0 4 2225.0 5 2910.0 6 3282.0 7 3583.0 8 4890.0 9 5442.0 10 9154.0 11 11915.0 12 7815.0 13 4550.0 14 3843.0 15 3609.0 16 3551.0 17 3504.0 18 3645.0 19 3775.0 20 3817.0 21 4121.0 22 4529.0 23 5053.0 24 5682.0 25 6602.0 26 7918.0 27 9192.0 28 11318.0 29 13619.0 30 17341.0 31 22224.0 32 30230.0 33 42477.0 34 66964.0 35 102317.0 36 163412.0 37 249504.0 38 250041.0 39 75670.0 40 743.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 21.04483441982187 26.485741788905415 31.239828670305076 21.22959512096764 2 20.766264259252853 26.727034809408384 31.19668467361803 21.31001625772073 3 20.757537791254414 26.890954819233908 30.761747834443216 21.58975955506846 4 20.754785431768788 26.827609713954022 30.9909859744327 21.42661887984449 5 20.765269362058987 27.149657212376464 31.0414736746192 21.043599750945344 6 21.094912002591705 26.855892801853344 30.817020565771745 21.232174629783206 7 21.031951935837956 26.5934837321103 30.927087894611176 21.44747643744057 8 20.73059299089037 27.004317309144476 30.66344141090034 21.601648289064816 9 20.65926389033024 26.824391194627882 30.76370766542556 21.75263724961632 10-11 21.186325915632352 26.969036171364376 30.60144422212013 21.243193690883142 12-13 21.16855910081114 26.816542726151365 30.480507843941258 21.53439032909624 14-15 20.865636372149567 27.062125181754027 30.607905261386225 21.46433318471018 16-17 21.10620021495833 26.959807516117657 30.52914507662742 21.404847192296593 18-19 21.01567212976439 26.979560842877937 30.24780105043346 21.756965976924214 20-21 21.088236770728344 27.2049067351589 30.025655056147066 21.681201437965687 22-23 21.231573520713678 27.33345820856357 29.511349724051055 21.923618546671694 24-25 21.20973573258882 27.50967979271027 29.25946738148213 22.021117093218788 26-27 21.37314437810958 27.734920982476037 28.95573845809196 21.936196181322426 28-29 21.38989025006517 27.861922112895048 28.85379695629322 21.894390680746557 30-31 21.364116858497752 27.74634258278911 28.8423655502134 22.047175008499735 32-33 21.250603746285247 27.9918080329366 28.66585253193791 22.091735688840238 34-35 21.191236402583506 27.70432877368778 28.939765348465503 22.164669475263217 36-37 21.301991057116343 27.883194128068844 28.945836497089346 21.86897831772547 38-39 21.226880717071104 27.87053707775301 29.045421027853664 21.857161177322222 40-41 21.109563910066232 27.865311110445546 28.911338068302854 22.11378691118536 42-43 21.07486511455244 27.980409444301486 28.77158272622578 22.173142714920292 44-45 21.29616036852363 27.829734997089272 28.854100753414833 22.02000388097227 46-47 21.241717286180894 27.823632302773742 28.750857487117653 22.183792923927708 48-49 21.138107454644327 27.918876810356064 28.64633102436337 22.29668471063624 50-51 21.01953397940274 28.194735580736737 28.428868230870236 22.35686220899029 52-53 20.997271667793274 28.04165158193478 28.58250778709726 22.37856896317469 54-55 20.974834687434473 27.936969218300344 28.695637132115465 22.39255896214972 56-57 21.032159700369853 28.11876025411863 28.431071862044583 22.418008183466934 58-59 21.142163126506297 27.992037012116178 28.428955138375688 22.43684472300184 60-61 20.99355634548518 27.9651399725109 28.42198385584844 22.619319826155483 62-63 20.940330502013904 27.953868605008793 28.47018043913085 22.635620453846457 64-65 21.10465523453367 27.785981222455764 28.508259368732276 22.60110417427829 66-67 20.972662586833575 27.89981415031253 28.495334772033527 22.632188490820376 68-69 21.083321588706255 27.785185566514393 28.484968424534934 22.64652442024442 70-71 20.854335187911037 27.58411432661274 28.61186884714589 22.94968163833034 72-73 20.950798980985287 27.528847107690947 28.588850358069273 22.931503553254494 74-75 20.86103687619276 27.480000894899536 28.53252094465933 23.126441284248376 76-77 20.794526365379802 27.36948173464033 28.766258190041466 23.069733709938397 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 11389.0 1 6751.5 2 1962.0 3 1832.5 4 1855.0 5 1898.0 6 1912.5 7 1991.5 8 2131.0 9 2163.0 10 2187.5 11 2305.5 12 2520.0 13 2641.0 14 2739.0 15 3007.0 16 3388.0 17 3599.0 18 3838.5 19 4302.0 20 4962.0 21 5998.0 22 6598.0 23 7125.0 24 8600.5 25 10920.0 26 12291.0 27 13855.5 28 17218.0 29 20650.0 30 22284.0 31 24806.0 32 29369.0 33 33678.5 34 37861.0 35 39775.0 36 41185.0 37 44238.5 38 46108.0 39 46334.0 40 46805.0 41 48342.5 42 50138.5 43 50868.0 44 50886.0 45 51755.0 46 52603.0 47 52634.5 48 52669.0 49 51931.5 50 50388.5 51 48037.5 52 46492.0 53 44761.0 54 41362.0 55 37657.0 56 33526.0 57 31432.0 58 29367.5 59 24568.5 60 19921.5 61 18009.0 62 15930.5 63 11657.0 64 8068.5 65 6675.0 66 5635.5 67 3978.0 68 2910.5 69 2035.0 70 1609.0 71 1395.5 72 1064.0 73 848.0 74 750.0 75 676.5 76 469.5 77 258.0 78 180.0 79 164.0 80 134.5 81 97.0 82 60.5 83 48.0 84 43.0 85 39.5 86 29.5 87 18.0 88 19.0 89 22.5 90 21.5 91 18.0 92 15.0 93 13.5 94 14.0 95 17.5 96 22.0 97 17.0 98 9.5 99 23.0 100 39.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.007676681783824642 2 0.010544892560198683 3 0.01240079365079365 4 0.012147716229348881 5 0.010629251700680272 6 0.007507963502861462 7 0.013413103336572724 8 0.015100286146204513 9 0.014931567865241333 10-11 0.017082725947521867 12-13 0.013666180758017493 14-15 0.00704398823021272 16-17 0.005230266709858547 18-19 0.01543772270813087 20-21 0.022059915235935645 22-23 0.01898080660835763 24-25 0.017462342079689018 26-27 0.021891196954972464 28-29 0.006495653817082388 30-31 0.006495653817082388 32-33 0.008478093618399742 34-35 0.009996558147068351 36-37 0.009110787172011662 38-39 0.011978997948385703 40-41 0.009574762444660403 42-43 0.012063357088867294 44-45 0.011641561386459345 46-47 0.02184901738473167 48-49 0.020878887269193392 50-51 0.03340621963070943 52-53 0.036696226109491414 54-55 0.02568735827664399 56-57 0.01801067649281935 58-59 0.014720670014037361 60-61 0.017799778641615378 62-63 0.02290350664075154 64-65 0.022692608789547566 66-67 0.026193513119533527 68-69 0.03956443688586546 70-71 0.04994061116510096 72-73 0.078496180218119 74-75 0.07739951139185833 76-77 0.08153310927545622 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 1185408.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.71593992358526 #Duplication Level Percentage of deduplicated Percentage of total 1 79.13039750857723 50.41867653785928 2 12.237935665126267 15.595031472557732 3 3.9481867371102153 7.546872868464318 4 1.71801461129238 4.378596630437878 5 0.8878693166291702 2.8285714019169457 6 0.5037561775660517 1.92583790075601 7 0.30600795760956545 1.3648309250233068 8 0.22426893986911145 1.1431605039541166 9 0.16446270440606126 0.9431006214246264 >10 0.8215528525428527 9.293684773101822 >50 0.04218686930100544 1.8250301431610172 >100 0.015095822566442253 1.5073931684178998 >500 0.0 0.0 >1k 1.3241870182774322E-4 0.3797575640892078 >5k 0.0 0.0 >10k+ 1.3241870182774322E-4 0.8494554888358463 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10068 0.8493278263686427 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4501 0.37970049130763417 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.3743656192635786E-4 0.0 3 0.0 0.0 0.0 5.061548428895368E-4 0.0 4 8.435914048158947E-5 0.0 0.0 9.279505452974841E-4 0.0 5 8.435914048158947E-5 0.0 0.0 0.0022776967930029156 0.0 6 8.435914048158947E-5 0.0 0.0 0.004217957024079473 0.0 7 8.435914048158947E-5 0.0 0.0 0.007676681783824641 0.0 8 8.435914048158947E-5 0.0 0.0 0.011388483965014577 0.0 9 8.435914048158947E-5 0.0 0.0 0.016450032393909946 0.0 10 8.435914048158947E-5 0.0 0.0 0.02142722168232372 0.0 11 3.3743656192635786E-4 0.0 0.0 0.02817595292085088 0.0 12 5.061548428895368E-4 0.0 0.0 0.03964879602634705 0.0 13 5.061548428895368E-4 0.0 0.0 0.052555744520030236 0.0 14 6.748731238527157E-4 0.0 0.0 0.07170526940935104 0.0 15 7.592322643343052E-4 0.0 0.0 0.0896737663319296 0.0 16 7.592322643343052E-4 0.0 0.0 0.10907636864269517 8.435914048158947E-5 17 7.592322643343052E-4 0.0 0.0 0.1273823021272001 8.435914048158947E-5 18 8.435914048158947E-4 0.0 0.0 0.1465318270165209 8.435914048158947E-5 19 0.0010123096857790736 0.0 0.0 0.16627186588921283 8.435914048158947E-5 20 0.0010123096857790736 0.0 0.0 0.18921755210020516 8.435914048158947E-5 21 0.0010123096857790736 0.0 0.0 0.21595939963286903 8.435914048158947E-5 22 0.001096668826260663 0.0 0.0 0.24607561278479645 8.435914048158947E-5 23 0.0011810279667422525 0.0 0.0 0.2761918259367239 8.435914048158947E-5 24 0.0011810279667422525 0.0 0.0 0.30875445416261743 1.6871828096317893E-4 25 0.0011810279667422525 0.0 0.0 0.34140144152899254 1.6871828096317893E-4 26 0.0011810279667422525 0.0 0.0 0.3757356117049995 1.6871828096317893E-4 27 0.0011810279667422525 0.0 0.0 0.4088887539142641 1.6871828096317893E-4 28 0.0011810279667422525 0.0 0.0 0.4427167692473815 1.6871828096317893E-4 29 0.0011810279667422525 0.0 0.0 0.4787381222330202 1.6871828096317893E-4 30 0.0011810279667422525 0.0 0.0 0.5173746085735882 1.6871828096317893E-4 31 0.0011810279667422525 0.0 0.0 0.5665559874743549 1.6871828096317893E-4 32 0.001265387107223842 0.0 0.0 0.6175089083252349 1.6871828096317893E-4 33 0.001265387107223842 0.0 0.0 0.6665215689450383 1.6871828096317893E-4 34 0.001265387107223842 0.0 0.0 0.7216924468199979 1.6871828096317893E-4 35 0.001265387107223842 0.0 0.0 0.7794784580498866 1.6871828096317893E-4 36 0.001265387107223842 0.0 0.0 0.8407231940395206 1.6871828096317893E-4 37 0.0013497462477054314 0.0 0.0 0.9062702461937155 1.6871828096317893E-4 38 0.0013497462477054314 0.0 0.0 0.9686960101500918 1.6871828096317893E-4 39 0.0013497462477054314 0.0 0.0 1.035002294568621 1.6871828096317893E-4 40 0.0015184645286686104 0.0 0.0 1.1073824371018248 1.6871828096317893E-4 41 0.0015184645286686104 0.0 0.0 1.1816184807256236 1.6871828096317893E-4 42 0.0016028236691501998 0.0 0.0 1.2646278749595077 1.6871828096317893E-4 43 0.0016028236691501998 0.0 0.0 1.351686507936508 1.6871828096317893E-4 44 0.0016028236691501998 0.0 0.0 1.4435536119209589 1.6871828096317893E-4 45 0.0016028236691501998 0.0 0.0 1.5460499676060901 1.6871828096317893E-4 46 0.0016028236691501998 0.0 0.0 1.6552950545297485 1.6871828096317893E-4 47 0.0017715419501133788 0.0 0.0 1.7636121909081093 1.6871828096317893E-4 48 0.0017715419501133788 0.0 0.0 1.8778344671201814 1.6871828096317893E-4 49 0.0017715419501133788 0.0 0.0 1.998721115430299 1.6871828096317893E-4 50 0.0017715419501133788 0.0 0.0 2.1171613486664507 1.6871828096317893E-4 51 0.0017715419501133788 0.0 0.0 2.246315192743764 1.6871828096317893E-4 52 0.0017715419501133788 0.0 0.0 2.374709804556743 1.6871828096317893E-4 53 0.0017715419501133788 0.0 0.0 2.520651117589893 1.6871828096317893E-4 54 0.0017715419501133788 0.0 0.0 2.6783183511499837 1.6871828096317893E-4 55 0.0017715419501133788 0.0 0.0 2.8450963718820863 1.6871828096317893E-4 56 0.002024619371558147 0.0 0.0 3.020394665802829 1.6871828096317893E-4 57 0.002024619371558147 0.0 0.0 3.2005014307310224 1.6871828096317893E-4 58 0.002024619371558147 0.0 0.0 3.3840669204189613 1.6871828096317893E-4 59 0.002024619371558147 0.0 0.0 3.572356521973869 1.6871828096317893E-4 60 0.002024619371558147 0.0 0.0 3.767985368750675 1.6871828096317893E-4 61 0.0022776967930029156 0.0 0.0 3.976099368318756 1.6871828096317893E-4 62 0.0022776967930029156 0.0 0.0 4.189527993737177 1.6871828096317893E-4 63 0.0022776967930029156 0.0 0.0 4.416707159054098 1.6871828096317893E-4 64 0.0022776967930029156 0.0 0.0 4.651563006154843 1.6871828096317893E-4 65 0.0022776967930029156 0.0 0.0 4.892155274808336 1.6871828096317893E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGTTAC 65 7.3315075E-4 27.304462 10 CGGCTAT 75 0.0016881864 23.665863 56 CTCGCGC 75 0.0016890158 23.663868 36 TCGCGCT 75 0.0016890158 23.663868 37 GACGTAT 115 3.6973837E-5 21.605228 39 CGCGGAA 85 0.0034915574 20.882526 59 CGCAGCG 130 9.503752E-5 19.110706 4 TCGCGCA 135 1.2685974E-4 18.404455 39 GCGACGA 160 2.3064511E-5 17.751646 17 CGTGGCG 160 2.3106388E-5 17.747902 10 CGAACGT 165 3.0130035E-5 17.207907 30 ACGCCCC 190 5.3618296E-6 16.811672 2 GTTCGCG 150 2.8379858E-4 16.564707 37 CGCTGTA 130 0.0020819036 16.386826 46 TAAGGCG 305 5.7298166E-10 16.296593 17 TTCGCGC 155 3.645888E-4 16.029009 38 ATTACGA 155 3.6470595E-4 16.028334 1 ACTTTCG 245 2.1060441E-7 15.940926 48 TACGACA 135 0.0026813955 15.777244 55 GTTACGG 160 4.637487E-4 15.530069 12 >>END_MODULE