FastQCFastQC Report
Thu 26 May 2016
SRR936001_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936001_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1185408
Sequences flagged as poor quality0
Sequence length125
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92700.7820092322643344No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71200.6006370802289169No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54380.4587450059388835No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14790.12476716877227081No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14670.12375485908649173No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA7000.052.714596100-101
GGTATCA60850.047.3293341
CGGTATC7400.045.039497100-101
TACGCAG6500.041.661537100-101
CTACGCG455.154723E-639.6701296-97
CGTACAT9100.039.568645100-101
CCGTATC3100.039.357674100-101
ACGTATC2050.039.19378100-101
TACGGTA1150.038.81099798-99
CCGTACA3800.038.372467100-101
TCGTATC2550.037.34367100-101
AGTATCA19750.037.216633100-101
ACCGTAC1600.037.19387498-99
AGGTATC13300.036.918118100-101
CGAGTAC5700.036.545204100-101
GCGTATC1900.036.023132100-101
TCGAGTA2150.034.602604100-101
CCGGTAT2350.034.19032100-101
TGTATCA17250.033.98477100-101
GTATCAA123750.033.1779441
Produced by FastQC (version 0.11.5)