FastQCFastQC Report
Thu 26 May 2016
SRR936000_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936000_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1201705
Sequences flagged as poor quality0
Sequence length77
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101600.8454654012424014No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44450.3698911130435506No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAC1102.780871E-829.0396674
CGATTGC1051.8220444E-523.67771568
GTCGGGT1505.548991E-721.30905566
CTCGCGC900.00486824219.71747236
CGTAGAG1159.1665785E-418.52113215
GTACGAA1000.00892370317.75237361
TCGCGCT1000.00893869217.74720237
GCGGTTA1653.0129586E-517.2079753
CAGCGTA1250.00160429517.03802312
TTAGGCG1502.8397815E-416.5633646
TCGGGTG2159.421801E-716.5186567
GGCGGTT1806.3482206E-515.7739772
TAGGCGT1604.6419018E-415.5281547
CGGGTGC2301.9027939E-615.44198968
TTGCGCG1400.003420444415.2112545
TCGCCCG1655.858524E-415.05948458
ATCGATT1655.858524E-415.05948423
CGCTCCG1450.00431599614.68856125
AACTCGA1450.0043171914.6879513
TACGTAG1450.00432077314.6861162
Produced by FastQC (version 0.11.5)