##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936000_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1201705 Sequences flagged as poor quality 0 Sequence length 77 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.55937355673814 40.0 38.0 41.0 35.0 41.0 2 38.448474459205876 40.0 38.0 41.0 35.0 41.0 3 38.334032894928455 40.0 38.0 41.0 34.0 41.0 4 38.244262110917404 40.0 38.0 41.0 34.0 41.0 5 38.16672311424185 40.0 38.0 41.0 34.0 41.0 6 38.052809133689216 40.0 38.0 41.0 34.0 41.0 7 37.88768208503751 40.0 38.0 41.0 34.0 41.0 8 37.734463116987946 40.0 37.0 41.0 34.0 41.0 9 37.85467648050062 40.0 38.0 41.0 34.0 41.0 10-11 38.04966817979454 40.0 38.0 41.0 34.0 41.0 12-13 37.98910007031676 40.0 38.0 41.0 34.0 41.0 14-15 37.84656675307168 40.0 38.0 41.0 34.0 41.0 16-17 37.67928609766956 40.0 38.0 41.0 33.0 41.0 18-19 37.47340362235324 40.0 38.0 41.0 33.0 41.0 20-21 37.180925019035456 40.0 37.0 41.0 33.0 41.0 22-23 36.78647088927815 40.0 37.0 41.0 31.5 41.0 24-25 36.459080223515755 40.0 37.0 41.0 31.0 41.0 26-27 35.496275708264506 39.0 36.0 40.0 29.0 40.5 28-29 35.514046708634815 39.0 35.5 40.0 28.5 40.5 30-31 35.894833174531186 39.5 36.0 41.0 29.5 41.0 32-33 35.77665650055546 39.0 35.5 41.0 29.0 41.0 34-35 35.5497909220649 39.0 35.0 41.0 28.0 41.0 36-37 35.27073283376536 39.0 35.0 41.0 28.0 41.0 38-39 34.97382219429893 38.5 35.0 40.0 27.0 41.0 40-41 34.63745012295031 38.0 35.0 40.0 26.0 41.0 42-43 34.278447289476205 37.0 34.5 40.0 26.0 41.0 44-45 33.90909499419574 37.0 34.0 39.5 25.5 41.0 46-47 33.50345966772211 36.0 34.0 39.0 24.5 41.0 48-49 33.089258179004 36.0 34.0 39.0 23.5 40.0 50-51 32.64621142460088 35.0 33.5 37.5 21.5 40.0 52-53 32.19744612862557 35.0 33.0 37.0 20.0 39.0 54-55 31.766421459509615 35.0 33.0 37.0 18.0 39.0 56-57 31.35911475778165 35.0 32.5 36.0 14.5 38.5 58-59 30.950114212722756 35.0 32.0 36.0 7.5 37.0 60-61 30.602967450414205 35.0 32.0 35.0 4.5 37.0 62-63 30.27171643623019 35.0 31.5 35.0 2.0 36.5 64-65 29.939361989839437 34.0 31.0 35.0 2.0 36.0 66-67 29.641842215851646 34.0 31.0 35.0 2.0 36.0 68-69 29.33729076603659 34.0 31.0 35.0 2.0 35.0 70-71 28.964495029978238 34.0 30.0 35.0 2.0 35.0 72-73 28.588281233747054 34.0 30.0 35.0 2.0 35.0 74-75 28.152395554649438 34.0 29.0 35.0 2.0 35.0 76-77 26.50065531890106 32.5 26.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6483051512121634 1101 2 0.5958835978612882 1101 3 0.5972864128946256 1101 4 0.5488774259407734 1101 5 0.6633255492475811 1101 6 0.708878470753433 1101 7 0.7594480618372188 1101 8 0.7193082902014751 1101 9 0.7090373545859947 1101 10-11 0.7582250379627382 1101 12-13 0.8346880277126019 1101 14-15 0.7654141012935085 1101 16-17 0.7611838320106727 1101 18-19 0.8604594740957197 1101 20-21 0.823930445259343 1101 22-23 0.8577029981159896 1101 24-25 0.914213778219569 1101 26-27 0.9577513541278861 1101 28-29 1.0507694179687732 1101 30-31 0.9693897298076664 1101 32-33 0.8940100011393355 1101 34-35 1.0631738924307044 1101 36-37 0.9907714584180312 1101 38-39 1.095949619277107 1101 40-41 1.0298455613553514 1101 42-43 0.9815587058450959 1101 44-45 1.0626865240035102 1101 46-47 0.9880245795149065 1101 48-49 1.018379565643606 1101 50-51 0.9620469590275746 1101 52-53 0.951228647454009 1101 54-55 0.9897155874345458 1101 56-57 0.9998514485885472 1101 58-59 0.9664343755776699 1101 60-61 0.87137446274836 1101 62-63 0.9625009953218431 1101 64-65 1.0474549479407855 1101 66-67 1.0835816805880896 1101 68-69 1.0043769815793517 1101 70-71 1.0215686435846543 1101 72-73 1.0482353494282037 1101 74-75 1.245436862342057 1101 76-77 1.1523683544817729 1102 1 0.408226234552032 1102 2 0.4744356547218729 1102 3 0.581485784697719 1102 4 0.36559797978721065 1102 5 0.3183857168589981 1102 6 0.3913496092104367 1102 7 0.5285960755450105 1102 8 0.46370087175868946 1102 9 0.561755851341232 1102 10-11 0.5138439871857869 1102 12-13 0.5963594834966699 1102 14-15 0.6947206171068245 1102 16-17 0.6558420827451812 1102 18-19 0.6623255002659789 1102 20-21 0.5898901982239053 1102 22-23 0.5646683965505659 1102 24-25 0.599970612513026 1102 26-27 0.5079529121669069 1102 28-29 0.597027449157224 1102 30-31 0.6335211622534374 1102 32-33 0.6028139824871417 1102 34-35 0.6028922415919595 1102 36-37 0.6585016473844547 1102 38-39 0.8018968498143053 1102 40-41 0.7906487493854826 1102 42-43 0.7623239806697057 1102 44-45 0.5779642313154127 1102 46-47 0.6273290889724521 1102 48-49 0.6654448667584063 1102 50-51 0.5764962950852492 1102 52-53 0.576262086716774 1102 54-55 0.6187577024563851 1102 56-57 0.7004345601465438 1102 58-59 0.6631316141864119 1102 60-61 0.6043925061133173 1102 62-63 0.5634743084839968 1102 64-65 0.580158546927926 1102 66-67 0.5969533499222095 1102 68-69 0.4246145387143656 1102 70-71 0.4252814395221307 1102 72-73 0.35154272313156554 1102 74-75 0.22739234430560984 1102 76-77 0.15746207666376932 1103 1 0.18727792888019223 1103 2 0.09183785520360743 1103 3 0.15608981562662194 1103 4 0.3526871719021685 1103 5 0.3013824264745537 1103 6 0.28625456356213874 1103 7 0.29243702009567585 1103 8 0.20275876897843403 1103 9 0.384669237822969 1103 10-11 0.41177819850951636 1103 12-13 0.41067229935991634 1103 14-15 0.43369384842120695 1103 16-17 0.4705716925215171 1103 18-19 0.5495677445719735 1103 20-21 0.552449152430647 1103 22-23 0.39237981474310146 1103 24-25 0.538813309796474 1103 26-27 0.4900690963666463 1103 28-29 0.47333977069040856 1103 30-31 0.6363106810302526 1103 32-33 0.6961077892207257 1103 34-35 0.713094572310645 1103 36-37 0.6622016959856509 1103 38-39 0.7452841752391492 1103 40-41 0.7292169508915265 1103 42-43 0.735906138681834 1103 44-45 0.5817528077755512 1103 46-47 0.4703932580048118 1103 48-49 0.39525873247887233 1103 50-51 0.5153262182145539 1103 52-53 0.625868880427852 1103 54-55 0.5744756515094878 1103 56-57 0.5306372354703193 1103 58-59 0.6748793798977282 1103 60-61 0.4138202477735753 1103 62-63 0.46573895806221444 1103 64-65 0.47148376738606146 1103 66-67 0.33129877255658613 1103 68-69 0.18544553606091085 1103 70-71 0.22726749371068777 1103 72-73 0.2876223411665322 1103 74-75 0.2104033134420682 1103 76-77 0.46919391924340914 1104 1 0.29916501979651855 1104 2 0.4260958486469235 1104 3 0.40192975008394427 1104 4 0.3208811164442835 1104 5 0.3487464211992801 1104 6 0.24626472315951986 1104 7 0.25895981946293745 1104 8 0.32409657800454994 1104 9 0.30306908916947606 1104 10-11 0.4129615744158244 1104 12-13 0.5569940304008583 1104 14-15 0.6052184999240922 1104 16-17 0.5459017286110139 1104 18-19 0.6137454506507254 1104 20-21 0.6418698985215556 1104 22-23 0.7251732937777717 1104 24-25 0.8949937717565106 1104 26-27 0.7227814916787523 1104 28-29 0.7759521868767436 1104 30-31 0.8153627254380709 1104 32-33 0.8209492717497184 1104 34-35 0.8438834010973011 1104 36-37 0.793841179897413 1104 38-39 0.8278864076960488 1104 40-41 0.7820432374621475 1104 42-43 0.9373077890734365 1104 44-45 0.906119371358109 1104 46-47 0.7758288235939403 1104 48-49 0.6882650804173309 1104 50-51 0.6568866937609243 1104 52-53 0.7431033342529645 1104 54-55 0.48301484137209627 1104 56-57 0.4479894537496314 1104 58-59 0.45778827051072213 1104 60-61 0.35630510188923736 1104 62-63 0.3413565879801759 1104 64-65 0.27940403023245963 1104 66-67 0.16669821245146466 1104 68-69 -0.049827874149123375 1104 70-71 0.111829671773922 1104 72-73 -0.12035638159591144 1104 74-75 -0.25029189501670146 1104 76-77 -0.17692419753887023 1105 1 -0.05593695265991272 1105 2 0.15718596339584678 1105 3 0.07520038144226504 1105 4 -0.026532247641888773 1105 5 -0.22034697619182708 1105 6 -0.09621271465690029 1105 7 -0.045923948206386456 1105 8 -0.15532828968486712 1105 9 -0.23379005389821828 1105 10-11 0.006920029372800229 1105 12-13 -0.08213006400141865 1105 14-15 -0.053346636118938306 1105 16-17 -0.1823208594126129 1105 18-19 -0.28746903125646384 1105 20-21 -0.32396502190069754 1105 22-23 -0.41824850181793494 1105 24-25 -0.30829018636348593 1105 26-27 -0.39498090436293154 1105 28-29 -0.23964455057886624 1105 30-31 -0.15060498408158907 1105 32-33 -0.2273805763682617 1105 34-35 -0.14597438297566612 1105 36-37 -0.11827935432149417 1105 38-39 -0.26285340702128934 1105 40-41 -0.11444048331228629 1105 42-43 -0.3380692841112989 1105 44-45 -0.5432174009337132 1105 46-47 -0.480101824608262 1105 48-49 -0.3575124655444455 1105 50-51 -0.35341821871789136 1105 52-53 -0.4409429529160178 1105 54-55 -0.6139995572727344 1105 56-57 -0.7611420098494044 1105 58-59 -0.6041847601432231 1105 60-61 -0.4571573199924899 1105 62-63 -0.5488806618738771 1105 64-65 -0.3562304623935617 1105 66-67 -0.49346338350600405 1105 68-69 -0.6452529906685669 1105 70-71 -0.6308447963519122 1105 72-73 -0.968168112974336 1105 74-75 -1.0406986603221426 1105 76-77 -0.9912569263428566 1106 1 0.03500457380292943 1106 2 -0.12520413489758653 1106 3 -0.13167061093948718 1106 4 0.07031140750695641 1106 5 8.886265338503563E-4 1106 6 -0.007851642072523646 1106 7 -0.20534423806146407 1106 8 -0.056886612465227415 1106 9 0.09631959654701916 1106 10-11 -0.1881592687035365 1106 12-13 0.10708353828204054 1106 14-15 0.11125591870628426 1106 16-17 -0.020679419383668574 1106 18-19 0.142921529357821 1106 20-21 0.11891573952164691 1106 22-23 0.07152516197430003 1106 24-25 -0.04703242238833383 1106 26-27 -0.02576494022294895 1106 28-29 0.16709352455831805 1106 30-31 0.29300694781653647 1106 32-33 0.2049761041680327 1106 34-35 0.0802035301482178 1106 36-37 0.15927019441314627 1106 38-39 0.2504496241954186 1106 40-41 0.19081313987611992 1106 42-43 0.30320491235496405 1106 44-45 0.26783156487464055 1106 46-47 0.3350037554033918 1106 48-49 0.12130620417756433 1106 50-51 0.2648228514543689 1106 52-53 0.2519868044660356 1106 54-55 0.23680272015997872 1106 56-57 0.2488468843997218 1106 58-59 0.038539214208018535 1106 60-61 -0.14902298563400862 1106 62-63 -0.15587527483092245 1106 64-65 -0.12093804445551015 1106 66-67 -0.04744939327504483 1106 68-69 -0.04491429039318717 1106 70-71 -0.22741080772902578 1106 72-73 -0.1272215612848555 1106 74-75 -0.24501055603863975 1106 76-77 -0.3982311871320192 1107 1 -0.15380146919183346 1107 2 -0.1707161901014871 1107 3 -0.02298418139729108 1107 4 0.01907585235186815 1107 5 -0.13732948593132477 1107 6 -0.42856549335538574 1107 7 -0.2556149263831955 1107 8 -0.34314983714779856 1107 9 -0.41095820250538395 1107 10-11 -0.3126693265955467 1107 12-13 -0.32610412643987274 1107 14-15 -0.27750845105171607 1107 16-17 -0.290892816390695 1107 18-19 -0.36740855271217754 1107 20-21 -0.48999219641419955 1107 22-23 -0.42495326180122106 1107 24-25 -0.38722963816559997 1107 26-27 -0.4907444039522133 1107 28-29 -0.5618624294584578 1107 30-31 -0.3579421507650693 1107 32-33 -0.24449885059210175 1107 34-35 -0.33145953234662073 1107 36-37 -0.3054671140213614 1107 38-39 -0.2534642811500234 1107 40-41 -0.4380538361486259 1107 42-43 -0.5001189975707732 1107 44-45 -0.39482639921879326 1107 46-47 -0.38103067945495894 1107 48-49 -0.3730115936734535 1107 50-51 -0.3818885294690517 1107 52-53 -0.3698323261776828 1107 54-55 -0.4106257407835656 1107 56-57 -0.3615083517861599 1107 58-59 -0.42710007577013087 1107 60-61 -0.49587571767134264 1107 62-63 -0.45451563855192845 1107 64-65 -0.3807650442077204 1107 66-67 -0.4757179266023712 1107 68-69 -0.5484386823769043 1107 70-71 -0.48815668358032127 1107 72-73 -0.6267297420040379 1107 74-75 -0.8930002916576179 1107 76-77 -0.8048671807946874 1108 1 0.11216114115032383 1108 2 0.3065166551928016 1108 3 0.39271840814320313 1108 4 0.24894846438304796 1108 5 0.1276694917646708 1108 6 0.2853280625735337 1108 7 0.2613224286052045 1108 8 0.22344482375840613 1108 9 0.05550755919999517 1108 10-11 0.0856336487535927 1108 12-13 0.2319245158515102 1108 14-15 0.18377200694048668 1108 16-17 0.17966838406088925 1108 18-19 0.07465347891701413 1108 20-21 0.19504749565005142 1108 22-23 0.06475880405293566 1108 24-25 0.1871629799105179 1108 26-27 0.023336722537898424 1108 28-29 0.032513120585541344 1108 30-31 0.20518378819893002 1108 32-33 0.15886052926939698 1108 34-35 0.02894530003996465 1108 36-37 0.04508092982214151 1108 38-39 0.051764581603443105 1108 40-41 -0.00736693675223421 1108 42-43 0.007598953198616698 1108 44-45 -0.03443845707231219 1108 46-47 0.029734626213944182 1108 48-49 -0.23570194223466956 1108 50-51 -0.29744977436864417 1108 52-53 -0.09311418872668753 1108 54-55 -0.051415670644622935 1108 56-57 -0.09403895423435671 1108 58-59 -0.034989595820622554 1108 60-61 -0.07509894441837162 1108 62-63 -0.1468519143428786 1108 64-65 -0.22309554175863866 1108 66-67 -0.07230432018052113 1108 68-69 0.014298781064614019 1108 70-71 0.010446760250740539 1108 72-73 -0.23619005438458274 1108 74-75 -0.16084305241180985 1108 76-77 -0.1295660455927603 1109 1 -0.29290029987170385 1109 2 -0.25071281484454744 1109 3 -0.2580277258541628 1109 4 -0.09726973996323096 1109 5 -0.1449596306181462 1109 6 -0.2584968590838983 1109 7 -0.3735807634408417 1109 8 -0.27778580111753826 1109 9 -0.16579981432412438 1109 10-11 -0.23001772231649653 1109 12-13 -0.1661303028929666 1109 14-15 -0.01586199343243777 1109 16-17 -0.06012783504567665 1109 18-19 -0.21885596730178491 1109 20-21 -0.10560961953907366 1109 22-23 -0.16732706300829392 1109 24-25 -0.23011719475881875 1109 26-27 -0.35110164697436375 1109 28-29 -0.10058834281148421 1109 30-31 -0.009283572573842491 1109 32-33 -0.14231385737297586 1109 34-35 0.01189879974224084 1109 36-37 0.0033804299935624726 1109 38-39 -0.08317710506238285 1109 40-41 -0.1397075299302628 1109 42-43 -0.13972958033681238 1109 44-45 -0.1632604014114989 1109 46-47 -0.18597087791520295 1109 48-49 -0.07971572812038374 1109 50-51 -0.1499850059679062 1109 52-53 -0.2141367622123127 1109 54-55 -0.16109105273379498 1109 56-57 -0.29782465949789483 1109 58-59 -0.35003828549985627 1109 60-61 -0.20788252646303818 1109 62-63 -0.39482974384780434 1109 64-65 -0.1493034608237025 1109 66-67 -0.09506907595020664 1109 68-69 -0.27178872201796267 1109 70-71 -0.14245236580109477 1109 72-73 -0.09379093735492461 1109 74-75 -0.1019444797201352 1109 76-77 0.0440606287728329 1110 1 -0.20800670004607724 1110 2 -0.353287530078056 1110 3 -0.3044071320508195 1110 4 -0.2658189309104628 1110 5 -0.3322112023136725 1110 6 -0.35465376658468983 1110 7 -0.38917159115558775 1110 8 -0.45296954316109606 1110 9 -0.24182897793964742 1110 10-11 -0.43046308706986736 1110 12-13 -0.20939203336218526 1110 14-15 -0.2740100744577134 1110 16-17 -0.24171224127906754 1110 18-19 -0.4816701533386123 1110 20-21 -0.6921336927469 1110 22-23 -0.6374348469114892 1110 24-25 -0.57491853783295 1110 26-27 -0.7624640198366208 1110 28-29 -0.6024735202594016 1110 30-31 -0.5021616550440839 1110 32-33 -0.3313893795775371 1110 34-35 -0.37436848768395237 1110 36-37 -0.4481229362635375 1110 38-39 -0.3704728826077641 1110 40-41 -0.31731218513688475 1110 42-43 -0.3505994437027766 1110 44-45 -0.47226847602019717 1110 46-47 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-0.025356261519188905 2313 62-63 -0.1099909562617647 2313 64-65 0.03234730599190172 2313 66-67 0.08274761285549204 2313 68-69 -0.11507455706203729 2313 70-71 -0.3725272359858529 2313 72-73 -0.33044713829684014 2313 74-75 -0.2283181060937629 2313 76-77 -0.08089408684122645 2314 1 -0.3272144413287066 2314 2 -0.22378390398706927 2314 3 -0.37698297427369454 2314 4 -0.2770411027703119 2314 5 -0.49661858941996684 2314 6 -0.2613912860854697 2314 7 -0.19126766148488628 2314 8 -0.2597642473816961 2314 9 -0.19744625668485583 2314 10-11 -0.42022188350280487 2314 12-13 -0.46622125923391877 2314 14-15 -0.3904264371933053 2314 16-17 -0.44619109610893304 2314 18-19 -0.3888833337577182 2314 20-21 -0.4056362700121596 2314 22-23 -0.26138997403549524 2314 24-25 -0.16274188184779348 2314 26-27 -0.04979351977337387 2314 28-29 -0.18985019100190215 2314 30-31 -0.2235706127537398 2314 32-33 -0.2689466960593805 2314 34-35 -0.2666569832778265 2314 36-37 -0.9201353640579484 2314 38-39 -0.4432714026566771 2314 40-41 -0.43024541868243915 2314 42-43 -0.24243599554322515 2314 44-45 -0.2607739520500516 2314 46-47 -0.22466179607041425 2314 48-49 -0.14497889159782318 2314 50-51 -0.028425038425801574 2314 52-53 -0.02462339189537488 2314 54-55 -0.1501623699301149 2314 56-57 -4.219536130456447E-4 2314 58-59 0.04050583495498117 2314 60-61 0.009234634850553647 2314 62-63 0.04361031512797098 2314 64-65 0.1675388426257456 2314 66-67 0.3110523123140396 2314 68-69 0.21626497139287437 2314 70-71 0.25368967989452074 2314 72-73 0.2854804307557295 2314 74-75 0.5116163682157158 2314 76-77 0.5902123534959856 2315 1 -0.3521453722596348 2315 2 -0.36453315323631585 2315 3 -0.149105346646067 2315 4 -0.09272203370124288 2315 5 -0.11024621704759596 2315 6 -0.2630857305299159 2315 7 -0.3377886825059093 2315 8 -0.2548693524868 2315 9 -0.2236955059341028 2315 10-11 -0.4299985351544606 2315 12-13 -0.3156003883630518 2315 14-15 -0.40114753328940367 2315 16-17 -0.4888067117245498 2315 18-19 -0.6567181685925618 2315 20-21 -0.58570346220435 2315 22-23 -0.5138772112727352 2315 24-25 -0.7682546446105576 2315 26-27 -0.6185330092628689 2315 28-29 -0.5256324732841833 2315 30-31 -0.5684407328738601 2315 32-33 -0.499944068431752 2315 34-35 -0.5715451214159657 2315 36-37 -0.7330340127065895 2315 38-39 -0.6708254567107375 2315 40-41 -0.6395201934572157 2315 42-43 -0.8623474069546404 2315 44-45 -0.8684154685665604 2315 46-47 -0.6043036879623074 2315 48-49 -0.3766981108170526 2315 50-51 -0.4665072456330108 2315 52-53 -0.5779571006040811 2315 54-55 -0.5935306131733569 2315 56-57 -0.87721136802746 2315 58-59 -0.6598631590390163 2315 60-61 -0.7386238486329297 2315 62-63 -0.7278634085957556 2315 64-65 -0.45065109731419284 2315 66-67 -0.5822660060042786 2315 68-69 -0.6615173108894083 2315 70-71 -0.5734766113967673 2315 72-73 -0.3566498244995273 2315 74-75 -0.5210116347872891 2315 76-77 -0.3890909498073185 2316 1 -0.9573412786923825 2316 2 -0.6905039134702307 2316 3 -0.841643358341976 2316 4 -1.6182425015374946 2316 5 -1.0971994334218635 2316 6 -1.0908869001205588 2316 7 -0.7766161678860257 2316 8 -0.7150155513172152 2316 9 -0.9328066170452161 2316 10-11 -0.8053347924644001 2316 12-13 -1.3641734877782596 2316 14-15 -2.3506489952777088 2316 16-17 -1.6464192848239634 2316 18-19 -1.3161889288264774 2316 20-21 -1.0144037546020073 2316 22-23 -1.2915731177054752 2316 24-25 -1.0366157557216624 2316 26-27 -0.7779525999061363 2316 28-29 -1.2963283797169325 2316 30-31 -1.3076892708855539 2316 32-33 -1.2240127818820454 2316 34-35 -1.2755919050417006 2316 36-37 -1.1093541881451898 2316 38-39 -1.3865798426756513 2316 40-41 -1.128814053106332 2316 42-43 -1.1805286935043497 2316 44-45 -1.2305982171045784 2316 46-47 -1.135742284453535 2316 48-49 -1.0321440172497987 2316 50-51 -1.214524789492657 2316 52-53 -1.0163182117151948 2316 54-55 -1.1354560517698502 2316 56-57 -1.115768385571318 2316 58-59 -1.095392398805096 2316 60-61 -1.0286165386914128 2316 62-63 -1.0426865080109593 2316 64-65 -0.9529566528697515 2316 66-67 -0.7837645440159733 2316 68-69 -0.9242044453923342 2316 70-71 -0.7951856757242552 2316 72-73 -0.8430387133884132 2316 74-75 -0.970461927184953 2316 76-77 -1.505123113550006 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13440.0 3 1390.0 4 2253.0 5 3092.0 6 3433.0 7 3727.0 8 5140.0 9 5650.0 10 9213.0 11 12189.0 12 7911.0 13 4773.0 14 4081.0 15 3835.0 16 3552.0 17 3727.0 18 3803.0 19 3914.0 20 3953.0 21 4390.0 22 4730.0 23 5319.0 24 5896.0 25 6926.0 26 7916.0 27 9696.0 28 11624.0 29 14120.0 30 17485.0 31 22836.0 32 30346.0 33 43239.0 34 67613.0 35 103583.0 36 164762.0 37 253863.0 38 253721.0 39 73847.0 40 717.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 21.024622359512414 26.449730042807023 31.241803087697807 21.283844509982757 2 20.7598689083407 26.696526803384145 31.232970650847786 21.31063363742737 3 20.825142606041137 26.931277786747582 30.731997183665925 21.511582423545352 4 20.7522451663379 26.797006999758626 31.015922162018196 21.434825671885275 5 20.909975389650004 27.035181065427576 31.005878353128978 21.048965191793442 6 21.103527255688377 26.790863974152934 30.8461135769507 21.259495193207986 7 21.04186416597516 26.59043297397129 30.99815977114911 21.369543088904443 8 20.701686341061876 26.96489404291551 30.75506231080578 21.578357305216826 9 20.783228796292597 26.794025408493464 30.713748285459364 21.70899750975457 10-11 21.118155883448438 26.930044508996474 30.627433272919845 21.324366334635243 12-13 21.24989335913522 26.764339294817773 30.48513790484514 21.50062944120187 14-15 20.861183016682972 27.122966641963792 30.524806416700194 21.491043924653038 16-17 21.088440469280208 26.94108615190648 30.52734728524439 21.44312609356893 18-19 21.08992521801875 26.89920890309635 30.20874122338511 21.802124655499792 20-21 21.158709148008224 27.203840614658137 29.974113218570402 21.663337018763233 22-23 21.250646571185666 27.27150912939788 29.513899147795247 21.963945151621203 24-25 21.24543100172751 27.488941390723376 29.284079639465826 21.981547968083287 26-27 21.384375373217242 27.740148001053644 28.910265986598095 21.96521063913102 28-29 21.45228982380977 27.771494483600602 28.787557246635526 21.988658445954098 30-31 21.35893566087818 27.758560497399532 28.82758500161658 22.054918840105707 32-33 21.35907099411569 27.980197830283405 28.559598963203204 22.101132212397705 34-35 21.25126188964281 27.692579131819926 28.92896050254209 22.127198475995176 36-37 21.324979391863355 27.86914987951366 28.93270033792767 21.873170390695318 38-39 21.283360327323635 27.806195911450832 29.046768128559087 21.863675632666446 40-41 21.136045487760015 27.813647992516362 28.932615596136337 22.11769092358729 42-43 21.119465809751738 28.04274087936151 28.726874595044027 22.110918715842732 44-45 21.340997778986473 27.85255832709449 28.770677128221607 22.03576676569743 46-47 21.23318825122024 27.756598295385736 28.765209589485174 22.245003863908845 48-49 21.137163939733657 27.871560747811337 28.664714160992666 22.32656115146234 50-51 21.046066792854255 28.108742735473864 28.477733218133057 22.36745725353883 52-53 21.0610826752482 27.958011851775392 28.57399118816587 22.406914284810533 54-55 20.984263290346934 28.00608276910822 28.653729545669254 22.355924394875593 56-57 21.032669963333834 28.155002448988427 28.4019819952535 22.410345592424243 58-59 21.192918063349584 28.059250566475864 28.391250158654856 22.356581211519693 60-61 21.117757045127018 27.934729187552254 28.340393337752857 22.60712042956787 62-63 20.98065948847027 27.974115543658485 28.43282426736451 22.612400700506736 64-65 21.11028295169334 27.822161296323344 28.4225560661845 22.644999685798815 66-67 20.929483471401117 27.897654775885734 28.47927901233746 22.693582740375692 68-69 21.077931536780774 27.802143377380084 28.535885963999586 22.58403912183956 70-71 20.874473082344437 27.646119479905316 28.600420118742242 22.878987319008 72-73 20.96264454552078 27.56967848704685 28.53694432590526 22.930732641527108 74-75 20.849759253059926 27.51310326220015 28.49570219661805 23.141435288121876 76-77 20.779355421079764 27.329276853499568 28.81444144571009 23.076926279710577 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 11420.0 1 6738.0 2 1941.0 3 1876.0 4 1926.0 5 1949.0 6 1989.5 7 2042.5 8 2090.5 9 2103.0 10 2146.0 11 2270.5 12 2493.0 13 2634.0 14 2740.0 15 2994.0 16 3418.0 17 3694.0 18 3834.0 19 4248.0 20 4952.5 21 5941.0 22 6499.0 23 7181.0 24 8783.0 25 11054.5 26 12406.0 27 13906.0 28 17666.0 29 20891.0 30 21856.0 31 24830.0 32 29869.0 33 33891.0 34 38039.0 35 40230.0 36 41845.5 37 44898.0 38 46484.0 39 46633.0 40 47526.0 41 49186.0 42 50948.0 43 51943.0 44 51968.0 45 52296.0 46 53075.0 47 53438.5 48 53326.0 49 52791.5 50 51517.0 51 49162.0 52 47547.0 53 45951.5 54 42234.5 55 38227.5 56 34027.0 57 31712.0 58 29622.0 59 24829.0 60 20176.0 61 18226.0 62 16183.0 63 11733.5 64 7957.0 65 6587.0 66 5589.0 67 4008.0 68 2970.5 69 2067.0 70 1618.0 71 1417.5 72 1045.5 73 839.0 74 804.0 75 708.0 76 467.0 77 253.5 78 185.0 79 172.5 80 136.5 81 94.0 82 61.5 83 48.0 84 49.0 85 44.5 86 34.5 87 30.0 88 27.5 89 23.5 90 20.5 91 19.0 92 16.5 93 12.5 94 14.0 95 14.0 96 11.0 97 11.5 98 11.0 99 20.5 100 31.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.002746098252066855 2 0.008571155150390487 3 0.011899759092289705 4 0.01955554815865791 5 0.01481228754145152 6 0.01464585734435656 7 0.018640182074635622 8 0.018973042468825543 9 0.01805767638480326 10-11 0.02184396336871362 12-13 0.02017966139776401 14-15 0.007530966418546981 16-17 0.006449170137429735 18-19 0.012107796838658407 20-21 0.010526709966256277 22-23 0.01452103469653534 24-25 0.02209360866435606 26-27 0.013272808218323134 28-29 0.00882080044603293 30-31 0.007947041911284383 32-33 0.009944204276423915 34-35 0.01115082320536238 36-37 0.009694558980781472 38-39 0.016892665005138533 40-41 0.012565479880669549 42-43 0.015436400780557625 44-45 0.018848219821004323 46-47 0.01676784235731731 48-49 0.014312996950166638 50-51 0.02654561643664627 52-53 0.03045672606837785 54-55 0.023175404945473307 56-57 0.015686046076200066 58-59 0.016434981963127388 60-61 0.018182499032624477 62-63 0.02404916348022185 64-65 0.02042930669340645 66-67 0.02013805384849027 68-69 0.027460982520668552 70-71 0.051260500705247965 72-73 0.061787210671504236 74-75 0.07148176965228571 76-77 0.06890210159731382 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 1201705.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.85381459388844 #Duplication Level Percentage of deduplicated Percentage of total 1 79.31552002056785 50.64598509811186 2 12.055599154000971 15.395919863956326 3 3.9824325235129994 7.6288052396721096 4 1.7009001207750192 4.344358438187621 5 0.8716314530647463 2.7828496599098935 6 0.499766567312946 1.914720103765495 7 0.3320126389287003 1.4840191442286597 8 0.22744973738458513 1.1618826688307133 9 0.15706782155258625 0.9026441600476316 >10 0.7989229900650275 9.103296905378658 >50 0.04319877819908922 1.888547819375087 >100 0.015237722554157581 1.5324091675319431 >500 0.0 0.0 >1k 1.3023604071549116E-4 0.3696492224794995 >5k 0.0 0.0 >10k+ 1.3023604071549116E-4 0.8449125085245703 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10160 0.8454654012424014 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4445 0.3698911130435506 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 8.321509854748046E-5 0.0 2 8.321509854748046E-5 0.0 0.0 1.6643019709496092E-4 0.0 3 2.4964529564244135E-4 0.0 0.0 3.3286039418992184E-4 0.0 4 3.3286039418992184E-4 0.0 0.0 9.985811825697654E-4 0.0 5 4.160754927374023E-4 0.0 0.0 0.0020803774636870115 0.0 6 4.160754927374023E-4 0.0 0.0 0.0037446794346366204 0.0 7 4.160754927374023E-4 0.0 0.0 0.007905434362010643 0.0 8 4.160754927374023E-4 0.0 0.0 0.012565479880669549 0.0 9 4.160754927374023E-4 0.0 0.0 0.016975880103686013 0.0 10 4.992905912848827E-4 0.0 0.0 0.02238486150927224 8.321509854748046E-5 11 6.657207883798437E-4 0.0 0.0 0.02929171468871312 8.321509854748046E-5 12 7.489358869273241E-4 0.0 0.0 0.03936074161295826 8.321509854748046E-5 13 7.489358869273241E-4 0.0 0.0 0.05234229698636521 8.321509854748046E-5 14 8.321509854748045E-4 0.0 0.0 0.06773709021764909 8.321509854748046E-5 15 8.321509854748045E-4 0.0 0.0 0.08612762699664227 8.321509854748046E-5 16 8.321509854748045E-4 0.0 0.0 0.10876213380155696 8.321509854748046E-5 17 8.321509854748045E-4 0.0 0.0 0.12548836860960053 8.321509854748046E-5 18 9.15366084022285E-4 0.0 0.0 0.14570963755663827 8.321509854748046E-5 19 0.0011650113796647264 0.0 0.0 0.1664301970949609 8.321509854748046E-5 20 0.0012482264782122069 0.0 0.0 0.1907290058708252 8.321509854748046E-5 21 0.0012482264782122069 0.0 0.0 0.21477816935104704 1.6643019709496092E-4 22 0.0015810868724021286 0.0 0.0 0.24215593677316813 1.6643019709496092E-4 23 0.00183073216804457 0.0 0.0 0.2741937497139481 1.6643019709496092E-4 24 0.00183073216804457 0.0 0.0 0.30265331341718643 1.6643019709496092E-4 25 0.00183073216804457 0.0 0.0 0.3358561377376311 1.6643019709496092E-4 26 0.00183073216804457 0.0 0.0 0.3726372112956175 1.6643019709496092E-4 27 0.0019139472665920506 0.0 0.0 0.4078371979812017 1.6643019709496092E-4 28 0.0019139472665920506 0.0 0.0 0.44295396956823846 1.6643019709496092E-4 29 0.0019139472665920506 0.0 0.0 0.47615679388868315 1.6643019709496092E-4 30 0.0019139472665920506 0.0 0.0 0.5151846751074515 1.6643019709496092E-4 31 0.0019139472665920506 0.0 0.0 0.5568754394797392 1.6643019709496092E-4 32 0.0020803774636870115 0.0 0.0 0.6048905513416354 1.6643019709496092E-4 33 0.0020803774636870115 0.0 0.0 0.6552356859628611 1.6643019709496092E-4 34 0.0020803774636870115 0.0 0.0 0.7106569415954831 1.6643019709496092E-4 35 0.0020803774636870115 0.0 0.0 0.7645803254542504 1.6643019709496092E-4 36 0.0020803774636870115 0.0 0.0 0.823746260521509 1.6643019709496092E-4 37 0.0020803774636870115 0.0 0.0 0.8890701128812811 1.6643019709496092E-4 38 0.0020803774636870115 0.0 0.0 0.9532289538613886 1.6643019709496092E-4 39 0.0020803774636870115 0.0 0.0 1.0145584814908817 1.6643019709496092E-4 40 0.0020803774636870115 0.0 0.0 1.0886199191981394 1.6643019709496092E-4 41 0.0020803774636870115 0.0 0.0 1.164595304171989 1.6643019709496092E-4 42 0.0020803774636870115 0.0 0.0 1.2446482289746652 1.6643019709496092E-4 43 0.0020803774636870115 0.0 0.0 1.3300269200843802 1.6643019709496092E-4 44 0.0020803774636870115 0.0 0.0 1.4257242834139827 1.6643019709496092E-4 45 0.0020803774636870115 0.0 0.0 1.5294935113026908 1.6643019709496092E-4 46 0.002163592562234492 0.0 0.0 1.6397535168781023 1.6643019709496092E-4 47 0.002246807660781972 0.0 0.0 1.7539246320852455 1.6643019709496092E-4 48 0.002246807660781972 0.0 0.0 1.8622706903940651 3.3286039418992184E-4 49 0.002330022759329453 0.0 0.0 1.977273956586683 3.3286039418992184E-4 50 0.002330022759329453 0.0 0.0 2.098019064579077 3.3286039418992184E-4 51 0.002330022759329453 0.0 0.0 2.229914995776834 3.3286039418992184E-4 52 0.002330022759329453 0.0 0.0 2.368468134858389 3.3286039418992184E-4 53 0.002330022759329453 0.0 0.0 2.517672806554021 3.3286039418992184E-4 54 0.002330022759329453 0.0 0.0 2.6723696747537873 3.3286039418992184E-4 55 0.002330022759329453 0.0 0.0 2.8331412451475195 3.3286039418992184E-4 56 0.0024964529564244138 0.0 0.0 3.01313550330572 3.3286039418992184E-4 57 0.0024964529564244138 0.0 0.0 3.1918815349857077 4.160754927374023E-4 58 0.0024964529564244138 0.0 0.0 3.376369408465472 4.160754927374023E-4 59 0.0024964529564244138 0.0 0.0 3.558360828988812 4.160754927374023E-4 60 0.0024964529564244138 0.0 0.0 3.7525016539000835 4.160754927374023E-4 61 0.002746098252066855 0.0 0.0 3.957626871819623 4.160754927374023E-4 62 0.002746098252066855 0.0 0.0 4.164998897399944 4.160754927374023E-4 63 0.002746098252066855 0.0 0.0 4.391427180547638 4.160754927374023E-4 64 0.002746098252066855 0.0 0.0 4.622432294115444 4.160754927374023E-4 65 0.002746098252066855 0.0 0.0 4.8749069031085 4.160754927374023E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTTAC 110 2.780871E-8 29.039667 4 CGATTGC 105 1.8220444E-5 23.677715 68 GTCGGGT 150 5.548991E-7 21.309055 66 CTCGCGC 90 0.004868242 19.717472 36 CGTAGAG 115 9.1665785E-4 18.521132 15 GTACGAA 100 0.008923703 17.752373 61 TCGCGCT 100 0.008938692 17.747202 37 GCGGTTA 165 3.0129586E-5 17.207975 3 CAGCGTA 125 0.001604295 17.038023 12 TTAGGCG 150 2.8397815E-4 16.563364 6 TCGGGTG 215 9.421801E-7 16.51865 67 GGCGGTT 180 6.3482206E-5 15.773977 2 TAGGCGT 160 4.6419018E-4 15.528154 7 CGGGTGC 230 1.9027939E-6 15.441989 68 TTGCGCG 140 0.0034204444 15.211254 5 TCGCCCG 165 5.858524E-4 15.059484 58 ATCGATT 165 5.858524E-4 15.059484 23 CGCTCCG 145 0.004315996 14.688561 25 AACTCGA 145 0.00431719 14.68795 13 TACGTAG 145 0.004320773 14.686116 2 >>END_MODULE