FastQCFastQC Report
Thu 26 May 2016
SRR936000_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936000_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1201705
Sequences flagged as poor quality0
Sequence length125
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92510.7698228766627416No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72170.6005633662171664No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54370.45244049080265125No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14770.12290870055462863No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14240.11849830033161216No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA7750.051.45409100-101
CGGTATC7200.045.46498100-101
GGTATCA64850.044.3230631
TACGCAG6450.043.830936100-101
CGTACAT8750.041.1523100-101
GCGTATC1800.039.678528100-101
AGTATCA22500.039.414005100-101
CCGTATC3650.039.134987100-101
TCGTATC2900.038.994415100-101
AGGTATC15300.038.900517100-101
TCGGTAT2300.037.522087100-101
CCTGTCG2250.037.03021296-97
CCGTACA3550.036.884266100-101
TCGAGTA2200.036.52228100-101
CGAGTAC6550.034.075073100-101
TCCGTAC1700.033.2585698-99
ACGTATC2250.033.06544100-101
AAGTATC14600.032.81632100-101
CCGGTAT2600.032.048042100-101
TGTATCA15950.031.904522100-101
Produced by FastQC (version 0.11.5)