FastQCFastQC Report
Thu 26 May 2016
SRR935999_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935999_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2406064
Sequences flagged as poor quality0
Sequence length77
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71710.2980386224140339No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37200.1546093536996522No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAA2300.026.23815359
CGATTGC1451.479566E-824.49763768
ACGTCGC750.001682398123.68104768
GACGTAT5400.021.69093139
TCGCGGA2750.020.65377658
TAACGCG1054.9652497E-420.28875552
ATCCGCG900.004868579619.7182082
ATACGTA2900.019.5830361
ATTCGCG2554.5474735E-1119.4886656
CGATCGT2809.094947E-1219.0147781
TCGCGCT3002.3646862E-1117.74749637
CTCGCGC3002.3646862E-1117.74565136
TGAGACG6850.017.61612136
GCGTAAG5650.017.58970638
ACGTATT6900.017.49210440
TGCGTAA6000.017.15591237
GTTACGC1250.001604526217.03865855
CGGTTAC1905.3664626E-616.811674
TTGCGTA6000.016.56260736
CGTATTA7500.016.09273541
Produced by FastQC (version 0.11.5)