FastQCFastQC Report
Thu 26 May 2016
SRR935999_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935999_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2406064
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63300.26308527121473074No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46480.1931785688161246No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35530.14766855744485602No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA10250.046.456978100-101
GGTATCA59700.037.5741651
CGGTATC8900.036.115086100-101
TCGTATC4650.035.84181100-101
CGAGCGT1900.032.89292596-97
CCTGTCG6250.031.90300496-97
ACGTATC4050.031.598633100-101
GTATCAA117700.031.1911261
CGTACAT12200.030.493345100-101
CGAGTAC7900.028.631319100-101
CCGTATC5250.028.34429100-101
CGCATAA1204.3473847E-1027.28138100-101
CACGTAT4500.027.11575598-99
CGATACG557.7392324E-427.05478796-97
GCGTACA4200.026.927073100-101
AGGTATC16300.026.292372100-101
CCGTACG2050.026.13096496-97
TCGGTAT2750.025.973675100-101
CGCTTAA1850.025.73968100-101
TACGCAG7500.025.396482100-101
Produced by FastQC (version 0.11.5)