FastQCFastQC Report
Thu 26 May 2016
SRR935998_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935998_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2414974
Sequences flagged as poor quality0
Sequence length77
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69210.2865869363396873No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35620.14749641196965269No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGG851.1898794E-425.05720911
CGGTTAC1505.582224E-721.2977434
GGCGATA850.003489874320.88533259
CTCGCGC2103.6436177E-818.59211336
GCGTTCG1159.1709086E-418.5209310
TCGCGCT2356.675691E-918.1257437
CGCGCTT2752.5556801E-916.78073738
CGCGGAA1956.8448226E-616.38695359
GACGTAT6300.016.34123239
AATTCGC2652.9380317E-816.076155
TGCGTAA7100.015.99830437
ACTCGAG3551.8189894E-1115.9966514
ATACGTA2251.524737E-615.7754531
TTGCGTA7250.015.66633236
GCGTAAG7350.015.45478638
ATTCGCG2553.326095E-715.31368956
CGTAAGT7600.014.94733339
CCTCGCG2908.911593E-814.68688335
ACGTATT7250.014.19908340
CGATCGT3051.6500417E-713.9651571
Produced by FastQC (version 0.11.5)