##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935998_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2414974 Sequences flagged as poor quality 0 Sequence length 77 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.23984978720268 40.0 37.0 41.0 31.0 41.0 2 37.11273537520487 40.0 37.0 41.0 31.0 41.0 3 36.985781213379525 40.0 36.0 41.0 31.0 41.0 4 36.88584224923333 40.0 36.0 41.0 31.0 41.0 5 36.814998422343265 39.0 36.0 41.0 30.0 41.0 6 36.72211295028435 39.0 36.0 41.0 30.0 41.0 7 36.58736822839501 39.0 36.0 41.0 30.0 41.0 8 36.46235446013083 39.0 36.0 41.0 30.0 41.0 9 36.62392597187382 39.0 36.0 41.0 30.0 41.0 10-11 36.83849867534806 39.5 36.0 41.0 30.5 41.0 12-13 36.76897598069379 40.0 36.0 41.0 30.0 41.0 14-15 36.625106108802825 40.0 36.0 41.0 30.0 41.0 16-17 36.47939708667671 40.0 36.0 41.0 30.0 41.0 18-19 36.33529098035838 40.0 36.0 41.0 30.0 41.0 20-21 36.13917830999423 39.5 35.0 41.0 29.5 41.0 22-23 35.88421780110262 39.0 35.0 41.0 28.5 41.0 24-25 35.66813845614901 39.0 35.0 41.0 27.5 41.0 26-27 34.71846694829841 38.0 34.0 40.0 25.5 40.5 28-29 34.74126885009942 38.0 34.5 40.0 26.0 40.5 30-31 35.159774183904254 39.0 35.0 40.0 26.0 41.0 32-33 35.0543386802508 39.0 34.5 41.0 26.0 41.0 34-35 34.8217148507603 38.0 34.0 40.0 26.0 41.0 36-37 34.53439395206739 38.0 34.0 40.0 25.5 41.0 38-39 34.24283781109031 38.0 34.0 40.0 24.5 41.0 40-41 33.90091715273125 37.0 33.5 40.0 23.0 41.0 42-43 33.544399442809734 37.0 33.0 39.5 22.5 41.0 44-45 33.19277453918758 36.0 33.0 39.0 22.0 41.0 46-47 32.78161752466072 36.0 32.5 39.0 21.5 40.5 48-49 32.36633603508775 35.0 32.0 38.5 20.0 40.0 50-51 31.91135494626443 35.0 32.0 37.5 18.5 39.5 52-53 31.424212227543652 35.0 31.0 37.0 15.5 39.0 54-55 30.958780922693165 35.0 31.0 36.0 10.0 39.0 56-57 30.517752365035815 35.0 31.0 36.0 7.0 37.5 58-59 30.087633448641682 34.0 30.0 35.5 4.0 37.0 60-61 29.722520408087206 34.0 30.0 35.0 2.0 37.0 62-63 29.353397593514465 34.0 29.5 35.0 2.0 36.0 64-65 28.97911551014628 34.0 29.0 35.0 2.0 36.0 66-67 28.63204448577914 34.0 29.0 35.0 2.0 35.5 68-69 28.283282759980025 34.0 29.0 35.0 2.0 35.0 70-71 27.84303661240245 34.0 28.5 35.0 2.0 35.0 72-73 27.392927211638717 34.0 27.0 35.0 2.0 35.0 74-75 26.844043041457176 33.5 26.5 35.0 2.0 35.0 76-77 25.06270150320459 31.5 21.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.9957270231159328 1101 2 1.0480222471195617 1101 3 1.0161466362233753 1101 4 0.9365545195931588 1101 5 0.9089679187346391 1101 6 0.9011948331792823 1101 7 1.0704409668791826 1101 8 1.0886097092999165 1101 9 1.0673424708254515 1101 10-11 1.0758359979598708 1101 12-13 1.1986422995371697 1101 14-15 1.1882162362854203 1101 16-17 1.1193295069900344 1101 18-19 1.0990834995892769 1101 20-21 1.149683048341366 1101 22-23 1.0749135963588614 1101 24-25 1.1142385785954474 1101 26-27 1.064502181709429 1101 28-29 1.1623418558187382 1101 30-31 1.315609358494477 1101 32-33 1.35484662949613 1101 34-35 1.2890058968425677 1101 36-37 1.304278994226948 1101 38-39 1.2587218624526244 1101 40-41 1.2674222025055784 1101 42-43 1.3211464836414422 1101 44-45 1.2812929935838469 1101 46-47 1.1761936196857974 1101 48-49 1.085283270740426 1101 50-51 1.0719505245974439 1101 52-53 1.2074367392905678 1101 54-55 1.1411662327900451 1101 56-57 1.3035557064011698 1101 58-59 1.1980266364066487 1101 60-61 1.0887631415214543 1101 62-63 1.1426445383558708 1101 64-65 1.211018908419426 1101 66-67 1.150282053966933 1101 68-69 0.9391390759452882 1101 70-71 0.8365959901600668 1101 72-73 0.9025960823648198 1101 74-75 1.1831669811328673 1101 76-77 1.3450699432279265 1102 1 0.5990830187368701 1102 2 0.643344464626388 1102 3 0.6466791971414096 1102 4 0.5644328261197771 1102 5 0.695279987922504 1102 6 0.645619450252525 1102 7 0.66709712901595 1102 8 0.6290885090678984 1102 9 0.7504669918296827 1102 10-11 0.7129346002154122 1102 12-13 0.8426080464751422 1102 14-15 0.8936252454111298 1102 16-17 0.8871396278120187 1102 18-19 0.8594103501107853 1102 20-21 1.055025814731188 1102 22-23 0.8968121040793235 1102 24-25 1.0334957282375612 1102 26-27 0.8542510899257465 1102 28-29 1.0383216489437004 1102 30-31 1.1545173646051765 1102 32-33 1.0816268746169797 1102 34-35 1.0999877039512214 1102 36-37 1.1013813574337092 1102 38-39 1.2351852389119102 1102 40-41 1.2184571174268441 1102 42-43 1.2493390170738579 1102 44-45 1.1353638255892022 1102 46-47 1.1596246979823164 1102 48-49 1.1440748350745693 1102 50-51 1.2116780999258303 1102 52-53 1.0914838295812395 1102 54-55 1.0892784568472926 1102 56-57 1.1574416155031848 1102 58-59 1.2155181842085092 1102 60-61 1.1086245998956201 1102 62-63 1.0352506332556892 1102 64-65 0.9934274283942273 1102 66-67 0.9948411449302554 1102 68-69 0.9726177910634561 1102 70-71 1.018528312354281 1102 72-73 1.1540886743703531 1102 74-75 1.0396648197221587 1102 76-77 1.0861653799215176 1103 1 0.510716396792354 1103 2 0.5502338342226167 1103 3 0.43706307841413405 1103 4 0.3811412012173463 1103 5 0.45181548696890417 1103 6 0.44931617154570347 1103 7 0.6086322150707488 1103 8 0.45390268145133916 1103 9 0.3919515887312741 1103 10-11 0.6162328962344006 1103 12-13 0.5655413769807893 1103 14-15 0.5288652977085135 1103 16-17 0.4372402526571477 1103 18-19 0.38425384117714856 1103 20-21 0.3787187707315809 1103 22-23 0.13448188641415015 1103 24-25 0.2577542594906035 1103 26-27 0.20110227082127352 1103 28-29 0.18067650472033847 1103 30-31 0.2676484139089297 1103 32-33 0.27394087771106257 1103 34-35 0.31501475676131463 1103 36-37 0.3728763609794754 1103 38-39 0.3548485316976553 1103 40-41 0.45882343725498487 1103 42-43 0.3496932782914186 1103 44-45 0.32144788761086573 1103 46-47 0.3113516725844576 1103 48-49 0.2520342807877469 1103 50-51 0.23091904368977012 1103 52-53 0.3128345837187787 1103 54-55 0.30556420387468464 1103 56-57 0.3172183916833191 1103 58-59 0.41489806429956033 1103 60-61 0.35710108876754276 1103 62-63 0.2613266065950377 1103 64-65 0.3320479843627737 1103 66-67 0.38859347498421215 1103 68-69 0.29113114408644236 1103 70-71 0.15626305715151645 1103 72-73 0.2319749833639726 1103 74-75 0.3763362227699609 1103 76-77 0.39898995969326023 1104 1 0.22592209693781484 1104 2 0.12480629433724744 1104 3 0.2515033793002175 1104 4 0.20144333326646802 1104 5 0.13750050699127314 1104 6 0.14725873545211954 1104 7 0.14207551206826707 1104 8 0.19075412764013322 1104 9 0.1376740166826167 1104 10-11 0.1644463126707123 1104 12-13 0.2100175500714201 1104 14-15 0.20395454358089893 1104 16-17 0.277801270175992 1104 18-19 0.14667256368623072 1104 20-21 0.18936286224916898 1104 22-23 0.2578929187107235 1104 24-25 0.21581666785422016 1104 26-27 0.0971985607428607 1104 28-29 0.16347313134226482 1104 30-31 0.3011826228949417 1104 32-33 0.27062204683199553 1104 34-35 0.3045921138085461 1104 36-37 0.25380049039758035 1104 38-39 0.2791912851066769 1104 40-41 0.3489481496688498 1104 42-43 0.37822515471485474 1104 44-45 0.31096222200471857 1104 46-47 0.15142335904167936 1104 48-49 0.1562441940338104 1104 50-51 0.11220247467199229 1104 52-53 0.1695717778053698 1104 54-55 0.161031165516345 1104 56-57 0.24241013991723293 1104 58-59 0.31337581368092415 1104 60-61 0.36713545422359317 1104 62-63 0.20887075142833922 1104 64-65 0.0883774146379892 1104 66-67 0.0246684386194147 1104 68-69 -0.12266545645714189 1104 70-71 -0.026236783480857895 1104 72-73 0.07272543104213014 1104 74-75 -0.04360218888531975 1104 76-77 0.1177031964787858 1105 1 -0.0847563761409944 1105 2 0.16941025816068134 1105 3 0.13115826498741967 1105 4 0.19992355441769405 1105 5 0.04946283555032949 1105 6 0.2684547846276075 1105 7 0.14578815253079824 1105 8 0.10193669749874346 1105 9 0.10604301227570545 1105 10-11 0.2812620254001885 1105 12-13 0.38082550371613166 1105 14-15 0.31125587554651446 1105 16-17 0.3695670359608485 1105 18-19 0.2593660639426716 1105 20-21 0.12836233028241395 1105 22-23 0.27634211553277055 1105 24-25 0.2360623121556742 1105 26-27 0.15918566849686044 1105 28-29 0.2419911235457377 1105 30-31 0.4095304917075637 1105 32-33 0.4849245522329042 1105 34-35 0.287398566925539 1105 36-37 0.24853380762355926 1105 38-39 0.23859287902882897 1105 40-41 0.4816392373536402 1105 42-43 0.4820911330038271 1105 44-45 0.16535486857109305 1105 46-47 0.24833809317840405 1105 48-49 0.1272260435825885 1105 50-51 0.15663353163977334 1105 52-53 -0.005668702250471114 1105 54-55 0.08728957066165677 1105 56-57 0.08413775669205847 1105 58-59 0.17922393682027504 1105 60-61 0.1747046063689126 1105 62-63 0.06941457263099338 1105 64-65 0.17907165244196221 1105 66-67 0.054205927792867925 1105 68-69 0.03465770521977163 1105 70-71 -0.06050925360099768 1105 72-73 -0.3417704509841748 1105 74-75 -0.45380392296976524 1105 76-77 -0.6093939747014687 1106 1 -0.02472639478210681 1106 2 -0.09294090555280832 1106 3 -0.23569427754028993 1106 4 -0.022990632238922615 1106 5 -0.07028311150663313 1106 6 -0.10434746258265193 1106 7 -0.45883151741019645 1106 8 -0.27397822994937115 1106 9 -0.23629097335379612 1106 10-11 -0.3936151694979131 1106 12-13 -0.04364793127263056 1106 14-15 -0.18831208529287835 1106 16-17 -0.1345084769551832 1106 18-19 -0.20054965853599072 1106 20-21 -0.17751381266322142 1106 22-23 -0.16512982574059265 1106 24-25 -0.35988722481395996 1106 26-27 -0.28318692678548274 1106 28-29 -0.1418354820020653 1106 30-31 0.03984690173779626 1106 32-33 -0.04827912474774365 1106 34-35 -0.14722194514836673 1106 36-37 -0.0867478501507577 1106 38-39 -0.032950111856152375 1106 40-41 -0.08933347611277753 1106 42-43 -0.03196088939976249 1106 44-45 -0.10615538261289004 1106 46-47 0.008126014851477237 1106 48-49 -0.0741915408662166 1106 50-51 -0.18013845159750375 1106 52-53 -0.09932681698608192 1106 54-55 -0.17655816314441353 1106 56-57 -0.1118832188404113 1106 58-59 -0.24627468793386242 1106 60-61 -0.39331127497313645 1106 62-63 -0.3308354088135381 1106 64-65 -0.34666175254403697 1106 66-67 -0.35104876745493385 1106 68-69 -0.5028662741579595 1106 70-71 -0.6419066625435939 1106 72-73 -0.5564747338723173 1106 74-75 -0.7092510241770107 1106 76-77 -0.5880705271520767 1107 1 -0.4252770994579791 1107 2 -0.21415349679116957 1107 3 -0.19607314939710108 1107 4 -0.25258229663066345 1107 5 -0.15367056052758699 1107 6 -0.422043085623109 1107 7 -0.23975914991434877 1107 8 -0.43854265726062636 1107 9 -0.3576226535735145 1107 10-11 -0.24644385163960436 1107 12-13 -0.17447389866810425 1107 14-15 -0.3768772509213463 1107 16-17 -0.3426642108742044 1107 18-19 -0.26754229466624935 1107 20-21 -0.3993017019170182 1107 22-23 -0.4214594654197299 1107 24-25 -0.2610014047786038 1107 26-27 -0.6150841262814097 1107 28-29 -0.5911239985842087 1107 30-31 -0.3579140013668578 1107 32-33 -0.2061200225580322 1107 34-35 -0.2483624418821222 1107 36-37 -0.31740200885518277 1107 38-39 -0.30844471890043224 1107 40-41 -0.3386735749411187 1107 42-43 -0.18757778755747978 1107 44-45 -0.22400745858717386 1107 46-47 -0.23089461873636452 1107 48-49 -0.38528530853136544 1107 50-51 -0.31020137056322383 1107 52-53 -0.34330817016780557 1107 54-55 -0.39893675787375216 1107 56-57 -0.41025307853945847 1107 58-59 -0.5183594896291659 1107 60-61 -0.5999562505555858 1107 62-63 -0.6081997645679991 1107 64-65 -0.6213441934166184 1107 66-67 -0.5271994668915809 1107 68-69 -0.6663564878356496 1107 70-71 -0.7731872706522616 1107 72-73 -0.7254780263121141 1107 74-75 -0.760318654309728 1107 76-77 -0.685597681507943 1108 1 -0.030551190508923298 1108 2 0.1811812533670718 1108 3 0.31545782046705284 1108 4 0.23733693000077238 1108 5 0.17420233351610648 1108 6 0.31126978994989685 1108 7 0.31143503651628635 1108 8 0.16657800058789718 1108 9 0.13908562539511138 1108 10-11 0.1515686421602922 1108 12-13 0.2279401387904656 1108 14-15 0.17110763794637762 1108 16-17 0.13555096476196837 1108 18-19 0.020069140069530533 1108 20-21 0.16134485027097867 1108 22-23 0.2662303490614306 1108 24-25 0.1270045049270081 1108 26-27 0.056610154557112935 1108 28-29 0.20050458153743023 1108 30-31 0.2742447336782945 1108 32-33 0.27045630385651975 1108 34-35 0.2700877784192599 1108 36-37 0.22233539698558502 1108 38-39 0.259736039434614 1108 40-41 0.2334048106741804 1108 42-43 0.13729818591178145 1108 44-45 0.068716758520317 1108 46-47 0.15405289928184374 1108 48-49 0.09576104394889029 1108 50-51 -0.013862749844555822 1108 52-53 0.10464895071440239 1108 54-55 0.14014941288680305 1108 56-57 0.13032878060136355 1108 58-59 0.2278263664058109 1108 60-61 0.14294293910187506 1108 62-63 0.0949960158721872 1108 64-65 0.1849571500451539 1108 66-67 0.18215964865487422 1108 68-69 0.20083875974619048 1108 70-71 0.26554906914570253 1108 72-73 0.33218954116995647 1108 74-75 0.4790859759488626 1108 76-77 0.5559872166212152 1109 1 -0.04566176893176532 1109 2 -0.19358716405621124 1109 3 -0.30108543400287857 1109 4 -0.08654505400762957 1109 5 -0.07344637681275401 1109 6 -0.28965358503162975 1109 7 -0.18179070108366346 1109 8 -0.09625714151399478 1109 9 -0.23001546314971932 1109 10-11 -0.27284135997410175 1109 12-13 -0.2628911285515443 1109 14-15 -0.08781038461260238 1109 16-17 -0.2645509939619828 1109 18-19 -0.264138093910141 1109 20-21 -0.274103948717638 1109 22-23 -0.2512692815229016 1109 24-25 -0.3240283812765412 1109 26-27 -0.5112311444725535 1109 28-29 -0.250432420777571 1109 30-31 -0.17798473091527 1109 32-33 -0.2024372880130514 1109 34-35 -0.07715391793748694 1109 36-37 -0.13361859845007729 1109 38-39 -0.09758446559764877 1109 40-41 -0.18145932645291651 1109 42-43 -0.060787420011997995 1109 44-45 -0.09487987240880358 1109 46-47 -0.12154235249546019 1109 48-49 0.0225346496099732 1109 50-51 -0.17301260125736562 1109 52-53 -0.13688634079560558 1109 54-55 -0.1012860543008749 1109 56-57 -0.08374546373836722 1109 58-59 -0.04555189881821775 1109 60-61 -0.025496649122793258 1109 62-63 -0.22130309588836639 1109 64-65 -5.903239726094966E-4 1109 66-67 -0.01380386949574941 1109 68-69 -0.12822341701510354 1109 70-71 -0.12478931560481854 1109 72-73 -0.12657677468864748 1109 74-75 -0.19656394934707677 1109 76-77 -0.08715215980513946 1110 1 -0.3716769575140333 1110 2 -0.38677509287865774 1110 3 -0.5927396825824331 1110 4 -0.3465570072465596 1110 5 -0.2660721846524865 1110 6 -0.3555089189652847 1110 7 -0.35897117108501675 1110 8 -0.7225391263891652 1110 9 -0.4593966838144752 1110 10-11 -0.47061303717290315 1110 12-13 -0.4270639245735097 1110 14-15 -0.43166052287173784 1110 16-17 -0.4542009234378739 1110 18-19 -0.3961682320098916 1110 20-21 -0.7036415177475988 1110 22-23 -0.5979713829023083 1110 24-25 -0.655835392249621 1110 26-27 -0.6528529599304846 1110 28-29 -0.5496941887412987 1110 30-31 -0.3942098183731346 1110 32-33 -0.4274405950758222 1110 34-35 -0.4202007188523069 1110 36-37 -0.4184728087792706 1110 38-39 -0.49062576802256785 1110 40-41 -0.4749471221959638 1110 42-43 -0.5633804759770698 1110 44-45 -0.5683946027835773 1110 46-47 -0.5429011170086824 1110 48-49 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-0.4125836680928998 2314 6 -0.39315888157840106 2314 7 -0.3693288541748103 2314 8 -0.4386175460863768 2314 9 -0.25249107509537794 2314 10-11 -0.4619995980493954 2314 12-13 -0.4187295988370181 2314 14-15 -0.36485179941899304 2314 16-17 -0.4995556321809076 2314 18-19 -0.4937436956816441 2314 20-21 -0.3872093110971946 2314 22-23 -0.20566062750410907 2314 24-25 -0.20556108820095886 2314 26-27 -0.1842856360639118 2314 28-29 -0.2954372086164625 2314 30-31 -0.38605942120194214 2314 32-33 -0.6370093749123313 2314 34-35 -0.6288348982474972 2314 36-37 -1.1586302688073644 2314 38-39 -0.6886864166419926 2314 40-41 -0.6033503731941892 2314 42-43 -0.32053665695018196 2314 44-45 -0.3641151647662184 2314 46-47 -0.24629712537235804 2314 48-49 -0.15041106826661377 2314 50-51 -0.20820591025252 2314 52-53 -0.10281607925990599 2314 54-55 -0.11481873130129472 2314 56-57 -0.2528719823810164 2314 58-59 -0.2868672079330672 2314 60-61 -0.1901579095213819 2314 62-63 -0.3423742252198636 2314 64-65 -0.2649542895001673 2314 66-67 -0.17288505328891546 2314 68-69 -0.12862559548179675 2314 70-71 0.028314101715192663 2314 72-73 0.08056593283424363 2314 74-75 0.19407702258421367 2314 76-77 -0.03367618877156531 2315 1 -0.9879857391102433 2315 2 -0.7661649184051811 2315 3 -0.68652108427775 2315 4 -0.533855845482897 2315 5 -0.748167364838956 2315 6 -0.6783289370912726 2315 7 -1.2785374440612998 2315 8 -1.0350627744682797 2315 9 -0.7555158773375297 2315 10-11 -0.7822750522702151 2315 12-13 -0.8091016865754952 2315 14-15 -0.8465203778349846 2315 16-17 -0.7522518750524299 2315 18-19 -0.8030250582899896 2315 20-21 -0.8214523936306648 2315 22-23 -0.7679045703247738 2315 24-25 -0.8795468691144848 2315 26-27 -0.8647991842345846 2315 28-29 -0.7793208912949297 2315 30-31 -1.003722361760957 2315 32-33 -0.9561529292510969 2315 34-35 -0.9660310924235134 2315 36-37 -0.9930033412770953 2315 38-39 -0.8365791188094889 2315 40-41 -0.8836161153677367 2315 42-43 -0.9804613412410177 2315 44-45 -0.9269184295290742 2315 46-47 -0.8785150160996444 2315 48-49 -0.7943717439437563 2315 50-51 -0.7072674397241165 2315 52-53 -0.7350541245142601 2315 54-55 -0.8469581681648712 2315 56-57 -0.8907459908864972 2315 58-59 -0.7512225065447034 2315 60-61 -0.8326244448905484 2315 62-63 -0.887794548557995 2315 64-65 -0.597018518866804 2315 66-67 -0.6332895235359643 2315 68-69 -0.70400623198611 2315 70-71 -0.7089311937582927 2315 72-73 -0.548038481142445 2315 74-75 -0.6642444975811337 2315 76-77 -0.8641963649410691 2316 1 -1.520203908554187 2316 2 -1.0402565753365707 2316 3 -1.416876764944476 2316 4 -2.126142772057385 2316 5 -1.541148547018011 2316 6 -1.4866917547663832 2316 7 -1.2893834788287677 2316 8 -1.1926224888056467 2316 9 -1.3727044003960884 2316 10-11 -1.3219556339257608 2316 12-13 -1.8485383672741236 2316 14-15 -3.019355994210912 2316 16-17 -2.1613903752476418 2316 18-19 -1.6570046468713855 2316 20-21 -1.3167717132297838 2316 22-23 -1.75542148995671 2316 24-25 -1.7289575114300462 2316 26-27 -1.6433525348154419 2316 28-29 -2.1643958389978906 2316 30-31 -2.172690959227914 2316 32-33 -2.1814339356634704 2316 34-35 -2.207289776315413 2316 36-37 -2.2896434175996347 2316 38-39 -2.335408379024692 2316 40-41 -1.9985639550478638 2316 42-43 -2.145290154823506 2316 44-45 -1.9711975810484574 2316 46-47 -1.8838213620820028 2316 48-49 -1.7170113128672355 2316 50-51 -1.9112287983990477 2316 52-53 -1.9516831413602098 2316 54-55 -1.9334515545067212 2316 56-57 -1.7666849450063822 2316 58-59 -2.147028108898791 2316 60-61 -1.7828277083476358 2316 62-63 -1.7563222101623204 2316 64-65 -1.9119700804861672 2316 66-67 -1.7970362402538527 2316 68-69 -1.9865272457512084 2316 70-71 -1.7931943029599893 2316 72-73 -1.8249935246739426 2316 74-75 -1.7933856866875466 2316 76-77 -2.17146022308809 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 43713.0 3 4251.0 4 5491.0 5 6898.0 6 7922.0 7 8502.0 8 9772.0 9 10097.0 10 12844.0 11 14363.0 12 11128.0 13 9255.0 14 8921.0 15 9234.0 16 9508.0 17 10231.0 18 10664.0 19 11387.0 20 12335.0 21 13678.0 22 15045.0 23 17141.0 24 20482.0 25 23746.0 26 28272.0 27 33192.0 28 40013.0 29 48148.0 30 58924.0 31 71663.0 32 89367.0 33 114355.0 34 154141.0 35 210583.0 36 303830.0 37 425946.0 38 413685.0 39 115377.0 40 870.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.30447988637234 27.183802261372602 28.869748499423377 21.641969352831687 2 22.15237418449655 27.19973695173777 28.839191872007476 21.808696991758207 3 22.060582367304207 27.553323482541902 28.39065455858626 21.995439591567635 4 21.81730303694221 27.304606624722872 28.94776187709179 21.930328461243132 5 22.084877580492396 27.247498247126757 28.8675548443287 21.800069328052142 6 22.29766051113421 27.112311202958633 28.82420909207622 21.765819193830936 7 22.161814940841637 26.999390359058633 28.79555326572062 22.04324143437911 8 22.016442327604057 27.343342306895842 28.601076827500517 22.039138537999587 9 22.088545523521745 27.150313186986132 28.59763299885858 22.163508290633548 10-11 22.498368167841807 27.297590686794255 28.26842727826963 21.93561386709431 12-13 22.533054955468376 26.976649889996292 28.310507011079896 22.179788143455443 14-15 22.183951170969934 27.2264330731454 28.53203152402711 22.057584231857554 16-17 22.38342202785026 27.027264298341546 28.560055457021427 22.029258216786765 18-19 22.433555341818177 27.057385034771436 28.19110882419019 22.317950799220192 20-21 22.380844017958992 27.272542798378364 28.04128505810293 22.30532812555972 22-23 22.416128728998274 27.267548598683934 27.788209587997798 22.528113084319994 24-25 22.455547644815212 27.255358284056875 27.83694266038612 22.45215141074179 26-27 22.542736303727747 27.355727767338085 27.555486532797026 22.546049396137143 28-29 22.58269770153831 27.367567104206618 27.57650875500924 22.47322643924583 30-31 22.52311534013792 27.22964809605794 27.64189883809893 22.605337725705212 32-33 22.471275434880287 27.461698854330375 27.675137540402815 22.39188817038652 34-35 22.439177415898197 27.152853917089338 27.822831543779113 22.585137123233352 36-37 22.61801576235298 27.28891007358553 27.821658720334806 22.271415443726678 38-39 22.384648803654166 27.320287207488235 27.812095035080915 22.48296895377669 40-41 22.293402885985017 27.157029150366125 27.812481169783275 22.73708679386558 42-43 22.32873342436241 27.45038231799464 27.519296052825027 22.701588204817917 44-45 22.229497523774498 27.16747385240056 27.81771600812073 22.785312615704214 46-47 22.337335052053405 27.10170991939617 27.8357897570496 22.725165271500824 48-49 22.422761318580108 27.036603113153085 27.804455123665544 22.736180444601263 50-51 22.207513702084125 27.3308971771584 27.527991807225423 22.933597313532054 52-53 22.240019617175136 27.19472324498563 27.735023989203928 22.83023314863531 54-55 22.285309567372956 27.088369569381754 27.722259671488818 22.904061191756465 56-57 22.082138338472244 27.36853330903654 27.58461598991131 22.964712362579906 58-59 22.26129290101343 27.213789618855532 27.601001420542786 22.92391605958825 60-61 22.19719060021292 26.975754622641567 27.599465148396057 23.227589628749453 62-63 22.239116581919895 26.99271584333586 27.66563001752646 23.102537557217794 64-65 22.314706095528013 26.786630633988757 27.752492777933092 23.14617049255014 66-67 22.180320417310774 26.93758457987809 27.908030933844685 22.974064068966445 68-69 22.32007760501384 26.724315395457936 27.86024553106375 23.095361468464475 70-71 22.121217833680284 26.669129054316777 27.86538956719636 23.34426354480658 72-73 22.208935235886134 26.69051694525831 27.82081545909093 23.27973235976463 74-75 22.24235530083585 26.571954863226292 27.694634724484175 23.491055111453683 76-77 22.07661540960618 26.44350065224706 27.986205844862994 23.493678093283762 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 7903.0 1 4634.5 2 1284.0 3 1234.0 4 1266.0 5 1332.0 6 1398.0 7 1491.5 8 1633.5 9 1682.0 10 1766.5 11 1837.0 12 1925.0 13 2027.0 14 2165.0 15 2412.0 16 2707.0 17 2893.0 18 3171.0 19 3888.0 20 4784.0 21 6051.0 22 6861.0 23 7874.5 24 10468.0 25 14334.0 26 16620.0 27 19977.5 28 27264.5 29 34773.5 30 38353.0 31 43559.0 32 53647.0 33 63972.0 34 73179.0 35 76943.0 36 82528.5 37 93179.5 38 99241.0 39 100237.0 40 102680.0 41 107418.5 42 112877.5 43 116041.0 44 114943.5 45 113568.0 46 115432.0 47 116506.5 48 115439.0 49 114607.5 50 112891.0 51 108522.5 52 105039.0 53 101579.5 54 92495.5 55 81105.5 56 69421.5 57 63503.0 58 60255.5 59 51511.5 60 41569.0 61 37123.0 62 32573.0 63 24040.5 64 17473.0 65 14888.0 66 12851.0 67 9675.0 68 7689.0 69 5670.0 70 4498.0 71 4092.0 72 3313.0 73 2740.0 74 2540.0 75 2057.5 76 1279.0 77 762.5 78 542.0 79 447.5 80 298.5 81 212.0 82 146.0 83 112.0 84 107.0 85 83.0 86 50.0 87 36.0 88 44.5 89 43.5 90 32.0 91 30.0 92 37.0 93 35.5 94 27.0 95 24.5 96 22.0 97 20.5 98 19.5 99 46.0 100 72.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0028571736176041646 2 0.008612929166111104 3 0.011511511097013882 4 0.019586132190243043 5 0.015196850980590267 6 0.014244459774722211 7 0.018136841224791655 8 0.019627540503541655 9 0.0183024744779861 10-11 0.021118239782291652 12-13 0.021118239782291652 14-15 0.0074949047070486064 16-17 0.0054244890421180515 18-19 0.011449398627065965 20-21 0.010372782481302075 22-23 0.01453431796781249 24-25 0.02215344761475693 26-27 0.012898689592517353 28-29 0.00766053796024305 30-31 0.007515608863697912 32-33 0.009482503745381938 34-35 0.011035315494079854 36-37 0.009648136998576383 38-39 0.017101633392326374 40-41 0.012608831399427074 42-43 0.014948401100798602 44-45 0.017060225079027765 46-47 0.016004313089913184 48-49 0.013085027002361102 50-51 0.026977516114045124 52-53 0.03159454304684026 54-55 0.02470005888262151 56-57 0.01610783387315971 58-59 0.016728958572638876 60-61 0.019565428033593738 62-63 0.025859491654982622 64-65 0.020662748336006932 66-67 0.01931697815380207 68-69 0.028861594369131924 70-71 0.054969535903906214 72-73 0.06563217657829858 74-75 0.07718509598861106 76-77 0.07362398104493051 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 2414974.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.17289451035241 #Duplication Level Percentage of deduplicated Percentage of total 1 73.89264926705123 39.290860445673836 2 13.826747485495213 14.704163709350349 3 5.1626155378375485 8.235336341728267 4 2.465480424839305 5.243869221892767 5 1.3300076110589414 3.5360177200401446 6 0.8038981918431655 2.5647356251163806 7 0.4996924189156639 1.859906459503779 8 0.3389539112238831 1.4418528452303112 9 0.24807683855320675 1.187186721016625 >10 1.2764593776154005 12.315744003310021 >50 0.09955111157993857 3.6674086852754675 >100 0.054385403202150996 4.981010854713199 >500 0.0010142879050818168 0.3362491659516159 >1k 3.901107327230443E-4 0.3485292095194473 >5k 7.802214654460885E-5 0.2871289916776687 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6921 0.2865869363396873 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3562 0.14749641196965269 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 5.383080728819441E-4 0.0 3 0.0 0.0 0.0 7.453496393749995E-4 0.0 4 0.0 0.0 0.0 0.0016977408452430545 0.0 5 0.0 0.0 0.0 0.003643931570277775 0.0 6 4.140831329861108E-5 0.0 0.0 0.006376880247986107 0.0 7 4.140831329861108E-5 0.0 0.0 0.01225686073638888 0.0 8 8.281662659722216E-5 0.0 0.0 0.018468107731180544 0.0 9 8.281662659722216E-5 0.0 0.0 0.025300479425451373 0.0 10 8.281662659722216E-5 0.0 0.0 0.03395481690486109 0.0 11 8.281662659722216E-5 0.0 0.0 0.04339591233694441 0.0 12 8.281662659722216E-5 0.0 0.0 0.05681220584569441 0.0 13 8.281662659722216E-5 0.0 0.0 0.07478341381729162 0.0 14 8.281662659722216E-5 0.0 0.0 0.09685404480545132 0.0 15 8.281662659722216E-5 0.0 0.0 0.1210364997718402 0.0 16 8.281662659722216E-5 0.0 0.0 0.1464612041371874 0.0 17 8.281662659722216E-5 0.0 0.0 0.16728958572638877 0.0 18 8.281662659722216E-5 0.0 0.0 0.19292133165822903 0.0 19 8.281662659722216E-5 0.0 0.0 0.2196296937358332 0.0 20 8.281662659722216E-5 0.0 0.0 0.2510171952161804 0.0 21 8.281662659722216E-5 0.0 0.0 0.28459933730135395 0.0 22 8.281662659722216E-5 0.0 0.0 0.31979640360517336 0.0 23 8.281662659722216E-5 0.0 0.0 0.3580990934063886 0.0 24 8.281662659722216E-5 0.0 0.0 0.39590488344802055 0.0 25 8.281662659722216E-5 0.0 0.0 0.4356568642146872 0.0 26 8.281662659722216E-5 0.0 0.0 0.4766925026936108 0.0 27 8.281662659722216E-5 0.0 0.0 0.517769549485833 0.0 28 8.281662659722216E-5 0.0 0.0 0.5608756036296871 0.0 29 8.281662659722216E-5 0.0 0.0 0.6076669976571176 0.0 30 8.281662659722216E-5 0.0 0.0 0.6579366900016315 0.0 31 8.281662659722216E-5 0.0 0.0 0.7141277711478468 0.0 32 8.281662659722216E-5 0.0 0.0 0.7731346175983675 0.0 33 8.281662659722216E-5 0.0 0.0 0.8381870777904855 0.0 34 8.281662659722216E-5 0.0 0.0 0.9014589805107632 0.0 35 8.281662659722216E-5 0.0 0.0 0.9659317243167007 0.0 36 1.2422493989583324E-4 0.0 0.0 1.0351250158386798 0.0 37 1.2422493989583324E-4 0.0 0.0 1.1085419553171172 0.0 38 1.2422493989583324E-4 0.0 0.0 1.18150340334927 0.0 39 1.2422493989583324E-4 0.0 0.0 1.2576118831921173 0.0 40 1.2422493989583324E-4 0.0 0.0 1.3385651356909019 0.0 41 1.2422493989583324E-4 0.0 0.0 1.4260194933775685 0.0 42 1.2422493989583324E-4 0.0 0.0 1.520720305891492 0.0 43 1.2422493989583324E-4 0.0 0.0 1.6203901160012488 0.0 44 1.2422493989583324E-4 0.0 0.0 1.7265610312988877 0.0 45 1.2422493989583324E-4 0.0 0.0 1.8363344698535056 0.0 46 1.6563325319444432E-4 0.0 0.0 1.9544309793811445 0.0 47 1.6563325319444432E-4 0.0 0.0 2.0773308532514223 0.0 48 1.6563325319444432E-4 0.0 0.0 2.2022597344733317 0.0 49 1.6563325319444432E-4 0.0 0.0 2.327975373647915 0.0 50 1.6563325319444432E-4 0.0 0.0 2.461600000662533 0.0 51 1.6563325319444432E-4 0.0 0.0 2.6058251558815955 0.0 52 1.6563325319444432E-4 0.0 0.0 2.7532801595379497 0.0 53 1.6563325319444432E-4 0.0 0.0 2.917961021526526 0.0 54 1.6563325319444432E-4 0.0 0.0 3.0878179226774285 0.0 55 1.6563325319444432E-4 0.0 0.0 3.2632649461236434 0.0 56 1.6563325319444432E-4 0.0 0.0 3.4489812312679144 0.0 57 1.6563325319444432E-4 0.0 0.0 3.641612704733053 0.0 58 1.6563325319444432E-4 0.0 0.0 3.835693469163643 0.0 59 1.6563325319444432E-4 0.0 0.0 4.037351954927879 0.0 60 1.6563325319444432E-4 0.0 0.0 4.237892416233053 0.0 61 1.6563325319444432E-4 0.0 0.0 4.450606921648018 0.0 62 1.6563325319444432E-4 0.0 0.0 4.672886747434962 0.0 63 1.6563325319444432E-4 0.0 0.0 4.904483443714094 0.0 64 1.6563325319444432E-4 0.0 0.0 5.142829695060899 0.0 65 1.6563325319444432E-4 0.0 0.0 5.402459819443191 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTCGG 85 1.1898794E-4 25.057209 11 CGGTTAC 150 5.582224E-7 21.297743 4 GGCGATA 85 0.0034898743 20.885332 59 CTCGCGC 210 3.6436177E-8 18.592113 36 GCGTTCG 115 9.1709086E-4 18.52093 10 TCGCGCT 235 6.675691E-9 18.12574 37 CGCGCTT 275 2.5556801E-9 16.780737 38 CGCGGAA 195 6.8448226E-6 16.386953 59 GACGTAT 630 0.0 16.341232 39 AATTCGC 265 2.9380317E-8 16.0761 55 TGCGTAA 710 0.0 15.998304 37 ACTCGAG 355 1.8189894E-11 15.99665 14 ATACGTA 225 1.524737E-6 15.775453 1 TTGCGTA 725 0.0 15.666332 36 GCGTAAG 735 0.0 15.454786 38 ATTCGCG 255 3.326095E-7 15.313689 56 CGTAAGT 760 0.0 14.947333 39 CCTCGCG 290 8.911593E-8 14.686883 35 ACGTATT 725 0.0 14.199083 40 CGATCGT 305 1.6500417E-7 13.965157 1 >>END_MODULE