FastQCFastQC Report
Thu 26 May 2016
SRR935998_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935998_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2414974
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62660.25946449112909703No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48480.2007475028716665No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35420.14666824570368048No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA11200.047.561363100-101
GGTATCA62400.037.004241
CGGTATC8750.035.370777100-101
ACGTATC4850.031.29304100-101
TACGCAG7400.030.965515100-101
TCGAGTA3300.030.660858100-101
CCGTATC5600.030.290478100-101
GTATCAA117300.029.7813531
TCGTATC4150.029.400524100-101
CGTACAT12350.029.156656100-101
GCGTATC3200.028.829094100-101
TCGGTAT3100.027.839128100-101
CCTGTCG6450.026.7545596-97
TACGGTA1800.026.4494998-99
TACGTAC2850.026.1014798-99
CGACGCA1600.026.039183100-101
CGAGCGT1850.025.73224396-97
CGAGTAC8150.025.559933100-101
CGAGCCG4350.025.30706696-97
GCGTACA3500.024.657513100-101
Produced by FastQC (version 0.11.5)