FastQCFastQC Report
Thu 26 May 2016
SRR935997_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935997_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1542880
Sequences flagged as poor quality0
Sequence length77
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79880.517733070621176No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28010.18154360676138132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTATA1051.769331E-830.42305810
TCGCGGA800.002456470622.18779458
CGTAACG1153.697058E-521.6061828
CGTTACG1003.5794708E-421.29613711
GCGGTAT1355.338821E-621.0332229
CGAGTAG850.003493240620.88127735
CCTCGCG1205.1296836E-520.70726635
ACCGGGT1257.020982E-519.87961836
CGCGCTT1257.024483E-519.87832838
GTAACGG1257.0262344E-519.87768629
GATCGTA1501.3264458E-518.93051525
ATCGATC1000.00892601217.75196363
ACGATAA1000.0089360217.74850818
TCGTTAC1602.3126251E-517.74678210
TACGTAG2258.019197E-817.3518492
TTAGTCG1653.0114727E-517.2095615
TAACGGA1452.1914682E-417.13593530
TAGTCGG1553.6452862E-416.02987116
TAGAGCG2051.1010716E-515.58557245
GGAGCGT1604.6331837E-415.53246350
Produced by FastQC (version 0.11.5)