FastQCFastQC Report
Thu 26 May 2016
SRR935997_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935997_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1542880
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97310.6307036192056413No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71990.4665949393342321No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51170.3316524940371254No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA8650.053.335236100-101
GGTATCA65350.042.6147461
CGGTATC6200.041.766315100-101
CCGTACA4700.041.163677100-101
GCGTATC2450.040.090965100-101
CGAGTAC6900.039.686100-101
CGACGCA1150.038.823265100-101
TACGCAG5400.038.03242100-101
CGTACAT11850.037.425407100-101
ACGTATC3300.036.980137100-101
CCGTATC3750.034.923683100-101
TCGTATC3750.034.129963100-101
GACGCAG5500.034.093887100-101
ACCGTAC1750.034.0138298-99
GCGTACA2800.032.95356100-101
TCGTACA6450.032.764027100-101
GTATCAA133100.032.5471531
TCGGTAT3050.032.204216100-101
AGGTATC16300.031.773151100-101
AGTATCA25450.031.226416100-101
Produced by FastQC (version 0.11.5)