FastQCFastQC Report
Thu 26 May 2016
SRR935996_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935996_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1557639
Sequences flagged as poor quality0
Sequence length77
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79170.5082692459549356No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27790.17841104389399598No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCGA350.001172117840.56559425
TCGCGAT350.001172302940.56429326
TAACGCG753.1242962E-837.8709552
ACGCGAA1055.9936065E-727.04546754
AACGCGA1108.9830064E-725.82027253
CGCGATG700.001130748325.35186827
GCGTCGT1956.548362E-1021.841613
GCGTATA2650.021.43294543
CGCGAAT1505.5798955E-721.2976255
AGCGTCG2101.6516424E-920.2814942
CGTCGTG2152.21371E-919.8104674
GATCGCG900.004858014619.72508661
ACCGTTA1459.8948185E-619.58464811
TAGGGCG1459.900306E-619.5833917
ATAACGC1501.3234665E-518.93547451
CGAACTC4550.018.72258425
CTGCGTA1551.7506323E-518.3305469
TACGTAG1752.4479086E-618.2533452
CGTATAA2559.349606E-1018.09834344
CGGATAA2559.349606E-1018.09601856
Produced by FastQC (version 0.11.5)