##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935996_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1557639 Sequences flagged as poor quality 0 Sequence length 77 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.01098970942561 40.0 38.0 41.0 33.0 41.0 2 37.88657834068099 40.0 38.0 41.0 33.0 41.0 3 37.769417689207835 40.0 38.0 41.0 33.0 41.0 4 37.67550568520691 40.0 37.0 41.0 33.0 41.0 5 37.607645930796544 40.0 37.0 41.0 33.0 41.0 6 37.49559750365778 40.0 37.0 41.0 33.0 41.0 7 37.338881473820315 40.0 37.0 41.0 32.0 41.0 8 37.19078361545904 40.0 36.0 41.0 32.0 41.0 9 37.33366524592669 40.0 37.0 41.0 33.0 41.0 10-11 37.51473351655936 40.0 37.0 41.0 33.0 41.0 12-13 37.43547798944428 40.0 37.0 41.0 33.0 41.0 14-15 37.2854018164671 40.0 37.0 41.0 32.5 41.0 16-17 37.13152469859833 40.0 37.0 41.0 32.0 41.0 18-19 36.94319351274589 40.0 37.0 41.0 31.0 41.0 20-21 36.672077098737255 40.0 36.5 41.0 30.5 41.0 22-23 36.306901984349395 40.0 36.0 41.0 30.0 41.0 24-25 36.0167785988923 40.0 36.0 41.0 29.0 41.0 26-27 35.071499236986234 39.0 35.0 40.0 27.0 40.5 28-29 35.10718786573783 38.5 35.0 40.0 27.0 40.5 30-31 35.52287628905029 39.0 35.0 41.0 28.0 41.0 32-33 35.42290607772404 39.0 35.0 41.0 27.0 41.0 34-35 35.2084234537014 39.0 35.0 41.0 26.5 41.0 36-37 34.94369715961144 39.0 35.0 40.5 26.0 41.0 38-39 34.66731925690099 38.0 34.5 40.0 26.0 41.0 40-41 34.34737028284474 38.0 34.0 40.0 25.0 41.0 42-43 34.000825608501074 37.0 34.0 40.0 23.5 41.0 44-45 33.658495004298175 37.0 34.0 39.5 23.0 41.0 46-47 33.26052345890159 36.0 33.5 39.0 22.0 41.0 48-49 32.853219520055674 36.0 33.0 39.0 21.5 40.0 50-51 32.40564180789002 35.0 33.0 38.0 19.5 40.0 52-53 31.934599737166312 35.0 32.0 37.0 18.0 39.0 54-55 31.485467749587677 35.0 32.0 37.0 14.0 39.0 56-57 31.045752578100576 35.0 31.5 36.0 7.5 38.5 58-59 30.61582979111335 35.0 31.0 36.0 6.5 37.0 60-61 30.24941851096435 34.5 31.0 35.5 2.0 37.0 62-63 29.8909102173225 34.0 31.0 35.0 2.0 36.5 64-65 29.53900550769466 34.0 30.0 35.0 2.0 36.0 66-67 29.201411238419173 34.0 30.0 35.0 2.0 36.0 68-69 28.860928302385854 34.0 29.5 35.0 2.0 35.5 70-71 28.454658942155405 34.0 29.0 35.0 2.0 35.0 72-73 28.03843862409711 34.0 29.0 35.0 2.0 35.0 74-75 27.54723141883325 34.0 29.0 35.0 2.0 35.0 76-77 25.816578488340365 32.0 23.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6249162526764067 1101 2 0.5002642753982087 1101 3 0.5206173911753353 1101 4 0.5624834684063913 1101 5 0.6601794452024947 1101 6 0.7175784162519463 1101 7 0.6524724575349552 1101 8 0.6909138302140292 1101 9 0.6139088776793287 1101 10-11 0.6021245703370184 1101 12-13 0.6402314662434705 1101 14-15 0.6501615899839166 1101 16-17 0.579208419021576 1101 18-19 0.7373430473896221 1101 20-21 0.7070832834464369 1101 22-23 0.6825001711255609 1101 24-25 0.9309593222913577 1101 26-27 0.8377248729743343 1101 28-29 0.8803099617448922 1101 30-31 0.8127789216175714 1101 32-33 0.8811445543026579 1101 34-35 0.7936125143825024 1101 36-37 0.8849023517857475 1101 38-39 0.8986023832357262 1101 40-41 0.8210483505053503 1101 42-43 0.8534030417675567 1101 44-45 0.7468037339183198 1101 46-47 0.8485705180397289 1101 48-49 0.744672711839506 1101 50-51 0.7035884712718712 1101 52-53 0.6933036007102231 1101 54-55 0.7126109272228689 1101 56-57 0.6701039663046195 1101 58-59 0.7219522784702619 1101 60-61 0.7472174291991642 1101 62-63 0.7999365505354028 1101 64-65 0.7050265473050459 1101 66-67 0.8729243170674827 1101 68-69 0.8078637731488527 1101 70-71 0.7601832310260441 1101 72-73 0.8532542240995191 1101 74-75 0.8991880078934997 1101 76-77 1.1485409159679385 1102 1 0.43921398534740064 1102 2 0.5086747984080517 1102 3 0.5325487255753671 1102 4 0.33695872005914396 1102 5 0.38399561050619013 1102 6 0.44877725605033447 1102 7 0.3887436593550433 1102 8 0.309807312958263 1102 9 0.3212900268280521 1102 10-11 0.48681056613566653 1102 12-13 0.38798734987442884 1102 14-15 0.4905468454422035 1102 16-17 0.4944860104768196 1102 18-19 0.43494301328444607 1102 20-21 0.4580697716189448 1102 22-23 0.43346299687981116 1102 24-25 0.5202644176900151 1102 26-27 0.4247374516029865 1102 28-29 0.6425157606409329 1102 30-31 0.7840707371195421 1102 32-33 0.7709398724292029 1102 34-35 0.7101976011140678 1102 36-37 0.6867640169863876 1102 38-39 0.7649556760470091 1102 40-41 0.8150752521326581 1102 42-43 0.6976253245690529 1102 44-45 0.6803661168529018 1102 46-47 0.7365987815271993 1102 48-49 0.6802964629624952 1102 50-51 0.6943452658081526 1102 52-53 0.6985046393534944 1102 54-55 0.7593178030522694 1102 56-57 0.784170706839749 1102 58-59 0.8197023149416722 1102 60-61 0.7245374901973598 1102 62-63 0.7905898953257804 1102 64-65 0.6829708268078782 1102 66-67 0.6723200062833286 1102 68-69 0.6484748971450074 1102 70-71 0.6544440972582883 1102 72-73 0.7654766257997743 1102 74-75 0.8324529483639367 1102 76-77 0.8949977132811391 1103 1 0.26026507674679067 1103 2 0.1687448732657444 1103 3 0.2256250967864588 1103 4 0.18552567820617583 1103 5 0.08926388991199019 1103 6 0.21169288302679234 1103 7 0.13777804543698835 1103 8 0.24090692498206323 1103 9 0.27301523080661383 1103 10-11 0.16008496883167567 1103 12-13 0.2488205547214548 1103 14-15 0.26328302162682604 1103 16-17 0.18540892498675277 1103 18-19 0.2538481211657455 1103 20-21 0.1788057700204746 1103 22-23 0.14451158672950015 1103 24-25 0.21088742156348417 1103 26-27 0.2810558705606425 1103 28-29 0.42454424998810936 1103 30-31 0.35492359250479666 1103 32-33 0.36246178222867087 1103 34-35 0.5533608579115139 1103 36-37 0.43038880183738115 1103 38-39 0.38330650973958313 1103 40-41 0.3867123984150993 1103 42-43 0.38716668352412853 1103 44-45 0.31002485984777906 1103 46-47 0.40190441111534625 1103 48-49 0.36271922171555815 1103 50-51 0.4165513179851388 1103 52-53 0.5231959575600484 1103 54-55 0.5328555764527785 1103 56-57 0.42920906393518266 1103 58-59 0.3780391619821373 1103 60-61 0.36342679426308067 1103 62-63 0.3959102510178276 1103 64-65 0.45172772966908425 1103 66-67 0.5213562016622255 1103 68-69 0.45329462823627864 1103 70-71 0.4897233425769265 1103 72-73 0.4785482849722804 1103 74-75 0.34780876627629453 1103 76-77 0.3945088097780207 1104 1 0.16620634772754528 1104 2 0.33902344609277435 1104 3 0.5112065289938812 1104 4 0.47878037158649533 1104 5 0.3764826833224575 1104 6 0.43007180608984896 1104 7 0.383269960796099 1104 8 0.251525105557171 1104 9 0.1329250924214307 1104 10-11 0.4590360476643056 1104 12-13 0.4363572642430924 1104 14-15 0.5652011621128068 1104 16-17 0.46492687652551723 1104 18-19 0.39394874798568225 1104 20-21 0.30058873400551533 1104 22-23 0.5704713950475266 1104 24-25 0.5976377704010574 1104 26-27 0.3103059785526767 1104 28-29 0.4165062243621449 1104 30-31 0.5341072348973412 1104 32-33 0.5530601435466949 1104 34-35 0.602737106812171 1104 36-37 0.5631383955076501 1104 38-39 0.5604670496065083 1104 40-41 0.6328332313988199 1104 42-43 0.6060981917144304 1104 44-45 0.6125449199504018 1104 46-47 0.4891242922153438 1104 48-49 0.4430248935503016 1104 50-51 0.4339815427017868 1104 52-53 0.3762199675295079 1104 54-55 0.4931529663086742 1104 56-57 0.4980827303501272 1104 58-59 0.32620259821089803 1104 60-61 0.4459678623120489 1104 62-63 0.461783448172433 1104 64-65 0.4585156892567568 1104 66-67 0.49225662213192223 1104 68-69 0.180885504696505 1104 70-71 0.218415504686174 1104 72-73 0.008927259312127234 1104 74-75 -0.21194151574606224 1104 76-77 0.007621917058397543 1105 1 -0.010919184133143744 1105 2 0.13175122838717357 1105 3 0.041052106993554105 1105 4 0.25086721108906573 1105 5 0.12320466118408291 1105 6 0.10760267543452784 1105 7 0.20992796482168075 1105 8 0.1054935911468391 1105 9 0.1682647304897955 1105 10-11 0.3757913095239047 1105 12-13 0.46511190535728275 1105 14-15 0.33749067035736147 1105 16-17 0.25356885698130327 1105 18-19 0.33151172005909046 1105 20-21 0.3198602151550105 1105 22-23 0.3229827502876148 1105 24-25 0.42633693818529395 1105 26-27 0.2710177743367055 1105 28-29 0.5245448773446171 1105 30-31 0.6272057272735267 1105 32-33 0.6653200968981494 1105 34-35 0.40069953318551654 1105 36-37 0.685920909943448 1105 38-39 0.5427868076084508 1105 40-41 0.6221624326060322 1105 42-43 0.6927877194152003 1105 44-45 0.42323957574938476 1105 46-47 0.5467320135469791 1105 48-49 0.35918720457617326 1105 50-51 0.44013051233390854 1105 52-53 0.4248413568410996 1105 54-55 0.2905461830437872 1105 56-57 0.34915131576863345 1105 58-59 0.5551128889189414 1105 60-61 0.5256537373430277 1105 62-63 0.26457865546202086 1105 64-65 0.393419647435195 1105 66-67 0.3738395923986779 1105 68-69 0.22196240532396416 1105 70-71 0.26757483474438004 1105 72-73 -0.38321880578739 1105 74-75 -0.45715158479240614 1105 76-77 -0.2980245499042802 1106 1 -0.11474733393632874 1106 2 0.05504620144904493 1106 3 -0.08713612807589755 1106 4 0.008433637377429193 1106 5 -0.002997528717166631 1106 6 0.009354309983116593 1106 7 -0.2623772082053435 1106 8 -0.08984898970930288 1106 9 0.021494321607974598 1106 10-11 -0.15425001071871947 1106 12-13 0.09136541733919756 1106 14-15 0.048352809700396904 1106 16-17 0.0034190681179566695 1106 18-19 0.1949830501125831 1106 20-21 0.1873913604967541 1106 22-23 0.09346324431634656 1106 24-25 0.005186153912283942 1106 26-27 0.09468644375805724 1106 28-29 0.38314060973457487 1106 30-31 0.516554724583294 1106 32-33 0.45732363931524844 1106 34-35 0.39768585286716984 1106 36-37 0.292540742227672 1106 38-39 0.4878501008628149 1106 40-41 0.3813974232845112 1106 42-43 0.4358019487604423 1106 44-45 0.3833193517464011 1106 46-47 0.36542781205887565 1106 48-49 0.40848982104746767 1106 50-51 0.5817967612627086 1106 52-53 0.6559858631107574 1106 54-55 0.5387594585144555 1106 56-57 0.47145302753905227 1106 58-59 0.3021654498907651 1106 60-61 0.04246350906718632 1106 62-63 0.02019603622172994 1106 64-65 0.23958111386219727 1106 66-67 0.09031075774410269 1106 68-69 0.006492020551583266 1106 70-71 0.16303653056915124 1106 72-73 0.0814590705765248 1106 74-75 0.16521278765790015 1106 76-77 -0.08967957958754269 1107 1 -0.20951412227864097 1107 2 -0.13400067264562665 1107 3 -0.10963453470432682 1107 4 -0.16676075395463386 1107 5 -0.10554576442027752 1107 6 -0.3932553429515835 1107 7 -0.555320020651024 1107 8 -0.37533945845025585 1107 9 -0.5934694654017036 1107 10-11 -0.43527440378900195 1107 12-13 -0.21766870978287756 1107 14-15 -0.4328940759824391 1107 16-17 -0.3250669391825838 1107 18-19 -0.39717060884795785 1107 20-21 -0.42305999821464013 1107 22-23 -0.410771647624081 1107 24-25 -0.32440304592547875 1107 26-27 -0.553410780878373 1107 28-29 -0.49565827780235594 1107 30-31 -0.30517538724242144 1107 32-33 -0.05084832893319913 1107 34-35 -0.23025609046673878 1107 36-37 -0.3048826310848085 1107 38-39 -0.226737650455334 1107 40-41 -0.17298000298583815 1107 42-43 -0.06567670006045745 1107 44-45 -0.18509886483624172 1107 46-47 -0.31550155812479375 1107 48-49 -0.24765537855854092 1107 50-51 -0.1945465958263526 1107 52-53 -0.0689686533734033 1107 54-55 -0.18601833857691474 1107 56-57 -0.135436131740164 1107 58-59 -0.21626493425663185 1107 60-61 -0.33327146978442457 1107 62-63 -0.4500780236890414 1107 64-65 -0.19013613510935556 1107 66-67 0.020710549157275437 1107 68-69 -0.31582445201345166 1107 70-71 -0.28967973340906283 1107 72-73 -0.26017601836257853 1107 74-75 -0.3735061415964047 1107 76-77 -0.14206268405477473 1108 1 0.025715187938445183 1108 2 0.2560548952583517 1108 3 0.4385724742606314 1108 4 0.4846573389549036 1108 5 0.4112942741068011 1108 6 0.381184903029002 1108 7 0.38201420176876866 1108 8 0.39964683480010166 1108 9 0.3533542732068824 1108 10-11 0.3148348797554874 1108 12-13 0.3923990830599777 1108 14-15 0.3913238983822396 1108 16-17 0.3798745292918966 1108 18-19 0.12336839982063452 1108 20-21 0.2898476849742124 1108 22-23 0.3178047026759785 1108 24-25 0.3061567080939298 1108 26-27 0.29019001929945176 1108 28-29 0.3370652903979803 1108 30-31 0.3442037340437878 1108 32-33 0.27889679961388225 1108 34-35 0.3311242162680941 1108 36-37 0.41125354732520947 1108 38-39 0.5446419153741147 1108 40-41 0.41821618876046784 1108 42-43 0.424795997576048 1108 44-45 0.32581207833630543 1108 46-47 0.2892276409564758 1108 48-49 0.32311256987444636 1108 50-51 0.30316808150935515 1108 52-53 0.39429503485361295 1108 54-55 0.4022990652906131 1108 56-57 0.5348257783139232 1108 58-59 0.522878579116707 1108 60-61 0.43940801454542466 1108 62-63 0.30451040336882684 1108 64-65 0.30661647658213553 1108 66-67 0.4113225370064306 1108 68-69 0.4447893880991671 1108 70-71 0.44199693548032215 1108 72-73 0.5094016655671858 1108 74-75 0.5226837524541281 1108 76-77 0.48354967424706885 1109 1 -0.21674559542595517 1109 2 -0.25553307588595686 1109 3 -0.3890074719980561 1109 4 -0.15728055593482537 1109 5 -0.2937215820020356 1109 6 -0.3459841158288697 1109 7 -0.49006724537349555 1109 8 -0.3008240069050956 1109 9 -0.28202482093725223 1109 10-11 -0.38815281663028856 1109 12-13 -0.2060699249043907 1109 14-15 -0.16542145372020656 1109 16-17 -0.26183036552522054 1109 18-19 -0.20858937572464953 1109 20-21 -0.20904934714953072 1109 22-23 -0.28606642710203545 1109 24-25 -0.2587534809689842 1109 26-27 -0.3293367484751215 1109 28-29 -0.26451366334091375 1109 30-31 -0.09429029873357564 1109 32-33 -0.2597970988101892 1109 34-35 -0.13627544240193146 1109 36-37 -0.04178332115381522 1109 38-39 -0.17735571226151592 1109 40-41 -0.10545450043558446 1109 42-43 0.07302792040158579 1109 44-45 -0.08255686063582601 1109 46-47 -0.18000575854483714 1109 48-49 -0.010102498270512683 1109 50-51 -0.20414343050981643 1109 52-53 -0.25326058256631967 1109 54-55 -0.16406964370742827 1109 56-57 -0.10185902402939107 1109 58-59 -0.06960126788999688 1109 60-61 -0.09756939957740585 1109 62-63 -0.16550294118078668 1109 64-65 -0.15475922241808604 1109 66-67 -0.2557922261202492 1109 68-69 -0.18824381895014852 1109 70-71 -0.1345443448702106 1109 72-73 -0.14733173393413423 1109 74-75 -0.2507751184940936 1109 76-77 -0.13887749053542464 1110 1 -0.48345351621803445 1110 2 -0.49860089170368127 1110 3 -0.7742931692424762 1110 4 -0.7768812358823425 1110 5 -0.6607739498894247 1110 6 -0.6262089756642553 1110 7 -0.5044072787744298 1110 8 -0.8486946975886269 1110 9 -0.7483763657302873 1110 10-11 -0.678036360707587 1110 12-13 -0.6169677240216558 1110 14-15 -0.46069984451109747 1110 16-17 -0.6010928610518746 1110 18-19 -0.6390818903375575 1110 20-21 -0.8577058543665217 1110 22-23 -0.8824803004170789 1110 24-25 -0.7180759192369024 1110 26-27 -0.7779507589725583 1110 28-29 -0.5948130788246289 1110 30-31 -0.5842133573044848 1110 32-33 -0.5991542800102323 1110 34-35 -0.5340074595795556 1110 36-37 -0.586379527762432 1110 38-39 -0.6583112174505885 1110 40-41 -0.5080475458847076 1110 42-43 -0.5487289088958036 1110 44-45 -0.38339933946202365 1110 46-47 -0.5756293419875149 1110 48-49 -0.5395557071456167 1110 50-51 -0.5407003015583669 1110 52-53 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-0.2874612370464149 2314 5 -0.4242699629270845 2314 6 -0.3657322862980905 2314 7 -0.4571865116479543 2314 8 -0.5339516674972415 2314 9 -0.6588603305751732 2314 10-11 -0.8080346145926924 2314 12-13 -0.7334306772839838 2314 14-15 -0.9047506855564365 2314 16-17 -0.7786429163455111 2314 18-19 -1.0134409206617505 2314 20-21 -0.9766271918905289 2314 22-23 -0.7807123590865359 2314 24-25 -0.8285686146562625 2314 26-27 -0.8848369995872005 2314 28-29 -1.0079518658568176 2314 30-31 -1.255671654260432 2314 32-33 -1.3273656763201132 2314 34-35 -1.313611382517685 2314 36-37 -2.021048638989953 2314 38-39 -1.633348322392095 2314 40-41 -1.3455206266134212 2314 42-43 -1.1902660720411333 2314 44-45 -1.1890283773527202 2314 46-47 -1.0122330030084115 2314 48-49 -1.0051026776362733 2314 50-51 -0.9789482207714499 2314 52-53 -0.8028970679539924 2314 54-55 -0.9888532090204833 2314 56-57 -0.9694721440370415 2314 58-59 -0.9134454349153955 2314 60-61 -0.8819956682912355 2314 62-63 -0.7507181203073934 2314 64-65 -0.553840570770312 2314 66-67 -0.7390050854496621 2314 68-69 -0.7895212021232467 2314 70-71 -0.69013427886361 2314 72-73 -0.6884961764653426 2314 74-75 -0.749110006330703 2314 76-77 -0.7432967639845067 2315 1 -1.084986083267843 2315 2 -1.0951849290023006 2315 3 -0.9230444963901476 2315 4 -0.8332230783479915 2315 5 -0.9118664424650973 2315 6 -0.8767129070988062 2315 7 -1.333179070923869 2315 8 -1.1816833572079446 2315 9 -0.8986918037274876 2315 10-11 -1.0182420108945394 2315 12-13 -1.0686103096899942 2315 14-15 -0.96193389950502 2315 16-17 -0.9513725237640287 2315 18-19 -0.7783258393135313 2315 20-21 -0.7660345715340426 2315 22-23 -0.7925050738402675 2315 24-25 -0.8997066567463321 2315 26-27 -0.8779411419604557 2315 28-29 -0.8657000750855346 2315 30-31 -0.7503826321048379 2315 32-33 -0.8514369740046064 2315 34-35 -0.8895734015081871 2315 36-37 -0.9734057937458971 2315 38-39 -0.729699452382647 2315 40-41 -0.8692240526781703 2315 42-43 -0.8147316170464123 2315 44-45 -0.7955063883472135 2315 46-47 -0.7889869871274655 2315 48-49 -0.6472989406158973 2315 50-51 -0.5786032543657669 2315 52-53 -0.6549224441547778 2315 54-55 -0.6611628875145712 2315 56-57 -0.8280983864713818 2315 58-59 -0.6725658746955041 2315 60-61 -0.6250199894639827 2315 62-63 -0.6173577171756293 2315 64-65 -0.54765616297421 2315 66-67 -0.460613031476651 2315 68-69 -0.5139818884467502 2315 70-71 -0.47924666711393016 2315 72-73 -0.4790939331425612 2315 74-75 -0.32433628208171683 2315 76-77 -0.4287734700759067 2316 1 -1.4126570560410414 2316 2 -1.3008523755150279 2316 3 -1.2606669830819044 2316 4 -2.5125424648690853 2316 5 -1.7762269121810732 2316 6 -1.7061837419861803 2316 7 -1.2750438071889718 2316 8 -1.1235480934730475 2316 9 -1.3471004656698682 2316 10-11 -1.2617828510939049 2316 12-13 -1.9160543359561188 2316 14-15 -2.8994628611795363 2316 16-17 -1.9814319163882672 2316 18-19 -1.5312869939377904 2316 20-21 -1.2809830338810428 2316 22-23 -1.6154404596380303 2316 24-25 -1.6094244993982585 2316 26-27 -1.3909851976120748 2316 28-29 -2.012582386142391 2316 30-31 -2.0138265001413274 2316 32-33 -2.134754201821373 2316 34-35 -1.9424376404801507 2316 36-37 -1.965225886649307 2316 38-39 -1.9597639309594967 2316 40-41 -1.565947286439851 2316 42-43 -1.800057231033911 2316 44-45 -1.7312962367858304 2316 46-47 -1.834250552493259 2316 48-49 -1.589614348791848 2316 50-51 -1.7354865261025623 2316 52-53 -1.66381579737984 2316 54-55 -1.7699175072548456 2316 56-57 -1.7574728708123004 2316 58-59 -1.8003250039804186 2316 60-61 -1.5484806411440424 2316 62-63 -1.4916414455179456 2316 64-65 -1.6219704070321228 2316 66-67 -1.5140494401159863 2316 68-69 -1.7069386917560117 2316 70-71 -1.5946328039373547 2316 72-73 -1.5543420146082205 2316 74-75 -1.5556595339267858 2316 76-77 -2.0634947033629025 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 19492.0 3 2089.0 4 3242.0 5 4570.0 6 5046.0 7 5359.0 8 7004.0 9 7861.0 10 11559.0 11 14607.0 12 9641.0 13 6278.0 14 5534.0 15 5351.0 16 5121.0 17 5310.0 18 5514.0 19 5817.0 20 6123.0 21 6591.0 22 7372.0 23 8182.0 24 9513.0 25 10984.0 26 13237.0 27 16021.0 28 19587.0 29 23621.0 30 29890.0 31 37600.0 32 48719.0 33 66452.0 34 94501.0 35 133672.0 36 199779.0 37 294677.0 38 305815.0 39 94874.0 40 1034.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 21.048515914308755 27.114417639535898 31.1565544763969 20.68051196975845 2 20.642748454283385 27.20846468446836 31.27028050824061 20.878506353007648 3 20.886852528759913 27.541392636614802 30.609780136028913 20.96197469859637 4 20.503625070552587 27.487672962194086 30.997997237613404 21.01070472963992 5 20.763191249195792 27.571377021433975 30.97935450234167 20.68607722702856 6 20.943643125349933 27.433236250443038 30.950757400644136 20.67236322356289 7 20.978015613422045 27.158082366644447 31.02470947965105 20.83919254028245 8 20.649908370458174 27.58479424769385 30.719076795444593 21.046220586403383 9 20.701286209352066 27.328882659000737 30.91249525641676 21.057335875230436 10-11 21.0394965939698 27.51398804927595 30.791656004595097 20.654859352159153 12-13 21.085057226223377 27.30420309658595 30.67417396977387 20.936565707416808 14-15 20.785856773864655 27.710409412894705 30.58237342241495 20.92136039082569 16-17 21.115469808352863 27.44017848544188 30.66254694873359 20.78180475747167 18-19 20.919552407231727 27.45244206447429 30.420866259910078 21.207139268383905 20-21 20.985234674389467 27.717706929811975 30.25962356172669 21.03743483407187 22-23 21.16879339360897 27.84925012890065 29.693027036710518 21.288929440779864 24-25 21.071745515584205 28.05278684271699 29.477255885574994 21.39821175612381 26-27 21.322493569468364 28.169324277295566 29.23175854332888 21.27642360990719 28-29 21.348329395449177 28.31735708048693 29.15176049103703 21.182553033026863 30-31 21.18234191685623 28.257440198161305 29.192677585313564 21.367540299668896 32-33 21.231048178429305 28.479802915180848 29.00564493657151 21.283503969818337 34-35 21.189585707635754 28.263471323777267 29.181624103115624 21.365318865471355 36-37 21.24725039775332 28.346745870620698 29.253873841250517 21.152129890375463 38-39 21.218379748656083 28.29423367844108 29.361051966497755 21.126334606405084 40-41 21.08497690940815 28.20660662203181 29.304810259060872 21.403606209499166 42-43 21.03563299206051 28.482218819125404 29.00979513999249 21.472353048821596 44-45 21.192155251156215 28.331249127121595 29.065446215440033 21.411149406282156 46-47 21.021273043656937 28.2541720978316 29.17700995909771 21.547544899413758 48-49 21.144049962293277 28.370578288567366 29.022395771966934 21.462975977172423 50-51 20.982415492193475 28.54690689772756 28.87990352056192 21.590774089517044 52-53 20.938378003474284 28.46476426559976 28.946604544826943 21.650253186099008 54-55 20.998656937193914 28.420981318808693 28.89036298656327 21.68999875743412 56-57 20.957292945831625 28.582344112430075 28.723700368399506 21.73666257333879 58-59 21.118689026064025 28.578991656802323 28.724300129095308 21.578019188038343 60-61 21.095738324514308 28.468308169059487 28.66736636693425 21.768587139491956 62-63 20.886148293942185 28.490699921977598 28.81319252911392 21.8099592549663 64-65 21.016912366228695 28.35245376357619 28.776001366448302 21.854632503746814 66-67 20.969059322496445 28.43363823454812 28.84555988274841 21.75174256020702 68-69 21.088144424900605 28.318733251005177 28.81234896604959 21.780773358044623 70-71 20.845038667347122 28.218796900959624 28.91336066924267 22.022803762450586 72-73 21.071611660664338 28.06044899904794 28.86087016853204 22.007069171755692 74-75 20.871016104073995 28.069294001585 28.7531493549986 22.306540539342404 76-77 20.80454944222561 27.968192709469363 29.037805047675917 22.189452800629102 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 8178.0 1 4960.5 2 1754.0 3 1835.5 4 1906.0 5 1960.5 6 2058.5 7 2225.5 8 2394.5 9 2440.0 10 2485.0 11 2663.5 12 2911.0 13 3025.0 14 3161.5 15 3521.5 16 3999.0 17 4253.0 18 4546.5 19 5377.5 20 6648.0 21 8657.0 22 9933.0 23 11208.5 24 13981.0 25 17546.5 26 19615.0 27 21947.5 28 26921.0 29 31437.5 30 33313.0 31 37025.0 32 44220.5 33 50472.0 34 55810.5 35 58381.0 36 61036.0 37 65253.0 38 67146.0 39 67477.0 40 67529.5 41 68138.5 42 69564.0 43 70433.0 44 70053.5 45 69573.0 46 68694.0 47 67805.5 48 67695.0 49 66635.5 50 63805.0 51 59179.5 52 56325.0 53 53684.5 54 48148.5 55 41822.5 56 35915.5 57 33439.0 58 31310.0 59 26107.5 60 20959.5 61 18885.0 62 16519.5 63 11887.5 64 8215.5 65 6810.0 66 5844.5 67 4356.5 68 3352.5 69 2370.5 70 1870.0 71 1618.5 72 1223.5 73 1023.5 74 967.0 75 883.5 76 648.5 77 393.5 78 290.0 79 255.5 80 194.0 81 154.5 82 115.5 83 89.0 84 76.0 85 52.0 86 38.0 87 35.0 88 37.0 89 31.0 90 21.0 91 19.0 92 21.5 93 18.0 94 12.5 95 11.5 96 10.0 97 14.0 98 16.0 99 30.5 100 47.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0030173872123129943 2 0.006997770343449285 3 0.01129915211419334 4 0.01861792109725039 5 0.013160944223918378 6 0.013289343679761485 7 0.016756128987525352 8 0.018040123545956414 9 0.017462325994662433 10-11 0.02102541089430863 12-13 0.019966115383603007 14-15 0.007447168438900156 16-17 0.005713775785018223 18-19 0.01261524653658518 20-21 0.011074453066467904 22-23 0.015279535245329632 24-25 0.022919302867994446 26-27 0.01444493878234944 28-29 0.007639767622664816 30-31 0.007703967350586369 32-33 0.00879536272525277 34-35 0.010528755379134704 36-37 0.009052161636938982 38-39 0.01611413170830982 40-41 0.011973249257369646 42-43 0.015022736333643419 44-45 0.01871422068913272 46-47 0.017269726810897775 48-49 0.014412838918388663 50-51 0.026707086815366078 52-53 0.031072668314031687 54-55 0.024235397290386283 56-57 0.014541238374231769 58-59 0.01685242857940768 60-61 0.019613016880034462 62-63 0.02529469280109191 64-65 0.020351313751132322 66-67 0.019516717288152133 68-69 0.029275075932228202 70-71 0.053574672950536036 72-73 0.06554792220790567 74-75 0.07508158180425631 76-77 0.07321978969453127 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 1557639.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.77792628803232 #Duplication Level Percentage of deduplicated Percentage of total 1 80.76435012755803 53.12511469391345 2 11.948823429883046 15.719376535991211 3 3.4941820566160597 6.89520149271169 4 1.3840012135446345 3.6414691922834503 5 0.67367935729118 2.215661555283411 6 0.4110645905670569 1.6223385802764037 7 0.24195761659327636 1.1140829188370365 8 0.17675221460509732 0.9301115314832449 9 0.12439265138932049 0.7364061588473662 >10 0.7075636054205892 8.659049308653675 >50 0.05216101593312059 2.3418051850854047 >100 0.020877026266056562 2.3130793212401612 >500 0.0 0.0 >1k 9.75471662653075E-5 0.17831315417620003 >5k 9.75471662653075E-5 0.5079903712173355 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7917 0.5082692459549356 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2779 0.17841104389399598 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 2.567989116862123E-4 0.0 3 0.0 0.0 0.0 2.567989116862123E-4 0.0 4 0.0 0.0 0.0 7.703967350586368E-4 0.0 5 6.419972792155307E-5 0.0 0.0 0.0017333926538819328 0.0 6 6.419972792155307E-5 0.0 0.0 0.0030173872123129943 0.0 7 6.419972792155307E-5 0.0 0.0 0.005392777145410458 0.0 8 6.419972792155307E-5 0.0 0.0 0.008538563813566559 0.0 9 6.419972792155307E-5 0.0 0.0 0.012005349121330423 0.0 10 1.2839945584310614E-4 0.0 0.0 0.016884528443368456 0.0 11 3.209986396077653E-4 0.0 0.0 0.021635308309563384 0.0 12 3.851983675293184E-4 0.0 0.0 0.02882567783677733 0.0 13 3.851983675293184E-4 0.0 0.0 0.03807043865748097 0.0 14 5.777975512939776E-4 0.0 0.0 0.04904859213206655 0.0 15 5.777975512939776E-4 0.0 0.0 0.06349353091441598 6.419972792155307E-5 16 5.777975512939776E-4 0.0 0.0 0.07736067214547145 6.419972792155307E-5 17 5.777975512939776E-4 0.0 0.0 0.08936602126680188 6.419972792155307E-5 18 5.777975512939776E-4 0.0 0.0 0.10162816929981851 6.419972792155307E-5 19 6.419972792155306E-4 0.0 0.0 0.11742130236852057 6.419972792155307E-5 20 7.061970071370838E-4 0.0 0.0 0.13315023570930107 6.419972792155307E-5 21 7.061970071370838E-4 0.0 0.0 0.15106195979941436 1.925991837646592E-4 22 8.98796190901743E-4 0.0 0.0 0.1705786770875665 1.925991837646592E-4 23 0.0010271956467448491 0.0 0.0 0.18990279519195397 1.925991837646592E-4 24 0.001091395374666402 0.0 0.0 0.212308500236576 1.925991837646592E-4 25 0.001091395374666402 0.0 0.0 0.23741059385390326 1.925991837646592E-4 26 0.001091395374666402 0.0 0.0 0.2652732757718573 1.925991837646592E-4 27 0.001091395374666402 0.0 0.0 0.29294335850604664 1.925991837646592E-4 28 0.001091395374666402 0.0 0.0 0.3197146450493343 2.567989116862123E-4 29 0.001091395374666402 0.0 0.0 0.34725632832768055 2.567989116862123E-4 30 0.001091395374666402 0.0 0.0 0.37909939337677084 2.567989116862123E-4 31 0.001091395374666402 0.0 0.0 0.4122264529842923 2.567989116862123E-4 32 0.0012839945584310613 0.0 0.0 0.44496831422428434 2.567989116862123E-4 33 0.0012839945584310613 0.0 0.0 0.48258935478631443 2.567989116862123E-4 34 0.0012839945584310613 0.0 0.0 0.522585785281442 2.567989116862123E-4 35 0.0012839945584310613 0.0 0.0 0.5699009847596266 2.567989116862123E-4 36 0.0012839945584310613 0.0 0.0 0.6170877847819681 2.567989116862123E-4 37 0.0012839945584310613 0.0 0.0 0.6675487709283088 2.567989116862123E-4 38 0.0012839945584310613 0.0 0.0 0.7168541619720615 3.209986396077653E-4 39 0.0012839945584310613 0.0 0.0 0.7698189375073429 3.209986396077653E-4 40 0.0012839945584310613 0.0 0.0 0.8243245065127414 3.209986396077653E-4 41 0.0012839945584310613 0.0 0.0 0.881398064635002 3.209986396077653E-4 42 0.0012839945584310613 0.0 0.0 0.9457903917403198 3.209986396077653E-4 43 0.0012839945584310613 0.0 0.0 1.018592883203361 3.209986396077653E-4 44 0.0012839945584310613 0.0 0.0 1.0914595743943236 3.209986396077653E-4 45 0.0012839945584310613 0.0 0.0 1.1675362519813641 3.209986396077653E-4 46 0.0012839945584310613 0.0 0.0 1.2506106999118538 3.209986396077653E-4 47 0.0012839945584310613 6.419972792155307E-5 0.0 1.3368309345104996 3.209986396077653E-4 48 0.0012839945584310613 6.419972792155307E-5 0.0 1.4243993633954979 3.209986396077653E-4 49 0.0012839945584310613 6.419972792155307E-5 0.0 1.512866588471398 3.209986396077653E-4 50 0.0012839945584310613 6.419972792155307E-5 0.0 1.608524183074512 3.209986396077653E-4 51 0.0012839945584310613 6.419972792155307E-5 0.0 1.7043101771334692 3.209986396077653E-4 52 0.0012839945584310613 6.419972792155307E-5 0.0 1.8087631344618362 3.209986396077653E-4 53 0.0012839945584310613 6.419972792155307E-5 0.0 1.9219472547875343 3.209986396077653E-4 54 0.0012839945584310613 6.419972792155307E-5 0.0 2.0466873261391116 3.209986396077653E-4 55 0.0012839945584310613 6.419972792155307E-5 0.0 2.1757287792614335 3.209986396077653E-4 56 0.0013481942863526144 6.419972792155307E-5 0.0 2.312281600550577 3.209986396077653E-4 57 0.0014123940142741675 6.419972792155307E-5 0.0 2.4510814123169746 3.209986396077653E-4 58 0.0014765937421957205 6.419972792155307E-5 0.0 2.5974567919781157 3.851983675293184E-4 59 0.0014765937421957205 6.419972792155307E-5 0.0 2.754104128106705 4.493980954508715E-4 60 0.0014765937421957205 6.419972792155307E-5 0.0 2.906963680287923 4.493980954508715E-4 61 0.001861792109725039 6.419972792155307E-5 0.0 3.0670136019963548 4.493980954508715E-4 62 0.001861792109725039 6.419972792155307E-5 0.0 3.231493304931374 4.493980954508715E-4 63 0.001861792109725039 6.419972792155307E-5 0.0 3.405667166782547 4.493980954508715E-4 64 0.001861792109725039 6.419972792155307E-5 0.0 3.584848607411602 4.493980954508715E-4 65 0.001861792109725039 6.419972792155307E-5 0.0 3.7788601851905352 4.493980954508715E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTCGCGA 35 0.0011721178 40.565594 25 TCGCGAT 35 0.0011723029 40.564293 26 TAACGCG 75 3.1242962E-8 37.87095 52 ACGCGAA 105 5.9936065E-7 27.045467 54 AACGCGA 110 8.9830064E-7 25.820272 53 CGCGATG 70 0.0011307483 25.351868 27 GCGTCGT 195 6.548362E-10 21.84161 3 GCGTATA 265 0.0 21.432945 43 CGCGAAT 150 5.5798955E-7 21.29762 55 AGCGTCG 210 1.6516424E-9 20.281494 2 CGTCGTG 215 2.21371E-9 19.810467 4 GATCGCG 90 0.0048580146 19.725086 61 ACCGTTA 145 9.8948185E-6 19.584648 11 TAGGGCG 145 9.900306E-6 19.583391 7 ATAACGC 150 1.3234665E-5 18.935474 51 CGAACTC 455 0.0 18.722584 25 CTGCGTA 155 1.7506323E-5 18.33054 69 TACGTAG 175 2.4479086E-6 18.253345 2 CGTATAA 255 9.349606E-10 18.098343 44 CGGATAA 255 9.349606E-10 18.096018 56 >>END_MODULE