FastQCFastQC Report
Thu 26 May 2016
SRR935996_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935996_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1557639
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98130.6299919300942003No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71200.4571020628014578No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52110.334544782199213No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA9000.050.595417100-101
CGGTATC6350.044.525845100-101
GGTATCA69450.041.042141
GCGTATC2000.038.690613100-101
TCGTATC3250.036.63017100-101
TACGCAG6150.035.811203100-101
ACGTATC3450.035.36935100-101
CGTACAT11350.035.137527100-101
CGAGTAC6800.035.014133100-101
CCGTATC3950.034.659557100-101
TCGGTAT2550.033.846992100-101
CGATCTA1150.033.644012100-101
AGTATCA28850.033.527397100-101
ACCGTAC1350.033.0662598-99
CCGTACA3700.032.175148100-101
ACGGTAT2600.032.0514100-101
GTATCAA140950.032.043761
AGGTATC17900.031.424692100-101
TGTATCA19850.030.886522100-101
GCGTACA3350.030.206223100-101
Produced by FastQC (version 0.11.5)