FastQCFastQC Report
Thu 26 May 2016
SRR935995_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935995_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1430459
Sequences flagged as poor quality0
Sequence length125
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGAGT650.001941466936.6061257
GTGCTAG2800.036.182891
CTACGGG1754.456524E-1033.9961553
TAAGCGT452.3954693E-433.05181522-23
GTAAGCG2600.032.034843
TAGGCCG750.003915648431.725315
ACGAGTC750.003915648431.725318
AGAGTTG20350.030.4001735
TCTAGGG4750.030.0618612
GTACAGG8600.029.797681
GCGTCAG800.005320818629.7976781
TAAGCGG2601.8189894E-1229.7466354
GGTATCA315450.029.3395421
ACAACAC10300.028.876195
TCTACGG1653.410023E-728.8472372
GAGTTAA8100.028.6940611
GAGTAAG19650.028.508721
CGCAGAA7350.028.332112
GTGCAAG3600.028.1422541
AGTTTCG6250.027.6010197
Produced by FastQC (version 0.11.5)