##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935995_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1430459 Sequences flagged as poor quality 0 Sequence length 125 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.233905340873104 31.0 31.0 34.0 26.0 34.0 2 30.254324660825652 33.0 31.0 34.0 26.0 34.0 3 30.232033913589973 33.0 31.0 34.0 25.0 34.0 4 33.4261562197868 37.0 35.0 37.0 26.0 37.0 5 33.6891571167017 37.0 35.0 37.0 28.0 37.0 6 33.798226303585075 37.0 35.0 37.0 29.0 37.0 7 33.75624677114129 37.0 35.0 37.0 29.0 37.0 8 33.60348321762455 37.0 35.0 37.0 27.0 37.0 9 34.93554236786933 39.0 37.0 39.0 25.0 39.0 10-11 34.96124880195797 39.0 37.0 39.0 27.0 39.0 12-13 34.95384348660116 39.0 37.0 39.0 26.5 39.0 14-15 36.10826070513031 40.0 37.0 41.0 27.0 41.0 16-17 36.19531493038249 40.0 36.5 41.0 27.0 41.0 18-19 36.38868503046925 40.0 36.5 41.0 27.5 41.0 20-21 36.49828970980643 40.0 37.0 41.0 29.0 41.0 22-23 36.27030694343564 40.0 36.0 41.0 28.0 41.0 24-25 36.10278798623379 40.0 36.0 41.0 27.5 41.0 26-27 35.98436341062554 40.0 35.0 41.0 28.0 41.0 28-29 35.81264859740824 40.0 35.0 41.0 27.0 41.0 30-31 35.62330098241194 40.0 35.0 41.0 27.0 41.0 32-33 35.398785634541085 40.0 35.0 41.0 26.5 41.0 34-35 35.12992682768258 40.0 35.0 41.0 24.5 41.0 36-37 34.9848814261716 40.0 35.0 41.0 23.0 41.0 38-39 34.906669118094264 40.0 35.0 41.0 23.0 41.0 40-41 34.737294113288115 40.0 35.0 41.0 21.5 41.0 42-43 34.51970346581062 39.5 35.0 41.0 18.5 41.0 44-45 34.31166220073417 39.0 35.0 41.0 14.5 41.0 46-47 34.086620448401526 39.0 34.5 41.0 8.5 41.0 48-49 33.80598640016946 39.0 34.0 41.0 2.0 41.0 50-51 33.518965241226766 38.0 34.0 41.0 2.0 41.0 52-53 33.25658931853342 37.5 34.0 41.0 2.0 41.0 54-55 32.97240256449154 37.0 33.5 40.0 2.0 41.0 56-57 32.67370787977845 36.5 33.0 40.0 2.0 41.0 58-59 32.33429130090411 36.0 33.0 40.0 2.0 41.0 60-61 32.02659251331216 35.5 33.0 39.5 2.0 41.0 62-63 31.687213335020438 35.0 33.0 39.0 2.0 41.0 64-65 31.397198381778153 35.0 32.0 39.0 2.0 41.0 66-67 31.183585828045402 35.0 32.5 37.5 2.0 40.5 68-69 30.9825412682223 35.0 33.0 37.0 2.0 40.0 70-71 30.68319434531154 35.0 32.5 36.5 2.0 39.0 72-73 30.385443064079432 35.0 32.0 36.0 2.0 39.0 74-75 30.089033659825272 35.0 32.0 35.5 2.0 38.5 76-77 29.827602538765532 35.0 32.0 35.0 2.0 37.0 78-79 29.573774222120313 35.0 31.5 35.0 2.0 37.0 80-81 29.29615459093899 35.0 31.0 35.0 2.0 36.0 82-83 29.06256453348191 35.0 31.0 35.0 2.0 36.0 84-85 28.87318930497134 35.0 31.0 35.0 2.0 36.0 86-87 28.70077296867649 35.0 31.0 35.0 2.0 35.0 88-89 28.4622386940136 35.0 30.0 35.0 2.0 35.0 90-91 28.210469506640877 35.0 29.0 35.0 2.0 35.0 92-93 27.932661124855727 34.5 29.0 35.0 2.0 35.0 94-95 27.693352623178995 34.0 28.5 35.0 2.0 35.0 96-97 27.484179553555887 34.0 27.0 35.0 2.0 35.0 98-99 27.22797507653138 34.0 26.5 35.0 2.0 35.0 100-101 26.99642562282456 34.0 25.0 35.0 2.0 35.0 102-103 26.283033278129608 33.0 24.0 35.0 2.0 35.0 104-105 26.71986404363914 34.0 25.0 35.0 2.0 35.0 106-107 26.72569853452633 34.0 25.0 35.0 2.0 35.0 108-109 26.55537558224318 34.0 24.5 35.0 2.0 35.0 110-111 26.27719948631873 34.0 24.0 35.0 2.0 35.0 112-113 25.898790877613408 34.0 21.0 35.0 2.0 35.0 114-115 25.422516478976327 33.5 17.0 35.0 2.0 35.0 116-117 24.907762822981994 33.0 4.5 35.0 2.0 35.0 118-119 24.19092543022904 33.0 2.0 35.0 2.0 35.0 120-121 23.438478488373313 33.0 2.0 35.0 2.0 35.0 122-123 22.661287740508463 32.5 2.0 35.0 2.0 35.0 124-125 19.972767132787446 27.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.581425418076801 1101 2 0.8191939477322379 1101 3 0.6014601219625604 1101 4 0.7663120316316352 1101 5 0.6896405546759752 1101 6 0.7052000327082979 1101 7 0.6774522486160066 1101 8 0.7111337118906391 1101 9 0.6339810726648665 1101 10-11 0.7935160684641716 1101 12-13 0.6294560984023221 1101 14-15 0.7645000899217891 1101 16-17 0.805584313128989 1101 18-19 0.8125457696360741 1101 20-21 0.767684433317271 1101 22-23 0.8262055181425012 1101 24-25 0.8150360519856577 1101 26-27 0.8772327856728026 1101 28-29 1.0412815254680439 1101 30-31 0.7971362494954519 1101 32-33 0.8629977722322764 1101 34-35 0.6826990617653834 1101 36-37 0.7130168577892491 1101 38-39 0.8433312145342597 1101 40-41 0.797728722151021 1101 42-43 0.7304498369319248 1101 44-45 0.8243002517937228 1101 46-47 0.7732888486737934 1101 48-49 0.7105829652920406 1101 50-51 0.8075487038240183 1101 52-53 0.8551688192856801 1101 54-55 0.7083592334698707 1101 56-57 0.7392196087607488 1101 58-59 0.7139114555340313 1101 60-61 0.7072757135300591 1101 62-63 0.7489586520953608 1101 64-65 0.6783875235472294 1101 66-67 0.5576218403463962 1101 68-69 0.7329937238689048 1101 70-71 0.6044607180391708 1101 72-73 0.7113974569454413 1101 74-75 0.7764702647165436 1101 76-77 0.7839455948606826 1101 78-79 0.927388658059229 1101 80-81 0.8470853717616684 1101 82-83 0.680312034693447 1101 84-85 0.7873029426200411 1101 86-87 0.7098525242448623 1101 88-89 0.6298380633258809 1101 90-91 0.6897822566571712 1101 92-93 0.4501607432584258 1101 94-95 0.5314041578104849 1101 96-97 0.522078575209445 1101 98-99 0.5508460464160727 1101 100-101 0.7376229799753986 1101 102-103 0.7431359260598107 1101 104-105 0.5780438739581264 1101 106-107 0.6980617298803757 1101 108-109 0.5972803977245214 1101 110-111 0.49139165517988914 1101 112-113 0.7977035574895446 1101 114-115 0.6368407484985887 1101 116-117 0.5152953545595906 1101 118-119 0.2785646310068053 1101 120-121 -0.02495975885707935 1101 122-123 0.20660703168077177 1101 124-125 0.2823705747248795 1102 1 0.32847364277208513 1102 2 0.4110752407900016 1102 3 0.3121225490955766 1102 4 0.32851658913030946 1102 5 0.3514423564777758 1102 6 0.2249032653527081 1102 7 0.3085174314458925 1102 8 0.2834707548159159 1102 9 0.3429158898349769 1102 10-11 0.3154556551096377 1102 12-13 0.32610686681780265 1102 14-15 0.4841980231491405 1102 16-17 0.409691361353687 1102 18-19 0.46140109555022235 1102 20-21 0.4579494041704777 1102 22-23 0.5228032923873371 1102 24-25 0.37777055119074276 1102 26-27 0.4847261614014755 1102 28-29 0.4626328768193986 1102 30-31 0.47405198876413124 1102 32-33 0.5793835697945511 1102 34-35 0.6593763272661732 1102 36-37 0.5843682091405995 1102 38-39 0.5396057243381875 1102 40-41 0.5792072595119109 1102 42-43 0.44096917980420614 1102 44-45 0.6050156730973839 1102 46-47 0.5370726324575799 1102 48-49 0.45682037917651286 1102 50-51 0.4286138866539133 1102 52-53 0.4683272718558982 1102 54-55 0.47309161290813506 1102 56-57 0.520305989258901 1102 58-59 0.37233222924892573 1102 60-61 0.3204129684320165 1102 62-63 0.37228668601162695 1102 64-65 0.2848369141142655 1102 66-67 0.36796100303849855 1102 68-69 0.48261746525735205 1102 70-71 0.43351212238469117 1102 72-73 0.42838208863595284 1102 74-75 0.455526968479127 1102 76-77 0.5049153881834165 1102 78-79 0.4390579744344265 1102 80-81 0.4944727591490583 1102 82-83 0.43410111789429706 1102 84-85 0.5011715170768518 1102 86-87 0.49597335095392125 1102 88-89 0.48809985452884774 1102 90-91 0.6229195115591324 1102 92-93 0.7002243362631937 1102 94-95 0.6443082383616243 1102 96-97 0.6489943144781236 1102 98-99 0.6819854210848568 1102 100-101 0.8419632025509145 1102 102-103 0.7595164242050174 1102 104-105 0.6315891839740253 1102 106-107 0.5792964940563152 1102 108-109 0.6828818815612969 1102 110-111 0.7980636212636192 1102 112-113 0.8044073200756579 1102 114-115 0.6671269170200489 1102 116-117 0.5115115707758093 1102 118-119 0.42859112809212263 1102 120-121 0.3347699708726495 1102 122-123 0.40158318430398054 1102 124-125 0.36506797801051505 1103 1 0.35634973396835434 1103 2 0.2470901122274647 1103 3 0.2831435838956331 1103 4 0.17918381136768602 1103 5 0.2933967914119293 1103 6 0.16638315030326822 1103 7 0.22195422328958614 1103 8 0.20324204487261 1103 9 0.3490585838734219 1103 10-11 0.33202150057334734 1103 12-13 0.3326484639347811 1103 14-15 0.35291219937914065 1103 16-17 0.22102963364451966 1103 18-19 0.25272890128566416 1103 20-21 0.2761690645944128 1103 22-23 0.32206699584484966 1103 24-25 0.3801263528949903 1103 26-27 0.39987224260243437 1103 28-29 0.329135104476066 1103 30-31 0.40613429590379013 1103 32-33 0.28546351410519577 1103 34-35 0.31675578619651645 1103 36-37 0.46271847828650436 1103 38-39 0.3911662414488575 1103 40-41 0.49525159603894764 1103 42-43 0.4164943057377002 1103 44-45 0.4083163583839351 1103 46-47 0.4396390053827659 1103 48-49 0.396665381561661 1103 50-51 0.3890563425079492 1103 52-53 0.43613310650582093 1103 54-55 0.5790237357256345 1103 56-57 0.4481334680178293 1103 58-59 0.39926542104400653 1103 60-61 0.4749356199263062 1103 62-63 0.20183436712396485 1103 64-65 0.22847128906001046 1103 66-67 0.4052350854167379 1103 68-69 0.4902974862896734 1103 70-71 0.5117024996023218 1103 72-73 0.5696896805262774 1103 74-75 0.564101359149511 1103 76-77 0.5203134603861166 1103 78-79 0.42402904259353136 1103 80-81 0.40084090576681675 1103 82-83 0.40887804219861223 1103 84-85 0.542782275306859 1103 86-87 0.5609850452213756 1103 88-89 0.4089194536085188 1103 90-91 0.30994346310542653 1103 92-93 0.5318744226164114 1103 94-95 0.5235216262930109 1103 96-97 0.3624289567922787 1103 98-99 0.4958303755189668 1103 100-101 0.5179278246016139 1103 102-103 0.6536281187204942 1103 104-105 0.5525125391912233 1103 106-107 0.44155050726930867 1103 108-109 0.3816009273024612 1103 110-111 0.34224224055465413 1103 112-113 0.3814472856170461 1103 114-115 0.22286132445676188 1103 116-117 0.35210411339643244 1103 118-119 0.36109052663722707 1103 120-121 0.04553299598824623 1103 122-123 0.15991984527439485 1103 124-125 -0.1152534923999724 1104 1 0.15794585811482875 1104 2 0.21577414602097988 1104 3 0.2606452367255656 1104 4 0.16734693588261962 1104 5 0.20388474861866257 1104 6 0.23167293777416376 1104 7 0.2039740467960982 1104 8 0.2782666739984805 1104 9 0.31821847589724683 1104 10-11 0.413399268246863 1104 12-13 0.3465170789808951 1104 14-15 0.44756134212877186 1104 16-17 0.5147857255967381 1104 18-19 0.30882581852918634 1104 20-21 0.37178873862760753 1104 22-23 0.4709658060686195 1104 24-25 0.39457835811087705 1104 26-27 0.514270677836322 1104 28-29 0.6461857764526968 1104 30-31 0.7316819509758261 1104 32-33 0.7772555475955798 1104 34-35 0.7103114480209811 1104 36-37 0.6426800385579625 1104 38-39 0.5415493321493656 1104 40-41 0.7939055522474447 1104 42-43 0.6817604440214211 1104 44-45 0.48157853510215887 1104 46-47 0.5856058390309826 1104 48-49 0.6090211019211296 1104 50-51 0.615595695539362 1104 52-53 0.5296384648106027 1104 54-55 0.5521796870893212 1104 56-57 0.47816976494708996 1104 58-59 0.5592408049839861 1104 60-61 0.5373259647863406 1104 62-63 0.3943857877737571 1104 64-65 0.2454973972400154 1104 66-67 0.2346882534932071 1104 68-69 0.3040394932563828 1104 70-71 0.2896202997314141 1104 72-73 0.1312806567281406 1104 74-75 0.22212692640335518 1104 76-77 0.2131633050331665 1104 78-79 0.22818045488117633 1104 80-81 0.17499247484465386 1104 82-83 0.2204926675876493 1104 84-85 0.2445662863657958 1104 86-87 0.23844684721104414 1104 88-89 0.29888736388271653 1104 90-91 0.31747069886822743 1104 92-93 0.3828960413706106 1104 94-95 0.29780372320502835 1104 96-97 0.1344254446919919 1104 98-99 0.2385942468778417 1104 100-101 0.21954746727676877 1104 102-103 0.3456946657039772 1104 104-105 0.24073027895608945 1104 106-107 0.3202483385996473 1104 108-109 0.3192035268838289 1104 110-111 0.18571597950421292 1104 112-113 0.17635356428025162 1104 114-115 0.29213750253905957 1104 116-117 0.1985345233766509 1104 118-119 0.1297597960210659 1104 120-121 0.0467691559874126 1104 122-123 0.20596598862766768 1104 124-125 0.22777326384616003 1105 1 0.2462796841075381 1105 2 0.3642813881137954 1105 3 0.2690647854495758 1105 4 0.26750705017959575 1105 5 0.31718162515822 1105 6 0.40124136816139355 1105 7 0.3347627944559619 1105 8 0.2814172307035747 1105 9 0.5064956460617935 1105 10-11 0.5044328676162593 1105 12-13 0.42504698340497526 1105 14-15 0.46987634853334015 1105 16-17 0.5194290402090189 1105 18-19 0.5387659604150912 1105 20-21 0.4230129971752419 1105 22-23 0.4394368376973503 1105 24-25 0.4049168151017142 1105 26-27 0.5865080903892803 1105 28-29 0.40531968127090323 1105 30-31 0.3927655552718079 1105 32-33 0.5040722290420305 1105 34-35 0.4848532748019423 1105 36-37 0.5297908376514684 1105 38-39 0.49044700679711184 1105 40-41 0.4408990984096306 1105 42-43 0.3706127940066466 1105 44-45 0.5342684552913468 1105 46-47 0.48502573261656323 1105 48-49 0.4640474591977082 1105 50-51 0.5119869656152289 1105 52-53 0.5083444949613565 1105 54-55 0.5639037485732104 1105 56-57 0.6244859044682229 1105 58-59 0.666814211230907 1105 60-61 0.5505600272555498 1105 62-63 0.60087041680125 1105 64-65 0.4305907561916378 1105 66-67 0.4663711779192603 1105 68-69 0.5024174122631813 1105 70-71 0.40454418385792934 1105 72-73 0.4473142560975347 1105 74-75 0.3912384152199877 1105 76-77 0.40030489533763003 1105 78-79 0.4063605711487881 1105 80-81 0.42303661712468 1105 82-83 0.45866391598650225 1105 84-85 0.4434873623338724 1105 86-87 0.46304409817172854 1105 88-89 0.5946578831457003 1105 90-91 0.6687528448924667 1105 92-93 0.6175004358922358 1105 94-95 0.6245612855264397 1105 96-97 0.6502065561315433 1105 98-99 0.525540005080618 1105 100-101 0.5869949990532959 1105 102-103 0.7042964983968538 1105 104-105 0.5614182777737078 1105 106-107 0.6388486933143973 1105 108-109 0.7324314311108466 1105 110-111 0.7428238226414656 1105 112-113 0.6403771133983938 1105 114-115 0.6320182789702358 1105 116-117 0.5672481897477226 1105 118-119 0.6067575297879415 1105 120-121 0.5301197323988802 1105 122-123 0.4912625695716031 1105 124-125 0.5438438126687046 1106 1 0.24854313982539722 1106 2 0.3537574469937219 1106 3 0.423798491012338 1106 4 -0.2655259616828687 1106 5 0.3591298138296679 1106 6 0.3883966329518742 1106 7 0.33411468286233514 1106 8 0.4371449580361144 1106 9 0.6438196360604778 1106 10-11 0.5871194993159179 1106 12-13 0.5493387135198375 1106 14-15 0.5946291355541931 1106 16-17 0.6323729426021885 1106 18-19 0.5530298203214699 1106 20-21 0.6672993694422757 1106 22-23 0.6626088246129171 1106 24-25 0.6235494359352813 1106 26-27 0.6308572058743991 1106 28-29 0.5817733499867117 1106 30-31 0.6720713379875818 1106 32-33 0.7001297460085496 1106 34-35 0.6532574821224628 1106 36-37 0.6877018879776386 1106 38-39 0.7061142468474984 1106 40-41 0.6857388799598354 1106 42-43 0.6606084231307747 1106 44-45 0.5739447284851806 1106 46-47 0.6835769987606071 1106 48-49 0.7143649351813934 1106 50-51 0.6773719786808527 1106 52-53 0.7697419062748736 1106 54-55 0.8385942623626192 1106 56-57 0.7847905968149647 1106 58-59 0.8085234116896132 1106 60-61 0.8653389925329336 1106 62-63 0.8132731295642017 1106 64-65 0.8558682833098672 1106 66-67 0.8746382431346085 1106 68-69 0.855346604074132 1106 70-71 0.7792050884882364 1106 72-73 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2216 82-83 -1.9588488425415669 2216 84-85 -1.8499856433067414 2216 86-87 -1.8625703256800037 2216 88-89 -1.9010243623552405 2216 90-91 -2.0328688311752217 2216 92-93 -1.7206274179890428 2216 94-95 -1.7123007880701238 2216 96-97 -1.7684981175187389 2216 98-99 -1.6441719207856202 2216 100-101 -1.7301887237508922 2216 102-103 -1.9195149439883181 2216 104-105 -2.290324268559889 2216 106-107 -1.8789796709080058 2216 108-109 -2.1801390765442683 2216 110-111 -2.281246147812464 2216 112-113 -2.415498524528008 2216 114-115 -2.2375761341374734 2216 116-117 -2.2438785897311924 2216 118-119 -2.259224205371879 2216 120-121 -1.8362178985439073 2216 122-123 -1.4003638070064213 2216 124-125 -1.2726079735252256 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 44104.0 3 7395.0 4 4399.0 5 8650.0 6 17958.0 7 19723.0 8 13839.0 9 12351.0 10 11769.0 11 10198.0 12 9164.0 13 7808.0 14 7174.0 15 7008.0 16 6408.0 17 6194.0 18 5990.0 19 6538.0 20 7181.0 21 8125.0 22 8994.0 23 10439.0 24 11710.0 25 13732.0 26 15314.0 27 16656.0 28 19292.0 29 23687.0 30 29553.0 31 38532.0 32 52621.0 33 72520.0 34 108902.0 35 164731.0 36 275415.0 37 277763.0 38 68537.0 39 85.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.91607884870528 19.449137264099754 11.698131701562342 15.93665218563262 2 18.01375605069756 24.309767746906566 36.8187961602761 20.857680042119778 3 27.738626083138623 24.778897795899418 26.255475993718953 21.227000127243006 4 13.978298398075257 21.5756769776894 34.25242463206632 30.19359999216902 5 15.390202808984837 37.11201683433421 27.895218853335756 19.602561503345196 6 30.596333065121055 39.1321247236027 12.267530911406759 18.004011299869482 7 29.428439801936868 39.794037273507065 14.087425451937252 16.690097472618813 8 33.81196891898032 30.849484954088034 13.35554071956126 21.98300540737038 9 35.44533607744088 16.293860921564338 16.814602865234164 31.44620013576062 10-11 29.35246658590005 28.19549529207059 19.283390855662415 23.16864726636695 12-13 30.464417365335184 28.943087498488246 20.605623789287215 19.986871346889355 14-15 31.748166148068556 24.513879810606245 22.627771924955557 21.110182116369643 16-17 27.91072655700024 26.454865186628908 23.626507295909917 22.007900960460944 18-19 27.0896963841676 26.5375309603421 24.178707673550935 22.194064981939363 20-21 33.876888467268195 24.40852901061827 21.102736953663126 20.611845568450406 22-23 31.012213562220236 26.230601506229817 20.882213331525058 21.874971600024885 24-25 24.771139892859566 28.54667627663568 23.28556777929322 23.396616051211534 26-27 23.24090962157824 28.015066682232963 24.21134461330457 24.53267908288423 28-29 23.858561297142504 27.848440654835482 24.569823762675274 23.723174285346744 30-31 24.30510067048409 28.03883229089404 23.58389859478671 24.07216844383516 32-33 22.462020931742888 28.296966218535445 24.82101898761167 24.419993862109994 34-35 22.675798467484913 28.66971370727857 24.688963472563703 23.965524352672812 36-37 22.56842034619657 28.750457021137972 23.611512109050313 25.06961052361515 38-39 22.38330493918386 27.843195785408742 24.678267605013495 25.095231670393908 40-41 21.593768154137937 27.603587379994813 26.126299320707545 24.6763451451597 42-43 21.332453429283888 27.537175130500067 26.115288868817633 25.015082571398413 44-45 21.053312258512825 27.401694141530797 26.55633611309377 24.988657486862607 46-47 20.163912422516127 27.647734049001055 26.855331051082203 25.33302247740061 48-49 19.621429205590655 27.944282219902846 26.745995516124545 25.688293058381962 50-51 19.72331220022692 27.795802320517147 26.054925104354766 26.42596037490117 52-53 19.974952095795825 27.478802258575747 25.835868067522384 26.710377578106048 54-55 19.277483660838936 27.000214616427314 26.021577689398995 27.700724033334755 56-57 19.878891215663327 26.7117434065306 26.156533313479436 27.252832064326633 58-59 20.722963747999767 25.909970156432305 25.979353480246548 27.387712615321373 60-61 20.75684125055009 26.00617214690255 25.291366369594087 27.945620232953278 62-63 20.986026163629994 25.490069970547914 25.457493014479965 28.066410851342123 64-65 21.071376389353976 24.947026455662662 25.679428088457257 28.3021690665261 66-67 21.74456956383139 25.313221797804335 24.882729702975833 28.059478935388437 68-69 21.67541737744344 25.565102491680413 25.075610335859505 27.68386979501664 70-71 21.57527134715305 25.510163413111226 25.411749158671746 27.50281608106398 72-73 22.64650387952941 24.683824819620583 25.459276301210032 27.210394999639973 74-75 22.853057871462777 24.094873542726305 26.013808050617865 27.038260535193047 76-77 22.340152965475635 24.008400133244457 26.633336910287543 27.018109990992368 78-79 23.259819873731246 24.17869733879272 26.74395518584828 25.81752760162775 80-81 23.993448172084054 24.074681889949286 26.395750120330064 25.536119817636592 82-83 23.70100216119517 23.8573855865095 26.592382791950975 25.849229460344358 84-85 23.75749210584461 23.50472530576742 26.992487616874055 25.745294971513914 86-87 24.077578613274422 23.069238405503278 27.088722193481633 25.76446078774066 88-89 24.2646421309646 22.735822143662233 27.081719326410237 25.917816398962934 90-91 25.213716590671165 22.871249233199865 26.302598993678593 25.612435182450373 92-93 25.708501528923698 22.99346025480081 26.167266035520253 25.130772180755244 94-95 25.016561691470457 23.140047760597124 26.390963591546562 25.452426956385853 96-97 24.856234700194598 23.453485270763952 25.785015690001778 25.905264339039668 98-99 24.366992924079 24.05117537608854 25.600201899519337 25.981629800313126 100-101 24.48470544809863 25.237164125264606 25.178301282678667 25.099829143958104 102-103 24.510349475238367 26.257725667076095 25.197226904091625 24.034697953593916 104-105 23.552902932643573 27.386591632003913 25.37729385857597 23.68321157677654 106-107 22.960468779164998 28.326583657184962 25.037418582686854 23.675528980963186 108-109 22.48627887971623 29.55240241069475 24.869045530141022 23.092273179447993 110-111 22.644479848775813 31.135495669571796 23.845667719242563 22.37435676240983 112-113 22.87976728814276 32.02587877773144 23.480701896656164 21.61365203746963 114-115 22.45667204988919 32.97346141208219 23.47004621181932 21.099820326209304 116-117 22.352300904814467 33.548462416608935 22.978288787025704 21.12094789155089 118-119 22.356953590261565 33.659102237846625 23.00502837753933 20.978915794352478 120-121 22.699595157550196 33.80462419421252 22.624722723818593 20.87105792441869 122-123 23.527663977404607 34.01617052811141 22.189068639102896 20.267096855381087 124-125 24.00955866508056 35.29757827295805 21.33124033139976 19.361622730561624 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 24.0 1 36.0 2 41.0 3 37.5 4 39.5 5 35.5 6 30.5 7 31.0 8 33.0 9 39.5 10 46.0 11 53.0 12 77.5 13 97.5 14 131.5 15 178.0 16 226.0 17 321.5 18 440.5 19 583.5 20 789.0 21 1100.0 22 1512.0 23 1987.0 24 2569.0 25 3442.5 26 4758.0 27 6646.5 28 8070.0 29 8979.0 30 10429.0 31 12351.5 32 15097.0 33 17488.0 34 20743.0 35 23679.0 36 25229.0 37 26320.5 38 25621.5 39 25570.0 40 25491.5 41 25095.5 42 25292.0 43 26480.5 44 28115.5 45 29610.5 46 32385.5 47 35624.0 48 40963.5 49 49160.5 50 59473.0 51 72005.0 52 85102.5 53 94242.0 54 95822.5 55 89465.5 56 79297.0 57 67071.0 58 52490.0 59 38468.5 60 27691.0 61 20525.5 62 15336.0 63 12102.5 64 9991.0 65 8346.5 66 7130.0 67 5958.0 68 4915.0 69 4289.0 70 3937.5 71 3365.0 72 2742.0 73 2109.5 74 1412.5 75 1026.0 76 745.5 77 592.5 78 486.5 79 333.5 80 208.5 81 179.0 82 198.5 83 119.5 84 40.5 85 25.0 86 18.0 87 21.0 88 22.0 89 19.0 90 14.5 91 8.0 92 6.0 93 5.5 94 2.0 95 0.5 96 0.5 97 0.5 98 1.5 99 1.5 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15058103727544794 2 0.01761672302386856 3 0.008738453880887185 4 0.01691764671339759 5 0.0034254739213077762 6 0.0 7 4.194457862825848E-4 8 2.796305241883899E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0012583373588477545 28-29 0.00803937757041621 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 1.3981526209419496E-4 52-53 0.0 54-55 0.0 56-57 6.64122494947426E-4 58-59 0.0 60-61 3.495381552354874E-5 62-63 0.0 64-65 0.001957413669318729 66-67 2.097228931412924E-4 68-69 0.00692085547366265 70-71 0.01957413669318729 72-73 1.3981526209419496E-4 74-75 0.00936762256031106 76-77 3.844919707590361E-4 78-79 0.0012583373588477545 80-81 0.0012932911743713032 82-83 3.495381552354874E-5 84-85 0.020063490110516974 86-87 0.002726397610836801 88-89 0.018944968013763417 90-91 1.048614465706462E-4 92-93 0.014296110549131432 94-95 0.014016480024943044 96-97 0.006256732978715223 98-99 0.0031108895815958374 100-101 0.0010486144657064621 102-103 0.0 104-105 6.291686794238772E-4 106-107 0.0013981526209419493 108-109 0.0 110-111 0.0 112-113 0.001433106436465498 114-115 0.006221779163191675 116-117 0.0 118-119 0.07490602666696494 120-121 0.0018175984072245344 122-123 0.0055227028527207005 124-125 0.24338341749046985 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1430459.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.5038178923745 #Duplication Level Percentage of deduplicated Percentage of total 1 79.06429767416562 39.13984593850384 2 9.398277555515627 9.305012412204723 3 3.516980563386091 5.2231189602265715 4 1.9847096124766557 3.9300281290115806 5 1.2601354835302643 3.119075874820074 6 0.8601026326243831 2.5547018456513615 7 0.6495540464808639 2.250878365977055 8 0.49540832675080904 1.961968287187041 9 0.3535942571813774 1.5753839143766706 >10 2.2193021445307015 19.820338152069805 >50 0.13280836992111394 4.463345373719997 >100 0.06186330636687838 5.507675261084792 >500 0.0025423089168166674 0.9042609730739519 >1k 4.2371815280277793E-4 0.24436651209258964 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 6.990763104709746E-5 0.0 0.0 0.0 0.0 8 6.990763104709746E-5 0.0 0.0 0.0 0.0 9 6.990763104709746E-5 0.0 0.0 0.0 0.0 10-11 1.3981526209419493E-4 0.0 0.0 3.495381552354873E-5 0.0 12-13 5.592610483767797E-4 0.0 0.0 1.3981526209419493E-4 0.0 14-15 5.942148639003285E-4 0.0 0.0 2.0972289314129242E-4 0.0 16-17 6.291686794238772E-4 0.0 0.0 6.990763104709747E-4 0.0 18-19 7.340301259945235E-4 0.0 0.0 0.0011534759122771082 0.0 20-21 8.738453880887183E-4 0.0 0.0 0.003075935766072289 0.0 22-23 0.001048614465706462 0.0 0.0 0.009891929793164292 0.0 24-25 0.001328244989894852 0.0 0.0 0.022789887721353777 0.0 26-27 0.0020622751158893755 0.0 0.0 0.058337918108802844 0.0 28-29 0.002376859455601314 0.0 0.0 0.20399046739543042 0.0 30-31 0.002621536164266155 0.0 0.0 0.570166638820127 0.0 32-33 0.002901166688454545 0.0 0.0 1.138935125019312 0.0 34-35 0.0029710743195016424 0.0 0.0 1.9102959259929855 0.0 36-37 0.0034604277368313245 0.0 0.0 3.071706354393939 0.0 38-39 0.0038798735231139097 0.0 0.0 4.809610062224783 0.0 40-41 0.0043342731249200435 0.0 0.0 6.815294950781532 0.0 42-43 0.004718765095679079 0.0 0.0 8.375528414306178 0.0 44-45 0.004893534173296823 0.0 0.0 9.876060760916602 0.0 46-47 0.0049284879888203716 0.0 0.0 11.622598061181762 0.0 48-49 0.00496344180434392 0.0 0.0 13.524994424866424 0.0 50-51 0.004998395619867469 0.0 0.0 15.565877805655386 0.0 52-53 0.005033349435391018 0.0 0.0 17.34051797360148 0.0 54-55 0.005068303250914567 0.0 0.0 18.934132330951115 0.0 56-57 0.005103257066438115 0.0 0.0 20.635858839715084 0.0 58-59 0.005103257066438115 0.0 0.0 22.456847767045403 0.0 60-61 0.005103257066438115 0.0 0.0 24.44851617557721 0.0 62-63 0.005103257066438115 0.0 0.0 26.350842631630826 0.0 64-65 0.005103257066438115 0.0 0.0 28.01960769235609 0.0 66-67 0.005103257066438115 0.0 0.0 29.776351506754125 0.0 68-69 0.005103257066438115 0.0 0.0 31.677489533079942 0.0 70-71 0.005103257066438115 0.0 0.0 33.76178555274915 0.0 72-73 0.005138210881961664 0.0 0.0 35.81839815052371 0.0 74-75 0.005173164697485213 0.0 0.0 37.616946728287914 0.0 76-77 0.005173164697485213 3.495381552354873E-5 0.0 39.42937197081496 0.0 78-79 0.005173164697485213 6.990763104709746E-5 0.0 41.327818553345466 0.0 80-81 0.005173164697485213 6.990763104709746E-5 0.0 43.251466836868445 0.0 82-83 0.005173164697485213 6.990763104709746E-5 0.0 45.22111434162042 0.0 84-85 0.005173164697485213 1.048614465706462E-4 0.0 47.11473729760867 0.0 86-87 0.005173164697485213 1.3981526209419493E-4 0.0 48.84620950338318 0.0 88-89 0.00524307232853231 1.3981526209419493E-4 0.0 50.53297577910307 0.0 90-91 0.00524307232853231 1.3981526209419493E-4 0.0 52.31929052143403 0.0 92-93 0.00524307232853231 1.3981526209419493E-4 0.0 54.01699734141279 0.0 94-95 0.00524307232853231 1.3981526209419493E-4 0.0 55.611310775072894 0.0 96-97 0.00524307232853231 1.747690776177437E-4 0.0 57.1094313084122 0.0 98-99 0.00524307232853231 2.0972289314129242E-4 0.0 58.52757751183361 0.0 100-101 0.00524307232853231 2.0972289314129242E-4 0.0 59.96994670941285 0.0 102-103 0.00524307232853231 2.0972289314129242E-4 0.0 61.39627210566678 0.0 104-105 0.00524307232853231 2.0972289314129242E-4 0.0 62.72458700319268 0.0 106-107 0.00524307232853231 2.0972289314129242E-4 0.0 63.93129757651216 0.0 108-109 0.00524307232853231 2.7963052418838986E-4 0.0 65.07257460717155 0.0 110-111 0.00524307232853231 2.7963052418838986E-4 0.0 66.23758527857143 0.0 112-113 0.00524307232853231 2.7963052418838986E-4 0.0 67.3856783032579 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACGAGT 65 0.0019414669 36.606125 7 GTGCTAG 280 0.0 36.18289 1 CTACGGG 175 4.456524E-10 33.996155 3 TAAGCGT 45 2.3954693E-4 33.051815 22-23 GTAAGCG 260 0.0 32.03484 3 TAGGCCG 75 0.0039156484 31.72531 5 ACGAGTC 75 0.0039156484 31.72531 8 AGAGTTG 2035 0.0 30.400173 5 TCTAGGG 475 0.0 30.061861 2 GTACAGG 860 0.0 29.79768 1 GCGTCAG 80 0.0053208186 29.797678 1 TAAGCGG 260 1.8189894E-12 29.746635 4 GGTATCA 31545 0.0 29.339542 1 ACAACAC 1030 0.0 28.87619 5 TCTACGG 165 3.410023E-7 28.847237 2 GAGTTAA 810 0.0 28.694061 1 GAGTAAG 1965 0.0 28.50872 1 CGCAGAA 735 0.0 28.33211 2 GTGCAAG 360 0.0 28.142254 1 AGTTTCG 625 0.0 27.601019 7 >>END_MODULE