FastQCFastQC Report
Thu 26 May 2016
SRR935995_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935995_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1430459
Sequences flagged as poor quality0
Sequence length125
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT69720.4873960036603636No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT37840.2645304758822168TruSeq Adapter, Index 3 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT21650.15135002121696603RNA PCR Primer, Index 3 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA320550.036.673851
AGTTGCG1902.910383E-1134.444277
AGTCGTG1902.910383E-1134.444277
TATTCGA350.002790906733.99694424-25
GTATACG3650.032.731381
TGTACGG1659.258656E-932.5141332
GTACAGG8600.031.2565631
CTACGGG1352.2985823E-630.9074173
TCTACGG1551.9399522E-730.7660662
GTATCAA430000.030.6453251
TTGACGT603.7001875E-529.74732832-33
GCGCTCA1454.0706545E-628.7215569
CGCGTCC850.007214115527.9974828
GTATAGG8900.027.5182651
TACGGGT1954.702997E-827.5114
GTGCTAG3050.027.4192391
GTCATAG5250.027.3073231
GTAATAG3100.026.9769941
GTCCAGG6000.026.8806481
GTATGGG11950.026.4932441
Produced by FastQC (version 0.11.5)