FastQCFastQC Report
Thu 26 May 2016
SRR935994_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935994_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences728822
Sequences flagged as poor quality0
Sequence length125
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT17920.24587622217770597No Hit
ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA17370.23832979794792142No Hit
GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT15340.2104766321543532No Hit
GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA13460.18468158205981708No Hit
TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC10770.14777270719050742No Hit
ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC10550.14475413749859362No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATTA255.337672E-447.591686106-107
GTAGGGC705.46225E-542.483834
GGCATGA600.001310647139.6515738
TAGGGAG1555.133188E-934.535245
CTCTATG700.00279258733.9893952
CTTGGTA1250.033.30732316-17
GTCCTAG1659.309588E-932.4912381
GCAGAGA8250.031.7212583
CTTGTGT750.00391684731.7212563
TTGTGTT953.292484E-431.3038754
AGTAAGA2950.030.2447992
GTCTAAG1004.4077885E-429.7836341
GTAACAA1203.665797E-529.7836321
TAGAAGG1403.0998835E-629.7407232
TGTTAGG1004.445173E-429.7407232
TATTAGT1101.5825208E-1029.7407258-59
TACGTTA800.005370915429.738684
TATAAGG1852.8750947E-828.9369162
GTATACG2502.1827873E-1128.5922891
GTATTAG1055.864663E-428.3653641
Produced by FastQC (version 0.11.5)