##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935994_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 728822 Sequences flagged as poor quality 0 Sequence length 125 %GC 51 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.77846991446471 31.0 31.0 34.0 25.0 34.0 2 29.7719758733957 33.0 31.0 34.0 23.0 34.0 3 29.778808817516484 33.0 31.0 34.0 23.0 34.0 4 32.97150607418546 37.0 35.0 37.0 24.0 37.0 5 33.14565010386624 37.0 35.0 37.0 26.0 37.0 6 33.29326639426362 37.0 35.0 37.0 27.0 37.0 7 33.281304625820844 37.0 35.0 37.0 27.0 37.0 8 33.124658695813245 37.0 35.0 37.0 25.0 37.0 9 34.47905661464665 39.0 37.0 39.0 21.0 39.0 10-11 34.47808381195957 39.0 37.0 39.0 22.5 39.0 12-13 34.43091454429202 39.0 35.0 39.0 23.5 39.0 14-15 35.496621260060756 40.0 36.0 41.0 23.0 41.0 16-17 35.52156356421733 40.0 36.0 41.0 24.5 41.0 18-19 35.645072459393376 40.0 36.0 41.0 25.5 41.0 20-21 35.76893535046966 40.0 36.0 41.0 26.5 41.0 22-23 35.559617437453866 40.0 36.0 41.0 25.5 41.0 24-25 35.32305075862145 40.0 35.0 41.0 25.0 41.0 26-27 35.13712264448658 40.0 35.0 41.0 24.0 41.0 28-29 34.96702487027011 40.0 35.0 41.0 23.0 41.0 30-31 34.79824703425528 40.0 35.0 41.0 20.0 41.0 32-33 34.47396620848437 40.0 35.0 41.0 15.0 41.0 34-35 34.2932403247981 40.0 35.0 41.0 8.0 41.0 36-37 34.2004549807772 40.0 35.0 41.0 2.0 41.0 38-39 34.08692245843292 39.5 35.0 41.0 2.0 41.0 40-41 33.85820611891518 39.0 35.0 41.0 2.0 41.0 42-43 33.61219611921704 39.0 34.0 41.0 2.0 41.0 44-45 33.31319512857735 38.5 34.0 41.0 2.0 41.0 46-47 32.98427805417509 38.0 34.0 40.5 2.0 41.0 48-49 32.61610379489093 37.5 33.0 40.0 2.0 41.0 50-51 32.24509139405781 37.0 33.0 39.5 2.0 41.0 52-53 31.864184258982306 36.0 33.0 39.0 2.0 41.0 54-55 31.444456259553085 35.0 33.0 39.0 2.0 41.0 56-57 31.046414625244573 35.0 32.0 37.5 2.0 40.5 58-59 30.65432163134483 35.0 32.0 37.0 2.0 39.5 60-61 30.310234872163573 35.0 32.0 36.5 2.0 39.0 62-63 29.952915801114678 35.0 31.0 36.0 2.0 39.0 64-65 29.712622560789878 35.0 31.0 35.0 2.0 37.0 66-67 29.57444410294969 35.0 31.0 35.0 2.0 37.0 68-69 29.455797849131883 35.0 31.5 35.0 2.0 36.0 70-71 29.252816188314846 35.0 31.0 35.0 2.0 36.0 72-73 29.09061609007412 35.0 31.0 35.0 2.0 35.5 74-75 28.92291945083985 35.0 31.0 35.0 2.0 35.0 76-77 28.759280729725504 35.0 31.0 35.0 2.0 35.0 78-79 28.61283207696804 35.0 30.5 35.0 2.0 35.0 80-81 28.45389272003315 35.0 30.0 35.0 2.0 35.0 82-83 28.30178356306478 35.0 29.5 35.0 2.0 35.0 84-85 28.17075705727873 35.0 29.0 35.0 2.0 35.0 86-87 28.03994665364108 35.0 29.0 35.0 2.0 35.0 88-89 27.83138544116396 34.0 29.0 35.0 2.0 35.0 90-91 27.5698229471668 34.0 28.0 35.0 2.0 35.0 92-93 27.261801921456815 34.0 27.0 35.0 2.0 35.0 94-95 27.024527250823933 34.0 25.0 35.0 2.0 35.0 96-97 26.8081205013021 34.0 25.0 35.0 2.0 35.0 98-99 26.526335648484817 34.0 24.0 35.0 2.0 35.0 100-101 26.296873585045457 33.5 23.5 35.0 2.0 35.0 102-103 25.58437862742892 32.5 21.5 34.5 2.0 35.0 104-105 26.04457604188677 33.0 23.0 35.0 2.0 35.0 106-107 26.051507775561113 34.0 23.0 35.0 2.0 35.0 108-109 25.851338872866076 34.0 20.0 35.0 2.0 35.0 110-111 25.516251567598125 33.0 18.5 35.0 2.0 35.0 112-113 25.07270087895261 33.0 11.5 35.0 2.0 35.0 114-115 24.512144254701422 33.0 2.0 35.0 2.0 35.0 116-117 23.896118668207052 33.0 2.0 35.0 2.0 35.0 118-119 23.08652935833441 32.5 2.0 35.0 2.0 35.0 120-121 22.219338192315817 31.5 2.0 35.0 2.0 35.0 122-123 21.322030619273292 31.0 2.0 35.0 2.0 35.0 124-125 18.68246979372192 25.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.45978516840045813 1101 2 0.459550560127326 1101 3 0.33764975593764035 1101 4 0.6218423351022935 1101 5 0.5885569104923505 1101 6 0.5367599942819368 1101 7 0.4731714460494203 1101 8 0.39114759012554856 1101 9 0.5095271702764634 1101 10-11 0.6445123678003526 1101 12-13 0.4251505513446858 1101 14-15 0.6847527919898511 1101 16-17 0.6935080718896813 1101 18-19 0.7705791577278802 1101 20-21 0.776492706609595 1101 22-23 0.7077783850855255 1101 24-25 0.8248242873874929 1101 26-27 0.8762826059482265 1101 28-29 0.9552598539700412 1101 30-31 0.8522978355583817 1101 32-33 0.9391192310946792 1101 34-35 0.3631017287595242 1101 36-37 0.5880028342280426 1101 38-39 0.7669000908935715 1101 40-41 0.8067488601695416 1101 42-43 0.5456542216950169 1101 44-45 0.6666965409233043 1101 46-47 0.7221741812773104 1101 48-49 0.7490483025460506 1101 50-51 0.8587617540717787 1101 52-53 0.7792086027620648 1101 54-55 0.8921025663979414 1101 56-57 0.7032536019888482 1101 58-59 0.5854081989306508 1101 60-61 0.7126409234429261 1101 62-63 0.7116253245923971 1101 64-65 0.7400293464810161 1101 66-67 0.6599138427089066 1101 68-69 0.6146581778213225 1101 70-71 0.5376109968328926 1101 72-73 0.6490481636654444 1101 74-75 0.6833487354692629 1101 76-77 0.9147406892157548 1101 78-79 0.9562258933434045 1101 80-81 0.8657747277122034 1101 82-83 0.6794501477715684 1101 84-85 0.5408163218577329 1101 86-87 0.5213694204929631 1101 88-89 0.6374575393630231 1101 90-91 0.591364349485989 1101 92-93 0.2904559945122074 1101 94-95 0.24812630242449885 1101 96-97 0.3570642441665477 1101 98-99 0.14843061191326967 1101 100-101 0.4816736275181981 1101 102-103 0.5365772456369484 1101 104-105 0.1830822746996148 1101 106-107 0.4687189055338443 1101 108-109 0.6895779764932612 1101 110-111 0.41280164976154055 1101 112-113 0.2865583269106047 1101 114-115 0.3190492968962353 1101 116-117 0.47351289277217745 1101 118-119 0.4335181950036642 1101 120-121 -0.22874993017098433 1101 122-123 -0.17564955157006779 1101 124-125 -0.13785832230561113 1102 1 -0.14745999232984275 1102 2 -0.07950598723337876 1102 3 -0.17189375649930483 1102 4 -0.22175465443955034 1102 5 -0.08780276274241317 1102 6 -0.04003658760709072 1102 7 -0.08350290429410023 1102 8 0.08982850535112874 1102 9 9.031271192725399E-4 1102 10-11 0.07565826442757384 1102 12-13 0.020026278320173674 1102 14-15 0.11401313360804721 1102 16-17 0.1701082217053056 1102 18-19 0.08494441850672985 1102 20-21 -0.015192847756438255 1102 22-23 0.04941748374843513 1102 24-25 0.11085317672647221 1102 26-27 0.07952068097820586 1102 28-29 -0.044767472914152506 1102 30-31 -0.15378802229680133 1102 32-33 0.020487439962096232 1102 34-35 0.15043105629312237 1102 36-37 0.09893198043973683 1102 38-39 0.09179102447637888 1102 40-41 0.10179562129077624 1102 42-43 -0.05245893732494977 1102 44-45 -0.018017121554308346 1102 46-47 0.06028489695233219 1102 48-49 0.061474962011281775 1102 50-51 0.05510702443110915 1102 52-53 0.19935140984390998 1102 54-55 0.2258843821247254 1102 56-57 0.11588473084636064 1102 58-59 0.20570114312915422 1102 60-61 0.019426992518859265 1102 62-63 0.11854031226314987 1102 64-65 0.06887946334363093 1102 66-67 -0.17146642213682028 1102 68-69 0.024231910577253046 1102 70-71 -0.037086237364935215 1102 72-73 0.11225331783729331 1102 74-75 0.2716065340797691 1102 76-77 0.26777349112155946 1102 78-79 0.2376686885300181 1102 80-81 0.3438714263030995 1102 82-83 0.30773057947203597 1102 84-85 0.2682113625104918 1102 86-87 0.3410409191957413 1102 88-89 0.3528994793789053 1102 90-91 0.2756665012369979 1102 92-93 0.5070311964900327 1102 94-95 0.51025542034483 1102 96-97 0.45113141686280755 1102 98-99 0.221806510887383 1102 100-101 0.21126928608003226 1102 102-103 0.43939641560695364 1102 104-105 0.27564164637417576 1102 106-107 0.00684633883213337 1102 108-109 0.265811946060758 1102 110-111 0.28278676464697483 1102 112-113 0.39438699699152124 1102 114-115 0.11841209825550791 1102 116-117 -1.086266668828273E-4 1102 118-119 -0.15658014963013756 1102 120-121 -0.26926123224879106 1102 122-123 -0.2203981281608911 1102 124-125 -0.24025215736053696 1103 1 0.42302504378986683 1103 2 0.4699049600533023 1103 3 0.4546233224531697 1103 4 0.4297734085834719 1103 5 0.5475587919889122 1103 6 0.33823743174531273 1103 7 0.2864354827334594 1103 8 0.3997325607721507 1103 9 0.336381164087058 1103 10-11 0.6409113067596692 1103 12-13 0.503191018942843 1103 14-15 0.6102979633294154 1103 16-17 0.6913299568819156 1103 18-19 0.6635060672412578 1103 20-21 0.636272376165337 1103 22-23 0.7232518950533446 1103 24-25 0.7836714337843702 1103 26-27 0.5995617562931628 1103 28-29 0.6831897964969968 1103 30-31 0.6463294817136571 1103 32-33 0.630414996185209 1103 34-35 0.5661280594299782 1103 36-37 0.6895285942806808 1103 38-39 0.6361257985110598 1103 40-41 0.751685269698342 1103 42-43 0.5309070927765163 1103 44-45 0.6073872455077876 1103 46-47 0.5966917264897731 1103 48-49 0.5706402748273902 1103 50-51 0.6272687916914208 1103 52-53 0.6436580850934739 1103 54-55 0.7369747171644256 1103 56-57 0.7289894933349927 1103 58-59 0.6267450896290612 1103 60-61 0.7237725973579856 1103 62-63 0.5354915429899378 1103 64-65 0.7481681042820298 1103 66-67 0.832595129529178 1103 68-69 0.8266576020628058 1103 70-71 0.7839086639827819 1103 72-73 0.7461195063137289 1103 74-75 0.7351680295482019 1103 76-77 0.8171619593801509 1103 78-79 0.8600623984892479 1103 80-81 0.7693156425513514 1103 82-83 0.752680461457139 1103 84-85 0.7057094466842315 1103 86-87 0.7651481441597205 1103 88-89 0.6791856374064729 1103 90-91 0.6427955206404867 1103 92-93 0.77312669148845 1103 94-95 0.9504606947422367 1103 96-97 0.7772954728968955 1103 98-99 0.9425326856720453 1103 100-101 0.8392602740333182 1103 102-103 0.8354375522283526 1103 104-105 0.6982524833092612 1103 106-107 0.7228340366731274 1103 108-109 0.7124201672543933 1103 110-111 0.5797541394030254 1103 112-113 0.6086443205566958 1103 114-115 0.5542605282997499 1103 116-117 0.2511292525389983 1103 118-119 0.21382365545625248 1103 120-121 0.2658034971343639 1103 122-123 0.2949800814866954 1103 124-125 0.1857683394259837 1104 1 -0.0056878274972618215 1104 2 0.06099406896419168 1104 3 0.10907876799772609 1104 4 -0.08012758151554067 1104 5 -0.003926356525944641 1104 6 0.2392275307552083 1104 7 0.06267310649583635 1104 8 -0.08739615209913865 1104 9 -0.26064853888323825 1104 10-11 -0.1373065150225088 1104 12-13 0.02744844468541885 1104 14-15 -0.05702876934385159 1104 16-17 0.07994381826805608 1104 18-19 -0.09045432879834436 1104 20-21 0.024886237551470458 1104 22-23 0.04651922178602064 1104 24-25 -0.0361305464136521 1104 26-27 0.25005680579811695 1104 28-29 0.3411105885762069 1104 30-31 0.27306215498098396 1104 32-33 0.23932588727431892 1104 34-35 0.1874151881428432 1104 36-37 0.023687010122266372 1104 38-39 -0.09951776584537697 1104 40-41 -0.056730571885815095 1104 42-43 -0.004736471579917634 1104 44-45 -0.13023651686845739 1104 46-47 0.24520657797492618 1104 48-49 0.33747195799571017 1104 50-51 0.28370443525578537 1104 52-53 0.1382125405390262 1104 54-55 0.29341036072877813 1104 56-57 0.060177612146873116 1104 58-59 0.012388653985500042 1104 60-61 0.12575279537778528 1104 62-63 0.09192718655429388 1104 64-65 0.13084137160875997 1104 66-67 -0.006018731856958226 1104 68-69 -0.008490912788676752 1104 70-71 -0.10074480136375286 1104 72-73 -0.13506861249815572 1104 74-75 -0.0039408813428885026 1104 76-77 0.0701322564098561 1104 78-79 -0.06518512626322703 1104 80-81 -0.036624951508056114 1104 82-83 0.13188838224921895 1104 84-85 0.1834322189614639 1104 86-87 0.07950457980328807 1104 88-89 0.011363855228257336 1104 90-91 0.08982522361078438 1104 92-93 0.26124550336963637 1104 94-95 0.3153121798907499 1104 96-97 0.2857113144810519 1104 98-99 0.18956238864234365 1104 100-101 0.14619096710262625 1104 102-103 0.24484349282241524 1104 104-105 0.0730049585567869 1104 106-107 0.12035878914837639 1104 108-109 0.2960835335910268 1104 110-111 0.09955611960104704 1104 112-113 0.42399085521016033 1104 114-115 0.3968347857254919 1104 116-117 0.06449558917265819 1104 118-119 -0.010928819791271138 1104 120-121 0.1598629030749592 1104 122-123 -0.02581199772122389 1104 124-125 0.018441606752716666 1105 1 0.2969586930789667 1105 2 0.27032258475415816 1105 3 0.18839461427429782 1105 4 0.26939613862899137 1105 5 0.1986497778647447 1105 6 0.2691041203098976 1105 7 0.06015328105358009 1105 8 0.22369201832928098 1105 9 0.16563835520901193 1105 10-11 0.16817656883090137 1105 12-13 0.15721710875561712 1105 14-15 0.21266763157586155 1105 16-17 0.311983609924944 1105 18-19 0.3383570831485372 1105 20-21 0.1274905264163806 1105 22-23 0.12648965958809555 1105 24-25 0.22970634533239576 1105 26-27 0.19812587810819338 1105 28-29 0.15740264432206885 1105 30-31 0.14684095466189717 1105 32-33 0.12708338340307535 1105 34-35 0.09625100063404801 1105 36-37 0.08678822545707732 1105 38-39 0.24658049326963294 1105 40-41 0.10830157113891659 1105 42-43 0.1035385976331682 1105 44-45 0.11095347890837814 1105 46-47 0.2757119507870911 1105 48-49 0.2408669183448282 1105 50-51 0.27310427571376295 1105 52-53 0.3317980128303297 1105 54-55 0.2905198347093503 1105 56-57 0.3606374685590552 1105 58-59 0.37401410563019155 1105 60-61 0.30511556577804555 1105 62-63 0.2534353278897541 1105 64-65 0.12380979167351569 1105 66-67 0.1630911175166041 1105 68-69 0.3274036956295099 1105 70-71 0.3254294748316404 1105 72-73 0.264342489749513 1105 74-75 0.3141554069589283 1105 76-77 0.2539457799423026 1105 78-79 0.338226257529179 1105 80-81 0.31685916467607456 1105 82-83 0.2021901043645613 1105 84-85 0.29449927353421756 1105 86-87 0.39405105814780583 1105 88-89 0.5160069972675778 1105 90-91 0.5533182553784144 1105 92-93 0.5432594867584939 1105 94-95 0.29588090026989633 1105 96-97 0.41234075467625786 1105 98-99 0.5371059107670675 1105 100-101 0.5712461029479634 1105 102-103 0.4210201152239925 1105 104-105 0.21601795653904787 1105 106-107 0.4189534116437983 1105 108-109 0.42924715801034097 1105 110-111 0.23767258527604795 1105 112-113 0.2628158751529135 1105 114-115 0.4090327116792274 1105 116-117 0.32052533906707836 1105 118-119 0.10017108166806565 1105 120-121 0.16895914445264992 1105 122-123 0.14627049488822408 1105 124-125 0.1919670161656981 1106 1 0.1509951092716264 1106 2 0.30166870705475546 1106 3 0.346809042428351 1106 4 -0.2676527044674941 1106 5 0.2895847438825072 1106 6 0.298362097158531 1106 7 0.26639732363223345 1106 8 0.46727972708840326 1106 9 0.510024524681306 1106 10-11 0.5933305196818992 1106 12-13 0.6089774020713321 1106 14-15 0.7502418361077119 1106 16-17 0.5487478268260659 1106 18-19 0.5104783463213636 1106 20-21 0.671692265207561 1106 22-23 0.5228888278766561 1106 24-25 0.4990012969858455 1106 26-27 0.43091242176195976 1106 28-29 0.5655175942908173 1106 30-31 0.6527183525932614 1106 32-33 0.7395018637119932 1106 34-35 0.7138570371921062 1106 36-37 0.7098575775999691 1106 38-39 0.80897170798508 1106 40-41 0.8358154366907158 1106 42-43 0.8352402810064703 1106 44-45 0.7164815225687917 1106 46-47 0.8042920562140452 1106 48-49 0.7089160762148268 1106 50-51 0.7183514172598748 1106 52-53 0.7159950521858853 1106 54-55 0.7560042534201976 1106 56-57 0.7459716826671148 1106 58-59 0.860551552595286 1106 60-61 0.7566422173414935 1106 62-63 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-2.1634236923003023 2216 74-75 -2.0532252983729258 2216 76-77 -2.0892699179580454 2216 78-79 -2.426420615830782 2216 80-81 -2.4825411069443923 2216 82-83 -2.5218991858567037 2216 84-85 -2.349423919568707 2216 86-87 -2.5243378078167105 2216 88-89 -2.518655059596444 2216 90-91 -2.6210148122102126 2216 92-93 -2.434748068937722 2216 94-95 -2.3061586569647012 2216 96-97 -2.576122586576002 2216 98-99 -2.437188697286203 2216 100-101 -2.288514806940544 2216 102-103 -2.4007303545031498 2216 104-105 -2.886542006283399 2216 106-107 -2.6710378948987312 2216 108-109 -3.147339798598491 2216 110-111 -3.142505931067248 2216 112-113 -3.400830249047349 2216 114-115 -3.1383806842576867 2216 116-117 -3.016292054257786 2216 118-119 -3.1156738462803943 2216 120-121 -2.4099558159059846 2216 122-123 -2.04666977665212 2216 124-125 -1.9010758141356128 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 38602.0 3 4334.0 4 2686.0 5 4445.0 6 10634.0 7 9119.0 8 6070.0 9 5798.0 10 4460.0 11 3481.0 12 2926.0 13 2722.0 14 2676.0 15 2726.0 16 2597.0 17 2570.0 18 2952.0 19 3420.0 20 3836.0 21 4597.0 22 5262.0 23 6074.0 24 6723.0 25 7560.0 26 8409.0 27 9570.0 28 11265.0 29 13987.0 30 17690.0 31 23639.0 32 31878.0 33 44183.0 34 65086.0 35 102361.0 36 185897.0 37 65305.0 38 3282.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 56.35383029886637 18.107179663345928 11.119340432840948 14.419649604946756 2 19.42846870287042 23.505404763767153 34.56441460171344 22.501711931648984 3 32.64551433428882 23.042165161633807 23.802480045336228 20.509840458741152 4 16.634532652503914 21.768473170042224 28.68691498369056 32.9100791937633 5 16.817210733794276 33.74844436272728 25.638411582860066 23.795933320618385 6 32.63018953873511 35.51649099505777 9.001100405860415 22.852219060346695 7 28.785296781098214 40.047885623336356 11.759144919184434 19.407672676381 8 36.446223273542536 27.54030836188409 11.499425783356362 24.514042581217012 9 36.86057775423903 17.155766428565546 15.24185603617893 30.74179978101649 10-11 30.07455867139027 27.610994179648802 15.01072964317762 27.303717505783304 12-13 32.6740274031245 28.90040366509244 17.244059592054025 21.181509339729043 14-15 36.45087552241837 22.014703178553884 20.72186350027853 20.812557798749214 16-17 30.359539091849584 23.64850402430223 22.454728315006957 23.537228568841225 18-19 29.667806405404885 24.334679798359545 22.165151436153135 23.832362360082435 20-21 37.500514528924754 22.468449086333838 18.162390816962166 21.86864556777924 22-23 32.8554846039225 25.959630746602052 17.038453833720716 24.146430815754737 24-25 27.38137707149345 27.88918281830131 17.703087996794828 27.026352113410407 26-27 25.19744460848614 26.511089279539853 20.44848328512703 27.842982826846978 28-29 26.9390549883467 24.3568729773102 23.568414170029097 25.135657864314005 30-31 28.768272637214576 26.10335582625113 20.14943292052106 24.978938616013238 32-33 24.805233651014927 25.472886383780953 22.706367261141953 27.01551270406217 34-35 23.046093559195523 26.20708485748235 22.640164539489753 28.106657043832378 36-37 24.755495854954983 28.559236686049545 19.110427511793002 27.574839947202474 38-39 26.43827985434029 24.387984994964476 21.065225802733725 28.1085093479615 40-41 23.964424784103663 25.146812253197627 22.640438954916288 28.248324007782422 42-43 24.077689751407068 25.291360579126316 24.124408977775094 26.506540691691523 44-45 25.019963722280615 23.618112515813188 25.2687899102936 26.093133851612606 46-47 22.388662801068023 24.401225539294916 25.22474623433431 27.98536542530275 48-49 21.69391154493141 25.130484535318637 26.008476692525743 27.167127227224206 50-51 22.551765111049214 25.201455501419417 24.737692794252634 27.509086593278738 52-53 22.131947169542084 25.116489348565217 23.632382117993146 29.119181363899553 54-55 20.599062596902947 24.973313099769218 23.24415289329905 31.183471410028783 56-57 20.972577440670896 23.725604267743847 24.658075563653924 30.64374272793133 58-59 23.04979816745378 21.849573695634874 25.01557307545601 30.085055061455336 60-61 23.391873044359972 22.51957440882301 24.10110020080363 29.987452346013395 62-63 22.515648539698308 21.80793115465779 24.871779391950298 30.804640913693603 64-65 22.30005124824113 21.677937392075115 24.648071399022097 31.373939960661655 66-67 23.762572540152206 23.171754910845674 22.69749547385124 30.368177075150875 68-69 23.94834085049336 22.289397168132364 23.34850029570088 30.413761685673396 70-71 23.572821982627598 22.25366396568062 24.123334584446354 30.050179467245425 72-73 24.760743721709446 21.61065611446432 24.492776690024026 29.13582347380221 74-75 25.494986669081737 20.12124754890862 25.571829841771677 28.811935940237966 76-77 24.63104744655745 20.15579978595538 26.333868444883514 28.87928432260366 78-79 24.852156660428204 21.062153202746387 26.735083944830766 27.350606191994643 80-81 26.27095795134675 21.62736404044673 25.676839178359806 26.424838829846713 82-83 25.320174198912763 21.560957270773933 26.175732895000426 26.943135635312878 84-85 24.904948053364954 21.490557246969008 26.436277627849243 27.168217071816798 86-87 25.396883112606407 21.028767679650496 26.435159521627217 27.139189686115884 88-89 25.81831555719014 20.187404280546513 26.846459134998053 27.14782102726529 90-91 26.76274421291373 20.316442583295487 26.11985659030132 26.800956613489458 92-93 27.108567241210142 20.68082064047612 26.01013964830282 26.200472470010915 94-95 26.14680220989623 21.252411756472288 25.67638962457563 26.924396409055856 96-97 25.908936540988165 21.830599874447277 24.664112160433056 27.59635142413151 98-99 25.868327477821435 22.28854124002385 24.584574242196595 27.258557039958127 100-101 25.293216101776313 23.46586371831496 24.555099106219146 26.68582107368958 102-103 25.23258438451665 24.733490985104034 24.625371232873896 25.408553397505422 104-105 24.724930949744586 25.8912045136159 24.892737134287483 24.49112740235203 106-107 23.741683371957887 27.036364709094673 24.638417916304775 24.583534002642658 108-109 23.32297872457198 28.63703345947296 24.651217992870684 23.388769823084374 110-111 23.511707934173227 30.666404142575278 23.14529473588887 22.67659318736262 112-113 23.152298744118845 32.11346974730074 22.563668770092658 22.170562738487757 114-115 22.75070854764713 32.91311020685955 22.74069180046914 21.595489445024178 116-117 22.701908010460716 33.43470696548677 22.31491365518604 21.548471368866473 118-119 22.902415246673716 33.52071290282717 22.47882025017507 21.098051600324048 120-121 23.267993765161744 33.42917476207726 22.307315506964798 20.995515965796205 122-123 23.906048464557205 33.79113038228272 21.843629078734324 20.459192074425754 124-125 24.700304285787965 34.986814282521614 20.566624834533858 19.74625659715656 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8.0 1 7.5 2 6.5 3 8.5 4 9.5 5 8.0 6 8.0 7 9.0 8 9.0 9 12.5 10 14.0 11 15.5 12 20.0 13 17.5 14 26.0 15 38.5 16 48.5 17 71.0 18 94.0 19 127.0 20 173.0 21 246.5 22 362.0 23 504.5 24 680.5 25 887.0 26 1129.0 27 1384.5 28 1660.0 29 1975.5 30 2297.5 31 2564.5 32 2791.5 33 3011.5 34 3133.0 35 3197.0 36 3245.5 37 3300.5 38 3293.5 39 3208.5 40 3067.0 41 2939.5 42 2861.5 43 2796.0 44 2837.0 45 3134.0 46 3725.0 47 4803.5 48 7050.5 49 11922.5 50 21286.5 51 36089.5 52 56184.0 53 76190.0 54 87587.0 55 86448.0 56 75480.0 57 60003.5 58 43459.5 59 29274.0 60 19128.0 61 12628.5 62 8850.5 63 6356.5 64 4887.5 65 4153.0 66 3601.0 67 3033.5 68 2429.5 69 1780.0 70 1239.5 71 957.5 72 704.0 73 508.0 74 395.5 75 289.0 76 222.0 77 179.0 78 150.5 79 122.5 80 91.5 81 74.5 82 62.0 83 53.0 84 43.0 85 30.0 86 23.0 87 21.0 88 19.0 89 15.0 90 9.0 91 5.5 92 5.0 93 3.5 94 3.5 95 2.5 96 1.5 97 1.5 98 1.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14708666862416336 2 0.015504471599375432 3 0.00617434709709641 4 0.01413239446668734 5 0.0028813619786449917 6 0.0 7 2.7441542653761824E-4 8 4.116231398064274E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0013720771326880912 28-29 0.008026651226225333 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 1.3720771326880912E-4 52-53 0.0 54-55 0.0 56-57 8.232462796128548E-4 58-59 0.0 60-61 6.860385663440456E-5 62-63 0.0 64-65 0.0022639272689353503 66-67 2.058115699032137E-4 68-69 0.00617434709709641 70-71 0.018866060574461253 72-73 1.3720771326880912E-4 74-75 0.008918501362472592 76-77 2.7441542653761824E-4 78-79 0.0013720771326880912 80-81 0.001577888702591305 82-83 0.0 84-85 0.019414891427536492 86-87 0.002469738838838564 88-89 0.017837002724945185 90-91 1.3720771326880912E-4 92-93 0.0135149597569777 94-95 0.013720771326880911 96-97 0.006105743240462006 98-99 0.0029499658352793957 100-101 0.001097661706150473 102-103 6.860385663440456E-5 104-105 6.860385663440456E-4 106-107 0.0015092848459569003 108-109 0.0 110-111 0.0 112-113 0.0015092848459569003 114-115 0.005831327813924388 116-117 0.0 118-119 0.07299450345900645 120-121 0.0019209079857633279 122-123 0.005762723957289983 124-125 0.23455658583302919 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 728822.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.28806928100325 #Duplication Level Percentage of deduplicated Percentage of total 1 82.48131282858199 51.37601727854817 2 9.07176071373928 11.301249196761518 3 3.059568132903435 5.717237754967168 4 1.5628052479177492 3.8937648622006495 5 0.9064984586894822 2.823201939898657 6 0.6144444699369441 2.296353582765702 7 0.42521731559443404 1.8540175929259832 8 0.33624015457096185 1.6755000034377048 9 0.24101373799947343 1.3511052369166288 >10 1.254576503124265 13.209813525405977 >50 0.030894640288138882 1.3375693345417412 >100 0.013902411115986741 1.8278509366888667 >500 4.4134638447065406E-4 0.1623320766905517 >1k 0.0013240391534119621 1.1739866782506367 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT 1792 0.24587622217770597 No Hit ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA 1737 0.23832979794792142 No Hit GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT 1534 0.2104766321543532 No Hit GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA 1346 0.18468158205981708 No Hit TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC 1077 0.14777270719050742 No Hit ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC 1055 0.14475413749859362 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 1.3720771326880912E-4 0.0 0.0 1.3720771326880912E-4 0.0 3 1.3720771326880912E-4 0.0 0.0 1.3720771326880912E-4 0.0 4 1.3720771326880912E-4 0.0 0.0 1.3720771326880912E-4 0.0 5 4.1162313980642735E-4 0.0 0.0 1.3720771326880912E-4 0.0 6 6.860385663440456E-4 0.0 0.0 1.3720771326880912E-4 0.0 7 0.0013720771326880912 0.0 0.0 1.3720771326880912E-4 0.0 8 0.0016464925592257094 0.0 0.0 1.3720771326880912E-4 0.0 9 0.0016464925592257094 0.0 0.0 2.7441542653761824E-4 0.0 10-11 0.0019895118423977325 0.0 0.0 3.430192831720228E-4 0.0 12-13 0.0023325311255697548 0.0 0.0 4.802269964408319E-4 0.0 14-15 0.0028813619786449913 0.0 0.0 7.546424229784501E-4 0.0 16-17 0.003430192831720228 0.0 0.0 0.001097661706150473 0.0 18-19 0.00391041982816106 0.0 0.0 0.0019209079857633276 0.0 20-21 0.004116231398064274 0.0 0.0 0.004322042967967487 0.0 22-23 0.004459250681236296 0.0 0.0 0.01728817187186995 0.0 24-25 0.0046650622511395096 0.0 0.0 0.04109371012400833 0.0 26-27 0.005899931670558792 0.0 0.0 0.10173951938882196 0.0 28-29 0.006585970236902838 0.0 0.0 0.3611307013235056 0.0 30-31 0.007752235799687715 0.0 0.0 0.9147638243631504 0.0 32-33 0.009535936072182234 0.0 0.0 1.6995919442607383 0.0 34-35 0.010359182351795088 0.0 0.0 2.8471972580410583 0.0 36-37 0.01173125948448318 0.0 0.0 4.399222306681192 0.0 38-39 0.012417298050827225 0.0 0.0 6.467765791921758 0.0 40-41 0.013240544330440081 0.0 0.0 8.990809827365256 0.0 42-43 0.015435867742741026 0.0 0.0 10.951302238406635 0.0 44-45 0.017425379585138758 0.0 0.0 12.636418768917514 0.0 46-47 0.017837002724945185 0.0 0.0 14.635878170527235 0.0 48-49 0.017837002724945185 0.0 0.0 16.710596002864897 0.0 50-51 0.017837002724945185 0.0 0.0 18.82675056460974 0.0 52-53 0.017837002724945185 0.0 0.0 20.822985584957642 0.0 54-55 0.017837002724945185 0.0 0.0 22.635636684951884 0.0 56-57 0.017837002724945185 0.0 0.0 24.438134414164224 0.0 58-59 0.017837002724945185 0.0 0.0 26.27623754496983 0.0 60-61 0.017905606581579588 0.0 0.0 28.186854952237994 0.0 62-63 0.018111418151482803 0.0 0.0 30.05864257665109 0.0 64-65 0.018180022008117205 0.0 0.0 31.84748813839319 0.0 66-67 0.01838583357802042 0.0 0.0 33.72551871376001 0.0 68-69 0.01838583357802042 0.0 0.0 35.740002359972664 0.0 70-71 0.01838583357802042 0.0 0.0 38.000842455359475 0.0 72-73 0.01838583357802042 0.0 0.0 40.16357903575907 0.0 74-75 0.01838583357802042 0.0 0.0 42.11583898400433 0.0 76-77 0.01838583357802042 0.0 0.0 44.099999725584574 0.0 78-79 0.01838583357802042 0.0 0.0 46.03504010581459 0.0 80-81 0.01838583357802042 0.0 0.0 47.97001188218797 0.0 82-83 0.01838583357802042 0.0 0.0 50.026892711800684 0.0 84-85 0.01838583357802042 0.0 0.0 52.1477809396533 0.0 86-87 0.01838583357802042 0.0 0.0 53.88880961332122 0.0 88-89 0.01838583357802042 0.0 0.0 55.50593972190741 0.0 90-91 0.01838583357802042 0.0 0.0 57.324902376712004 0.0 92-93 0.01838583357802042 0.0 0.0 58.972904220783676 0.0 94-95 0.01838583357802042 0.0 0.0 60.51004223253415 0.0 96-97 0.01838583357802042 0.0 0.0 61.9759694410981 0.0 98-99 0.01838583357802042 0.0 0.0 63.33542346416546 0.0 100-101 0.01838583357802042 0.0 0.0 64.71635049435939 0.0 102-103 0.01838583357802042 0.0 0.0 66.03718054614157 0.0 104-105 0.01838583357802042 0.0 0.0 67.27143253085116 0.0 106-107 0.01838583357802042 0.0 0.0 68.40764960443016 0.0 108-109 0.01838583357802042 0.0 0.0 69.46826522799805 0.0 110-111 0.01838583357802042 0.0 0.0 70.5267541320103 0.0 112-113 0.01838583357802042 0.0 0.0 71.6191333411999 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGATTA 25 5.337672E-4 47.591686 106-107 GTAGGGC 70 5.46225E-5 42.48383 4 GGCATGA 60 0.0013106471 39.651573 8 TAGGGAG 155 5.133188E-9 34.53524 5 CTCTATG 70 0.002792587 33.989395 2 CTTGGTA 125 0.0 33.307323 16-17 GTCCTAG 165 9.309588E-9 32.491238 1 GCAGAGA 825 0.0 31.721258 3 CTTGTGT 75 0.003916847 31.721256 3 TTGTGTT 95 3.292484E-4 31.303875 4 AGTAAGA 295 0.0 30.244799 2 GTCTAAG 100 4.4077885E-4 29.783634 1 GTAACAA 120 3.665797E-5 29.783632 1 TAGAAGG 140 3.0998835E-6 29.740723 2 TGTTAGG 100 4.445173E-4 29.740723 2 TATTAGT 110 1.5825208E-10 29.74072 58-59 TACGTTA 80 0.0053709154 29.73868 4 TATAAGG 185 2.8750947E-8 28.936916 2 GTATACG 250 2.1827873E-11 28.592289 1 GTATTAG 105 5.864663E-4 28.365364 1 >>END_MODULE