FastQCFastQC Report
Thu 26 May 2016
SRR935994_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935994_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences728822
Sequences flagged as poor quality0
Sequence length125
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT88411.2130533930095415No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT48330.6631248782281545RNA PCR Primer, Index 33 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT29250.40133256131126666RNA PCR Primer, Index 33 (95% over 24bp)
ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA18390.25232498470133996No Hit
GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT16580.22749038859968554No Hit
GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT15030.20622319304302011No Hit
GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA13100.17974210438213994No Hit
ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC12010.16478646363583976No Hit
TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC10710.14694946091089456No Hit
TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC8570.11758701027136942No Hit
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTG7510.10304299266487564RNA PCR Primer, Index 15 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTACGT150.004238249359.49478544-45
AAGACTT150.004238249359.494785116-117
GAATCGG300.004237400459.4947859
TAGGGTG1051.1277734E-1051.0165335
CTATACA602.1469647E-549.7599221
TAGAGTT505.2868685E-447.6808784
GTGACGT255.3353823E-447.5958314-15
TATGGGT802.2660934E-644.7008254
GCGTCAG1109.325959E-943.426841
GTAGGGC558.455382E-443.3462524
ACTTAGA300.001309191239.6631924-25
CTTGGTA1200.037.18424216-17
GTCAGAT1304.1367457E-836.67763
CTAGGGT650.001922343436.67764
CTTTACA1156.470382E-736.3463751
TTAGATT501.0630218E-535.69687314-15
GTGTGTT851.692889E-435.059473
TAAAGTT609.297073E-734.7052914-15
AGGGTGG2600.034.326276
TCAAGTG350.00279042933.9970254-55
Produced by FastQC (version 0.11.5)