##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935994_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 728822 Sequences flagged as poor quality 0 Sequence length 125 %GC 51 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.354806797818945 33.0 31.0 34.0 31.0 34.0 2 32.466028742271774 34.0 31.0 34.0 31.0 34.0 3 32.46461275867084 34.0 31.0 34.0 30.0 34.0 4 35.92570202326494 37.0 35.0 37.0 35.0 37.0 5 35.915689976427714 37.0 35.0 37.0 35.0 37.0 6 35.99296398846357 37.0 35.0 37.0 35.0 37.0 7 35.982215136206094 37.0 35.0 37.0 35.0 37.0 8 36.01153916868591 37.0 35.0 37.0 35.0 37.0 9 37.70056611902495 39.0 37.0 39.0 35.0 39.0 10-11 37.6114119771357 39.0 37.0 39.0 35.0 39.0 12-13 37.58593593497452 39.0 37.0 39.0 35.0 39.0 14-15 38.729831838226616 40.0 38.0 41.0 34.0 41.0 16-17 38.66504441413679 40.0 38.0 41.0 34.5 41.0 18-19 38.721802442846126 40.0 38.0 41.0 34.0 41.0 20-21 38.81355598486324 40.0 38.0 41.0 35.0 41.0 22-23 38.66251567598124 40.0 38.0 41.0 34.0 41.0 24-25 38.387795648320164 40.0 38.0 41.0 34.0 41.0 26-27 38.198914824195754 40.0 38.0 41.0 33.0 41.0 28-29 38.12231930430201 40.0 37.0 41.0 33.0 41.0 30-31 37.962075102013934 40.0 37.0 41.0 33.0 41.0 32-33 37.59145785939502 40.0 36.0 41.0 33.0 41.0 34-35 37.46483229101207 40.0 36.0 41.0 33.0 41.0 36-37 37.429722895302284 40.0 36.0 41.0 33.0 41.0 38-39 37.050418346317755 40.0 35.0 41.0 31.5 41.0 40-41 36.789816306313476 39.5 35.0 41.0 31.0 41.0 42-43 36.504643795055586 39.0 35.0 41.0 31.0 41.0 44-45 36.07737142951228 39.0 35.0 41.0 30.5 41.0 46-47 35.54617245363065 39.0 35.0 41.0 28.5 41.0 48-49 34.89973752164452 38.0 35.0 40.5 27.0 41.0 50-51 34.235969825279696 37.0 34.5 40.0 25.5 41.0 52-53 33.7366016667993 36.5 34.0 39.0 23.5 41.0 54-55 33.2730378610964 36.0 34.0 39.0 23.0 41.0 56-57 32.873010831176884 35.0 34.0 38.0 22.0 40.5 58-59 32.525071279407044 35.0 33.5 37.0 22.0 40.0 60-61 32.13466456830337 35.0 33.0 36.5 21.0 39.0 62-63 31.74802558100606 35.0 33.0 36.0 20.0 39.0 64-65 31.45777912851149 35.0 33.0 35.5 19.0 37.0 66-67 31.06397789858155 35.0 33.0 35.0 12.5 37.0 68-69 30.635159888148273 35.0 33.0 35.0 3.5 36.0 70-71 30.213749036115814 35.0 32.0 35.0 2.0 36.0 72-73 29.93278742957814 35.0 32.0 35.0 2.0 35.0 74-75 29.793663610593534 35.0 32.0 35.0 2.0 35.0 76-77 29.657409490931943 35.0 31.0 35.0 2.0 35.0 78-79 29.70463432772337 35.0 32.0 35.0 2.0 35.0 80-81 29.67114947133868 35.0 32.0 35.0 2.0 35.0 82-83 29.558417556001327 35.0 31.5 35.0 2.0 35.0 84-85 29.482660375235653 35.0 31.0 35.0 2.0 35.0 86-87 29.470841302814677 35.0 31.0 35.0 2.0 35.0 88-89 29.401527396264107 35.0 31.0 35.0 2.0 35.0 90-91 29.287365776554495 35.0 31.0 35.0 2.0 35.0 92-93 29.179858730938417 35.0 31.0 35.0 2.0 35.0 94-95 29.135959123078063 35.0 31.0 35.0 2.0 35.0 96-97 29.103189804918074 35.0 31.0 35.0 2.0 35.0 98-99 29.02446962358436 35.0 31.0 35.0 2.0 35.0 100-101 28.922957868999564 35.0 31.0 35.0 2.0 35.0 102-103 28.120309897341187 33.5 29.0 35.0 2.0 35.0 104-105 28.559714168891716 34.5 30.0 35.0 2.0 35.0 106-107 28.409195249320135 35.0 30.0 35.0 2.0 35.0 108-109 28.108152607907005 35.0 29.5 35.0 2.0 35.0 110-111 27.682056798504988 34.5 29.0 35.0 2.0 35.0 112-113 27.10769227602899 34.0 27.0 35.0 2.0 35.0 114-115 26.396869880437198 34.0 24.0 35.0 2.0 35.0 116-117 25.658349363767833 34.0 14.5 35.0 2.0 35.0 118-119 25.028119348757308 34.0 2.0 35.0 2.0 35.0 120-121 24.352295896666128 34.0 2.0 35.0 2.0 35.0 122-123 23.584223582712923 33.0 2.0 35.0 2.0 35.0 124-125 21.298247034255276 29.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15182606306297686 1101 2 0.14897882477229274 1101 3 0.13169245044399958 1101 4 0.16542874700012788 1101 5 0.2510757228659273 1101 6 0.22090426012947972 1101 7 0.15534659230549153 1101 8 0.1801693503541557 1101 9 0.19781198582710857 1101 10-11 0.18453114459828868 1101 12-13 0.2115530986793175 1101 14-15 0.18649885744120098 1101 16-17 0.13399105143786016 1101 18-19 0.2801894420247706 1101 20-21 0.20766724829930183 1101 22-23 0.06046267911315084 1101 24-25 0.2055142463606927 1101 26-27 0.3132716858604496 1101 28-29 0.3094688400396137 1101 30-31 0.42283682280020685 1101 32-33 0.4178194567393092 1101 34-35 0.39175178641588815 1101 36-37 0.4491128902957797 1101 38-39 0.2916405967682607 1101 40-41 0.25351571349173696 1101 42-43 0.29311643058230885 1101 44-45 0.29143863822632454 1101 46-47 0.32087211881943745 1101 48-49 0.23326929818104958 1101 50-51 0.22738793930028578 1101 52-53 0.31477362619686744 1101 54-55 0.36263949476828117 1101 56-57 0.32036622991449804 1101 58-59 0.24130914792615243 1101 60-61 0.24288863937221805 1101 62-63 0.24037411868216196 1101 64-65 0.17306719247853763 1101 66-67 0.1425224774646665 1101 68-69 0.4367720068961489 1101 70-71 0.5190901030303934 1101 72-73 0.5956099401619603 1101 74-75 0.45549261175038325 1101 76-77 0.6124295215217614 1101 78-79 0.49937142557343606 1101 80-81 0.5867656109198514 1101 82-83 0.6422152726874728 1101 84-85 0.6802561323480987 1101 86-87 0.4772329391572434 1101 88-89 0.3610626812423625 1101 90-91 0.30780657154949864 1101 92-93 0.35081998334472075 1101 94-95 0.29428038086687636 1101 96-97 0.5351152084858235 1101 98-99 0.44580288785108735 1101 100-101 0.26799804427333385 1101 102-103 0.2623163604015488 1101 104-105 0.4556439054827024 1101 106-107 0.223144176911088 1101 108-109 0.23501368902129016 1101 110-111 0.2782173330374995 1101 112-113 0.5390344992358571 1101 114-115 0.6934755350915793 1101 116-117 0.5745554798023171 1101 118-119 0.08526917636833886 1101 120-121 0.13867553172966396 1101 122-123 0.06256110363523604 1101 124-125 0.05627033385636793 1102 1 0.00825382846328182 1102 2 -0.012825263972565892 1102 3 0.07945309265792844 1102 4 0.003840058401230806 1102 5 0.19017213532826815 1102 6 0.16590327957657536 1102 7 0.24524239650354218 1102 8 0.14898133817600723 1102 9 0.28400740841325955 1102 10-11 0.31112373933416393 1102 12-13 0.1023162903307906 1102 14-15 0.17350412027760598 1102 16-17 0.16253800063236667 1102 18-19 0.11443902971031861 1102 20-21 0.13981459485884784 1102 22-23 0.2592191451361927 1102 24-25 0.17034454640987917 1102 26-27 0.1851407482911256 1102 28-29 0.1260732978581629 1102 30-31 0.222625602060063 1102 32-33 0.19876072322786342 1102 34-35 0.33564165585768535 1102 36-37 0.40736384111060886 1102 38-39 0.35593432540908765 1102 40-41 0.17051592889188072 1102 42-43 0.3267911892216162 1102 44-45 0.02319955049416933 1102 46-47 -0.1224310263441879 1102 48-49 0.1479981672659889 1102 50-51 -0.15395872955252798 1102 52-53 -0.02842631979234511 1102 54-55 0.025113895907644235 1102 56-57 -0.05015577787205672 1102 58-59 -0.029431153441159097 1102 60-61 0.007121927375074222 1102 62-63 -0.010128213747776726 1102 64-65 0.05483019444928772 1102 66-67 0.02791513264117995 1102 68-69 0.13981557880925521 1102 70-71 0.0659508870226233 1102 72-73 0.08597480228979393 1102 74-75 0.1622349578116129 1102 76-77 0.2611777894795395 1102 78-79 0.2929553946586907 1102 80-81 0.25518280563836626 1102 82-83 0.22326359626350012 1102 84-85 0.11185211883254453 1102 86-87 0.16917697369842344 1102 88-89 0.10944637641579291 1102 90-91 0.3755884965838163 1102 92-93 0.38289049313578616 1102 94-95 0.286326650104197 1102 96-97 0.31832621382582005 1102 98-99 0.40177927742314523 1102 100-101 0.2882327982831505 1102 102-103 0.2312681975719002 1102 104-105 0.5073769121793958 1102 106-107 0.575021212362099 1102 108-109 0.4549099111599233 1102 110-111 0.5880768728527705 1102 112-113 0.5294508002045148 1102 114-115 0.2524308765329586 1102 116-117 0.5510511444725132 1102 118-119 0.39318689994243883 1102 120-121 0.5370066628376264 1102 122-123 0.3112952259117918 1102 124-125 -0.04286034973367947 1103 1 -0.005056821487166019 1103 2 0.05326205683381602 1103 3 0.09569623399992366 1103 4 0.0995486075843175 1103 5 0.18478329923103587 1103 6 0.1306551417596964 1103 7 0.10091170956487616 1103 8 0.10238129117440309 1103 9 0.21870276649395493 1103 10-11 0.20151242216529397 1103 12-13 0.04126069560723522 1103 14-15 0.18174319495906843 1103 16-17 0.35686460047501356 1103 18-19 0.39565626908130724 1103 20-21 0.28741751304539775 1103 22-23 0.2812491035499889 1103 24-25 0.4728024237765567 1103 26-27 0.3996632222103429 1103 28-29 0.5156762364801963 1103 30-31 0.39598539934010546 1103 32-33 0.42217722896512555 1103 34-35 0.5293056352587158 1103 36-37 0.6256183445795216 1103 38-39 0.5919094554298283 1103 40-41 0.38931452701795166 1103 42-43 0.5187186637028134 1103 44-45 0.6072946163474953 1103 46-47 0.3916055940361076 1103 48-49 0.4829387511076533 1103 50-51 0.37323425290361456 1103 52-53 0.2232958802914382 1103 54-55 0.17271732498120684 1103 56-57 0.2436032265113539 1103 58-59 0.35519932199029114 1103 60-61 0.16342933830193118 1103 62-63 0.128141820686011 1103 64-65 0.2627755091045927 1103 66-67 0.25472341718480607 1103 68-69 0.13936497647348034 1103 70-71 0.3948624933535356 1103 72-73 0.4410203223216733 1103 74-75 0.3910811706062667 1103 76-77 0.4044167108948997 1103 78-79 0.40988210280938375 1103 80-81 0.3673306767722302 1103 82-83 0.4491762958925989 1103 84-85 0.4445591047014119 1103 86-87 0.4742143195382731 1103 88-89 0.5885608048865016 1103 90-91 0.2786798738329992 1103 92-93 0.4232766215319046 1103 94-95 0.6158584732555532 1103 96-97 0.5585110527431496 1103 98-99 0.6604122459831601 1103 100-101 0.5180890346807843 1103 102-103 0.5774110007067037 1103 104-105 0.37504593914400886 1103 106-107 0.34039431313966517 1103 108-109 0.4607948185361117 1103 110-111 0.4698729471970431 1103 112-113 0.17955093405691258 1103 114-115 0.35799750130689034 1103 116-117 0.29692215572224256 1103 118-119 0.38939407483949395 1103 120-121 0.6282291132560403 1103 122-123 0.22487102621628097 1103 124-125 0.251285396110255 1104 1 0.03256694088907608 1104 2 0.12058878950708163 1104 3 0.1867853429108166 1104 4 -0.006391986475087208 1104 5 0.05607042794390793 1104 6 0.05144722096761711 1104 7 -0.06344472607868568 1104 8 -0.17583653060777493 1104 9 -0.11001010479316875 1104 10-11 0.12576984790786838 1104 12-13 -0.006759106372960844 1104 14-15 6.002664223814236E-5 1104 16-17 0.14349826384135156 1104 18-19 0.13427013046744918 1104 20-21 0.04187295858996265 1104 22-23 0.2094669253321726 1104 24-25 0.19359450298448166 1104 26-27 0.11996025191331938 1104 28-29 0.17904257311385408 1104 30-31 0.17370817161733498 1104 32-33 0.190494060648291 1104 34-35 0.13128583327851828 1104 36-37 0.061261908935961173 1104 38-39 0.169632227707055 1104 40-41 0.16951254681992367 1104 42-43 -0.10603381154471236 1104 44-45 -0.10310142325645444 1104 46-47 -0.12869143566685892 1104 48-49 -0.19824936770422852 1104 50-51 -0.3534984203637208 1104 52-53 -0.336110060302623 1104 54-55 -0.32579752650394056 1104 56-57 -0.3618423180431023 1104 58-59 -0.17945414335624577 1104 60-61 -0.16775878050995274 1104 62-63 -0.3094819416902297 1104 64-65 -0.27880864931124805 1104 66-67 -0.03983103826073986 1104 68-69 0.0472857685526904 1104 70-71 -0.0046424571415144555 1104 72-73 -0.10155393510406796 1104 74-75 -0.05842377988878056 1104 76-77 0.058377106934507594 1104 78-79 0.01037715231433367 1104 80-81 -0.04999605590103684 1104 82-83 0.04323570183318992 1104 84-85 0.053469995790518254 1104 86-87 0.05540243835015346 1104 88-89 0.11875882468848431 1104 90-91 0.017788784724089624 1104 92-93 0.03317761163091859 1104 94-95 0.2658584732555518 1104 96-97 0.1822734289807748 1104 98-99 0.02278848360692365 1104 100-101 -0.02349512373506002 1104 102-103 0.02840109971660354 1104 104-105 -0.0704986153114362 1104 106-107 0.05227550125847813 1104 108-109 -0.08227448839458162 1104 110-111 0.08967492739506255 1104 112-113 -0.0788649075272474 1104 114-115 -0.2340817066138996 1104 116-117 0.018704333940068096 1104 118-119 0.05820595602761003 1104 120-121 -0.15048375803108982 1104 122-123 -0.25285174606094785 1104 124-125 -0.25564529695905236 1105 1 0.10551049116827471 1105 2 0.10434835029728617 1105 3 0.10846428805651698 1105 4 0.1750166757181475 1105 5 0.11783195954730985 1105 6 0.13856185660179676 1105 7 0.14429306191812685 1105 8 0.2041268685757487 1105 9 0.05944698309571095 1105 10-11 0.12852429396858867 1105 12-13 0.07375799747012479 1105 14-15 0.11423111199722058 1105 16-17 0.10632966546492639 1105 18-19 0.11552628925868191 1105 20-21 0.05428204310030793 1105 22-23 0.12677254214977296 1105 24-25 0.19140549261425122 1105 26-27 0.15219477868352982 1105 28-29 0.1037270083820303 1105 30-31 0.08264252476828915 1105 32-33 0.06984979243000566 1105 34-35 0.1577698086902828 1105 36-37 0.22196530155010663 1105 38-39 0.16915360164539806 1105 40-41 -0.0029242229233901185 1105 42-43 -0.11272284537700017 1105 44-45 -0.12151210318700834 1105 46-47 0.17038415909640747 1105 48-49 0.23299271384989595 1105 50-51 0.2583053429510045 1105 52-53 0.2099412746887026 1105 54-55 0.2737402708776884 1105 56-57 0.29946639576666456 1105 58-59 0.30024178832223214 1105 60-61 0.17209387774165208 1105 62-63 0.18811788938292295 1105 64-65 0.2930368764735576 1105 66-67 0.30144576714491933 1105 68-69 0.31259007149483153 1105 70-71 0.2807876024521221 1105 72-73 0.4461702448968694 1105 74-75 0.49051104250341027 1105 76-77 0.35407416479194964 1105 78-79 0.3645252448487071 1105 80-81 0.2870904252589277 1105 82-83 0.2133520736599479 1105 84-85 0.16728915021781177 1105 86-87 0.25391963604148415 1105 88-89 0.332296434373621 1105 90-91 0.3281261691734336 1105 92-93 0.34087082171021876 1105 94-95 0.3184416462647981 1105 96-97 0.26555670991550073 1105 98-99 0.22501034086374005 1105 100-101 0.1790387789443102 1105 102-103 0.23516286594061953 1105 104-105 0.146938389052508 1105 106-107 0.2730863501158787 1105 108-109 0.10931201622744524 1105 110-111 -0.004506481267128493 1105 112-113 0.08526394611640953 1105 114-115 0.04857044485716955 1105 116-117 0.06296551361253577 1105 118-119 0.058452307676997606 1105 120-121 -0.057466067636454454 1105 122-123 -0.394445289301526 1105 124-125 -0.20412495535143194 1106 1 0.16572090174820886 1106 2 0.16121063466634666 1106 3 0.041933579904771534 1106 4 0.14649819496588634 1106 5 0.22587287123781863 1106 6 0.30145138883062117 1106 7 0.203297579583122 1106 8 0.17869671504607254 1106 9 0.2812975853771533 1106 10-11 0.2735289269027632 1106 12-13 0.29416152825364605 1106 14-15 0.26901500445993776 1106 16-17 0.40997660484663356 1106 18-19 0.17683929383841246 1106 20-21 0.27511073980493705 1106 22-23 0.37166811271002587 1106 24-25 0.27732270274552207 1106 26-27 0.3055010089824748 1106 28-29 0.4106085781708657 1106 30-31 0.40810415564978086 1106 32-33 0.6163852886859758 1106 34-35 0.3525724988955048 1106 36-37 0.5072339008966225 1106 38-39 0.32217786580687857 1106 40-41 0.42279543357445704 1106 42-43 0.42172091434883185 1106 44-45 0.29114090556014816 1106 46-47 0.4464662484832189 1106 48-49 0.573378599582675 1106 50-51 0.6797356560841479 1106 52-53 0.6647207337215946 1106 54-55 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14-15 -0.6719426850405981 2216 16-17 -0.7801201588631415 2216 18-19 -0.7300490613841149 2216 20-21 -0.6732609935203868 2216 22-23 -1.029998940104754 2216 24-25 -1.0482863503586657 2216 26-27 -1.0703509220612304 2216 28-29 -1.3497678015837948 2216 30-31 -1.2782096744360842 2216 32-33 -1.3030795021179529 2216 34-35 -1.5621165754323414 2216 36-37 -1.5196194716788511 2216 38-39 -1.7236965303589429 2216 40-41 -1.7698562336760446 2216 42-43 -1.7009966932935825 2216 44-45 -1.457815901466752 2216 46-47 -1.6342369944492816 2216 48-49 -1.8380324330775508 2216 50-51 -1.651660083529194 2216 52-53 -1.4211188063166986 2216 54-55 -1.7708779982028489 2216 56-57 -1.4622319132156392 2216 58-59 -1.309609060792198 2216 60-61 -1.2272082419396142 2216 62-63 -1.427572799803933 2216 64-65 -1.4222784316233792 2216 66-67 -1.6410776591189347 2216 68-69 -1.505348497409834 2216 70-71 -1.5082722622183837 2216 72-73 -1.1848942429302873 2216 74-75 -1.177737406095023 2216 76-77 -1.5129359668918951 2216 78-79 -1.4022585364447373 2216 80-81 -1.388413655057402 2216 82-83 -1.6360487988907586 2216 84-85 -1.829939359515258 2216 86-87 -2.0546592440649185 2216 88-89 -1.9281122783136162 2216 90-91 -1.7606985314631522 2216 92-93 -2.3030555763539837 2216 94-95 -2.125637388080367 2216 96-97 -2.0829571896009504 2216 98-99 -2.2455120277628957 2216 100-101 -2.076515218643955 2216 102-103 -2.1208686708783766 2216 104-105 -2.112481993699994 2216 106-107 -2.291487209754802 2216 108-109 -2.154042857116494 2216 110-111 -2.1757335407552176 2216 112-113 -1.779795165388201 2216 114-115 -1.7426896257321047 2216 116-117 -1.5827640757918608 2216 118-119 -1.3871314559891594 2216 120-121 -1.739567560747794 2216 122-123 -1.505184713930852 2216 124-125 -1.7753271763896024 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 13.0 4 44.0 5 150.0 6 399.0 7 962.0 8 1501.0 9 2089.0 10 3037.0 11 5445.0 12 7570.0 13 8239.0 14 11041.0 15 13854.0 16 6677.0 17 3419.0 18 3406.0 19 4229.0 20 6103.0 21 8168.0 22 7881.0 23 3578.0 24 4408.0 25 5761.0 26 7309.0 27 9313.0 28 11356.0 29 13986.0 30 18530.0 31 25232.0 32 34044.0 33 45544.0 34 63107.0 35 95607.0 36 207262.0 37 85153.0 38 4400.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.52008022467307 19.587274574304736 11.516969387881346 16.375675813140845 2 18.59349573416537 21.09687794142264 36.53867790684592 23.770948417566075 3 30.525560424904846 22.108964877569555 27.083293314416963 20.282181383108632 4 16.122500841351933 21.8972657779243 27.65527235763982 34.32496102308395 5 16.004754349461155 33.31160204008806 27.526091276921644 23.157552333529143 6 32.424307287850475 34.828115180803564 9.452910392671809 23.294667138674154 7 27.687967706792605 39.27008240695259 11.929113007016802 21.112836879238003 8 36.46116610091353 26.863623765473598 12.791600692624536 23.883609440988334 9 34.318393242794535 14.629498011860234 17.26278844491522 33.789320300430006 10-11 28.915359305838738 25.61743470970964 17.97105466080881 27.49615132364281 12-13 32.97636459931231 25.922173040879663 19.471969836256314 21.629492523551704 14-15 35.780272823817064 19.361243211648386 23.03244139172528 21.826042572809275 16-17 30.611658264981024 21.38018610854228 25.175900288410613 22.832255338066084 18-19 29.863327396812938 21.855473627305432 24.950811034793134 23.330387941088496 20-21 34.87854373221445 19.694795162604862 22.993268589587032 22.433392515593656 22-23 30.625036017024733 23.047328428614943 22.331378580778296 23.996256973582028 24-25 26.583514218835326 24.766403868159852 23.004862641358244 25.645219271646575 26-27 24.78938616013238 23.45538416787638 25.626696230355282 26.128533441635955 28-29 26.094505928745292 20.63384475221659 29.088035212987535 24.183614106050584 30-31 28.63785670575257 21.884081435521978 25.742362332640887 23.73569952608456 32-33 24.284530379159794 20.93583892912124 28.50497103545173 26.274659656267236 34-35 22.695047624797276 21.117707753059044 28.479381796927093 27.707862825216584 36-37 24.447327330953236 23.928476363227237 24.98627922867312 26.63791707714641 38-39 26.246943698186936 18.62615288781074 27.388511872583425 27.738391541418896 40-41 23.67169212784466 19.362340873354537 28.87474582271117 28.091221176089636 42-43 25.056803993293286 19.15817579601055 29.936459107985215 25.84856110271095 44-45 25.300965119055135 18.43358186223797 30.265757619830357 25.999695398876543 46-47 23.25259116766508 18.723364552661693 29.661220435167984 28.362823844505243 48-49 22.533073919283446 19.903419490630085 29.642217166880254 27.921289423206215 50-51 22.649426060135397 20.42714133217713 28.410572128722787 28.512860478964686 52-53 22.050102768577236 20.88198490166323 27.423156820183802 29.644755509575727 54-55 20.083504614295396 21.42553325777762 25.77426312597589 32.71669900195109 56-57 20.672537327358395 21.01171479455889 25.914077785796806 32.401670092285904 58-59 21.027630889298074 20.397778881537604 26.794676889556023 31.7799133396083 60-61 21.91358109387477 22.404853311233744 23.941854115270942 31.739711479620535 62-63 19.88084882179737 23.120254328217314 24.443348307268444 32.55554854271688 64-65 18.959704838767216 23.308846330105293 23.94288317312046 33.78856565800703 66-67 20.436265645109504 24.904160412281737 22.36897349421395 32.290600448394805 68-69 20.79732774257638 24.16159226807094 22.786359357977666 32.25472063137501 70-71 20.195466108322748 24.44911103122573 23.160936415201515 32.19448644525001 72-73 21.050407369700693 24.02122877739695 23.13898318107851 31.789380671823846 74-75 21.433216889720672 23.499222032265767 23.434116972319714 31.633444105693847 76-77 21.0763396275085 23.530573994747687 23.80615568684809 31.58693069089572 78-79 21.021936769197417 23.81404513036105 24.17620488953407 30.98781321090747 80-81 20.666911811114375 24.873563092222792 23.64239828106177 30.817126815601064 82-83 19.690061496497087 25.018317229721386 24.005312682657767 31.286308591123756 84-85 19.81828210454679 23.97649906287132 24.094977923278936 32.11024090930296 86-87 20.307702017776634 23.447494724363423 24.287548948851708 31.95725430900824 88-89 20.438117949238634 23.393023262195708 24.557916747847898 31.61094204071776 90-91 20.619643753893268 22.911835811761993 24.340922749313275 32.12759768503146 92-93 20.027112244141918 22.90524984152509 24.520664853695415 32.546973060637576 94-95 19.481711583898402 22.89413601675032 24.693820987840652 32.93033141151063 96-97 19.783774364659685 23.312962561503358 24.247141277294045 32.65612179654291 98-99 20.42851340930982 23.410242830210944 24.124546185488363 32.036697574990875 100-101 20.478525620796297 24.458646967297916 23.784476868151618 31.27835054375417 102-103 20.222976254833142 25.586219954940987 23.381909437420934 30.808894352804938 104-105 20.15361775577576 27.156973856442313 23.28805936154507 29.40134902623686 106-107 19.649996844222596 29.320053456125088 22.564014258625562 28.46593544102675 108-109 19.20976589619962 30.4690308470381 22.213928778220197 28.107274478542088 110-111 19.03110815958025 31.782064510463602 21.814336207417 27.372491122539145 112-113 18.938695425482287 33.03456271781784 21.338190499711864 26.68855135698801 114-115 18.441135590592452 33.561144810988736 21.31516878676324 26.68255081165557 116-117 18.15585329759331 33.82227732180358 21.46432092472161 26.557548455881495 118-119 18.221135210600877 34.27468844653767 21.795055621683364 25.709120721178092 120-121 18.572967262149774 34.50399275541286 21.595867292335555 25.327172690101808 122-123 18.330630267205635 34.35075644688132 21.60909273026144 25.709520555651604 124-125 17.299296620346542 36.07342597358038 21.51291816778178 25.1143592382913 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.5 3 1.0 4 1.0 5 1.5 6 2.0 7 4.0 8 7.5 9 13.5 10 21.0 11 24.5 12 21.5 13 18.0 14 16.5 15 15.5 16 20.5 17 32.5 18 47.0 19 64.0 20 87.5 21 142.0 22 218.0 23 307.0 24 475.5 25 717.0 26 980.0 27 1304.5 28 1750.5 29 2220.5 30 2707.0 31 3171.0 32 3538.0 33 3836.5 34 4056.5 35 4094.0 36 4000.5 37 3899.0 38 3771.5 39 3662.0 40 3518.0 41 3453.5 42 3647.0 43 3878.5 44 4175.0 45 4655.0 46 5191.0 47 6268.0 48 8508.0 49 13425.0 50 23315.5 51 39681.5 52 60960.5 53 79334.5 54 86648.5 55 82788.0 56 71802.5 57 56056.0 58 39643.0 59 26520.0 60 17004.5 61 10990.5 62 7582.5 63 5290.0 64 4008.0 65 3477.5 66 3040.0 67 2535.5 68 1960.0 69 1354.0 70 858.0 71 617.0 72 426.0 73 307.0 74 234.5 75 134.5 76 88.0 77 60.0 78 42.0 79 38.0 80 23.5 81 11.0 82 7.5 83 4.5 84 3.0 85 2.0 86 1.0 87 1.0 88 0.5 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18728852861192447 2 0.001097661706150473 3 0.0 4 0.11347077887330514 5 0.031008943198750864 6 0.0027441542653761824 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 0.0 108-109 0.0 110-111 0.0013720771326880912 112-113 2.7441542653761824E-4 114-115 4.116231398064274E-4 116-117 0.005556912387386769 118-119 0.001989511842397732 120-121 2.7441542653761824E-4 122-123 0.0027441542653761824 124-125 0.0270299195139554 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 728822.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.372908398208565 #Duplication Level Percentage of deduplicated Percentage of total 1 79.97423591721383 45.083802755778166 2 10.182466221260055 11.480304711178922 3 3.525793119737017 5.9627763770990665 4 1.7501835992110089 3.946517588734767 5 1.0444437769205572 2.943916668171078 6 0.7216641105473688 2.4409382878896895 7 0.516726567352414 2.0390565613794864 8 0.3721358819682829 1.6782705588707654 9 0.3007144398597207 1.5256932815007571 >10 1.5421087206572304 14.80744011770628 >50 0.04683978076944465 1.7630780969569346 >100 0.01878457598659003 2.249768673491987 >500 0.0017076887259572125 0.6154236086309306 >1k 0.001951644258236814 2.247155701682549 >5k 2.4395553227960176E-4 1.215857010928728 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT 8841 1.2130533930095415 No Hit ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT 4833 0.6631248782281545 RNA PCR Primer, Index 33 (95% over 22bp) ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT 2925 0.40133256131126666 RNA PCR Primer, Index 33 (95% over 24bp) ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA 1839 0.25232498470133996 No Hit GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT 1658 0.22749038859968554 No Hit GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT 1503 0.20622319304302011 No Hit GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA 1310 0.17974210438213994 No Hit ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC 1201 0.16478646363583976 No Hit TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC 1071 0.14694946091089456 No Hit TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC 857 0.11758701027136942 No Hit CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTG 751 0.10304299266487564 RNA PCR Primer, Index 15 (95% over 24bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 1.3720771326880912E-4 0.0 0.0 0.0 0.0 3 1.3720771326880912E-4 0.0 0.0 0.0 0.0 4 1.3720771326880912E-4 0.0 0.0 0.0 0.0 5 1.3720771326880912E-4 0.0 0.0 0.0 0.0 6 1.3720771326880912E-4 0.0 0.0 0.0 0.0 7 2.7441542653761824E-4 0.0 0.0 0.0 0.0 8 5.488308530752365E-4 0.0 0.0 0.0 0.0 9 5.488308530752365E-4 0.0 0.0 0.0 0.0 10-11 6.860385663440456E-4 0.0 0.0 0.0 0.0 12-13 9.604539928816638E-4 0.0 0.0 6.860385663440456E-5 0.0 14-15 0.0013720771326880912 0.0 0.0 3.430192831720228E-4 0.0 16-17 0.0013720771326880912 0.0 0.0 8.232462796128547E-4 0.0 18-19 0.0015092848459569003 0.0 0.0 0.0017150964158601139 0.0 20-21 0.0017837002724945185 0.0 0.0 0.0038418159715266553 0.0 22-23 0.0019895118423977325 0.0 0.0 0.016876548732063523 0.0 24-25 0.002058115699032137 0.0 0.0 0.04068208698420191 0.0 26-27 0.0026069465521073735 0.0 0.0 0.10132789624901553 0.0 28-29 0.003292985118451419 0.0 0.0 0.36023885118725835 0.0 30-31 0.0038418159715266553 0.0 0.0 0.9135289549437311 0.0 32-33 0.003979023684795464 0.0 0.0 1.6984256786979537 0.0 34-35 0.0046650622511395096 0.0 0.0 2.8472658618976925 0.0 36-37 0.005488308530752365 0.0 0.0 4.399565325964365 0.0 38-39 0.005488308530752365 0.0 0.0 6.470578550043769 0.0 40-41 0.005556912387386769 0.0 0.0 8.99931670558792 0.0 42-43 0.006174347097096411 0.0 0.0 10.963650932600828 0.0 44-45 0.0067231779501716465 0.0 0.0 12.65007093638776 0.0 46-47 0.0067231779501716465 0.0 0.0 14.654675627245062 0.0 48-49 0.0067231779501716465 0.0 0.0 16.73714569538241 0.0 50-51 0.0067231779501716465 0.0 0.0 18.859886227364157 0.0 52-53 0.0067231779501716465 0.0 0.0 20.86325604880204 0.0 54-55 0.0067231779501716465 0.0 0.0 22.686472142717975 0.0 56-57 0.0067231779501716465 0.0 0.0 24.50159298155105 0.0 58-59 0.0067231779501716465 0.0 0.0 26.347242536586435 0.0 60-61 0.0067231779501716465 0.0 0.0 28.27274698074427 0.0 62-63 0.0067231779501716465 0.0 0.0 30.170947089961608 0.0 64-65 0.0067231779501716465 0.0 0.0 31.977218031288846 0.0 66-67 0.0067231779501716465 0.0 0.0 33.87301700552398 0.0 68-69 0.0067231779501716465 0.0 0.0 35.91850959493539 0.0 70-71 0.0067231779501716465 0.0 0.0 38.20919236795815 0.0 72-73 0.0067231779501716465 0.0 0.0 40.389011308659725 0.0 74-75 0.0067231779501716465 0.0 0.0 42.359657090483 0.0 76-77 0.0067231779501716465 0.0 0.0 44.360900192365214 0.0 78-79 0.0067231779501716465 0.0 0.0 46.31707056043862 0.0 80-81 0.0067231779501716465 0.0 0.0 48.27763157533664 0.0 82-83 0.0067231779501716465 0.0 0.0 50.35948420876428 0.0 84-85 0.0067231779501716465 0.0 0.0 52.5045209941522 0.0 86-87 0.0067231779501716465 0.0 0.0 54.26448433225122 0.0 88-89 0.0067231779501716465 0.0 0.0 55.90377348653032 0.0 90-91 0.0067231779501716465 0.0 0.0 57.749423041565706 0.0 92-93 0.0067231779501716465 0.0 0.0 59.421093216176246 0.0 94-95 0.0067231779501716465 0.0 0.0 60.97771472321088 0.0 96-97 0.0067231779501716465 0.0 0.0 62.46326263477228 0.0 98-99 0.0067231779501716465 0.0 0.0 63.840142037424776 0.0 100-101 0.0067231779501716465 0.0 0.0 65.24432577501776 0.0 102-103 0.0067231779501716465 0.0 0.0 66.5815521485356 0.0 104-105 0.0067231779501716465 0.0 0.0 67.8298679238552 0.0 106-107 0.0067231779501716465 0.0 0.0 68.98474524643876 0.0 108-109 0.0067231779501716465 0.0 0.0 70.06367809972805 0.0 110-111 0.0067231779501716465 0.0 0.0 71.14254234916069 0.0 112-113 0.0067231779501716465 0.0 0.0 72.2550224883442 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTACGT 15 0.0042382493 59.494785 44-45 AAGACTT 15 0.0042382493 59.494785 116-117 GAATCGG 30 0.0042374004 59.494785 9 TAGGGTG 105 1.1277734E-10 51.016533 5 CTATACA 60 2.1469647E-5 49.759922 1 TAGAGTT 50 5.2868685E-4 47.680878 4 GTGACGT 25 5.3353823E-4 47.59583 14-15 TATGGGT 80 2.2660934E-6 44.700825 4 GCGTCAG 110 9.325959E-9 43.42684 1 GTAGGGC 55 8.455382E-4 43.346252 4 ACTTAGA 30 0.0013091912 39.66319 24-25 CTTGGTA 120 0.0 37.184242 16-17 GTCAGAT 130 4.1367457E-8 36.6776 3 CTAGGGT 65 0.0019223434 36.6776 4 CTTTACA 115 6.470382E-7 36.346375 1 TTAGATT 50 1.0630218E-5 35.696873 14-15 GTGTGTT 85 1.692889E-4 35.05947 3 TAAAGTT 60 9.297073E-7 34.70529 14-15 AGGGTGG 260 0.0 34.32627 6 TCAAGTG 35 0.002790429 33.99702 54-55 >>END_MODULE