FastQCFastQC Report
Thu 26 May 2016
SRR935993_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935993_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences866112
Sequences flagged as poor quality0
Sequence length125
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT10590.1222705608512525No Hit
ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA9460.10922374935343235No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACTTA250.002058446471.3845146
CTAGTCG150.00424055459.487136-37
GCTAGTC150.00424055459.487134-35
TATAAGG1600.048.350012
TCGGAGA558.524234E-443.2758374
TAACAAC1800.043.0200271
TCGATTG300.001310091739.65806658-59
GATTGCG300.001310091739.65806660-61
GTATAGG5700.037.620751
TAAGGGT801.1967205E-437.190174
TAGCTCA401.1992513E-437.17943614-15
GGCATAT650.001940701236.6074458
GTAACAA1051.4720157E-534.037821
ATTCCTA350.00279234233.99262636-37
GTATAAG2153.6379788E-1233.2462461
TATATGG2350.032.919162
GTCCAAG2750.032.490651
GGTAAGG1301.7383645E-632.0315138
CACCGAT750.00391411231.7264547
TAAGATG953.2842692E-431.3180374
Produced by FastQC (version 0.11.5)