##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935993_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 866112 Sequences flagged as poor quality 0 Sequence length 125 %GC 52 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.492724959358604 31.0 31.0 34.0 28.0 34.0 2 30.460387340205425 33.0 31.0 34.0 27.0 34.0 3 30.538081679967487 33.0 31.0 34.0 27.0 34.0 4 33.86123388199217 37.0 35.0 37.0 30.0 37.0 5 33.95710023645903 37.0 35.0 37.0 30.0 37.0 6 34.12876394738787 37.0 35.0 37.0 31.0 37.0 7 34.08259555346191 37.0 35.0 37.0 30.0 37.0 8 34.05384638476317 37.0 35.0 37.0 30.0 37.0 9 35.53089554237789 39.0 37.0 39.0 30.0 39.0 10-11 35.459278938520654 39.0 37.0 39.0 30.0 39.0 12-13 35.39515732376413 39.0 37.0 39.0 30.0 39.0 14-15 36.47149791251016 40.0 37.0 41.0 29.0 41.0 16-17 36.39890914800857 40.0 37.0 41.0 29.5 41.0 18-19 36.44057754655287 40.0 37.0 41.0 29.5 41.0 20-21 36.50356247228996 40.0 37.0 41.0 30.0 41.0 22-23 36.30472502401537 40.0 37.0 41.0 28.5 41.0 24-25 36.096617989359345 40.0 36.0 41.0 28.0 41.0 26-27 35.90012261693638 40.0 36.0 41.0 27.0 41.0 28-29 35.77591350772187 40.0 36.0 41.0 27.0 41.0 30-31 35.6791044345304 40.0 36.0 41.0 27.0 41.0 32-33 35.36714247118156 40.0 35.0 41.0 25.0 41.0 34-35 35.30669936451636 40.0 35.0 41.0 25.0 41.0 36-37 35.29917897454371 40.0 35.0 41.0 24.5 41.0 38-39 35.20668112207197 40.0 35.0 41.0 23.5 41.0 40-41 34.977585462388234 40.0 35.0 41.0 23.0 41.0 42-43 34.740192376967414 39.0 35.0 41.0 22.0 41.0 44-45 34.42302554404049 39.0 35.0 41.0 21.0 41.0 46-47 34.09862350365772 38.0 34.0 41.0 20.0 41.0 48-49 33.76037625618858 38.0 34.0 40.0 18.5 41.0 50-51 33.42006749704426 37.0 34.0 40.0 16.0 41.0 52-53 33.04354979494569 36.5 34.0 39.5 13.5 41.0 54-55 32.60234299951969 36.0 33.0 39.0 9.5 41.0 56-57 32.16951329620188 35.0 33.0 38.5 7.5 41.0 58-59 31.758860285967636 35.0 33.0 37.0 6.5 40.0 60-61 31.382277927104116 35.0 32.0 37.0 2.0 39.5 62-63 30.955553669733245 35.0 32.0 36.0 2.0 39.0 64-65 30.70717008885687 35.0 32.0 35.5 2.0 38.0 66-67 30.575550275253086 35.0 32.0 35.0 2.0 37.0 68-69 30.43778922356462 35.0 32.0 35.0 2.0 36.5 70-71 30.210940963755263 35.0 32.0 35.0 2.0 36.0 72-73 30.035095922929138 35.0 32.0 35.0 2.0 35.5 74-75 29.84349541398803 35.0 31.5 35.0 2.0 35.0 76-77 29.650354111246582 35.0 31.0 35.0 2.0 35.0 78-79 29.49845343327422 35.0 31.0 35.0 2.0 35.0 80-81 29.330084908187395 35.0 31.0 35.0 2.0 35.0 82-83 29.181543495529446 35.0 31.0 35.0 2.0 35.0 84-85 29.05335799527082 35.0 31.0 35.0 2.0 35.0 86-87 28.929260303517328 35.0 30.0 35.0 2.0 35.0 88-89 28.730139404603563 34.0 30.0 35.0 2.0 35.0 90-91 28.481216055198402 34.0 29.0 35.0 2.0 35.0 92-93 28.15971029150964 34.0 29.0 35.0 2.0 35.0 94-95 27.933185315525012 34.0 28.5 35.0 2.0 35.0 96-97 27.742427076405825 34.0 27.0 35.0 2.0 35.0 98-99 27.45862602065322 34.0 27.0 35.0 2.0 35.0 100-101 27.225283797014704 34.0 25.5 35.0 2.0 35.0 102-103 26.439347336141285 32.5 24.5 34.5 2.0 35.0 104-105 26.947238925219832 33.5 25.0 35.0 2.0 35.0 106-107 26.96837014150595 34.0 25.0 35.0 2.0 35.0 108-109 26.792834529483486 34.0 25.0 35.0 2.0 35.0 110-111 26.486579680226114 34.0 24.5 35.0 2.0 35.0 112-113 26.084089009273626 33.0 23.5 35.0 2.0 35.0 114-115 25.56258486200399 33.0 19.5 35.0 2.0 35.0 116-117 25.0064801088081 33.0 11.5 35.0 2.0 35.0 118-119 24.250934059336437 33.0 2.0 35.0 2.0 35.0 120-121 23.428191735018103 32.5 2.0 35.0 2.0 35.0 122-123 22.533587457511267 32.0 2.0 35.0 2.0 35.0 124-125 19.720410870649523 26.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.35146102087282216 1101 2 0.3588832704574365 1101 3 0.21520068826301397 1101 4 0.2872545049256914 1101 5 0.3512896537165844 1101 6 0.28688568046079865 1101 7 0.22867342310637895 1101 8 0.3050623215697925 1101 9 0.27981936013144093 1101 10-11 0.40204621278036967 1101 12-13 0.14076968431865566 1101 14-15 0.4590402269064171 1101 16-17 0.3641242606992847 1101 18-19 0.4078606309730972 1101 20-21 0.28378813885017706 1101 22-23 0.45766517558934794 1101 24-25 0.39878186264004967 1101 26-27 0.5264574863698712 1101 28-29 0.39934732229185954 1101 30-31 0.17929591353901486 1101 32-33 0.19276100980840027 1101 34-35 -0.12381416132009804 1101 36-37 -0.011329865040863751 1101 38-39 0.23932615129304224 1101 40-41 0.06920665847704299 1101 42-43 0.14267378319223667 1101 44-45 0.23990398802433077 1101 46-47 0.07095542592136184 1101 48-49 0.050498053413981836 1101 50-51 0.17426588395507991 1101 52-53 0.24387699966445808 1101 54-55 0.22979671701256876 1101 56-57 0.09076866142189743 1101 58-59 0.1800173518785364 1101 60-61 0.27382696609091894 1101 62-63 0.12748920967838018 1101 64-65 0.09905237212405993 1101 66-67 0.16779220817270257 1101 68-69 0.3203266379496412 1101 70-71 0.23574572827908113 1101 72-73 0.3648685603027957 1101 74-75 0.31694282519682915 1101 76-77 0.32369460505540815 1101 78-79 0.3927875685115083 1101 80-81 0.3161733558468036 1101 82-83 0.3416140482513761 1101 84-85 0.2950481055595269 1101 86-87 0.27569886852717573 1101 88-89 0.26429052524521524 1101 90-91 0.18463270447655944 1101 92-93 -0.023285225345087923 1101 94-95 0.03948108703998798 1101 96-97 0.10501625518759639 1101 98-99 0.0879658092957527 1101 100-101 0.13173301033578255 1101 102-103 0.29806379447442666 1101 104-105 0.271459220593659 1101 106-107 0.42053754174546043 1101 108-109 0.2683816126547711 1101 110-111 0.24955025508462825 1101 112-113 0.2179564404070753 1101 114-115 -0.017057964375066348 1101 116-117 0.1513296708006564 1101 118-119 0.08164637126505525 1101 120-121 -0.12969226983583582 1101 122-123 0.11262651587680494 1101 124-125 0.23427560229501765 1102 1 -0.055329462675192786 1102 2 0.012173667291591528 1102 3 -0.040818041930752 1102 4 -0.16784978819868712 1102 5 -0.07473396398565768 1102 6 0.07111400593733208 1102 7 -0.15242689694660072 1102 8 -0.12557447348594053 1102 9 -0.1490429540249849 1102 10-11 -0.1757235067397147 1102 12-13 0.09197382451132086 1102 14-15 0.11958187320846747 1102 16-17 0.13207875957199633 1102 18-19 0.20592809435619586 1102 20-21 0.16911970111861052 1102 22-23 0.17370566307890556 1102 24-25 0.16722195912892346 1102 26-27 0.2462495307673649 1102 28-29 0.19493437512424805 1102 30-31 0.013292870670831292 1102 32-33 0.22444507180092899 1102 34-35 0.28184588881627803 1102 36-37 0.09209375581460222 1102 38-39 -0.07424732687871938 1102 40-41 0.09805202401538082 1102 42-43 -0.07080155065612104 1102 44-45 -0.1460613737301273 1102 46-47 -0.17257138442754183 1102 48-49 -0.14508426957149823 1102 50-51 -0.2360081318426026 1102 52-53 -0.041450226910974663 1102 54-55 -0.04077654043299361 1102 56-57 0.0572955348354256 1102 58-59 -0.0036922670534629276 1102 60-61 -0.01973610641763557 1102 62-63 -0.004927190277474125 1102 64-65 -0.051981099425908894 1102 66-67 -0.1604753277347193 1102 68-69 0.0057760522369321166 1102 70-71 0.0027235137647600993 1102 72-73 -0.09936591397472228 1102 74-75 -4.2911419806657136E-4 1102 76-77 0.13359417463934165 1102 78-79 0.06693619606759782 1102 80-81 -0.1810088378561936 1102 82-83 -0.03602126478565992 1102 84-85 0.03128425104962318 1102 86-87 0.09319338978783875 1102 88-89 0.05106744989875622 1102 90-91 0.18456096846508174 1102 92-93 0.3717271510876614 1102 94-95 0.4335076845457806 1102 96-97 0.08664947132583123 1102 98-99 0.18917192025796226 1102 100-101 0.3856922863538834 1102 102-103 0.27098660337284386 1102 104-105 -0.03488035088221153 1102 106-107 -0.2654833957101985 1102 108-109 -0.15590063307654134 1102 110-111 -0.03805439277827105 1102 112-113 -0.067658912675995 1102 114-115 -0.17394422732302317 1102 116-117 -0.009969933468816805 1102 118-119 -0.19277382833448087 1102 120-121 -0.3388102322177886 1102 122-123 -0.14949292852384843 1102 124-125 -0.050135216441482555 1103 1 0.3497017760608401 1103 2 0.3314725979656288 1103 3 0.3015675161612812 1103 4 0.19651101920248948 1103 5 0.3110103589748192 1103 6 0.1570175263025817 1103 7 0.1568876530954526 1103 8 0.29501005132482305 1103 9 0.31585852380752755 1103 10-11 0.35275030078161507 1103 12-13 0.32142384253366885 1103 14-15 0.40566411518852163 1103 16-17 0.38420539660191366 1103 18-19 0.36024594849533287 1103 20-21 0.38857632733511593 1103 22-23 0.4091045577082397 1103 24-25 0.5165205894304989 1103 26-27 0.46706834609837955 1103 28-29 0.5038253999950868 1103 30-31 0.589591981568347 1103 32-33 0.6915888300611712 1103 34-35 0.5714620128100307 1103 36-37 0.6205900912705715 1103 38-39 0.6211719930780291 1103 40-41 0.6486632819752458 1103 42-43 0.5646799464002328 1103 44-45 0.6798127101338665 1103 46-47 0.7690752574083319 1103 48-49 0.5656330198674056 1103 50-51 0.6382754798358761 1103 52-53 0.6859467453346113 1103 54-55 0.8057992135018779 1103 56-57 0.8099448199489672 1103 58-59 0.7987137163659774 1103 60-61 0.7004594360550236 1103 62-63 0.35483942031966365 1103 64-65 0.48558498251570015 1103 66-67 0.6602578814446645 1103 68-69 0.7487203031080121 1103 70-71 0.7884918665221754 1103 72-73 0.760963894988393 1103 74-75 0.7776211681520486 1103 76-77 0.8483649904509463 1103 78-79 0.8865775513481466 1103 80-81 0.9036616535531508 1103 82-83 1.0268106467846891 1103 84-85 1.1810034027977885 1103 86-87 1.076342494677899 1103 88-89 1.0060326066931822 1103 90-91 0.9677716497098281 1103 92-93 1.037001090772872 1103 94-95 1.1603579789107954 1103 96-97 0.8895555748942598 1103 98-99 1.024636896828909 1103 100-101 1.1279336656642265 1103 102-103 1.1924271896998917 1103 104-105 1.1127527141183933 1103 106-107 1.0666309260487807 1103 108-109 1.0640576151512562 1103 110-111 1.2026806186358812 1103 112-113 1.1139718094194002 1103 114-115 0.974104853537245 1103 116-117 1.0418878702077379 1103 118-119 0.9156200801119923 1103 120-121 1.00695151234547 1103 122-123 1.0742800499694845 1103 124-125 0.9401657567652961 1104 1 0.32021468906040695 1104 2 0.3469140926994321 1104 3 0.3551276085000481 1104 4 0.41401570828054446 1104 5 0.4639536572001788 1104 6 0.38474440554078626 1104 7 0.4359851859190016 1104 8 0.3634958398113355 1104 9 0.4011961374274904 1104 10-11 0.5451197279926916 1104 12-13 0.4431324704372912 1104 14-15 0.6647843720057196 1104 16-17 0.602716433970258 1104 18-19 0.46969444077638656 1104 20-21 0.48165633022070153 1104 22-23 0.4094760769695327 1104 24-25 0.339206709759452 1104 26-27 0.474495124341054 1104 28-29 0.3842466956234247 1104 30-31 0.3662097134973621 1104 32-33 0.3898340327736278 1104 34-35 0.5240265778078665 1104 36-37 0.5014117620251497 1104 38-39 0.2352897250070427 1104 40-41 0.23356986111246414 1104 42-43 0.07591750230847083 1104 44-45 0.048789048324600515 1104 46-47 0.20079506695962834 1104 48-49 0.2811574752129573 1104 50-51 0.22120543222369804 1104 52-53 0.10176336631282368 1104 54-55 0.14599543338356824 1104 56-57 0.22929267740099135 1104 58-59 0.1958317368536413 1104 60-61 0.06945921963604107 1104 62-63 -0.05907812404622348 1104 64-65 0.14338869049434422 1104 66-67 0.06095943966137085 1104 68-69 0.2310424788402301 1104 70-71 0.18404084935293596 1104 72-73 0.07205536808029933 1104 74-75 0.17445243348172568 1104 76-77 0.1606377505144323 1104 78-79 0.07086553288038999 1104 80-81 0.10771049210458727 1104 82-83 0.19132153987370515 1104 84-85 0.13569067736134244 1104 86-87 0.08090532832384056 1104 88-89 0.18940080752999933 1104 90-91 0.18185150831608965 1104 92-93 0.08544648104844654 1104 94-95 0.19748104917482578 1104 96-97 -0.035669861550697846 1104 98-99 -0.07926946588931116 1104 100-101 0.03935337421999208 1104 102-103 0.04270492740007725 1104 104-105 0.10814298969189906 1104 106-107 0.1865649182577016 1104 108-109 0.23131161890252372 1104 110-111 0.04767592955782618 1104 112-113 -0.024318119883727007 1104 114-115 -0.14182178042610616 1104 116-117 -0.23160260735682314 1104 118-119 -0.063894059261834 1104 120-121 0.04022953860430789 1104 122-123 -0.1480561929968971 1104 124-125 0.032829153100600195 1105 1 0.30353303894312944 1105 2 0.3295409507036382 1105 3 0.3180303873105217 1105 4 0.4431671943459392 1105 5 0.4381704057688651 1105 6 0.4938608776479114 1105 7 0.4324168715233938 1105 8 0.49444601589504344 1105 9 0.4613309208884644 1105 10-11 0.6110068045975581 1105 12-13 0.5513560190140936 1105 14-15 0.7019045753300119 1105 16-17 0.8065551264219835 1105 18-19 0.7843330467071326 1105 20-21 0.5869489806691917 1105 22-23 0.7072035494078719 1105 24-25 0.7771961785341688 1105 26-27 0.7229245658075882 1105 28-29 0.737625745379745 1105 30-31 0.7255725676081184 1105 32-33 0.7443567817073173 1105 34-35 0.7682435291600882 1105 36-37 0.6966318159655742 1105 38-39 0.8081420783100484 1105 40-41 0.8232684571744073 1105 42-43 0.799908485200298 1105 44-45 0.9164220912268419 1105 46-47 0.8230471809780511 1105 48-49 0.7436398161461639 1105 50-51 0.8072208032161896 1105 52-53 0.9180438429893414 1105 54-55 0.994529646346848 1105 56-57 0.9983688631777596 1105 58-59 0.9881753062495484 1105 60-61 0.8814765103127336 1105 62-63 0.9300239504294083 1105 64-65 0.8547837736693928 1105 66-67 0.9020278385501186 1105 68-69 0.9436718378414533 1105 70-71 0.8174930363734383 1105 72-73 0.859333066622284 1105 74-75 0.9513378134642885 1105 76-77 0.9052065313121815 1105 78-79 0.9507243955350972 1105 80-81 0.992766020997859 1105 82-83 0.8100107470576532 1105 84-85 0.8340359636579073 1105 86-87 0.8772379879651844 1105 88-89 1.0124542545122424 1105 90-91 1.0655649481333107 1105 92-93 0.9750254348205551 1105 94-95 1.0436430326682498 1105 96-97 1.156546968131405 1105 98-99 1.1597254712815896 1105 100-101 1.2227278275493596 1105 102-103 1.086239512133183 1105 104-105 0.9185991850670874 1105 106-107 1.0827755698012211 1105 108-109 1.0439271210283678 1105 110-111 1.0824178199060057 1105 112-113 1.329099796718161 1105 114-115 1.348694709255593 1105 116-117 1.132932240994883 1105 118-119 1.0627003263378292 1105 120-121 1.3460663248907316 1105 122-123 1.2403091012758196 1105 124-125 0.8039588602135694 1106 1 0.012361736982214211 1106 2 0.03043715200145769 1106 3 0.18181836240615468 1106 4 -0.6482869828340014 1106 5 -0.1116015792699514 1106 6 0.005208763884112955 1106 7 0.062291924224055606 1106 8 0.10322275462999642 1106 9 0.31843509864859243 1106 10-11 0.2800898582574618 1106 12-13 0.27746279731805146 1106 14-15 0.3131052220492947 1106 16-17 0.3254828823602338 1106 18-19 0.30854279468516665 1106 20-21 0.3552607674374926 1106 22-23 0.3816456315448278 1106 24-25 0.3851039204704847 1106 26-27 0.31992149853253693 1106 28-29 0.31404363485211917 1106 30-31 0.26084752000024736 1106 32-33 0.43255293001438844 1106 34-35 0.3587442595557704 1106 36-37 0.4306714133389846 1106 38-39 0.4629682338243768 1106 40-41 0.3847274177046742 1106 42-43 0.21292725630473086 1106 44-45 -0.018370271400108606 1106 46-47 0.09046805263083257 1106 48-49 0.013160333624732345 1106 50-51 -0.06992380739202275 1106 52-53 0.12381654746467063 1106 54-55 0.162331123125675 1106 56-57 0.12344896173281938 1106 58-59 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38-39 -1.7195657775715318 2216 40-41 -1.6665031173832077 2216 42-43 -1.6486184403161275 2216 44-45 -1.5422437547937236 2216 46-47 -1.667306175678135 2216 48-49 -1.7788242658536149 2216 50-51 -1.865279522758584 2216 52-53 -1.713641678647651 2216 54-55 -1.6785585392223048 2216 56-57 -1.6161554678004535 2216 58-59 -1.8774750189517277 2216 60-61 -1.8358541872290886 2216 62-63 -1.8827549237531684 2216 64-65 -1.6681421980116866 2216 66-67 -1.7726581759165292 2216 68-69 -1.9016558938824133 2216 70-71 -1.7299335309022332 2216 72-73 -1.7784282933223778 2216 74-75 -1.816204601931446 2216 76-77 -2.046004437352252 2216 78-79 -2.5276165144096723 2216 80-81 -2.509076565230753 2216 82-83 -2.8125320108878427 2216 84-85 -2.755725334406794 2216 86-87 -2.7578536938215557 2216 88-89 -2.6745077489839737 2216 90-91 -2.9034657907354777 2216 92-93 -2.6261422178737632 2216 94-95 -2.7138389171692054 2216 96-97 -3.026579030511659 2216 98-99 -2.894402916369824 2216 100-101 -2.912016331977167 2216 102-103 -2.969635879312797 2216 104-105 -3.3489553853202594 2216 106-107 -2.823490659875084 2216 108-109 -3.4334546495782092 2216 110-111 -3.598797635055327 2216 112-113 -3.5899358041472773 2216 114-115 -3.4989899858419697 2216 116-117 -3.6967183140462367 2216 118-119 -3.628455701764512 2216 120-121 -3.065254723797743 2216 122-123 -2.73829424598787 2216 124-125 -2.547956324464952 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 33095.0 3 3997.0 4 2999.0 5 4161.0 6 7082.0 7 6679.0 8 5068.0 9 4824.0 10 4188.0 11 3724.0 12 3597.0 13 3364.0 14 3135.0 15 3253.0 16 3438.0 17 3472.0 18 3656.0 19 4327.0 20 4888.0 21 5850.0 22 6479.0 23 7592.0 24 8851.0 25 9699.0 26 10880.0 27 12262.0 28 14543.0 29 17628.0 30 22584.0 31 29576.0 32 39673.0 33 54287.0 34 80361.0 35 124481.0 36 218669.0 37 89160.0 38 4586.0 39 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 55.98647086031452 17.278792784458837 10.507978723404255 16.226757631822387 2 17.511906026689825 23.83861386595962 34.435785982530135 24.213694124820428 3 33.500416263970244 21.843754943495487 23.54010279006555 21.115726002468715 4 16.906668391137757 20.108410722822835 29.127240561407724 33.85768032463169 5 17.054874000519586 32.68365903645758 25.968767139104582 24.29269982391825 6 33.94018325574522 34.1415428951452 9.332164893223972 22.586108955885614 7 29.142800403181578 37.887753417287165 12.060925216282902 20.90852096324836 8 38.08914623515625 26.108035399864914 12.172427130659678 23.630391234319166 9 37.647440515776246 15.698893445651372 15.555147602157687 31.09851843641469 10-11 29.834651869504174 27.200639178304886 15.452620446316413 27.512088505874527 12-13 32.77509144313899 27.64007426291288 17.660360322914357 21.92447397103377 14-15 36.829243792950564 20.634456051873197 21.04179367102638 21.494506484149856 16-17 30.663124399615754 22.954017494273256 23.28497930983522 23.09787879627577 18-19 30.728993478903423 23.18580045075002 22.997545352102268 23.08766071824429 20-21 36.64422153255006 22.054884356757558 18.761257204610953 22.53963690608143 22-23 32.097119079287665 25.62405324022759 17.67132888125323 24.607498799231507 24-25 27.89275520948792 27.44240929579546 16.737385003325205 27.927450491391415 26-27 26.377694107975607 24.94905340357902 19.78869718728604 28.88455530115933 28-29 28.62838694480432 21.789489241910935 24.188495761195135 25.39362805208961 30-31 30.534965454814156 24.58896770856425 20.752166001625653 24.123900834995936 32-33 25.92776684770561 23.12420333628907 24.000302501293135 26.947727314712182 34-35 23.28521022685288 24.16298354023498 23.526114405527228 29.025691827384907 36-37 26.17484805660238 27.371113666592773 18.471052242666076 27.982986034138772 38-39 28.519406266164193 22.29417211630828 20.844475079435455 28.34194653809207 40-41 25.380608974358974 23.633779465011454 22.23950251237715 28.74610904825242 42-43 26.219819145791767 23.397147251163823 23.532464623512894 26.850568979531513 44-45 27.336822489470187 20.933320401980342 25.610544594694453 26.11931251385502 46-47 24.308172615089042 22.297809059336437 25.975970775142244 27.418047550432277 48-49 23.937897177270376 22.85160579694081 26.222474691494863 26.988022334293948 50-51 24.678852157688613 23.506832834552576 24.529622035025493 27.28469297273332 52-53 23.109078271632306 24.521193563880882 23.674247672356465 28.695480492130347 54-55 21.620760363555753 24.52419548511047 22.853799508608585 31.00124464272519 56-57 22.22142683541439 22.47468697520106 24.273544042844666 31.030342146539887 58-59 23.994183200325132 20.794135169585456 25.778709912805738 29.432971717283678 60-61 24.865505191883493 21.74494854299937 24.3114194881375 29.07812677697964 62-63 23.058218798492575 21.01806694746176 25.53474608364738 30.388968170398282 64-65 21.977484007248623 21.427948765465242 25.14855463398667 31.446012593299464 66-67 23.8766274664202 23.16978761898769 22.158655641873967 30.794929272718143 68-69 24.738341028138333 21.67925912797085 22.96843003462229 30.61396980926852 70-71 23.807893332840617 22.01725049829897 23.667873054439884 30.506983114420528 72-73 25.066027949089637 21.6278407893681 24.05397463718544 29.25215662435682 74-75 26.111312860370205 19.73323295265269 25.546902468891325 28.608551718085785 76-77 24.69526081863783 20.173834832662614 26.190683741489963 28.9402206072096 78-79 25.03473904009985 20.893360528021866 26.388738951195617 27.683161480682667 80-81 26.23282530885579 21.492495092945386 25.377900935226876 26.896778662971943 82-83 24.987732519272935 22.01755895029621 25.461228410677155 27.533480119753705 84-85 24.360159526340496 22.013341594120135 25.478426008313527 28.148072871225843 86-87 24.83738764925496 20.84676769886854 25.869089191871314 28.446755460005186 88-89 25.424925702599065 19.769546697800266 26.93645269823563 27.86907490136504 90-91 26.595205464426613 20.074851837697476 26.15138244405163 27.17856025382428 92-93 26.595706598304808 19.89705304972401 26.36331093096838 27.143929421002795 94-95 25.527431032690906 20.417094885622237 26.114216099493063 27.94125798219379 96-97 25.95570745675819 21.014594831766853 24.707641502898184 28.322056208576775 98-99 26.04973273547364 20.599372235779327 24.95440713690675 28.396487891840277 100-101 25.454217969204624 21.535808632681526 25.03290597895865 27.977067419155205 102-103 25.808282189995168 22.279925852502824 25.34858387170705 26.563208085794958 104-105 25.516695137024296 22.860494477866727 25.78548456279287 25.837325822316103 106-107 24.472189460473164 23.826999491399103 25.756276833604375 25.944534214523358 108-109 24.094112539717727 25.21608059927584 25.827664320549765 24.86214254045666 110-111 24.177935417128502 27.27684179413286 24.44995566393261 24.09526712480603 112-113 23.696760714535188 28.862552483744686 23.67551609135881 23.765170710361314 114-115 23.19281438370281 29.861551722246695 23.582393207888337 23.363240686162158 116-117 23.070341941919754 30.155395607034656 23.401246028227295 23.373016422818296 118-119 23.189777239348548 30.237018627224995 23.78880864444664 22.784395488979822 120-121 23.70168913048373 30.40348275278794 23.475444133534772 22.41938398319356 122-123 24.159781634152917 30.73050142049801 23.096356585701265 22.01336035964781 124-125 24.794825588729903 31.907789278269856 21.941538457977295 21.355846675022946 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5.0 1 6.0 2 8.0 3 7.5 4 8.5 5 12.0 6 13.5 7 14.5 8 15.0 9 14.5 10 18.0 11 18.0 12 20.5 13 26.0 14 24.0 15 30.5 16 37.5 17 46.5 18 65.0 19 93.5 20 127.0 21 153.5 22 202.0 23 290.5 24 409.5 25 529.5 26 662.5 27 817.5 28 960.5 29 1123.0 30 1308.5 31 1477.0 32 1616.5 33 1730.0 34 1795.5 35 1849.5 36 1928.0 37 1950.5 38 1934.5 39 1938.5 40 1924.5 41 1894.5 42 1871.0 43 1889.5 44 1960.5 45 2335.0 46 3332.0 47 4851.5 48 7496.0 49 13093.0 50 23732.0 51 40726.0 52 64371.0 53 89580.0 54 106693.5 55 109627.0 56 99611.5 57 80922.5 58 59367.5 59 39886.5 60 25072.0 61 16027.5 62 10667.0 63 7632.0 64 5877.5 65 4703.0 66 3849.0 67 2957.0 68 2355.0 69 1909.0 70 1478.5 71 1176.5 72 885.0 73 658.0 74 539.0 75 431.5 76 312.5 77 219.0 78 165.5 79 134.0 80 123.0 81 99.0 82 78.5 83 65.0 84 47.5 85 36.0 86 28.0 87 27.5 88 27.5 89 19.5 90 12.0 91 13.0 92 11.0 93 6.5 94 5.0 95 3.0 96 2.5 97 3.0 98 2.0 99 1.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15148156358530998 2 0.018935195448163748 3 0.008659388162270007 4 0.016856942289218944 5 0.00427196482671987 6 0.0 7 1.1545850883026676E-4 8 8.082095618118673E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0010391265794724009 28-29 0.007620261582797606 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 7.504803073967339E-4 58-59 0.0 60-61 5.772925441513338E-5 62-63 0.0 64-65 0.0019050653956994015 66-67 4.0410478090593363E-4 68-69 0.005946113204758738 70-71 0.01916611246582428 72-73 1.7318776324540012E-4 74-75 0.00871711741668514 76-77 2.886462720756669E-4 78-79 0.0013277728515480678 80-81 0.001385502105963201 82-83 1.1545850883026676E-4 84-85 0.02003205128205128 86-87 0.002135982413359935 88-89 0.019223841720239415 90-91 1.1545850883026676E-4 92-93 0.015240523165595212 94-95 0.014085938077292543 96-97 0.006003842459173871 98-99 0.0025978164486810023 100-101 9.236680706421341E-4 102-103 5.772925441513338E-5 104-105 7.504803073967339E-4 106-107 0.001212314342717801 108-109 0.0 110-111 0.0 112-113 0.001212314342717801 114-115 0.005599737678267938 116-117 0.0 118-119 0.07291204832631346 120-121 0.0017896068868691348 122-123 0.004906986625286337 124-125 0.24142374196408778 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 866112.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.61024349998515 #Duplication Level Percentage of deduplicated Percentage of total 1 81.70118507236216 51.15331091617947 2 9.757480402853705 12.218364479380075 3 3.2257186141840104 6.058890836894867 4 1.5686328023481984 3.9284992686833915 5 0.9116568523744829 2.85395287577982 6 0.6195432012732704 2.3273850414287867 7 0.4336307238489578 1.9004807646480648 8 0.3212041611404109 1.6088536593767675 9 0.23666028147536627 1.333562206495294 >10 1.1695487128614914 12.535833349229557 >50 0.03852160578392952 1.6660941780629595 >100 0.015111826153025377 1.861387084226803 >500 9.214527842527498E-4 0.4311928720054387 >1k 1.8429055685054995E-4 0.12219246760871276 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT 1059 0.1222705608512525 No Hit ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA 946 0.10922374935343235 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 1.1545850883026676E-4 0.0 0.0 0.0 3 0.0 1.1545850883026676E-4 0.0 0.0 0.0 4 0.0 1.1545850883026676E-4 0.0 0.0 0.0 5 0.0 1.1545850883026676E-4 0.0 0.0 0.0 6 0.0 1.1545850883026676E-4 0.0 0.0 0.0 7 0.0 1.1545850883026676E-4 0.0 0.0 0.0 8 1.1545850883026676E-4 1.1545850883026676E-4 0.0 0.0 0.0 9 1.1545850883026676E-4 1.1545850883026676E-4 0.0 0.0 0.0 10-11 2.886462720756669E-4 1.1545850883026676E-4 0.0 0.0 0.0 12-13 3.463755264908003E-4 1.1545850883026676E-4 0.0 0.0 0.0 14-15 4.6183403532106703E-4 1.1545850883026676E-4 0.0 0.0 0.0 16-17 6.350217985664672E-4 1.1545850883026676E-4 0.0 3.463755264908003E-4 0.0 18-19 0.0010391265794724006 1.1545850883026676E-4 0.0 8.659388162270007E-4 0.0 20-21 0.0013277728515480678 1.1545850883026676E-4 1.1545850883026676E-4 0.0031751089928323357 0.0 22-23 0.0015586898692086012 1.1545850883026676E-4 1.1545850883026676E-4 0.011257204610951008 0.0 24-25 0.0016741483780388679 1.1545850883026676E-4 1.1545850883026676E-4 0.024708120889677086 0.0 26-27 0.001962794650114535 1.1545850883026676E-4 2.3091701766053352E-4 0.06615772555974285 0.0 28-29 0.003406026010492869 1.1545850883026676E-4 2.3091701766053352E-4 0.2459266238084682 0.0 30-31 0.005080174388531738 1.1545850883026676E-4 2.886462720756669E-4 0.6547652035764429 0.0 32-33 0.005542008423852805 1.1545850883026676E-4 3.463755264908003E-4 1.272064121037464 0.0 34-35 0.005946113204758738 1.1545850883026676E-4 4.0410478090593363E-4 2.1785288738638884 0.0 36-37 0.006465676494494938 1.1545850883026676E-4 4.6183403532106703E-4 3.4146853986551395 0.0 38-39 0.006638864257740338 1.1545850883026676E-4 4.6183403532106703E-4 5.16971246212961 0.0 40-41 0.00750480307396734 1.1545850883026676E-4 5.195632897362004E-4 7.400717228256854 0.0 42-43 0.00889030517993054 1.1545850883026676E-4 5.772925441513338E-4 9.168502456957068 0.0 44-45 0.00923668070642134 1.1545850883026676E-4 5.772925441513338E-4 10.696595821325648 0.0 46-47 0.00923668070642134 1.1545850883026676E-4 6.927510529816006E-4 12.506927510529817 0.0 48-49 0.00923668070642134 1.1545850883026676E-4 9.236680706421341E-4 14.368638236163452 0.0 50-51 0.00923668070642134 1.1545850883026676E-4 0.0010391265794724009 16.272260400502475 0.0 52-53 0.009352139215251607 1.1545850883026676E-4 0.0013855021059632011 18.156254618340355 0.0 54-55 0.009352139215251607 1.1545850883026676E-4 0.0013855021059632011 19.85285967634671 0.0 56-57 0.009352139215251607 1.1545850883026676E-4 0.0013855021059632011 21.540805346190794 0.0 58-59 0.009352139215251607 1.1545850883026676E-4 0.0013855021059632011 23.28826987733688 0.0 60-61 0.009352139215251607 1.1545850883026676E-4 0.0014432313603783345 25.103046719131015 0.0 62-63 0.009352139215251607 1.1545850883026676E-4 0.0017318776324540012 26.868349589891377 0.0 64-65 0.009352139215251607 1.1545850883026676E-4 0.0018473361412842681 28.578982856720607 0.0 66-67 0.009352139215251607 1.1545850883026676E-4 0.0021937116677750684 30.314151056676273 0.0 68-69 0.009352139215251607 1.1545850883026676E-4 0.0025400871942658685 32.212288941845856 0.0 70-71 0.009352139215251607 1.1545850883026676E-4 0.002655545703096135 34.39797624325722 0.0 72-73 0.009352139215251607 1.1545850883026676E-4 0.002713274957511269 36.500187042784304 0.0 74-75 0.009352139215251607 1.1545850883026676E-4 0.0028287334663415356 38.34850458139363 0.0 76-77 0.009352139215251607 1.1545850883026676E-4 0.002886462720756669 40.212120372422966 0.0 78-79 0.009352139215251607 1.1545850883026676E-4 0.002886462720756669 42.04744882878889 0.0 80-81 0.009352139215251607 1.1545850883026676E-4 0.002886462720756669 43.953668809576584 0.0 82-83 0.009352139215251607 1.1545850883026676E-4 0.002886462720756669 46.04508423852804 0.0 84-85 0.009352139215251607 1.7318776324540014E-4 0.0030019212295869357 48.11739128426808 0.0 86-87 0.009352139215251607 2.3091701766053352E-4 0.0031173797384172024 49.8817127577034 0.0 88-89 0.009352139215251607 2.3091701766053352E-4 0.003406026010492869 51.54535441143871 0.0 90-91 0.009352139215251607 2.3091701766053352E-4 0.0036946722825685363 53.336173612650555 0.0 92-93 0.009352139215251607 2.3091701766053352E-4 0.0036946722825685363 55.04686460873421 0.0 94-95 0.009352139215251607 2.3091701766053352E-4 0.0036946722825685363 56.676446002364585 0.0 96-97 0.009352139215251607 2.3091701766053352E-4 0.003810130791398803 58.23317307692308 0.0 98-99 0.009352139215251607 2.3091701766053352E-4 0.003810130791398803 59.72582067908077 0.0 100-101 0.009352139215251607 2.3091701766053352E-4 0.003810130791398803 61.32555604817853 0.0 102-103 0.009352139215251607 2.3091701766053352E-4 0.003810130791398803 62.86230880070938 0.0 104-105 0.009352139215251607 2.3091701766053352E-4 0.004041047809059337 64.28885640656173 0.0 106-107 0.009352139215251607 2.3091701766053352E-4 0.004041047809059337 65.64861126505579 0.0 108-109 0.009352139215251607 2.3091701766053352E-4 0.00427196482671987 66.88072674203798 0.0 110-111 0.009409868469666741 2.3091701766053352E-4 0.00427196482671987 68.151058985443 0.0 112-113 0.009467597724081874 2.3091701766053352E-4 0.00427196482671987 69.4796977758073 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAACTTA 25 0.0020584464 71.384514 6 CTAGTCG 15 0.004240554 59.4871 36-37 GCTAGTC 15 0.004240554 59.4871 34-35 TATAAGG 160 0.0 48.35001 2 TCGGAGA 55 8.524234E-4 43.275837 4 TAACAAC 180 0.0 43.020027 1 TCGATTG 30 0.0013100917 39.658066 58-59 GATTGCG 30 0.0013100917 39.658066 60-61 GTATAGG 570 0.0 37.62075 1 TAAGGGT 80 1.1967205E-4 37.19017 4 TAGCTCA 40 1.1992513E-4 37.179436 14-15 GGCATAT 65 0.0019407012 36.607445 8 GTAACAA 105 1.4720157E-5 34.03782 1 ATTCCTA 35 0.002792342 33.992626 36-37 GTATAAG 215 3.6379788E-12 33.246246 1 TATATGG 235 0.0 32.91916 2 GTCCAAG 275 0.0 32.49065 1 GGTAAGG 130 1.7383645E-6 32.031513 8 CACCGAT 75 0.003914112 31.726454 7 TAAGATG 95 3.2842692E-4 31.318037 4 >>END_MODULE