FastQCFastQC Report
Thu 26 May 2016
SRR935993_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935993_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences866112
Sequences flagged as poor quality0
Sequence length125
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT72480.8368432720017736No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT39910.4607949087415946TruSeq Adapter, Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT23230.26821011601270967RNA PCR Primer, Index 10 (95% over 23bp)
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCT10250.11834497155102343TruSeq Adapter, Index 10 (96% over 26bp)
ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA9800.11314933865366142No Hit
GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT9000.10391265794724008No Hit
GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT8740.10091073671765313No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACA401.7422375E-459.702381
ATTCTTA150.004238159459.49558328-29
TATAACA350.00774995351.075833
TATGATA602.1737496E-549.657054
TAGGATT255.33531E-447.59646614-15
CTTTACA1301.8189894E-1145.9249081
ATGTACA853.4445202E-642.0624474
TACAGGT853.4816676E-641.9968837
GTCTTAG758.024779E-539.8015861
CAAGGTA600.001298811339.7256434
CAGATGT905.148464E-639.6728825
TAGGGAG1950.039.6728825
AATTGAG300.001309173439.6637266-67
TGGATCG300.001309173439.6637238-39
GCTCGGA650.001924546336.6698233
TATACGG2850.035.5439952
GTCAGAT1051.4682513E-534.0505523
GATGTGT1408.050665E-834.005335
ACGGGCG700.002786466634.005335
TTTTAGC350.0027903933.99747580-81
Produced by FastQC (version 0.11.5)