##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935993_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 866112 Sequences flagged as poor quality 0 Sequence length 125 %GC 53 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.35134832446612 33.0 31.0 34.0 31.0 34.0 2 32.45354757814232 34.0 31.0 34.0 31.0 34.0 3 32.466847243774474 34.0 31.0 34.0 30.0 34.0 4 35.92494157799453 37.0 35.0 37.0 35.0 37.0 5 35.909689508978055 37.0 35.0 37.0 35.0 37.0 6 35.98543837286633 37.0 35.0 37.0 35.0 37.0 7 35.964445706790805 37.0 35.0 37.0 35.0 37.0 8 35.992875055420086 37.0 35.0 37.0 35.0 37.0 9 37.691967089706644 39.0 37.0 39.0 35.0 39.0 10-11 37.599463464309466 39.0 37.0 39.0 35.0 39.0 12-13 37.57491929450232 39.0 37.0 39.0 35.0 39.0 14-15 38.72524165465898 40.0 38.0 41.0 34.0 41.0 16-17 38.63294874104042 40.0 38.0 41.0 34.0 41.0 18-19 38.66265563806991 40.0 38.0 41.0 34.0 41.0 20-21 38.730470193231355 40.0 38.0 41.0 34.0 41.0 22-23 38.592850578216215 40.0 38.0 41.0 34.0 41.0 24-25 38.36631174721052 40.0 38.0 41.0 34.0 41.0 26-27 38.14348028892337 40.0 38.0 41.0 33.0 41.0 28-29 38.05745042211631 40.0 37.0 41.0 33.0 41.0 30-31 37.915478598610804 40.0 37.0 41.0 33.0 41.0 32-33 37.57555778005616 40.0 36.5 41.0 32.0 41.0 34-35 37.56394380865292 40.0 36.0 41.0 33.0 41.0 36-37 37.641602933569786 40.0 37.0 41.0 33.0 41.0 38-39 37.363096227739604 40.0 36.0 41.0 32.0 41.0 40-41 37.1683067547846 40.0 36.0 41.0 32.0 41.0 42-43 36.97671432793911 39.5 35.0 41.0 31.0 41.0 44-45 36.630836427621375 39.0 35.0 41.0 31.0 41.0 46-47 36.22547545813936 39.0 35.0 41.0 31.0 41.0 48-49 35.74907806380699 38.5 35.0 40.5 29.5 41.0 50-51 35.26792262432572 37.0 35.0 40.0 29.0 41.0 52-53 34.84414948644056 37.0 34.5 39.5 28.5 41.0 54-55 34.395612230288926 36.0 34.0 39.0 28.0 41.0 56-57 33.97452581190423 35.0 34.0 39.0 28.0 41.0 58-59 33.633040530554936 35.0 34.0 37.5 27.0 40.5 60-61 33.2251412057563 35.0 33.5 37.0 26.5 39.5 62-63 32.79208635834627 35.0 33.0 36.0 26.0 39.0 64-65 32.522434165558266 35.0 33.0 36.0 26.0 38.5 66-67 32.17854676993276 35.0 33.0 35.0 25.5 37.0 68-69 31.781785150188426 35.0 33.0 35.0 24.5 36.5 70-71 31.389553545038055 35.0 33.0 35.0 23.5 36.0 72-73 31.125241308283456 35.0 33.0 35.0 20.0 35.5 74-75 30.934485378334443 35.0 32.5 35.0 20.0 35.0 76-77 30.771699849442104 35.0 32.0 35.0 19.5 35.0 78-79 30.851256534951602 35.0 32.5 35.0 20.0 35.0 80-81 30.827958739747285 35.0 33.0 35.0 19.5 35.0 82-83 30.713613828234685 35.0 32.5 35.0 19.0 35.0 84-85 30.632993192566317 35.0 32.0 35.0 19.0 35.0 86-87 30.61664080396069 35.0 32.0 35.0 19.0 35.0 88-89 30.55084561811867 35.0 32.0 35.0 18.0 35.0 90-91 30.434962799268455 35.0 32.0 35.0 17.5 35.0 92-93 30.32383918015222 35.0 32.0 35.0 16.5 35.0 94-95 30.288318947203134 35.0 32.0 35.0 15.0 35.0 96-97 30.26324020450011 35.0 32.0 35.0 15.0 35.0 98-99 30.192765485295205 35.0 32.0 35.0 13.0 35.0 100-101 30.0914748900835 35.0 31.0 35.0 9.0 35.0 102-103 29.247893459506393 33.5 29.5 35.0 7.0 35.0 104-105 29.777009786263207 34.5 31.0 35.0 4.5 35.0 106-107 29.67493003214365 35.0 31.0 35.0 2.0 35.0 108-109 29.42839551928619 35.0 31.0 35.0 2.0 35.0 110-111 29.08161126967413 35.0 31.0 35.0 2.0 35.0 112-113 28.60475435047661 35.0 30.0 35.0 2.0 35.0 114-115 27.973555960430062 34.0 29.0 35.0 2.0 35.0 116-117 27.29520373808468 34.0 28.0 35.0 2.0 35.0 118-119 26.716786628057342 34.0 25.0 35.0 2.0 35.0 120-121 26.078067270745585 34.0 22.0 35.0 2.0 35.0 122-123 25.32442166832927 34.0 8.5 35.0 2.0 35.0 124-125 22.778528065654328 30.5 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.042604297657064194 1101 2 0.18494340911068718 1101 3 0.12138897721670361 1101 4 0.1973772495395778 1101 5 0.18111372561183714 1101 6 0.1764921734590459 1101 7 0.19020779233617446 1101 8 0.226108038207812 1101 9 0.2886347476012787 1101 10-11 0.24918011259862283 1101 12-13 0.3663582310739386 1101 14-15 0.34002864703828095 1101 16-17 0.25681451944519296 1101 18-19 0.2954328737954057 1101 20-21 0.35419575151850324 1101 22-23 0.3374882558300669 1101 24-25 0.32916069422257976 1101 26-27 0.2388683351248133 1101 28-29 0.37813279327131966 1101 30-31 0.27994340298029385 1101 32-33 0.3104418250038776 1101 34-35 0.5037155303521885 1101 36-37 0.24978838004893333 1101 38-39 0.30624633911243393 1101 40-41 0.27148163381451695 1101 42-43 0.35330563384769675 1101 44-45 0.301562148562482 1101 46-47 0.3531935687600054 1101 48-49 0.35203458018733613 1101 50-51 0.3901318619217662 1101 52-53 0.48989738762205803 1101 54-55 0.4905497959786942 1101 56-57 0.25655904599437207 1101 58-59 0.22892525521368867 1101 60-61 0.1443837622687809 1101 62-63 0.27544771730129725 1101 64-65 0.20292459571645338 1101 66-67 0.09387524276821324 1101 68-69 0.2676891214560655 1101 70-71 0.4266033135661509 1101 72-73 0.5386478175776688 1101 74-75 0.5674154500344244 1101 76-77 0.5439156307708011 1101 78-79 0.4769699356776229 1101 80-81 0.4402149716068493 1101 82-83 0.43619129785831134 1101 84-85 0.5036211353649058 1101 86-87 0.4798878863687257 1101 88-89 0.3364692800358675 1101 90-91 0.42118215760516264 1101 92-93 0.4914933609082297 1101 94-95 0.39783677402756723 1101 96-97 0.5263485828349594 1101 98-99 0.34089072060214676 1101 100-101 0.2859133459769616 1101 102-103 0.40201023459002627 1101 104-105 0.5864521663762439 1101 106-107 0.678388138170984 1101 108-109 0.38137589012137596 1101 110-111 0.4328022469909989 1101 112-113 0.522615131374188 1101 114-115 0.6562375606708528 1101 116-117 0.5223115577613875 1101 118-119 0.3080065919428243 1101 120-121 0.8073982780301847 1101 122-123 0.8013782183370104 1101 124-125 0.7029825280953723 1102 1 0.004736354982803448 1102 2 0.038552282303491836 1102 3 0.171848560674853 1102 4 0.23497559093992493 1102 5 0.21865452054144185 1102 6 0.1731631495417858 1102 7 0.25868020944560755 1102 8 0.1668399031864496 1102 9 0.27865634723550414 1102 10-11 0.30227579740162724 1102 12-13 0.3485893786924734 1102 14-15 0.35382324555311584 1102 16-17 0.31167759037713694 1102 18-19 0.18094180532298765 1102 20-21 0.3136613576483853 1102 22-23 0.42703174677240696 1102 24-25 0.3840430786960667 1102 26-27 0.21983030715564666 1102 28-29 0.29799854444983964 1102 30-31 0.3634137704893945 1102 32-33 0.4153139614757322 1102 34-35 0.33318168829754313 1102 36-37 0.49594498528014697 1102 38-39 0.46708872484721553 1102 40-41 0.2804738610994306 1102 42-43 0.286022773236283 1102 44-45 0.3371223200982669 1102 46-47 0.34431288703140694 1102 48-49 0.316457065879554 1102 50-51 0.27566207776044394 1102 52-53 0.345400170348654 1102 54-55 0.4192706088817104 1102 56-57 0.4658055025507224 1102 58-59 0.4760581796780343 1102 60-61 0.38042480157380254 1102 62-63 0.5504985631555783 1102 64-65 0.49389334719452904 1102 66-67 0.3240004260497784 1102 68-69 0.2789654706448914 1102 70-71 0.42979162453781683 1102 72-73 0.35108022018224005 1102 74-75 0.24022986660780532 1102 76-77 0.18713933676351857 1102 78-79 0.24198719961884763 1102 80-81 0.3960295300756407 1102 82-83 0.31244983129044357 1102 84-85 0.4247623685936581 1102 86-87 0.4512943851783824 1102 88-89 0.5831276431935137 1102 90-91 0.45298210084699875 1102 92-93 0.47682452902275685 1102 94-95 0.4776060363291066 1102 96-97 0.5092513687147893 1102 98-99 0.5056466605015615 1102 100-101 0.3283203650697786 1102 102-103 0.268849672053566 1102 104-105 0.12023667455099485 1102 106-107 0.361076504324199 1102 108-109 0.20902972839214584 1102 110-111 0.2595605675800243 1102 112-113 0.07351695551848181 1102 114-115 0.2525372437711084 1102 116-117 0.5754852850384147 1102 118-119 0.7972670803783473 1102 120-121 0.9272975322909893 1102 122-123 0.7971647130474651 1102 124-125 0.5269565454696767 1103 1 0.07678669740741384 1103 2 0.14021334201753888 1103 3 0.10643344593257353 1103 4 0.20904443630818292 1103 5 0.06622450732505314 1103 6 0.15321241062552815 1103 7 0.23205519442698375 1103 8 0.1771066339325742 1103 9 0.3219940860316086 1103 10-11 0.17471061657740705 1103 12-13 0.09798563004414262 1103 14-15 0.03242020578380078 1103 16-17 0.225309994558323 1103 18-19 0.2805378043617921 1103 20-21 0.25480037002373024 1103 22-23 0.38269226581601856 1103 24-25 0.3346393180328491 1103 26-27 0.31369670148460926 1103 28-29 0.377828533636432 1103 30-31 0.30536078478712625 1103 32-33 0.2635117267046141 1103 34-35 0.4440221304458092 1103 36-37 0.4672969617309448 1103 38-39 0.45467433099884147 1103 40-41 0.3436301749285775 1103 42-43 0.4092250440520928 1103 44-45 0.340952068986887 1103 46-47 0.27160322705302775 1103 48-49 0.33426464728049154 1103 50-51 0.2600472153310349 1103 52-53 0.18045363664686676 1103 54-55 0.14965027967350153 1103 56-57 0.1898052622527544 1103 58-59 0.35222062110540975 1103 60-61 0.11440443632088915 1103 62-63 0.1893051833646453 1103 64-65 0.25345119893067647 1103 66-67 0.3358981792637721 1103 68-69 0.12811391471554145 1103 70-71 0.20949846101504832 1103 72-73 0.11179300403421522 1103 74-75 0.13257187069287113 1103 76-77 0.29409896670464875 1103 78-79 0.22640237443561873 1103 80-81 0.28566668014172336 1103 82-83 0.23075753284036793 1103 84-85 0.27980111664123797 1103 86-87 0.3767224159365199 1103 88-89 0.28330185922138895 1103 90-91 0.16733990452355485 1103 92-93 0.23818161180540187 1103 94-95 0.4019091722176107 1103 96-97 0.48914894170529877 1103 98-99 0.4187714352222116 1103 100-101 0.4715604828157822 1103 102-103 0.544436900216489 1103 104-105 0.31751980563353754 1103 106-107 0.42835693205458725 1103 108-109 0.49713497890277836 1103 110-111 0.5549858949860074 1103 112-113 0.6164792287372727 1103 114-115 0.8041096936089076 1103 116-117 0.8865434972215205 1103 118-119 0.8663347242567596 1103 120-121 0.8800714719762013 1103 122-123 0.7139074740711315 1103 124-125 0.9434139526545842 1104 1 -0.06247531384637739 1104 2 0.03956769203917787 1104 3 0.0723763113199638 1104 4 0.19133775617545012 1104 5 0.12566398214831054 1104 6 0.13517749502893395 1104 7 0.016588450811347855 1104 8 0.0938791054374093 1104 9 0.17745505847425846 1104 10-11 0.20856215315221505 1104 12-13 0.022758390107625814 1104 14-15 0.1758320511163589 1104 16-17 0.15538321631564145 1104 18-19 0.14788558967416066 1104 20-21 0.024079694796483864 1104 22-23 0.20385263228550343 1104 24-25 0.051442448894192694 1104 26-27 0.3691396390116708 1104 28-29 0.23006486317041208 1104 30-31 0.21149265608996615 1104 32-33 0.20877575786894909 1104 34-35 0.2735597152099132 1104 36-37 0.2702204215491548 1104 38-39 0.461870262321888 1104 40-41 0.46543078090174106 1104 42-43 0.3173678084439615 1104 44-45 0.209318827030458 1104 46-47 0.23405272928936682 1104 48-49 0.15598770311659393 1104 50-51 0.30967028559506105 1104 52-53 0.3486508664838297 1104 54-55 0.296104615267204 1104 56-57 0.35185619285439884 1104 58-59 0.3024442540588055 1104 60-61 0.2693766625508758 1104 62-63 0.16299765198389338 1104 64-65 0.21513421725415327 1104 66-67 0.3032658029832831 1104 68-69 0.38284086609341017 1104 70-71 0.406508272009134 1104 72-73 0.422806926989054 1104 74-75 0.3993641806048629 1104 76-77 0.3551615839316007 1104 78-79 0.5464373621718757 1104 80-81 0.5459610099642624 1104 82-83 0.6040911066392418 1104 84-85 0.525160732858236 1104 86-87 0.5350347085349902 1104 88-89 0.7274441186356135 1104 90-91 0.5023745315615038 1104 92-93 0.39357405156878755 1104 94-95 0.5366372056904147 1104 96-97 0.6084156420371407 1104 98-99 0.5379732098579098 1104 100-101 0.4572569912561235 1104 102-103 0.43516875709140024 1104 104-105 0.5062461798941094 1104 106-107 0.549125940855621 1104 108-109 0.38096126659575447 1104 110-111 0.3697132070621869 1104 112-113 0.2902744963754209 1104 114-115 0.050042820141875666 1104 116-117 0.2141459355421098 1104 118-119 0.12237642098161672 1104 120-121 0.3067072388208132 1104 122-123 0.48655211409421995 1104 124-125 0.2785784310848278 1105 1 0.1112290683059669 1105 2 0.13261627221640993 1105 3 0.2153772373648053 1105 4 0.24014437503026187 1105 5 0.17047914494969518 1105 6 0.20622338494450787 1105 7 0.21616192942781964 1105 8 0.1824461693345043 1105 9 0.20922042439715938 1105 10-11 0.12431370275645293 1105 12-13 0.24115592696353616 1105 14-15 0.19513467851511734 1105 16-17 0.2816710607941886 1105 18-19 0.23464551423867874 1105 20-21 0.2519969050357105 1105 22-23 0.3832082537832662 1105 24-25 0.4435643082840883 1105 26-27 0.3659430944432245 1105 28-29 0.45653724067225454 1105 30-31 0.439495373389434 1105 32-33 0.4989735394170012 1105 34-35 0.43965329305513734 1105 36-37 0.3871099247970733 1105 38-39 0.37136560168014654 1105 40-41 0.4141248548906944 1105 42-43 0.42625682501137163 1105 44-45 0.4591488268276791 1105 46-47 0.42906729590713866 1105 48-49 0.54620339721086 1105 50-51 0.4619712028526166 1105 52-53 0.49622016775933986 1105 54-55 0.4754524633958468 1105 56-57 0.47876827267000266 1105 58-59 0.44747436741480584 1105 60-61 0.48068556272053087 1105 62-63 0.480520853800833 1105 64-65 0.6997926283775087 1105 66-67 0.6947681586521028 1105 68-69 0.5108648756647938 1105 70-71 0.4800335286992983 1105 72-73 0.5469212698689887 1105 74-75 0.5747415269794303 1105 76-77 0.6770818088861397 1105 78-79 0.6354944862956842 1105 80-81 0.5655313116385763 1105 82-83 0.5800403026024981 1105 84-85 0.570430468766471 1105 86-87 0.6895608371943887 1105 88-89 0.6299900660065241 1105 90-91 0.5912396872654639 1105 92-93 0.6693177602093954 1105 94-95 0.7540093337685008 1105 96-97 0.7476268816529306 1105 98-99 0.7969920078922605 1105 100-101 0.8355409017339497 1105 102-103 0.6942203384650547 1105 104-105 0.7875333146302523 1105 106-107 0.8171263599416108 1105 108-109 0.847572280445867 1105 110-111 0.8974445323878228 1105 112-113 0.9864990047943145 1105 114-115 0.8788945245375501 1105 116-117 0.9615567276822183 1105 118-119 0.8579605389746021 1105 120-121 0.9990933927605568 1105 122-123 0.8966493825999073 1105 124-125 0.6745166767368111 1106 1 0.021744316363296434 1106 2 0.11824881550186461 1106 3 0.17507009727968637 1106 4 0.17176631758621852 1106 5 0.10220229855713114 1106 6 0.21725732352422256 1106 7 0.11722458100265243 1106 8 0.1482503717205077 1106 9 0.1799955721201698 1106 10-11 0.13345170692608121 1106 12-13 0.10995965101450622 1106 14-15 0.11869487072586793 1106 16-17 0.1821834981496977 1106 18-19 0.25189103935642265 1106 20-21 0.2172415456848995 1106 22-23 0.2751596544281796 1106 24-25 0.3146510829006104 1106 26-27 0.2797815461333215 1106 28-29 0.5203843307578211 1106 30-31 0.41080977617330916 1106 32-33 0.4902000884295816 1106 34-35 0.522751111924741 1106 36-37 0.3127626716134344 1106 38-39 0.44314132056869937 1106 40-41 0.45468502260450094 1106 42-43 0.32323590075463215 1106 44-45 0.39458197789516447 1106 46-47 0.41274620803660156 1106 48-49 0.3996549519286461 1106 50-51 0.3899949958302429 1106 52-53 0.4337223434746136 1106 54-55 0.5523341831327784 1106 56-57 0.5300294139438648 1106 58-59 0.7027531361726957 1106 60-61 0.49027579333991156 1106 62-63 0.42620661991691833 1106 64-65 0.41758624034851977 1106 66-67 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-0.7255843179332544 2216 22-23 -0.8905818273015811 2216 24-25 -0.6392598045672742 2216 26-27 -0.783424624993053 2216 28-29 -1.1614020904496059 2216 30-31 -0.942686292905222 2216 32-33 -0.9365696450581993 2216 34-35 -1.1500306550629418 2216 36-37 -1.1055573002625465 2216 38-39 -1.4471736335277825 2216 40-41 -1.121483745539372 2216 42-43 -1.2880655121062148 2216 44-45 -1.0418136275082333 2216 46-47 -1.0107809655975686 2216 48-49 -1.2937667457097461 2216 50-51 -1.2678603510636108 2216 52-53 -1.1212622461777997 2216 54-55 -1.3088135629605873 2216 56-57 -1.0725738594039314 2216 58-59 -1.0213536040938962 2216 60-61 -1.0719952759296874 2216 62-63 -1.0136872926955505 2216 64-65 -1.1523474236458568 2216 66-67 -1.190156115750149 2216 68-69 -1.097811246848682 2216 70-71 -1.2707659382490668 2216 72-73 -0.9720488045416609 2216 74-75 -1.0243818771254212 2216 76-77 -1.367980612520789 2216 78-79 -1.1050041777602395 2216 80-81 -1.2247276780827185 2216 82-83 -1.1882115223037424 2216 84-85 -1.2809311112940414 2216 86-87 -1.4305509224284982 2216 88-89 -1.4466571011069291 2216 90-91 -1.4829112865703635 2216 92-93 -1.5632630318098606 2216 94-95 -1.7293493777139872 2216 96-97 -1.8470019380531824 2216 98-99 -1.945529357267997 2216 100-101 -1.849359725684618 2216 102-103 -1.8459535663149502 2216 104-105 -1.835793440274287 2216 106-107 -1.927499476266565 2216 108-109 -1.8717126082475595 2216 110-111 -1.6735378561343275 2216 112-113 -1.4334040204725973 2216 114-115 -1.6171760981737222 2216 116-117 -1.6353649654418128 2216 118-119 -1.5303549780876935 2216 120-121 -1.8451956396800107 2216 122-123 -1.747843080200294 2216 124-125 -2.055667371936199 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 14.0 4 63.0 5 187.0 6 467.0 7 1297.0 8 1660.0 9 1880.0 10 2576.0 11 3984.0 12 5352.0 13 5714.0 14 7115.0 15 8969.0 16 5092.0 17 3715.0 18 4037.0 19 5494.0 20 6549.0 21 7795.0 22 5273.0 23 4316.0 24 5580.0 25 7157.0 26 9208.0 27 11495.0 28 14255.0 29 17725.0 30 22715.0 31 30276.0 32 40564.0 33 54489.0 34 78336.0 35 119403.0 36 249869.0 37 117244.0 38 6239.0 39 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.332753403233426 18.73825872902766 11.18555603883064 17.74343182890828 2 17.367715536302097 21.306374385612862 35.577243920208474 25.748666157876563 3 31.161789699253678 21.419285265646938 25.79908833961428 21.619836695485112 4 16.671463860943785 20.661111168908633 27.448736488384434 35.21868848176315 5 16.8185765893951 32.44193083440356 27.394371402437333 23.345121173764007 6 34.07309137345369 33.68294896396927 9.335591748701642 22.9083679138754 7 28.522754562920273 37.42830026601641 12.30129590630311 21.747649264760216 8 37.49076331929358 26.22974857755117 13.443411475652109 22.83607662750314 9 34.84295333628907 14.678240227591813 17.030591886499668 33.44821454961945 10-11 28.5034152626912 26.170460633266828 17.639520062070492 27.68660404197148 12-13 32.84286558782236 25.535554293209195 19.12437421488214 22.497205904086307 14-15 36.29894286189315 19.347670970959875 21.989303923741964 22.364082243405008 16-17 30.662085273036283 21.992248115717135 24.70165521318259 22.64401139806399 18-19 30.74631225522796 22.51083000812828 24.020623198847264 22.7222345377965 20-21 34.80669936451637 21.053628168181483 20.80799019064509 23.33168227665706 22-23 30.66318212887017 24.307364405527228 19.92790770708638 25.10154575851622 24-25 27.7618252604744 25.70851113943693 19.2119494938299 27.317714106258773 26-27 26.801210467006577 22.955922559668956 22.383132897362003 27.85973407596246 28-29 27.624545093475213 19.87987696741299 27.057528356609765 25.438049582502032 30-31 29.90404243331117 22.602330876376264 23.529174056011232 23.964452634301338 32-33 26.000852083795166 20.324680872681594 26.806117453631863 26.868349589891377 34-35 22.796647546737606 21.731715990541637 26.551935546442028 28.919700916278725 36-37 26.539523756742778 24.99665170324392 21.163429210079066 27.300395329934236 38-39 28.082107163969557 19.772211907928767 24.007287741077366 28.138393187024313 40-41 24.882982801300525 20.923448699475355 25.425406875785118 28.768161623439003 42-43 26.783949419936455 20.310999039385205 26.402647694524497 26.502403846153843 44-45 27.59464133968817 18.487678267937635 27.868335734870314 26.04934465750388 46-47 24.91825537574817 19.488645810241632 28.001286207788368 27.591812606221826 48-49 24.395690164782383 20.439157984186803 27.988412584053794 27.17673926697702 50-51 24.533836270597796 21.170068074336807 26.3660473472253 27.930048307840096 52-53 22.9065063178896 22.305429320919234 25.611699179782754 29.17636518140841 54-55 21.41639880292618 22.681189037907338 24.04625498780758 31.856157171358902 56-57 22.04293440109362 21.237264926476023 24.87992315081652 31.839877521613836 58-59 22.416384947905122 20.40896558412769 26.80808024828198 30.366569219685214 60-61 23.65692889603192 22.275063733096875 23.924965824281387 30.143041546589817 62-63 21.193332963866105 22.38734713293431 24.818903328899726 31.60041657429986 64-65 19.7082478940368 22.73620501736496 24.08112345747432 33.47442363112392 66-67 21.683396604596172 24.402675404566615 21.508015129683 32.40591286115422 68-69 22.684537334663414 22.585993497376784 22.38682756964457 32.34264159831523 70-71 21.80820725264169 22.613414893223972 22.732337157319147 32.846040696815194 72-73 22.993100199512302 21.78707834552575 22.827474968595286 32.392346486366655 74-75 24.046890009606148 20.220190922190202 23.79109168329269 31.941827384910958 76-77 23.014113648119412 20.77300626246952 24.252290696815194 31.960589392595878 78-79 22.901195226483413 20.847881105445946 24.82311756447203 31.42780610359861 80-81 22.970181685509495 21.481575131160866 24.308807636887607 31.239435546442028 82-83 21.80572489470184 22.28839919086677 23.976691236237347 31.929184678194044 84-85 21.50564823025198 21.270805623291213 23.90891709155398 33.31462905490283 86-87 21.86039449863297 20.218112669031257 24.73987197960541 33.18162085273036 88-89 22.22160644350846 19.86636832187985 25.70747201285746 32.20455322175423 90-91 22.42920084238528 19.786990597059038 25.34747238232469 32.43633617823099 92-93 21.683223416832927 19.288498485184363 25.779171746841058 33.249106351141656 94-95 20.67607884430651 19.638164579176827 25.737779871425403 33.94797670509126 96-97 21.31392937633932 20.141044114387054 24.959762709672653 33.58526379960097 98-99 21.90952209414025 19.636317243035542 25.40537482450307 33.048785838321145 100-101 21.667694247395257 20.5448025197665 25.227222345377964 32.56028088746028 102-103 21.954666371092884 21.05784240375379 24.978986551392893 32.008504673760434 104-105 21.97856628242075 21.822985941771964 25.092597724081873 31.105850051725415 106-107 21.393447260224153 23.930593191865707 24.37811088878908 30.29784865912106 108-109 20.877438483706495 25.03498392817557 24.234914191236236 29.852663396881695 110-111 20.72367585491845 26.341117599924257 23.86110937922148 29.074097165935807 112-113 20.507198561846323 27.804997168375166 23.13371099876979 28.554093271008725 114-115 19.88301696611914 28.330226524736794 22.81336576191089 28.973390747233168 116-117 19.71219433060011 28.266982884187836 23.08397526529307 28.93684751991899 118-119 20.041508028830474 28.665710269340344 23.403716094319634 27.889065607509544 120-121 20.291799468542337 29.23253841753083 23.016342640673773 27.459319473253064 122-123 19.868698158171842 29.180483456799244 23.170987973619553 27.779830411409357 124-125 19.13660057988824 30.629440328738077 22.793326607815498 27.440632483558186 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.5 2 1.5 3 2.5 4 2.0 5 2.5 6 5.0 7 8.0 8 11.0 9 13.5 10 17.5 11 16.5 12 18.5 13 28.5 14 28.0 15 24.0 16 25.5 17 29.0 18 38.0 19 43.0 20 52.5 21 86.0 22 125.0 23 178.5 24 251.0 25 335.5 26 411.0 27 546.5 28 760.0 29 930.5 30 1140.5 31 1408.5 32 1658.5 33 1885.5 34 2052.5 35 2178.5 36 2216.5 37 2215.5 38 2206.5 39 2142.5 40 2105.5 41 2112.5 42 2218.0 43 2456.0 44 2832.5 45 3536.5 46 4662.0 47 6032.5 48 7891.5 49 11689.5 50 18884.0 51 30253.0 52 47606.5 53 71859.0 54 96788.5 55 112488.5 56 113377.5 57 98875.5 58 75118.0 59 49784.5 60 28797.5 61 16358.5 62 10225.0 63 6765.5 64 4918.0 65 3896.0 66 3072.5 67 2243.0 68 1696.0 69 1359.5 70 980.0 71 722.0 72 505.0 73 322.5 74 227.5 75 146.5 76 78.0 77 41.0 78 21.5 79 14.5 80 10.0 81 8.5 82 5.5 83 5.5 84 6.5 85 4.0 86 3.0 87 2.0 88 2.0 89 2.5 90 2.5 91 1.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18935195448163747 2 5.772925441513338E-4 3 0.0 4 0.11811405453336289 5 0.028633710189906156 6 0.0020782531589448017 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 1.1545850883026676E-4 108-109 0.0 110-111 6.927510529816005E-4 112-113 1.7318776324540012E-4 114-115 4.0410478090593363E-4 116-117 0.00479152811645607 118-119 0.0016741483780388679 120-121 4.0410478090593363E-4 122-123 0.0018473361412842681 124-125 0.02949964900613316 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 866112.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.37559008294089 #Duplication Level Percentage of deduplicated Percentage of total 1 79.51837318965408 46.419319573815635 2 10.730895004814744 12.52844656048287 3 3.624664028206885 6.347757044969593 4 1.8496461695749946 4.3189674637436655 5 1.0712151626831499 3.1266408613711194 6 0.7034635667315402 2.463906048588236 7 0.47573224405885645 1.9439805327892172 8 0.34040544681723933 1.58970950603228 9 0.27930972798021764 1.4674383168075817 >10 1.3405225162502623 13.448747194507337 >50 0.04523265264469403 1.7648252013222172 >100 0.017775251515409443 2.033891234204615 >500 0.0019750279056486506 0.864585352249439 >1k 5.925083716945951E-4 0.8461384051418365 >5k 1.9750279056486502E-4 0.8356467039743878 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT 7248 0.8368432720017736 No Hit ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT 3991 0.4607949087415946 TruSeq Adapter, Index 10 (95% over 21bp) ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT 2323 0.26821011601270967 RNA PCR Primer, Index 10 (95% over 23bp) CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCT 1025 0.11834497155102343 TruSeq Adapter, Index 10 (96% over 26bp) ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA 980 0.11314933865366142 No Hit GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT 900 0.10391265794724008 No Hit GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT 874 0.10091073671765313 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 1.1545850883026676E-4 0.0 0.0 5.772925441513338E-5 0.0 12-13 2.3091701766053352E-4 0.0 0.0 1.1545850883026676E-4 0.0 14-15 2.886462720756669E-4 0.0 0.0 1.1545850883026676E-4 0.0 16-17 3.463755264908003E-4 0.0 0.0 3.463755264908003E-4 0.0 18-19 4.0410478090593363E-4 0.0 0.0 8.659388162270007E-4 0.0 20-21 4.6183403532106703E-4 0.0 0.0 0.003232838247247469 0.0 22-23 4.6183403532106703E-4 0.0 0.0 0.011545850883026676 0.0 24-25 4.6183403532106703E-4 0.0 0.0 0.025169954924998152 0.0 26-27 5.195632897362004E-4 0.0 0.0 0.06656183034064878 0.0 28-29 8.082095618118673E-4 0.0 0.0 0.2469657503879406 0.0 30-31 0.0010391265794724009 0.0 0.0 0.656612539717727 0.0 32-33 0.0010391265794724009 0.0 0.0 1.2765092736274293 0.0 34-35 0.0010391265794724009 0.0 0.0 2.185340925884874 0.0 36-37 0.0010391265794724009 0.0 0.0 3.42380662085273 0.0 38-39 0.0010391265794724009 0.0 0.0 5.1833365661715805 0.0 40-41 0.0012700435971329345 0.0 5.772925441513338E-5 7.420171986994754 0.0 42-43 0.0016741483780388679 0.0 1.1545850883026676E-4 9.198059835217617 0.0 44-45 0.0017318776324540014 0.0 1.1545850883026676E-4 10.731117915465898 0.0 46-47 0.0017896068868691348 0.0 1.1545850883026676E-4 12.550108992832335 0.0 48-49 0.0018473361412842681 0.0 1.1545850883026676E-4 14.420479106628243 0.0 50-51 0.0018473361412842681 0.0 1.1545850883026676E-4 16.337148082465085 0.0 52-53 0.0018473361412842681 0.0 2.3091701766053352E-4 18.23338090223897 0.0 54-55 0.0018473361412842681 0.0 2.3091701766053352E-4 19.938645348407597 0.0 56-57 0.0018473361412842681 0.0 2.3091701766053352E-4 21.6384832446612 0.0 58-59 0.0018473361412842681 0.0 2.3091701766053352E-4 23.39847502401537 0.0 60-61 0.0018473361412842681 0.0 2.3091701766053352E-4 25.233168458582725 0.0 62-63 0.0018473361412842681 0.0 3.463755264908003E-4 27.023063991723937 0.0 64-65 0.0018473361412842681 0.0 3.463755264908003E-4 28.743742148821397 0.0 66-67 0.0018473361412842681 0.0 3.463755264908003E-4 30.5050039717727 0.0 68-69 0.0018473361412842681 0.0 3.463755264908003E-4 32.43454657134412 0.0 70-71 0.0018473361412842681 0.0 3.463755264908003E-4 34.6515808579029 0.0 72-73 0.0018473361412842681 0.0 4.0410478090593363E-4 36.77301549915022 0.0 74-75 0.0018473361412842681 0.0 4.6183403532106703E-4 38.64159600605926 0.0 76-77 0.0018473361412842681 0.0 4.6183403532106703E-4 40.526109787186876 0.0 78-79 0.0018473361412842681 0.0 5.195632897362004E-4 42.38250942141432 0.0 80-81 0.0018473361412842681 0.0 5.772925441513338E-4 44.31401481563585 0.0 82-83 0.0018473361412842681 0.0 5.772925441513338E-4 46.436315395699395 0.0 84-85 0.0018473361412842681 0.0 8.082095618118673E-4 48.53841073671765 0.0 86-87 0.0018473361412842681 0.0 9.236680706421341E-4 50.33078862779871 0.0 88-89 0.0018473361412842681 0.0 0.0011545850883026676 52.02629682997119 0.0 90-91 0.0018473361412842681 0.0 0.0013855021059632011 53.85192677159536 0.0 92-93 0.0018473361412842681 0.0 0.0013855021059632011 55.59436885760733 0.0 94-95 0.0018473361412842681 0.0 0.0015009606147934678 57.25645182147343 0.0 96-97 0.0018473361412842681 0.0 0.0018473361412842681 58.84712369762802 0.0 98-99 0.0018473361412842681 0.0 0.0018473361412842681 60.37238832853026 0.0 100-101 0.0018473361412842681 0.0 0.0018473361412842681 62.00797356461982 0.0 102-103 0.0018473361412842681 0.0 0.0019050653956994017 63.578844306510014 0.0 104-105 0.0018473361412842681 0.0 0.0021937116677750684 65.03200509864774 0.0 106-107 0.0018473361412842681 0.0 0.0021937116677750684 66.42125960614794 0.0 108-109 0.0018473361412842681 0.0 0.002309170176605335 67.68581892411143 0.0 110-111 0.0018473361412842681 0.0 0.002309170176605335 68.98720950639178 0.0 112-113 0.0018473361412842681 0.0 0.0025400871942658685 70.35054357865957 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTATACA 40 1.7422375E-4 59.70238 1 ATTCTTA 15 0.0042381594 59.495583 28-29 TATAACA 35 0.007749953 51.07583 3 TATGATA 60 2.1737496E-5 49.65705 4 TAGGATT 25 5.33531E-4 47.596466 14-15 CTTTACA 130 1.8189894E-11 45.924908 1 ATGTACA 85 3.4445202E-6 42.062447 4 TACAGGT 85 3.4816676E-6 41.996883 7 GTCTTAG 75 8.024779E-5 39.801586 1 CAAGGTA 60 0.0012988113 39.725643 4 CAGATGT 90 5.148464E-6 39.672882 5 TAGGGAG 195 0.0 39.672882 5 AATTGAG 30 0.0013091734 39.66372 66-67 TGGATCG 30 0.0013091734 39.66372 38-39 GCTCGGA 65 0.0019245463 36.669823 3 TATACGG 285 0.0 35.543995 2 GTCAGAT 105 1.4682513E-5 34.050552 3 GATGTGT 140 8.050665E-8 34.00533 5 ACGGGCG 70 0.0027864666 34.00533 5 TTTTAGC 35 0.00279039 33.997475 80-81 >>END_MODULE