FastQCFastQC Report
Thu 26 May 2016
SRR935992_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935992_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5480431
Sequences flagged as poor quality0
Sequence length125
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT224280.40923788658227794No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT189970.34663332135738956No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC153480.28005096679439995No Hit
GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT151440.27632863181746103No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT142810.26058169512580304No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG112540.20534881289446033No Hit
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA111730.2038708269477346No Hit
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC94380.17221273290367126No Hit
TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT83100.15163041009000933No Hit
GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT80950.14770736097215711No Hit
GGTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTC77060.14060937907985704No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA75440.1376534071864056No Hit
AATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTTGGGTT68800.12553757177127128No Hit
ATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAA59740.10900602525604282No Hit
GGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCT59510.10858635023413304No Hit
AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG57610.10511946961835666No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT54970.10030233023643577No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA158450.071.1421361
GTATCAA283650.054.2759861
GTGGTAT58750.048.3736081
TGGTATC58700.047.7373352
GAGTAAG29800.047.5837481
GAGTTAG31800.044.591061
GATTGTG152750.044.3947539
GAGTAGT55250.042.2719041
AGTAGTA44250.041.9485242
TATCAAC367500.041.7836152
AGGGTAA109150.039.2933966
GAGTACG23150.039.1192551
GGGATTG183900.037.812977
CGGGATT45400.037.0799036
GGGGTAA170800.036.9517979
ATCAACG417900.036.6864363
TCAACGC423750.036.123814
TAGTAGG75750.035.735852
GAGTAGG54450.035.561781
GAGTCAG149550.035.4171261
Produced by FastQC (version 0.11.5)