FastQCFastQC Report
Thu 26 May 2016
SRR935992_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935992_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5480431
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT227350.4148396357877692No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT209960.38310855478337374No Hit
GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT160020.29198433480870395No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT151600.2766205796587896No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC129760.23676969931744418No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG123530.22540198024571423No Hit
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA113930.207885109766002No Hit
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC105410.19233888721525733No Hit
TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT89030.16245072695924828No Hit
GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT77650.14168593674475602No Hit
GGTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTC75360.13750743326574133No Hit
AATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTTGGGTT73090.13336542326689269No Hit
ATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAA63960.11670614957108301No Hit
AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG63010.1149727092631948No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT62950.1148632288226966No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA61830.11281959393339686No Hit
GGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCT58550.1068346631861618No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA168700.073.7598951
GTATCAA302650.054.0367241
TGGTATC60750.052.0820582
GTGGTAT64700.050.20331
GATTGTG151300.046.2827539
AGGGTAA109450.044.4115456
TATCAAC394350.041.325242
GATAGCG33750.038.7820329
GGGATTG185350.038.7111787
CGGGATT43350.038.428956
TAGTAGG72000.038.3167272
TCGTAGG21500.037.691232
ATCAACG445500.036.553073
GTAGTAG76600.036.4020421
GAGTAGT50700.036.390511
TCAACGC452400.036.0219044
GAGTAAG30050.035.964311
GTACGGG21950.035.9068261
CAACGCA470700.034.7090155
AGTAGTA41400.034.686192
Produced by FastQC (version 0.11.5)