FastQCFastQC Report
Thu 26 May 2016
SRR935991_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935991_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448261
Sequences flagged as poor quality0
Sequence length125
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT14420.32168758825773375No Hit
ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA14270.31834132347003197No Hit
GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT13250.29558672291365967No Hit
TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC8600.19185251449490365No Hit
GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA8150.1818137201317982No Hit
ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC8090.1804752142167175No Hit
GGGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGA4500.10038794363105422No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACAAC2150.063.7492331
TACGGGC655.474776E-754.921794
GTGTACG1250.052.4406741
GTCTAAG602.1713602E-549.6597251
GTATGAG909.58953E-846.349081
GTTCTAG653.484889E-545.8397521
TGGAATC3550.043.576442
CTATAGA558.4584655E-443.33941
GTGGAAT4050.042.6705861
ATGGGAC705.4473836E-542.4990045
TATAGAG705.4473836E-542.4990042
ATATTAT300.001292794839.763325118-119
TATAGAA600.001307809639.6657372
GCGTTAT650.001923046136.6718031
TATACGG650.001937862336.614532
TGTAAGG1209.2733353E-734.707522
AGTCGTC1555.1113602E-934.543727
GTATACG700.002766218534.0523871
CGTTATA700.002787500633.9992032
TGTACGG1754.4383341E-1033.9992032