##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935991_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 448261 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.46176223227093 31.0 28.0 34.0 7.0 34.0 2 27.28222397219477 31.0 27.0 34.0 7.0 34.0 3 27.29626936093035 31.0 27.0 34.0 7.0 34.0 4 30.20699324723766 35.0 31.0 37.0 7.0 37.0 5 30.47646571974809 37.0 32.0 37.0 7.0 37.0 6 30.65509602664519 37.0 32.0 37.0 7.0 37.0 7 30.609388280488375 37.0 32.0 37.0 7.0 37.0 8 30.341481859898586 37.0 31.0 37.0 2.0 37.0 9 31.277463352823467 39.0 31.0 39.0 2.0 39.0 10-11 31.424927664909504 39.0 31.0 39.0 2.0 39.0 12-13 31.44014982342876 39.0 31.0 39.0 2.0 39.0 14-15 32.38946283526785 39.0 31.0 41.0 2.0 41.0 16-17 32.56607869076275 39.0 31.0 41.0 2.0 41.0 18-19 32.82379908133878 39.0 31.0 41.0 2.0 41.0 20-21 33.05080522285009 39.5 31.0 41.0 2.0 41.0 22-23 32.81698720165261 39.0 31.0 41.0 2.0 41.0 24-25 32.53063170786662 39.0 30.0 41.0 2.0 41.0 26-27 32.303498408293386 39.0 30.0 41.0 2.0 41.0 28-29 32.001680940345025 39.0 30.0 41.0 2.0 41.0 30-31 31.6790117810829 39.0 30.0 41.0 2.0 41.0 32-33 31.285226687130937 38.0 30.0 41.0 2.0 41.0 34-35 30.839871191114106 38.0 29.5 41.0 2.0 41.0 36-37 30.55211918949005 38.0 29.0 41.0 2.0 41.0 38-39 30.348935107002394 38.0 28.0 41.0 2.0 41.0 40-41 30.04927932610689 38.0 28.0 41.0 2.0 41.0 42-43 29.73447388909586 37.0 27.0 41.0 2.0 41.0 44-45 29.351430305112423 37.0 26.0 40.0 2.0 41.0 46-47 28.947773730036744 36.0 24.5 40.0 2.0 41.0 48-49 28.535102317622993 35.5 23.0 39.5 2.0 41.0 50-51 28.13458007723179 35.0 21.5 39.0 2.0 41.0 52-53 27.77634346954118 35.0 20.0 38.5 2.0 41.0 54-55 27.415481159413822 35.0 17.5 37.0 2.0 40.5 56-57 27.06833630407285 35.0 14.0 37.0 2.0 40.0 58-59 26.726750709965845 35.0 8.5 36.0 2.0 39.0 60-61 26.434407633053066 35.0 6.0 35.5 2.0 39.0 62-63 26.16653467511115 35.0 2.0 35.0 2.0 37.5 64-65 25.969480949714566 35.0 2.0 35.0 2.0 37.0 66-67 25.8535864150573 34.5 2.0 35.0 2.0 36.5 68-69 25.77061801048942 35.0 2.0 35.0 2.0 36.0 70-71 25.612512576378492 35.0 2.0 35.0 2.0 35.5 72-73 25.474112180180743 34.5 2.0 35.0 2.0 35.0 74-75 25.33466774936923 34.0 2.0 35.0 2.0 35.0 76-77 25.180631373240143 34.0 2.0 35.0 2.0 35.0 78-79 25.016483700344217 34.0 2.0 35.0 2.0 35.0 80-81 24.83402638195159 34.0 2.0 35.0 2.0 35.0 82-83 24.686594417091825 34.0 2.0 35.0 2.0 35.0 84-85 24.57133232647944 34.0 2.0 35.0 2.0 35.0 86-87 24.442170967360532 34.0 2.0 35.0 2.0 35.0 88-89 24.21536002462851 34.0 2.0 35.0 2.0 35.0 90-91 23.961426490370563 33.0 2.0 35.0 2.0 35.0 92-93 23.700236023209694 33.0 2.0 35.0 2.0 35.0 94-95 23.486462128090555 33.0 2.0 35.0 2.0 35.0 96-97 23.283116309471488 33.0 2.0 35.0 2.0 35.0 98-99 23.027163862124965 33.0 2.0 35.0 2.0 35.0 100-101 22.810506602180425 32.0 2.0 35.0 2.0 35.0 102-103 22.194559642708157 30.5 2.0 34.5 2.0 35.0 104-105 22.58822315570616 32.0 2.0 35.0 2.0 35.0 106-107 22.574704245963844 32.0 2.0 35.0 2.0 35.0 108-109 22.372734857594125 32.0 2.0 35.0 2.0 35.0 110-111 22.046741741976213 32.0 2.0 35.0 2.0 35.0 112-113 21.600781687454408 31.0 2.0 35.0 2.0 35.0 114-115 21.080524515851256 31.0 2.0 35.0 2.0 35.0 116-117 20.50331391756142 30.5 2.0 35.0 2.0 35.0 118-119 19.75970807185992 29.0 2.0 35.0 2.0 35.0 120-121 19.028960806315965 28.0 2.0 35.0 2.0 35.0 122-123 18.288010779434302 24.5 2.0 34.5 2.0 35.0 124-125 16.043966573045616 10.5 2.0 31.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.8023930775835382 1101 2 0.8280853788862039 1101 3 1.0958970324753246 1101 4 0.8951737987205846 1101 5 0.7231777337292691 1101 6 0.8026947779729419 1101 7 0.5971625384596173 1101 8 0.6495841316731585 1101 9 0.5391404769086776 1101 10-11 0.5900502740050477 1101 12-13 0.5639905770937439 1101 14-15 0.47017932138917473 1101 16-17 0.36086086577399357 1101 18-19 0.6785212389916211 1101 20-21 0.6141389835753444 1101 22-23 0.6098624190669142 1101 24-25 0.8027569234264789 1101 26-27 0.8679483125241276 1101 28-29 0.8706199497699103 1101 30-31 0.770051455227847 1101 32-33 0.9901484616621516 1101 34-35 0.8696079365405929 1101 36-37 0.5705961348311277 1101 38-39 0.750414055837954 1101 40-41 0.5526055684207094 1101 42-43 0.5031677073048897 1101 44-45 0.626482330103002 1101 46-47 0.5884148004249425 1101 48-49 0.5266017234184055 1101 50-51 0.480527640098412 1101 52-53 0.5113661069692199 1101 54-55 0.5448397292728195 1101 56-57 0.3238750486876256 1101 58-59 0.4667251760294384 1101 60-61 0.5943969287258177 1101 62-63 0.44717696150951625 1101 64-65 0.41199889206404094 1101 66-67 0.31455321609062636 1101 68-69 0.411327937626929 1101 70-71 0.22211818374982073 1101 72-73 0.407463832232942 1101 74-75 0.4605606250746419 1101 76-77 0.44604907529360105 1101 78-79 0.6503071097046416 1101 80-81 0.5573598942205606 1101 82-83 0.5613246945484676 1101 84-85 0.47286566493061244 1101 86-87 0.427751867952864 1101 88-89 0.18448127515863177 1101 90-91 0.10399851106214442 1101 92-93 0.12131394640107018 1101 94-95 0.39701936041268127 1101 96-97 0.2898610826570156 1101 98-99 0.47565988652355884 1101 100-101 0.2132562936267277 1101 102-103 0.4400364960512384 1101 104-105 0.5386113794476763 1101 106-107 0.4677994849606222 1101 108-109 0.48774678151683304 1101 110-111 0.5534501272846697 1101 112-113 0.6624475248575905 1101 114-115 0.41574587997017076 1101 116-117 0.3280456662664264 1101 118-119 0.18877135542276946 1101 120-121 -0.30373517615937473 1101 122-123 -0.2497690849962062 1101 124-125 -0.012861448125427444 1102 1 1.2454638875951538 1102 2 1.3229766028802885 1102 3 1.3630549953013862 1102 4 1.2934603126080084 1102 5 1.150734101877962 1102 6 1.059331566440573 1102 7 1.1799755334960516 1102 8 1.0050466937177234 1102 9 1.2879226872793588 1102 10-11 1.236138826122481 1102 12-13 1.282557413612917 1102 14-15 1.3434087410744624 1102 16-17 1.3378301207111534 1102 18-19 1.254398773578167 1102 20-21 1.29851035330816 1102 22-23 1.211775569874817 1102 24-25 1.1790011149989752 1102 26-27 1.3978138606284674 1102 28-29 1.5401435267051795 1102 30-31 1.204784201557974 1102 32-33 1.6133217772181752 1102 34-35 1.6066412821948326 1102 36-37 1.4221759611063405 1102 38-39 1.3796944708765047 1102 40-41 1.4273960029966943 1102 42-43 1.3258407060375959 1102 44-45 1.3629810364077883 1102 46-47 1.313920397636899 1102 48-49 1.4086196899407426 1102 50-51 1.5034544275099613 1102 52-53 1.5810870384297715 1102 54-55 1.3394326113089363 1102 56-57 1.5355245760928433 1102 58-59 1.533140914227772 1102 60-61 1.4265135725107996 1102 62-63 1.5124871183370594 1102 64-65 1.422657026502737 1102 66-67 1.5075827730660194 1102 68-69 1.5852490882236125 1102 70-71 1.5254616598296629 1102 72-73 1.4150213884690785 1102 74-75 1.58264321035292 1102 76-77 1.6644954529469977 1102 78-79 1.7096058740937004 1102 80-81 1.7601581701736748 1102 82-83 1.6427581880535875 1102 84-85 1.7018117653636047 1102 86-87 1.8057484885041362 1102 88-89 1.6227469315109353 1102 90-91 1.643408361627145 1102 92-93 1.6409767935866242 1102 94-95 1.7859810379921548 1102 96-97 1.6255254875572156 1102 98-99 1.7264783467622316 1102 100-101 1.6453999994034731 1102 102-103 1.5301166259487964 1102 104-105 1.5641882619062244 1102 106-107 1.2779464117744368 1102 108-109 1.4688314393476531 1102 110-111 1.528166966444033 1102 112-113 1.2503428939567556 1102 114-115 1.2766835449823155 1102 116-117 1.206971305193452 1102 118-119 1.2063533994612108 1102 120-121 1.3190685121920929 1102 122-123 0.9078241138632315 1102 124-125 0.6386626040448107 1103 1 0.9520851338932417 1103 2 0.8852623522396463 1103 3 0.937460003817705 1103 4 1.0850053723756865 1103 5 0.9483065673139848 1103 6 0.9011582018040123 1103 7 0.8676030864736965 1103 8 1.0609843327792419 1103 9 1.1805526186884627 1103 10-11 0.9852802890880064 1103 12-13 0.9800209315432191 1103 14-15 1.1966671921785093 1103 16-17 0.8663897119278374 1103 18-19 0.723598853370703 1103 20-21 0.8966490388795165 1103 22-23 1.108072889152382 1103 24-25 0.8618349777251169 1103 26-27 0.954409788140012 1103 28-29 1.187899361534626 1103 30-31 1.2701818599538406 1103 32-33 1.330044452109611 1103 34-35 1.4580537635893656 1103 36-37 1.6087984349819564 1103 38-39 1.5930014112929562 1103 40-41 1.568199145595166 1103 42-43 1.625045849587444 1103 44-45 1.8005679258797755 1103 46-47 1.675248636523488 1103 48-49 1.5783629727899502 1103 50-51 1.483365121244713 1103 52-53 1.5678706318560955 1103 54-55 1.4618834698459686 1103 56-57 1.5332689023829502 1103 58-59 1.6551191554160667 1103 60-61 1.713206632094348 1103 62-63 1.288201031393882 1103 64-65 1.5293427206211057 1103 66-67 1.5663238923098355 1103 68-69 1.5871119371241669 1103 70-71 1.7499886588629465 1103 72-73 1.5688828870343485 1103 74-75 1.5451760096900244 1103 76-77 1.4500806238104467 1103 78-79 1.4038516044256077 1103 80-81 1.298622777579034 1103 82-83 1.2367614718234812 1103 84-85 1.322496446730515 1103 86-87 1.4277267296924308 1103 88-89 1.6231627733989527 1103 90-91 1.3036701425352462 1103 92-93 1.305671663847022 1103 94-95 1.4061209818304796 1103 96-97 1.1384564823553553 1103 98-99 1.3385055376045045 1103 100-101 1.304165670197868 1103 102-103 1.213298813798847 1103 104-105 1.1857644714537088 1103 106-107 1.3051203748550435 1103 108-109 1.1260150696013014 1103 110-111 0.9060613640870869 1103 112-113 0.7822721854910739 1103 114-115 0.6694254928409578 1103 116-117 0.8071416315756288 1103 118-119 0.3959404730698246 1103 120-121 0.39639208628406664 1103 122-123 0.5264249823743334 1103 124-125 0.36226738545928683 1104 1 0.12669869999616878 1104 2 0.043727527726098 1104 3 0.07450310256293236 1104 4 0.15334507050262047 1104 5 0.050506967386724 1104 6 0.32396234801241164 1104 7 0.2034823372465624 1104 8 0.10073701508511235 1104 9 0.056930141874499185 1104 10-11 0.35511116742952 1104 12-13 0.35099747273252646 1104 14-15 0.18584704306049105 1104 16-17 0.20223259245157266 1104 18-19 -0.02541005734033064 1104 20-21 -0.09673157577768876 1104 22-23 -0.017822728232594898 1104 24-25 0.03384807101480902 1104 26-27 0.05591915347916654 1104 28-29 0.08577170196014805 1104 30-31 0.23628478775889405 1104 32-33 0.29138287727782597 1104 34-35 0.31935581850843775 1104 36-37 0.5092348779715934 1104 38-39 0.37959897448626734 1104 40-41 0.43499310813380987 1104 42-43 0.356287893595443 1104 44-45 0.05004964982958526 1104 46-47 0.4141284328500916 1104 48-49 0.3880556441847496 1104 50-51 0.484947227082138 1104 52-53 0.4208984550966832 1104 54-55 0.3953259139267118 1104 56-57 0.37751331045714664 1104 58-59 0.4605019522882259 1104 60-61 0.6230084142365513 1104 62-63 0.37721721615474735 1104 64-65 0.3956638699209769 1104 66-67 0.4631960508841182 1104 68-69 0.3754552722760103 1104 70-71 0.31250911712808715 1104 72-73 0.2541893063833207 1104 74-75 0.4662889393135927 1104 76-77 0.3997441990059336 1104 78-79 0.4234369835854537 1104 80-81 0.39460386504948275 1104 82-83 0.39659053165253866 1104 84-85 0.566286226690508 1104 86-87 0.6013582990686821 1104 88-89 0.6171434971669072 1104 90-91 0.5282382458267563 1104 92-93 0.7395687360419672 1104 94-95 0.651729274247284 1104 96-97 0.6333282772271502 1104 98-99 0.6870780053259082 1104 100-101 0.36561320611349046 1104 102-103 0.5287561696817349 1104 104-105 0.5420110617428513 1104 106-107 0.5608214114071401 1104 108-109 0.47680612252001353 1104 110-111 0.5544519805628099 1104 112-113 0.7287533638871437 1104 114-115 1.2086871767834921 1104 116-117 0.7239446866765533 1104 118-119 0.4453676416459338 1104 120-121 0.45629388661139814 1104 122-123 0.27663411130686555 1104 124-125 0.3312435629461028 1105 1 0.2465689304771992 1105 2 0.17008623415561175 1105 3 0.36972875821893325 1105 4 0.25349604522823554 1105 5 0.07053293186667986 1105 6 -0.005805055724675157 1105 7 0.05721136008843075 1105 8 -0.016943949672466374 1105 9 0.19732077245177138 1105 10-11 0.24487185530315614 1105 12-13 0.07686861805164469 1105 14-15 0.19164806841948234 1105 16-17 0.22831341020048512 1105 18-19 0.278221003792531 1105 20-21 0.2787486481196737 1105 22-23 0.2287790184207097 1105 24-25 0.4717765708654156 1105 26-27 0.50130649222573 1105 28-29 0.4357184245394663 1105 30-31 0.5461742932968328 1105 32-33 0.6494706012611289 1105 34-35 0.5811237827821607 1105 36-37 0.31363667697690545 1105 38-39 0.3909247229480961 1105 40-41 0.45087453877136596 1105 42-43 0.43160868183686674 1105 44-45 0.3500729173879975 1105 46-47 0.34838700416912616 1105 48-49 0.2464104162816554 1105 50-51 0.4249499768844949 1105 52-53 0.4385553590836331 1105 54-55 0.3775870446771741 1105 56-57 0.2068946842478283 1105 58-59 0.34171181244490967 1105 60-61 0.37893437051998546 1105 62-63 0.3869472860367438 1105 64-65 0.31081131485222 1105 66-67 0.4732843882848705 1105 68-69 0.5622607158270618 1105 70-71 0.49174335005220726 1105 72-73 0.3905900795008286 1105 74-75 0.2893166832329612 1105 76-77 0.369386645378178 1105 78-79 0.46746951586258234 1105 80-81 0.4266774011102328 1105 82-83 0.46854281200546666 1105 84-85 0.2869856283440875 1105 86-87 0.45276849819138576 1105 88-89 0.6772174245456881 1105 90-91 0.6347362403218639 1105 92-93 0.8034209976222044 1105 94-95 0.6904049800078589 1105 96-97 0.6129480112895358 1105 98-99 0.5075899444918193 1105 100-101 0.6193459306123792 1105 102-103 0.44818828261400157 1105 104-105 0.27925687027728685 1105 106-107 0.33717787040203717 1105 108-109 0.4744886208212158 1105 110-111 0.414863816346724 1105 112-113 0.5831420748908513 1105 114-115 0.6566022184667943 1105 116-117 0.6491117239495985 1105 118-119 0.6266351418905067 1105 120-121 0.6378733836619723 1105 122-123 0.5152931596108452 1105 124-125 0.27512379847724944 1106 1 0.4471836861346503 1106 2 0.501658654350237 1106 3 0.5865442529019553 1106 4 -0.10728487705741685 1106 5 0.4787443834660863 1106 6 0.3637432358176156 1106 7 0.4538581013001384 1106 8 0.7259415977365045 1106 9 0.5682030634817643 1106 10-11 0.534626181291042 1106 12-13 0.64616431197231 1106 14-15 0.7324250850792708 1106 16-17 0.8120726004678716 1106 18-19 0.702737175366174 1106 20-21 0.854402396632878 1106 22-23 0.8924615163981571 1106 24-25 0.7679574267047045 1106 26-27 1.111465430909945 1106 28-29 0.8349951229160908 1106 30-31 0.902957021300427 1106 32-33 1.1083106303757901 1106 34-35 1.0506928705141867 1106 36-37 1.311833495159874 1106 38-39 1.2707443033215675 1106 40-41 1.2836186467289536 1106 42-43 1.0961255635242964 1106 44-45 1.2391341215888296 1106 46-47 1.0879819349710722 1106 48-49 1.1481344711328774 1106 50-51 1.106494372945395 1106 52-53 1.1328627825625333 1106 54-55 0.9692094771719759 1106 56-57 1.0986796820794424 1106 58-59 0.9827137830106949 1106 60-61 1.056656832687402 1106 62-63 1.1324361613432963 1106 64-65 1.2014690070331042 1106 66-67 1.2264145952576762 1106 68-69 1.341987220570875 1106 70-71 1.1203183291926138 1106 72-73 1.177900853195169 1106 74-75 1.288661096032257 1106 76-77 1.3841291150017945 1106 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2216 84-85 -2.8765353694065645 2216 86-87 -2.86407897921854 2216 88-89 -2.6703421135269885 2216 90-91 -2.6600081382482976 2216 92-93 -2.770520418924008 2216 94-95 -2.817759523628972 2216 96-97 -2.8508914504136307 2216 98-99 -2.787533189750011 2216 100-101 -2.8774153219094387 2216 102-103 -2.890291747686451 2216 104-105 -3.229383976589478 2216 106-107 -3.0037650379777396 2216 108-109 -3.264412485299939 2216 110-111 -3.4976774409675073 2216 112-113 -3.6010182083256375 2216 114-115 -3.3931568078992385 2216 116-117 -3.2519615459486033 2216 118-119 -3.1845619192746675 2216 120-121 -2.4048776965646645 2216 122-123 -2.1739880548808124 2216 124-125 -1.972745700869588 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 44606.0 3 4697.0 4 2381.0 5 4936.0 6 13618.0 7 11262.0 8 7260.0 9 7281.0 10 5216.0 11 3373.0 12 2524.0 13 1969.0 14 2067.0 15 2189.0 16 1850.0 17 1838.0 18 1738.0 19 2002.0 20 2397.0 21 2700.0 22 3255.0 23 3566.0 24 4110.0 25 4368.0 26 4786.0 27 5370.0 28 6427.0 29 7749.0 30 9992.0 31 13334.0 32 17509.0 33 24085.0 34 35240.0 35 55404.0 36 92908.0 37 30955.0 38 3287.0 39 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 55.693719616814406 18.90727766657135 10.947553259937088 14.451449456677151 2 20.551551797228854 25.02086168812334 33.50565608335751 20.9219304312903 3 30.064341680201323 25.4296881971836 24.165833764657588 20.340136357957487 4 16.317715305667114 24.444890673806334 26.923025435073626 32.314368585452925 5 15.829594711867735 36.41926696992074 24.85181227398165 22.89932604422988 6 30.510126912669183 37.362161776286584 9.055661768478632 23.072049542565605 7 28.037014150238367 42.3545657552185 11.825699759738189 17.782720334804946 8 34.70426695281076 30.227390861087006 9.873979105829441 25.19436308027279 9 35.1714737619378 19.367065169622162 14.06970492637102 31.391756142069017 10-11 30.296189050575446 29.069002210765603 13.859894124182118 26.774914614476835 12-13 32.51030091843814 30.863604016410086 15.93926306326002 20.686832001891755 14-15 35.248884020693296 25.035860804308204 19.460425957199043 20.254829217799454 16-17 29.99078661761786 25.317950925911465 20.709251975969355 23.982010480501316 18-19 28.56260080622673 26.011631616402052 20.483490644959076 24.942276932412145 20-21 37.96303938999824 23.626414075728203 17.062046441693568 21.348500092579993 22-23 33.89509682974874 26.39890599449874 16.507012655573426 23.198984520179092 24-25 26.94914346775651 28.91462786189296 18.86044068076411 25.275787989586423 26-27 23.81321004110361 29.788681728693888 20.693016848574757 25.70509138162775 28-29 24.366831614690934 29.022671801027172 22.05246507311109 24.558031511170803 30-31 25.053261381204255 29.819234776168347 19.261546286649963 25.86595755597743 32-33 22.77043954303408 30.57415211227388 19.98969350445388 26.665714840238163 34-35 22.69291774211899 31.091930817090933 20.51059538973946 25.704556051050613 36-37 22.296385364776324 31.772003364111534 19.386808131869603 26.54480313924254 38-39 22.566875101782223 30.15285737550222 20.27178362605714 27.00848389665842 40-41 21.55217607599144 30.494399468166982 21.864382580684023 26.089041875157555 42-43 20.724979420471556 30.650000780795118 23.234901095567093 25.390118703166237 44-45 21.157093746723447 30.263953366453915 23.186491798305006 25.392461088517628 46-47 19.15480043992228 30.347833070465647 22.976904080435283 27.520462409176798 48-49 18.686881080441974 30.607503217991304 23.679619685852664 27.02599601571406 50-51 19.349218424087752 30.001271580619328 22.926933192938936 27.722576802353988 52-53 19.97285063835578 28.36015178657077 22.319363049651876 29.347634525421572 54-55 19.421274659182934 28.19306163150486 22.08925157441758 30.296412134894624 56-57 20.50372551644135 27.387565252308928 22.853254807477803 29.25545442377192 58-59 21.93677344225797 25.64108856224387 22.36308757621118 30.059050419286976 60-61 21.77785015632651 25.416694087478152 22.38765182299132 30.41780393320402 62-63 22.48991101166508 24.599842502470658 22.612049676416195 30.29819680944807 64-65 22.9809438398765 24.037822306103234 22.817532024254106 30.16370182976616 66-67 23.846093784857004 24.923370365412932 22.408089055458884 28.822446794271183 68-69 23.947723325387273 24.749542924901363 22.65017663902836 28.65255711068301 70-71 23.814841755770157 23.8496501312008 23.7348494314632 28.600658681565843 72-73 24.872283942354883 22.521081515192076 24.574354169455226 28.032280372997814 74-75 25.435056155709944 21.539964927780677 24.982932412352916 28.04204650415646 76-77 25.45495512076179 21.19402086073103 25.611895814580205 27.739128203926978 78-79 26.27711905054043 21.80444166824687 26.115715385216003 25.8027238959967 80-81 27.107684516858015 22.489478727118392 25.233765784697894 25.169070971325695 82-83 26.153877042478648 21.76491125138173 25.948862324474277 26.13234938166535 84-85 26.097108998552994 21.784872363284627 26.615777841494175 25.5022407966682 86-87 26.64332380358531 22.006690530077826 25.851570177503174 25.498415488833686 88-89 26.671582895539803 22.11957146251859 25.359596854357275 25.84924878758433 90-91 27.341386695221527 22.214562976843798 25.04907865970642 25.394971668228262 92-93 27.274126829007084 23.432598383735616 24.54640178137146 24.746873005885845 94-95 26.21899249885095 24.444999754572756 23.837009535964015 25.49899821061227 96-97 25.459697030542355 25.26526560024987 23.30154161925797 25.973495749949805 98-99 24.929280535415504 26.860011154489683 23.0276631344116 25.183045175683212 100-101 24.382275386049997 28.835068197332784 22.648898625345225 24.13375779127199 102-103 23.477170666196702 30.829918284213885 22.540328067799788 23.152582981789628 104-105 22.56393896838773 32.60711708285044 22.216595521121135 22.612348427640686 106-107 21.851216881406067 33.76693924126031 21.903865309259317 22.477978568074306 108-109 21.832258438722082 35.304543558328746 21.56466879786553 21.298529205083646 110-111 22.00994509894905 36.98392231311669 20.40094944686243 20.60518314107183 112-113 21.935519132997367 37.50437806883105 20.203700109535376 20.35640268863621 114-115 21.83234649758831 37.76230718776353 20.567493764417694 19.83785255023046 116-117 21.996671581957834 38.069115983768384 20.072011618231343 19.86220081604244 118-119 22.390518648161752 38.109020365975894 20.08235238964802 19.418108596214335 120-121 22.829862466676335 37.38340899711102 20.207248106546498 19.579480429666148 122-123 23.68121632625743 37.52038551206399 19.88488181422691 18.913516347451672 124-125 24.631831740509018 38.700264302995876 18.51093659858591 18.15696735790919 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2.0 1 5.0 2 7.0 3 5.5 4 4.5 5 9.0 6 10.5 7 9.0 8 11.0 9 11.0 10 13.5 11 23.5 12 38.0 13 44.0 14 49.0 15 70.5 16 87.5 17 111.5 18 180.0 19 247.0 20 333.0 21 509.5 22 706.0 23 909.0 24 1228.5 25 1596.0 26 2007.0 27 2436.5 28 2823.5 29 3224.5 30 3627.5 31 4008.0 32 4313.5 33 4552.0 34 4691.5 35 4699.5 36 4669.0 37 4547.5 38 4344.5 39 4136.0 40 3840.0 41 3576.5 42 3316.0 43 3072.0 44 3003.5 45 3329.5 46 3997.0 47 4852.0 48 6042.5 49 8455.0 50 13307.0 51 20822.5 52 30556.0 53 39031.5 54 41348.0 55 38214.0 56 32577.0 57 25895.0 58 20066.0 59 15835.5 60 12823.5 61 10128.0 62 7679.0 63 5943.5 64 5001.5 65 4544.5 66 3833.0 67 3003.0 68 2582.0 69 2339.5 70 2035.5 71 1758.0 72 1432.0 73 1112.5 74 766.5 75 473.0 76 304.5 77 195.5 78 164.5 79 156.0 80 142.5 81 114.5 82 74.5 83 52.0 84 43.0 85 34.5 86 22.5 87 14.5 88 8.0 89 9.5 90 8.0 91 6.0 92 4.5 93 3.0 94 2.5 95 1.0 96 2.0 97 2.5 98 0.5 99 0.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14388938587117775 2 0.015838986661788557 3 0.0064694452562234946 4 0.01360814346998735 5 0.002677011830161446 6 0.0 7 0.0 8 4.46168638360241E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 7.807951171304217E-4 28-29 0.0087002884480247 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 2.230843191801205E-4 58-59 0.0 60-61 1.1154215959006025E-4 62-63 0.0 64-65 0.0015615902342608434 66-67 2.230843191801205E-4 68-69 0.006804071734993675 70-71 0.020746841683751207 72-73 2.230843191801205E-4 74-75 0.009592625724745181 76-77 5.577107979503013E-4 78-79 0.001338505915080723 80-81 5.577107979503013E-4 82-83 1.1154215959006025E-4 84-85 0.021081468162521388 86-87 0.0034578069472918677 88-89 0.019073709289900303 90-91 2.230843191801205E-4 92-93 0.014946649385068074 94-95 0.014054312108347592 96-97 0.006915613894583735 98-99 0.0024539275109813257 100-101 6.692529575403615E-4 102-103 0.0 104-105 3.3462647877018076E-4 106-107 0.0010038794363105422 108-109 0.0 110-111 0.0 112-113 8.92337276720482E-4 114-115 0.006023276617863254 116-117 0.0 118-119 0.06870997030747712 120-121 0.001338505915080723 122-123 0.005800192298683134 124-125 0.23356928218158618 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 448261.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.25462934538039 #Duplication Level Percentage of deduplicated Percentage of total 1 85.24805866445439 53.92334353233306 2 7.197247680662479 9.105184686944076 3 2.4223907624049112 4.596822894167883 4 1.3366451988783894 3.3819598648533913 5 0.8567985151641042 2.709823625018884 6 0.5621053272768706 2.1333458477977305 7 0.41200077649544015 1.8242669485059582 8 0.30350177956724034 1.5358314057751308 9 0.2462099950213836 1.4016529778585016 >10 1.3605155790745647 14.61523637546282 >50 0.03869567299528035 1.602785662894877 >100 0.013719374937675265 1.6837816308781623 >500 0.001055336533667328 0.5527307481302175 >1k 0.001055336533667328 0.9332337993792801 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT 1442 0.32168758825773375 No Hit ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA 1427 0.31834132347003197 No Hit GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT 1325 0.29558672291365967 No Hit TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC 860 0.19185251449490365 No Hit GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA 815 0.1818137201317982 No Hit ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC 809 0.1804752142167175 No Hit GGGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGA 450 0.10038794363105422 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 1.1154215959006025E-4 0.0 0.0 0.0 0.0 12-13 2.230843191801205E-4 0.0 0.0 2.230843191801205E-4 0.0 14-15 2.230843191801205E-4 0.0 0.0 6.692529575403615E-4 0.0 16-17 2.230843191801205E-4 0.0 0.0 0.0013385059150807233 0.0 18-19 4.46168638360241E-4 0.0 0.0 0.001784674553440964 0.0 20-21 7.807951171304218E-4 0.0 0.0 0.005130939341142771 0.0 22-23 8.92337276720482E-4 0.0 0.0 0.019966046566620786 0.0 24-25 8.92337276720482E-4 0.0 0.0 0.05242481500732832 0.0 26-27 8.92337276720482E-4 0.0 0.0 0.12916582080528977 0.0 28-29 0.0011154215959006024 0.0 0.0 0.40266719612011753 0.0 30-31 0.001338505915080723 0.0 0.0 0.9468813927600215 0.0 32-33 0.0015615902342608436 0.0 0.0 1.7407269425624805 0.0 34-35 0.0020077588726210844 0.0 0.0 2.8988691855860758 0.0 36-37 0.0022308431918012053 0.0 0.0 4.496710621713689 0.0 38-39 0.0022308431918012053 0.0 0.0 6.466098991435793 0.0 40-41 0.0022308431918012053 0.0 0.0 8.537325352863622 0.0 42-43 0.0029000961493415665 0.0 0.0 10.194730302212328 0.0 44-45 0.0031231804685216873 0.0 0.0 11.694749264379457 0.0 46-47 0.0031231804685216873 0.0 0.0 13.51567501968719 0.0 48-49 0.0031231804685216873 0.0 0.0 15.529345626766549 0.0 50-51 0.0031231804685216873 0.0 0.0 17.373025982630654 0.0 52-53 0.0031231804685216873 0.0 0.0 18.9749944786631 0.0 54-55 0.0031231804685216873 0.0 0.0 20.57015890296055 0.0 56-57 0.0032347226281117473 0.0 0.0 22.282888763465927 0.0 58-59 0.0033462647877018077 0.0 0.0 23.93237421948374 0.0 60-61 0.0033462647877018077 0.0 0.0 25.612979938027177 0.0 62-63 0.0033462647877018077 0.0 0.0 27.194982387493 0.0 64-65 0.003569349106881928 0.0 0.0 28.700243831160865 0.0 66-67 0.003569349106881928 0.0 0.0 30.35809494914793 0.0 68-69 0.003569349106881928 0.0 0.0 32.10216815649811 0.0 70-71 0.003569349106881928 0.0 0.0 33.981430461271444 0.0 72-73 0.003569349106881928 0.0 0.0 35.82667240736981 0.0 74-75 0.003569349106881928 0.0 0.0 37.437118107531106 0.0 76-77 0.003569349106881928 0.0 0.0 39.12330093405404 0.0 78-79 0.003569349106881928 0.0 0.0 40.86413941877612 0.0 80-81 0.003569349106881928 0.0 0.0 42.518086561177526 0.0 82-83 0.003569349106881928 0.0 0.0 44.28435665828613 0.0 84-85 0.003569349106881928 0.0 0.0 46.17120382991159 0.0 86-87 0.003569349106881928 0.0 0.0 47.67546139414314 0.0 88-89 0.003569349106881928 0.0 0.0 49.02244451335271 0.0 90-91 0.003569349106881928 0.0 0.0 50.80299200688884 0.0 92-93 0.003569349106881928 0.0 0.0 52.29018362070312 0.0 94-95 0.003569349106881928 0.0 0.0 53.62177392188926 0.0 96-97 0.003569349106881928 0.0 0.0 54.944552392467784 0.0 98-99 0.003569349106881928 0.0 0.0 56.09109425089401 0.0 100-101 0.003569349106881928 0.0 0.0 57.196923221069866 0.0 102-103 0.003569349106881928 0.0 0.0 58.207383644796224 0.0 104-105 0.003569349106881928 0.0 0.0 59.1659769643132 0.0 106-107 0.003569349106881928 0.0 0.0 60.040579037658866 0.0 108-109 0.003569349106881928 0.0 0.0 60.859298489049905 0.0 110-111 0.003569349106881928 0.0 0.0 61.69497234869864 0.0 112-113 0.003569349106881928 0.0 0.0 62.518041944313694 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAACAAC 215 0.0 63.749233 1 TACGGGC 65 5.474776E-7 54.92179 4 GTGTACG 125 0.0 52.440674 1 GTCTAAG 60 2.1713602E-5 49.659725 1 GTATGAG 90 9.58953E-8 46.34908 1 GTTCTAG 65 3.484889E-5 45.839752 1 TGGAATC 355 0.0 43.57644 2 CTATAGA 55 8.4584655E-4 43.3394 1 GTGGAAT 405 0.0 42.670586 1 ATGGGAC 70 5.4473836E-5 42.499004 5 TATAGAG 70 5.4473836E-5 42.499004 2 ATATTAT 30 0.0012927948 39.763325 118-119 TATAGAA 60 0.0013078096 39.665737 2 GCGTTAT 65 0.0019230461 36.671803 1 TATACGG 65 0.0019378623 36.61453 2 TGTAAGG 120 9.2733353E-7 34.70752 2 AGTCGTC 155 5.1113602E-9 34.54372 7 GTATACG 70 0.0027662185 34.052387 1 CGTTATA 70 0.0027875006 33.999203 2 TGTACGG 175 4.4383341E-10 33.999203 2 >>END_MODULE