FastQCFastQC Report
Thu 26 May 2016
SRR935991_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935991_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448261
Sequences flagged as poor quality0
Sequence length125
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT90482.0184669199417304No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT51501.1488842437776208RNA PCR Primer, Index 23 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT28250.6302132016838404RNA PCR Primer, Index 23 (96% over 25bp)
ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA13990.3120949625329886No Hit
GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT12950.28889419333825606No Hit
GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT12790.2853248442313741No Hit
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG12390.2764014714641693RNA PCR Primer, Index 23 (96% over 30bp)
CATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCT11870.26480108686680304RNA PCR Primer, Index 23 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC9090.20278364613472954No Hit
TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC8970.2001066343045681No Hit
ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC8870.19787579111276687No Hit
GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA8270.18449073196195967No Hit
CACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTG8240.1838214790044193Illumina PCR Primer Index 1 (95% over 23bp)
TATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCT5280.11778852052710363RNA PCR Primer, Index 23 (95% over 22bp)
GGTATCAACGCAGAGTGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5180.11555767733530242No Hit
GTATCAACGCAGAGTGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4910.10953440071743918No Hit
GGGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGA4900.10931131639825904No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTACA950.069.1202241
GTTAGCG359.0846406E-568.093013
GTATACG401.7424254E-459.694741
TATATGG401.7588817E-459.5813832
TATACGG401.7588817E-459.5813832
GTAAATT300.00421200659.5813833
GTTACAG655.3517397E-755.1028371
TTACAGG655.422462E-754.9981962
GTTCTAG453.1181163E-453.061991
GTATATG453.1181163E-453.061991
GTGTAGG2050.052.414891
TCAGATG1101.7462298E-1048.74844
GTATTGG1251.0913936E-1147.755791
TATCACA505.2933046E-447.66513
ACTAGGG802.2692911E-644.6860353
TACTAGG802.2692911E-644.6860352
GTACGAG951.4689249E-743.902073
TACATGG1501.8189894E-1243.693012
GATGTGT1501.8189894E-1243.6296547
ATATGGG558.4656675E-443.3319133
Produced by FastQC (version 0.11.5)