##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935991_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 448261 Sequences flagged as poor quality 0 Sequence length 125 %GC 50 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.29541717883108 33.0 31.0 34.0 30.0 34.0 2 32.3918699150718 34.0 31.0 34.0 30.0 34.0 3 32.38372064489215 34.0 31.0 34.0 30.0 34.0 4 35.87930915248036 37.0 35.0 37.0 35.0 37.0 5 35.8764692890972 37.0 35.0 37.0 35.0 37.0 6 35.965415237997505 37.0 35.0 37.0 35.0 37.0 7 35.94481340112122 37.0 35.0 37.0 35.0 37.0 8 35.98798690941215 37.0 35.0 37.0 35.0 37.0 9 37.64607226593436 39.0 37.0 39.0 35.0 39.0 10-11 37.58859124483281 39.0 37.0 39.0 35.0 39.0 12-13 37.549070742268455 39.0 37.0 39.0 35.0 39.0 14-15 38.632824403639844 40.0 38.0 41.0 34.0 41.0 16-17 38.59793066985529 40.0 38.0 41.0 34.0 41.0 18-19 38.69076720928209 40.0 38.0 41.0 34.0 41.0 20-21 38.801481726047996 40.0 38.0 41.0 35.0 41.0 22-23 38.60250947550646 40.0 38.0 41.0 34.0 41.0 24-25 38.241709628988474 40.0 37.0 41.0 33.5 41.0 26-27 38.02602167041077 40.0 37.0 41.0 33.0 41.0 28-29 37.842434429941484 40.0 36.5 41.0 33.0 41.0 30-31 37.559128498798685 40.0 35.5 41.0 33.0 41.0 32-33 37.039742471461935 39.0 35.0 41.0 32.5 41.0 34-35 36.6155844474536 39.0 35.0 41.0 31.5 41.0 36-37 36.24477146126921 39.0 35.0 41.0 31.0 41.0 38-39 35.5532747662634 39.0 35.0 41.0 29.0 41.0 40-41 35.16143942926108 39.0 35.0 41.0 27.5 41.0 42-43 34.70684935785179 38.0 35.0 41.0 25.0 41.0 44-45 34.04893019914738 38.0 35.0 41.0 21.0 41.0 46-47 33.24688072350706 37.0 34.0 40.0 5.0 41.0 48-49 32.20658946461994 36.5 33.0 40.0 2.0 41.0 50-51 31.071922384503672 36.0 32.0 39.0 2.0 41.0 52-53 30.441014275165585 35.0 31.5 39.0 2.0 41.0 54-55 29.97216800033909 35.0 31.0 38.5 2.0 40.5 56-57 29.555331603686245 35.0 30.5 37.0 2.0 40.0 58-59 29.256544736213947 35.0 30.0 37.0 2.0 39.0 60-61 28.83619364611242 35.0 29.5 36.0 2.0 39.0 62-63 28.446229986548015 35.0 29.0 35.5 2.0 38.0 64-65 28.11875224478596 35.0 29.0 35.0 2.0 37.0 66-67 27.63411160016151 35.0 27.5 35.0 2.0 36.5 68-69 27.105740851869783 35.0 25.5 35.0 2.0 36.0 70-71 26.550494020224825 35.0 22.0 35.0 2.0 35.5 72-73 26.209925690613282 35.0 20.0 35.0 2.0 35.0 74-75 26.085980042876805 34.0 19.0 35.0 2.0 35.0 76-77 25.95275854914882 34.0 19.0 35.0 2.0 35.0 78-79 25.976638610095456 34.0 19.0 35.0 2.0 35.0 80-81 25.927373784469317 34.0 18.0 35.0 2.0 35.0 82-83 25.837642578765497 34.0 18.0 35.0 2.0 35.0 84-85 25.79204414392508 34.0 18.0 35.0 2.0 35.0 86-87 25.76301195062698 34.0 17.0 35.0 2.0 35.0 88-89 25.681202469097244 34.0 16.0 35.0 2.0 35.0 90-91 25.56761239545711 34.0 13.5 35.0 2.0 35.0 92-93 25.487146996950436 34.0 10.0 35.0 2.0 35.0 94-95 25.46010025409304 34.0 10.0 35.0 2.0 35.0 96-97 25.426577373449845 34.0 7.5 35.0 2.0 35.0 98-99 25.33663646848599 34.0 7.0 35.0 2.0 35.0 100-101 25.21545260462097 34.0 3.5 35.0 2.0 35.0 102-103 24.515031421426357 32.5 2.0 35.0 2.0 35.0 104-105 24.89786976783615 33.5 2.0 35.0 2.0 35.0 106-107 24.76158867267061 34.0 2.0 35.0 2.0 35.0 108-109 24.454797539826128 34.0 2.0 35.0 2.0 35.0 110-111 24.017589083145758 34.0 2.0 35.0 2.0 35.0 112-113 23.473997291756366 33.0 2.0 35.0 2.0 35.0 114-115 22.815456843223032 33.0 2.0 35.0 2.0 35.0 116-117 22.20711482819161 33.0 2.0 35.0 2.0 35.0 118-119 21.678488648354417 33.0 2.0 35.0 2.0 35.0 120-121 21.06661632397197 32.0 2.0 35.0 2.0 35.0 122-123 20.381151828956792 31.0 2.0 35.0 2.0 35.0 124-125 18.375149745349248 25.5 2.0 33.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.23830352597999394 1101 2 0.15844634347048014 1101 3 0.24637344434885478 1101 4 0.335269298605823 1101 5 0.3108623993399675 1101 6 0.2294898464081001 1101 7 0.3484804262062582 1101 8 0.11375795818681667 1101 9 0.37005376125588185 1101 10-11 0.37641211126192786 1101 12-13 0.31859502328295974 1101 14-15 0.4350600991503626 1101 16-17 0.07438608301947625 1101 18-19 0.2548566778290393 1101 20-21 0.21868154740503343 1101 22-23 0.3739435008128922 1101 24-25 0.6692872842364963 1101 26-27 0.3336442710547658 1101 28-29 0.3800242765316497 1101 30-31 0.3960196514886434 1101 32-33 0.4964009319343887 1101 34-35 0.6799047363298172 1101 36-37 0.7190352572859382 1101 38-39 0.5941760312310151 1101 40-41 0.8279239504528135 1101 42-43 0.7723752805589754 1101 44-45 0.8476649575780186 1101 46-47 0.9765013493389105 1101 48-49 0.727257331942404 1101 50-51 0.7673640287152601 1101 52-53 0.8294483614171462 1101 54-55 0.8306887736786628 1101 56-57 0.7514448640852933 1101 58-59 0.5843771266732638 1101 60-61 0.5760406172513513 1101 62-63 0.6686443069522667 1101 64-65 0.8434654490438369 1101 66-67 0.40397277896522255 1101 68-69 0.6011339475514781 1101 70-71 0.5870957760010462 1101 72-73 0.387079627534721 1101 74-75 0.35865208391247094 1101 76-77 0.453487404859203 1101 78-79 0.49545505837446413 1101 80-81 0.5160834174252749 1101 82-83 0.5858413235212083 1101 84-85 0.5037990394846545 1101 86-87 0.5406226980839399 1101 88-89 0.34837688203845474 1101 90-91 0.31372620897427694 1101 92-93 0.4462874299781454 1101 94-95 0.38424453645884427 1101 96-97 0.5858203521651113 1101 98-99 0.432938361643469 1101 100-101 0.44976384138361425 1101 102-103 0.46482016768500145 1101 104-105 0.4495676693749431 1101 106-107 0.41281450930682695 1101 108-109 0.43104112228290603 1101 110-111 0.5340341358342968 1101 112-113 0.3818498205624081 1101 114-115 0.3324669179605486 1101 116-117 0.0814084655399192 1101 118-119 -0.09994844159743366 1101 120-121 0.22110571570038218 1101 122-123 -0.05405297800122 1101 124-125 -0.07338954600604097 1102 1 0.17849652418465922 1102 2 0.19498663283577855 1102 3 0.25422472220290615 1102 4 0.3062667640192771 1102 5 0.2156899946509796 1102 6 0.3121411295425389 1102 7 0.3154944984419785 1102 8 0.3817211802799605 1102 9 0.3180683351285225 1102 10-11 0.22780382369197127 1102 12-13 0.32757828445098625 1102 14-15 0.45774596883037333 1102 16-17 0.5164651700402842 1102 18-19 0.42410798472522515 1102 20-21 0.6206148296946168 1102 22-23 0.5587521527296744 1102 24-25 0.6099373660825194 1102 26-27 0.7602567697346672 1102 28-29 0.7895266659075091 1102 30-31 0.6344308485527463 1102 32-33 0.910662271568988 1102 34-35 0.9998236823642515 1102 36-37 0.8754585200380802 1102 38-39 1.221664414376022 1102 40-41 1.0171928806460784 1102 42-43 1.0739382755954097 1102 44-45 1.231458454779414 1102 46-47 1.3260498761104813 1102 48-49 1.0368415673421296 1102 50-51 1.048744388309725 1102 52-53 1.1524711858970278 1102 54-55 1.1133743793392377 1102 56-57 1.267673808535605 1102 58-59 1.1258661302639261 1102 60-61 1.1320358384996325 1102 62-63 1.2567426409896498 1102 64-65 1.282821835673893 1102 66-67 1.5090475624692878 1102 68-69 1.6064011352164904 1102 70-71 1.5601179404323489 1102 72-73 1.587085897996225 1102 74-75 1.6028717084768402 1102 76-77 1.667980091520942 1102 78-79 1.6958359064048807 1102 80-81 1.651789405385987 1102 82-83 1.6909656106687407 1102 84-85 1.6864113797528972 1102 86-87 1.5711647959823445 1102 88-89 1.599960998629438 1102 90-91 1.5877222750847402 1102 92-93 1.7697805390709753 1102 94-95 1.6242183985350955 1102 96-97 1.7365688472543503 1102 98-99 1.8879511005810556 1102 100-101 1.856441222838889 1102 102-103 1.773260868920616 1102 104-105 1.6342294604827678 1102 106-107 1.6603508779835643 1102 108-109 1.3813262632692798 1102 110-111 1.4526732476729265 1102 112-113 1.5716649409552659 1102 114-115 1.2365866111699688 1102 116-117 1.3885884739885448 1102 118-119 1.1349908999381029 1102 120-121 1.191621345650745 1102 122-123 1.227713510012297 1102 124-125 1.0318799518289978 1103 1 -0.07462382444735738 1103 2 -0.07594279680100868 1103 3 -0.04970812930624646 1103 4 -0.0902837431236918 1103 5 -0.004515931479545543 1103 6 0.1126597810486274 1103 7 0.049690204989566666 1103 8 -0.01872381657436506 1103 9 0.146096999063829 1103 10-11 0.21510146520863316 1103 12-13 0.19323680307179814 1103 14-15 0.028429882961326314 1103 16-17 0.22944987135532813 1103 18-19 0.23482933997081545 1103 20-21 0.15279730909432487 1103 22-23 0.18117232182847687 1103 24-25 0.3323544662374971 1103 26-27 0.3909815008184623 1103 28-29 0.4880198144904213 1103 30-31 0.3366635051839708 1103 32-33 0.5655641420902455 1103 34-35 0.5581412873604847 1103 36-37 0.45279436990534805 1103 38-39 0.723229575725739 1103 40-41 0.6895064039470284 1103 42-43 0.7897709567781774 1103 44-45 0.9165265086086904 1103 46-47 0.812285663064408 1103 48-49 0.9498707683425991 1103 50-51 1.0772763590320018 1103 52-53 0.887682712850026 1103 54-55 1.028149809374991 1103 56-57 0.9331175010888089 1103 58-59 1.2244145198356584 1103 60-61 1.26069056697483 1103 62-63 1.2031481405369817 1103 64-65 1.2194788351675179 1103 66-67 1.3966214089300273 1103 68-69 1.488351142121612 1103 70-71 1.4277804793695736 1103 72-73 1.4454679508127484 1103 74-75 1.2481647158883362 1103 76-77 1.1368364496053047 1103 78-79 1.394678914583615 1103 80-81 1.2468023716736383 1103 82-83 1.4797562933552975 1103 84-85 1.7630781998667544 1103 86-87 1.8731657473549284 1103 88-89 1.5679595869152756 1103 90-91 1.5807542381346558 1103 92-93 1.3092870529042386 1103 94-95 1.4679109517429083 1103 96-97 1.644813564834795 1103 98-99 1.4968602444991745 1103 100-101 1.4623596810015158 1103 102-103 1.3871555120791683 1103 104-105 1.5334100524820329 1103 106-107 1.5059753313279423 1103 108-109 1.4650531886479143 1103 110-111 1.260853517433091 1103 112-113 1.1189711755146448 1103 114-115 0.7329115509419459 1103 116-117 1.0377621608642045 1103 118-119 0.9927944568439955 1103 120-121 1.0612326639155647 1103 122-123 0.6305231280822348 1103 124-125 0.5053796219175375 1104 1 0.01622905789488982 1104 2 0.07593936359178599 1104 3 0.09668212346698368 1104 4 0.1008055458379431 1104 5 0.19708435948245295 1104 6 0.06701511838268459 1104 7 -0.12539617435213302 1104 8 -0.007339928594738865 1104 9 0.05593515145517358 1104 10-11 0.16214638246632518 1104 12-13 0.13639010367190707 1104 14-15 0.14110491478528786 1104 16-17 0.15325419941497387 1104 18-19 0.12446381896700132 1104 20-21 0.09791460314779243 1104 22-23 0.2701885065893421 1104 24-25 0.11245993995999726 1104 26-27 0.32756997144948485 1104 28-29 0.29796707762917407 1104 30-31 0.27959858428925344 1104 32-33 0.3506523399444035 1104 34-35 0.39865774489822314 1104 36-37 0.20349449720121981 1104 38-39 0.40762673884630374 1104 40-41 0.5519359365893308 1104 42-43 0.24065293531973708 1104 44-45 0.3252917386505132 1104 46-47 0.5318710422242461 1104 48-49 0.7544352346091969 1104 50-51 0.5836593377554031 1104 52-53 0.6951022436738121 1104 54-55 0.6505356977183254 1104 56-57 0.6832902597722068 1104 58-59 0.5854256127616431 1104 60-61 0.5136820199662822 1104 62-63 0.554612043064715 1104 64-65 0.6559218248019967 1104 66-67 0.7644519235690552 1104 68-69 0.7343413221543464 1104 70-71 0.4966657312335485 1104 72-73 0.5375937918747624 1104 74-75 0.541017961933072 1104 76-77 0.5045578534969266 1104 78-79 0.6767483817594666 1104 80-81 0.6267987346487267 1104 82-83 0.751223832907943 1104 84-85 0.798575563023693 1104 86-87 0.7281211937997369 1104 88-89 0.7628131966625062 1104 90-91 0.6548768058742489 1104 92-93 0.5890470092599394 1104 94-95 0.8061360835850628 1104 96-97 0.6994780492865154 1104 98-99 0.8842943234226155 1104 100-101 0.7581225469771447 1104 102-103 0.7343277252088285 1104 104-105 0.8157886667755392 1104 106-107 0.9222328326918294 1104 108-109 0.8876936687352028 1104 110-111 1.137231040619124 1104 112-113 1.0187893242689618 1104 114-115 0.8340208435405998 1104 116-117 0.8672766180382361 1104 118-119 0.6725180429505571 1104 120-121 0.6713981485491338 1104 122-123 0.5799321115337754 1104 124-125 0.00574332440889691 1105 1 0.1749957715373469 1105 2 0.26326436114604945 1105 3 0.3000564482450798 1105 4 0.28486779713286126 1105 5 0.34418820947015405 1105 6 0.28103170709421477 1105 7 0.2818733512276381 1105 8 0.3058995765466008 1105 9 0.4661527824475584 1105 10-11 0.3601189320354763 1105 12-13 0.3529899201754816 1105 14-15 0.32318016947252204 1105 16-17 0.3194281931591689 1105 18-19 0.42156145545899903 1105 20-21 0.4570017745265744 1105 22-23 0.20392752390516478 1105 24-25 0.3317275935589308 1105 26-27 0.2455663702660189 1105 28-29 0.4809267639401824 1105 30-31 0.4395299978727536 1105 32-33 0.5826153551026891 1105 34-35 0.3994417029743147 1105 36-37 0.5315842570958864 1105 38-39 0.3694124181929084 1105 40-41 0.27445179422359445 1105 42-43 0.4253259600230095 1105 44-45 0.08949089837437185 1105 46-47 0.1407724697418402 1105 48-49 0.08551276052740064 1105 50-51 0.22973068983851164 1105 52-53 -0.11142668609782191 1105 54-55 0.04234419513641896 1105 56-57 0.01476835786083086 1105 58-59 0.05370770017891502 1105 60-61 0.22500300915480054 1105 62-63 0.3038882369152027 1105 64-65 0.3097402162204972 1105 66-67 0.375266314553933 1105 68-69 0.024836788946519306 1105 70-71 -0.07321229916762917 1105 72-73 0.0643832125389956 1105 74-75 0.029930177136833436 1105 76-77 0.06507333567741114 1105 78-79 0.10629262845904108 1105 80-81 -9.269408375764954E-4 1105 82-83 0.02309335478605945 1105 84-85 -0.07093309488767474 1105 86-87 -0.052157699616333986 1105 88-89 -0.00952960850606388 1105 90-91 0.03811861133101857 1105 92-93 0.13355282482752173 1105 94-95 0.22926853151729176 1105 96-97 0.1704504481453455 1105 98-99 0.10022519762180337 1105 100-101 0.18990894021474958 1105 102-103 0.20523402304944227 1105 104-105 0.12028300720635698 1105 106-107 0.31381262058688364 1105 108-109 0.3565614111739066 1105 110-111 0.4865815320140925 1105 112-113 0.6295230720116152 1105 114-115 0.5567865630237421 1105 116-117 0.5504584037704632 1105 118-119 0.41969751250310594 1105 120-121 0.33273619180035396 1105 122-123 0.31500319656877096 1105 124-125 0.10601063692658386 1106 1 0.15932000949647573 1106 2 0.210713404140904 1106 3 0.21542356439687183 1106 4 0.1899534799706757 1106 5 0.20483344929841252 1106 6 0.2715643685246434 1106 7 0.1784883909306103 1106 8 0.13341293556238298 1106 9 0.4958964060060893 1106 10-11 0.2932629862273046 1106 12-13 0.2725669966877078 1106 14-15 0.3670309644195484 1106 16-17 0.37527230289204283 1106 18-19 0.45717366231514234 1106 20-21 0.282990925002224 1106 22-23 0.29542315036501776 1106 24-25 0.3453668506784524 1106 26-27 0.3227100896899131 1106 28-29 0.42013214660276077 1106 30-31 0.3504259332321098 1106 32-33 0.4083863991982213 1106 34-35 0.5755841619462245 1106 36-37 0.9007622750161062 1106 38-39 0.7480856720104043 1106 40-41 0.6422362138197002 1106 42-43 0.6641473740117405 1106 44-45 0.6802802152195397 1106 46-47 0.8442061235562974 1106 48-49 0.5655344697205891 1106 50-51 0.39093412983262965 1106 52-53 0.20819553336285068 1106 54-55 0.3272951085202891 1106 56-57 0.397342165313475 1106 58-59 0.33219404190089463 1106 60-61 0.403006980719816 1106 62-63 0.5108230053547018 1106 64-65 0.5865641308242431 1106 66-67 0.5590843428215351 1106 68-69 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-1.4446906759977907 2216 36-37 -1.7487780342986383 2216 38-39 -2.0264790489828854 2216 40-41 -2.250935464126414 2216 42-43 -1.9547215390301034 2216 44-45 -1.6542480384816542 2216 46-47 -1.910601099296045 2216 48-49 -2.164625407995679 2216 50-51 -2.2262109431395096 2216 52-53 -2.2420713050618843 2216 54-55 -2.5694660250829493 2216 56-57 -2.338448359950732 2216 58-59 -1.930948830264537 2216 60-61 -1.9227368243472966 2216 62-63 -2.097534802251225 2216 64-65 -2.1855021487608326 2216 66-67 -2.286882660100357 2216 68-69 -2.272423609442612 2216 70-71 -2.182575100722847 2216 72-73 -1.8973499038472106 2216 74-75 -1.8381314440920349 2216 76-77 -1.9240016888644078 2216 78-79 -2.0325406494780545 2216 80-81 -2.15430076495581 2216 82-83 -2.2418790888063995 2216 84-85 -2.2380300486099145 2216 86-87 -2.3639605766135006 2216 88-89 -2.2519960422508767 2216 90-91 -2.181920075855448 2216 92-93 -2.569289195408853 2216 94-95 -2.57510319785019 2216 96-97 -2.5007790004946138 2216 98-99 -2.8554227064679836 2216 100-101 -2.6407186733926302 2216 102-103 -2.5559721205221457 2216 104-105 -2.865289493585937 2216 106-107 -2.822941366009804 2216 108-109 -2.8288800710748205 2216 110-111 -2.565925405398467 2216 112-113 -2.313605604430556 2216 114-115 -2.1920414200636564 2216 116-117 -2.1306834286340184 2216 118-119 -1.9110016008994322 2216 120-121 -1.9990779319213452 2216 122-123 -1.9784522532615654 2216 124-125 -2.1427719341287563 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 12.0 4 36.0 5 147.0 6 437.0 7 1013.0 8 1618.0 9 2387.0 10 3749.0 11 7726.0 12 11455.0 13 10567.0 14 13603.0 15 18369.0 16 8280.0 17 3226.0 18 3299.0 19 4609.0 20 5663.0 21 7361.0 22 6648.0 23 2308.0 24 2803.0 25 3570.0 26 4671.0 27 5355.0 28 6585.0 29 8046.0 30 10308.0 31 14024.0 32 18661.0 33 24826.0 34 33695.0 35 50940.0 36 107726.0 37 39988.0 38 4524.0 39 19.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.45048029000666 20.61000630254918 12.238790625740325 15.700722781703833 2 19.79007718734663 21.669566769285684 37.64132423147281 20.899031811894883 3 28.35357079915496 22.71288378868561 29.705015604748127 19.228529807411306 4 15.165733887789603 23.695412335907594 27.513478022002563 33.62537575430024 5 14.575183380903391 34.59321462762575 28.69601504984256 22.1355869416283 6 30.219406364680818 36.03663093551662 10.0554371953464 23.688525504456166 7 26.008954604571887 40.68611813207038 12.836271725624135 20.468655537733596 8 34.17964087886298 27.834230504103637 13.348696406780872 24.63743221025251 9 31.48835165227401 13.947677803779495 18.925804386283886 35.63816615766261 10-11 29.247469666109698 24.196506053393 19.97128904812152 26.584735232375785 12-13 32.93616888375299 25.458159420516175 20.952636968194867 20.653034727535964 14-15 33.47491751457298 19.405881841159502 25.655031332192628 21.464169312074883 16-17 29.961674113964854 20.541938736584267 26.942562480340694 22.553824669110185 18-19 28.160714405223743 20.109489783853604 27.884201391599984 23.845594419322673 20-21 32.920441439250794 16.997686615610103 28.667004267603026 21.41486767753608 22-23 29.032416382420063 20.06364595626209 29.069783005882734 21.834154655435114 24-25 24.54507530211194 21.616089733436546 31.26560195957266 22.573233004878855 26-27 22.17748142265332 21.755517432924123 32.820945832896456 23.2460553115261 28-29 22.30352406299009 18.968190406928105 34.67154180265515 24.056743727426657 30-31 25.142272024557123 19.850042720647124 32.234345615612334 22.77333963918342 32-33 23.189280352294755 19.481507425361563 33.82917541343101 23.500036808912665 34-35 22.243068212492275 20.020144514021965 34.849340005041704 22.887447268444053 36-37 22.645400782133624 19.464999185742236 33.06711937911172 24.82248065301242 38-39 22.705187379673895 16.46496126140797 34.17573690327733 26.654114455640798 40-41 21.635944237843578 16.856585783728676 35.52517394999788 25.982296028429865 42-43 22.444736437031104 16.93633842783557 36.39419891536404 24.224726219769284 44-45 21.890371903868505 17.800009369541407 34.859155715085635 25.45046301150446 46-47 21.207733887177334 17.244752499102088 32.82574214575884 28.721771467961744 48-49 20.582763166994226 18.086115008889912 32.46869569291105 28.86242613120481 50-51 20.471890260361707 18.553142031093493 31.36598990320371 29.608977805341084 52-53 20.624368392521323 18.54700721231604 30.547493536131853 30.281130859030785 54-55 19.050954689343932 19.894771126642738 28.182576668503394 32.87169751550994 56-57 20.333131813831674 20.91605113984933 26.458246423400656 32.29257062291834 58-59 19.36829213337765 21.150289674988457 26.49538996254414 32.98602822908975 60-61 19.71708446641577 22.909421073883294 23.557927189739907 33.81556726996103 62-63 18.275625138033423 23.89634610193615 24.037781560296345 33.79024719973408 64-65 18.040048096979216 23.650730266518835 24.476253789644872 33.83296784685707 66-67 18.69468903161328 24.593149972895255 24.31050214049404 32.40165885499742 68-69 18.97465985218433 24.550429325772264 24.421040420647792 32.05387040139562 70-71 18.99752599490029 23.9900415159918 25.008979143847 32.00345334526091 72-73 19.406216467638274 22.95459564851727 25.283372856438547 32.35581502740591 74-75 19.947976736767195 22.570667535208283 25.02894519041362 32.4524105376109 76-77 20.348413089695512 22.43782082313652 25.478794720040334 31.734971367127635 78-79 20.91839352520072 22.71623005347331 25.794570573839792 30.570805847486177 80-81 20.649353836269498 23.165856498780844 25.033964587595175 31.150825077354487 82-83 19.64190505085207 22.654993407858367 25.58386743437417 32.11923410691539 84-85 20.302680804263588 22.240391200662113 25.68882860654842 31.768099388525883 86-87 21.129877459783476 22.351710275932994 24.925991777111996 31.592420487171534 88-89 20.907797020039666 22.257903319717755 24.578984118627318 32.25531554161527 90-91 20.981861014007464 21.608170240105654 24.43297543172393 32.976993314162954 92-93 20.936797981533083 21.6827919448714 24.25551185581614 33.124898217779375 94-95 20.846560374424723 21.52640983712614 24.010007562558418 33.61702222589072 96-97 21.298752289402824 21.69428078730918 23.703824334483706 33.30314258880429 98-99 21.716700761386782 22.32862104889785 23.499367555955125 32.45531063376024 100-101 22.200793734007643 23.092684842089763 22.76497397721417 31.941547446688425 102-103 21.67844180064739 24.260754337316875 22.332301940164324 31.72850192187141 104-105 21.387651390596105 26.105550114776882 21.911341829871436 30.595456664755577 106-107 21.316535046624725 27.850912416900908 21.61413019229911 29.218422344175256 108-109 21.28536723025202 28.7851274146089 21.283917182177348 28.645588172961734 110-111 21.254220537663315 29.766237706481473 21.09817079360051 27.881370962254703 112-113 21.419648015990756 30.47891955320473 21.19901663993361 26.902415790870904 114-115 20.916325995660976 31.003608417037082 21.392837822010787 26.687227765291155 116-117 20.47273781902552 31.52697215777262 21.69931286810637 26.300977155095485 118-119 20.248140839948825 31.964725317259095 22.22793575510286 25.55919808768922 120-121 20.61078664519094 32.27382444931267 22.319623966554975 24.795764938941414 122-123 20.210440132871984 32.37839960200869 22.388014750664247 25.02314551445507 124-125 19.452068550006693 33.45093051278618 22.741888695496943 24.35511224171018 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 2.5 4 4.0 5 2.5 6 3.0 7 4.0 8 7.5 9 14.0 10 15.5 11 19.0 12 22.5 13 24.0 14 28.0 15 30.5 16 52.0 17 66.5 18 73.5 19 109.5 20 153.0 21 200.5 22 287.5 23 441.5 24 648.5 25 901.5 26 1205.0 27 1633.5 28 2139.5 29 2688.0 30 3300.5 31 3950.5 32 4560.5 33 5213.5 34 5767.0 35 5981.0 36 5885.5 37 5609.5 38 5342.0 39 5018.0 40 4684.0 41 4463.0 42 4345.0 43 4355.5 44 4548.5 45 4971.0 46 5696.5 47 6569.0 48 7390.0 49 8903.0 50 12315.0 51 18153.0 52 27316.5 53 37148.5 54 40958.0 55 38359.0 56 33433.5 57 27535.5 58 21192.0 59 15907.0 60 12366.5 61 9389.5 62 6928.5 63 5291.0 64 4445.0 65 3989.5 66 3241.5 67 2472.0 68 2110.0 69 1881.0 70 1609.5 71 1476.0 72 1195.0 73 890.0 74 554.5 75 269.5 76 141.5 77 65.5 78 66.5 79 66.0 80 65.0 81 58.0 82 27.0 83 5.5 84 4.5 85 2.5 86 1.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1835983946852392 2 2.230843191801205E-4 3 0.0 4 0.11042673799415965 5 0.033462647877018074 6 0.001338505915080723 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 2.230843191801205E-4 108-109 0.0 110-111 0.0014500480746707835 112-113 4.46168638360241E-4 114-115 7.807951171304217E-4 116-117 0.0046847707027825305 118-119 0.0016731323938509038 120-121 6.692529575403615E-4 122-123 0.0022308431918012053 124-125 0.026993202620794583 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 448261.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.786410198124635 #Duplication Level Percentage of deduplicated Percentage of total 1 80.99445081776018 41.944118538203604 2 8.838509367107658 9.154293432500085 3 3.1936594049187055 4.961644679286562 4 1.6853872331811317 3.491206184008017 5 1.086599420743994 2.813554166184655 6 0.7777539649666821 2.4166251517789474 7 0.5838657417792871 2.1165417563090134 8 0.46560910572998115 1.9289779313051831 9 0.3264699307341314 1.521603517531596 >10 1.9322518587324873 17.671015694748228 >50 0.08529009164398828 2.961532081282479 >100 0.023691692122875856 2.5916751822705617 >500 0.0030153062701842 1.2023695328316688 >1k 0.002584548231586457 2.057635727428444 >5k 8.615160771954856E-4 3.167206424330989 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT 9048 2.0184669199417304 No Hit ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT 5150 1.1488842437776208 RNA PCR Primer, Index 23 (95% over 23bp) ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT 2825 0.6302132016838404 RNA PCR Primer, Index 23 (96% over 25bp) ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA 1399 0.3120949625329886 No Hit GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT 1295 0.28889419333825606 No Hit GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT 1279 0.2853248442313741 No Hit TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG 1239 0.2764014714641693 RNA PCR Primer, Index 23 (96% over 30bp) CATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCT 1187 0.26480108686680304 RNA PCR Primer, Index 23 (96% over 28bp) TTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC 909 0.20278364613472954 No Hit TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC 897 0.2001066343045681 No Hit ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC 887 0.19787579111276687 No Hit GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA 827 0.18449073196195967 No Hit CACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTG 824 0.1838214790044193 Illumina PCR Primer Index 1 (95% over 23bp) TATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCT 528 0.11778852052710363 RNA PCR Primer, Index 23 (95% over 22bp) GGTATCAACGCAGAGTGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 518 0.11555767733530242 No Hit GTATCAACGCAGAGTGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 491 0.10953440071743918 No Hit GGGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGA 490 0.10931131639825904 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 1.1154215959006025E-4 0.0 0.0 0.0 0.0 12-13 4.46168638360241E-4 0.0 0.0 2.230843191801205E-4 0.0 14-15 5.577107979503012E-4 0.0 0.0 6.692529575403615E-4 0.0 16-17 6.692529575403615E-4 0.0 0.0 0.0013385059150807233 0.0 18-19 6.692529575403615E-4 0.0 0.0 0.001784674553440964 0.0 20-21 7.807951171304218E-4 0.0 0.0 0.004907855021962651 0.0 22-23 8.92337276720482E-4 0.0 0.0 0.019519877928260546 0.0 24-25 8.92337276720482E-4 2.230843191801205E-4 0.0 0.05242481500732832 0.0 26-27 0.0011154215959006026 4.46168638360241E-4 0.0 0.1288311943265196 0.0 28-29 0.0011154215959006026 4.46168638360241E-4 0.0 0.40222102748175725 0.0 30-31 0.0011154215959006026 4.46168638360241E-4 0.0 0.9469929349196116 0.0 32-33 0.0011154215959006026 4.46168638360241E-4 0.0 1.741953906317971 0.0 34-35 0.0011154215959006026 4.46168638360241E-4 0.0 2.901323113097057 0.0 36-37 0.0015615902342608436 4.46168638360241E-4 0.0 4.501506934576062 0.0 38-39 0.0015615902342608436 4.46168638360241E-4 0.0 6.474018484766687 0.0 40-41 0.001784674553440964 4.46168638360241E-4 0.0 8.552829713046641 0.0 42-43 0.0020077588726210844 4.46168638360241E-4 0.0 10.21915803516255 0.0 44-45 0.0020077588726210844 4.46168638360241E-4 0.0 11.723304057234513 0.0 46-47 0.0020077588726210844 4.46168638360241E-4 0.0 13.547687619489539 0.0 48-49 0.0020077588726210844 4.46168638360241E-4 0.0 15.575412538677243 0.0 50-51 0.0020077588726210844 4.46168638360241E-4 0.0 17.431027905617487 0.0 52-53 0.0020077588726210844 4.46168638360241E-4 0.0 19.03823888315066 0.0 54-55 0.0020077588726210844 4.46168638360241E-4 0.0 20.64109971645983 0.0 56-57 0.0020077588726210844 4.46168638360241E-4 0.0 22.367326178275604 0.0 58-59 0.0020077588726210844 4.46168638360241E-4 0.0 24.03320833175315 0.0 60-61 0.0020077588726210844 4.46168638360241E-4 0.0 25.73467243413993 0.0 62-63 0.0022308431918012053 4.46168638360241E-4 0.0 27.355491555143097 0.0 64-65 0.0024539275109813257 4.46168638360241E-4 0.0 28.884623020963232 0.0 66-67 0.0024539275109813257 4.46168638360241E-4 0.0 30.55875929425045 0.0 68-69 0.0024539275109813257 4.46168638360241E-4 0.0 32.333060426849535 0.0 70-71 0.0024539275109813257 4.46168638360241E-4 0.0 34.24377762062727 0.0 72-73 0.0024539275109813257 4.46168638360241E-4 0.0 36.101958457238084 0.0 74-75 0.0024539275109813257 4.46168638360241E-4 0.0 37.73527030011533 0.0 76-77 0.0024539275109813257 4.46168638360241E-4 0.0 39.44331538991793 0.0 78-79 0.0024539275109813257 4.46168638360241E-4 0.0 41.21170478805874 0.0 80-81 0.0024539275109813257 4.46168638360241E-4 0.0 42.89275667524054 0.0 82-83 0.0024539275109813257 4.46168638360241E-4 0.0 44.683454505299366 0.0 84-85 0.0024539275109813257 4.46168638360241E-4 0.0 46.60064114433332 0.0 86-87 0.0024539275109813257 4.46168638360241E-4 0.0 48.130664947430176 0.0 88-89 0.0024539275109813257 4.46168638360241E-4 0.0 49.50486435357972 0.0 90-91 0.0024539275109813257 4.46168638360241E-4 0.0 51.31352047133255 0.0 92-93 0.0024539275109813257 4.46168638360241E-4 0.0 52.821682011149754 0.0 94-95 0.0024539275109813257 4.46168638360241E-4 0.0 54.1734614432217 0.0 96-97 0.0024539275109813257 4.46168638360241E-4 0.0 55.51464437013258 0.0 98-99 0.0024539275109813257 4.46168638360241E-4 0.0 56.681709985923376 0.0 100-101 0.0024539275109813257 4.46168638360241E-4 0.0 57.80661266538914 0.0 102-103 0.0025654696705713857 4.46168638360241E-4 0.0 58.837485304320474 0.0 104-105 0.002677011830161446 4.46168638360241E-4 0.0 59.81716009199997 0.0 106-107 0.002677011830161446 4.46168638360241E-4 0.0 60.715074476699954 0.0 108-109 0.002677011830161446 4.46168638360241E-4 0.0 61.55375997465762 0.0 110-111 0.002677011830161446 4.46168638360241E-4 0.0 62.409622965192156 0.0 112-113 0.002677011830161446 4.46168638360241E-4 0.0 63.24485065620252 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTACA 95 0.0 69.120224 1 GTTAGCG 35 9.0846406E-5 68.09301 3 GTATACG 40 1.7424254E-4 59.69474 1 TATATGG 40 1.7588817E-4 59.581383 2 TATACGG 40 1.7588817E-4 59.581383 2 GTAAATT 30 0.004212006 59.581383 3 GTTACAG 65 5.3517397E-7 55.102837 1 TTACAGG 65 5.422462E-7 54.998196 2 GTTCTAG 45 3.1181163E-4 53.06199 1 GTATATG 45 3.1181163E-4 53.06199 1 GTGTAGG 205 0.0 52.41489 1 TCAGATG 110 1.7462298E-10 48.7484 4 GTATTGG 125 1.0913936E-11 47.75579 1 TATCACA 50 5.2933046E-4 47.6651 3 ACTAGGG 80 2.2692911E-6 44.686035 3 TACTAGG 80 2.2692911E-6 44.686035 2 GTACGAG 95 1.4689249E-7 43.90207 3 TACATGG 150 1.8189894E-12 43.69301 2 GATGTGT 150 1.8189894E-12 43.629654 7 ATATGGG 55 8.4656675E-4 43.331913 3 >>END_MODULE