FastQCFastQC Report
Thu 26 May 2016
SRR935990_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935990_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences475646
Sequences flagged as poor quality0
Sequence length125
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA15250.32061659301245043No Hit
GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT14640.3077919292919524No Hit
GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT13920.2926546212939875No Hit
GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA10380.21822952363732695No Hit
ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC10260.21570663897099943No Hit
TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC9790.20582534069455016No Hit
GTATCAACGCAGAGTCGCCGGACAATTCACGCATTAACCGTTCATCCTTT7060.1484297145356No Hit
ACGCAGAGTCGCCGGACAATTCACGCATTAACCGTTCATCCTTTCCCGCA6220.13076952187130764No Hit
GGTATCAACGCAGAGTCGCCGGACAATTCACGCATTAACCGTTCATCCTT6050.12719543526067706No Hit
GGGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGA5790.12172918515030086No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGGGC250.00205878671.376544
CACTCGT150.00423302259.51172684-85
TAGAATC300.00424054359.4804422
GTTTACG453.1528185E-452.9438671
TTTACGG558.537169E-443.2585032
TATGGGT558.537169E-443.2585034
TAACAAC3550.041.9449651
GGAAACA6250.041.931541
GTGGAAT2250.037.0607071
TCTATAC650.00194088836.603353
GCAGAGA8300.035.114963
GTGTACG851.6985707E-435.0363851
GTATACG1407.975541E-834.0353431
TAGGGCT700.00279184633.9888274
TATACGG1408.072129E-833.9888272
AGTAAAG3500.033.9888272
GTATTAG902.3763499E-433.0899161
GTAACAA1451.0855547E-732.861711
TAAGTCA1102.0418567E-532.443885
GTACCAG1301.7192397E-632.0717621