SRR935990_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR935990_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	475646
Sequences flagged as poor quality	0
Sequence length	125
%GC	50

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT	8938	1.8791285956362505	No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT	5006	1.05246338663628	RNA PCR Primer, Index 39 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT	3020	0.6349259743590822	RNA PCR Primer, Index 39 (96% over 25bp)
ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA	1460	0.3069509677365099	No Hit
GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT	1430	0.3006437560706912	No Hit
GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT	1298	0.272892024741089	No Hit
ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC	1096	0.23042346619124307	No Hit
GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA	1072	0.2253776968585881	No Hit
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCT	1045	0.21970120635935128	RNA PCR Primer, Index 39 (96% over 28bp)
TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC	947	0.1990976482510102	No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG	923	0.19405187891835526	RNA PCR Primer, Index 39 (96% over 30bp)
TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC	860	0.18080673442013598	No Hit
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTG	803	0.16882303225508047	Illumina PCR Primer Index 11 (96% over 25bp)
ACGCAGAGTCGCCGGACAATTCACGCATTAACCGTTCATCCTTTCCCGCA	683	0.14359418559180567	No Hit
GGGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGA	662	0.13917913742573257	No Hit
GTATCAACGCAGAGTCGCCGGACAATTCACGCATTAACCGTTCATCCTTT	641	0.1347640892596595	No Hit
GGTATCAACGCAGAGTCGCCGGACAATTCACGCATTAACCGTTCATCCTT	624	0.13119000264902891	No Hit
GTATCAACGCAGAGTGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	609	0.12803639681611956	No Hit
TATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCT	579	0.12172918515030086	RNA PCR Primer, Index 39 (95% over 22bp)
GGTATCAACGCAGAGTGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	562	0.11815509853967025	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATAAT	30	0.0041801985	59.696095	1
TATTATG	30	0.0042169923	59.56415	2
TAGGGTG	30	0.004234598	59.501522	5
GTATTAG	45	3.11796E-4	53.0632	1
ATGACAG	35	0.0077926703	51.001305	5
TATGGGT	60	2.1775055E-5	49.63679	4
GTTCTAG	50	5.2436546E-4	47.75688	1
GTCTATG	50	5.2436546E-4	47.75688	1
GCCGTAC	50	5.3315196E-4	47.59622	7
GAGATGT	130	1.8189894E-11	45.920074	1
AGTCGCA	150	1.8189894E-12	43.629864	7
TATTAGG	55	8.478257E-4	43.31938	2
CAGATGT	110	9.607902E-9	43.273834	5
CGTACCG	55	8.526703E-4	43.269283	9
TATATGG	140	4.0017767E-11	42.545822	2
GCTTAAC	35	5.4538195E-5	42.49662	20-21
CGGAGAT	90	5.0340077E-6	39.7974	1
GTTACAG	75	8.129612E-5	39.709435	3
AGTTAGC	60	0.0013008283	39.709435	2
TAGGAGG	60	0.0013008283	39.709435	4

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content