##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935990_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 475646 Sequences flagged as poor quality 0 Sequence length 125 %GC 50 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.298364750255445 33.0 31.0 34.0 30.0 34.0 2 32.408841869793925 34.0 31.0 34.0 31.0 34.0 3 32.39667525849056 34.0 31.0 34.0 30.0 34.0 4 35.89314952716936 37.0 35.0 37.0 35.0 37.0 5 35.885767987116466 37.0 35.0 37.0 35.0 37.0 6 35.97717209857751 37.0 35.0 37.0 35.0 37.0 7 35.95777532030123 37.0 35.0 37.0 35.0 37.0 8 35.98684946367677 37.0 35.0 37.0 35.0 37.0 9 37.64920129676272 39.0 37.0 39.0 35.0 39.0 10-11 37.58408669472675 39.0 37.0 39.0 35.0 39.0 12-13 37.54680897137787 39.0 37.0 39.0 35.0 39.0 14-15 38.623312295278424 40.0 38.0 41.0 34.0 41.0 16-17 38.59243849417424 40.0 38.0 41.0 34.0 41.0 18-19 38.69258440100411 40.0 38.0 41.0 34.0 41.0 20-21 38.79644735790903 40.0 38.0 41.0 35.0 41.0 22-23 38.6233165000862 40.0 38.0 41.0 34.0 41.0 24-25 38.27599727528456 40.0 37.5 41.0 33.5 41.0 26-27 38.069725173763686 40.0 37.0 41.0 33.0 41.0 28-29 37.92433448404906 40.0 37.0 41.0 33.0 41.0 30-31 37.70648549551557 40.0 36.0 41.0 33.0 41.0 32-33 37.2531935515068 39.5 35.0 41.0 33.0 41.0 34-35 36.93868128818491 39.0 35.0 41.0 32.5 41.0 36-37 36.70536911905072 39.0 35.0 41.0 32.0 41.0 38-39 36.140652922551645 39.0 35.0 41.0 30.5 41.0 40-41 35.73148833376082 39.0 35.0 41.0 29.5 41.0 42-43 35.270286095121165 38.5 35.0 41.0 28.0 41.0 44-45 34.63961853983845 38.0 35.0 41.0 25.0 41.0 46-47 33.8741185671697 37.0 34.5 40.5 20.0 41.0 48-49 32.9063641868112 37.0 33.5 40.0 2.0 41.0 50-51 31.892472553117237 36.0 33.0 39.5 2.0 41.0 52-53 31.305352089579223 35.5 33.0 39.0 2.0 41.0 54-55 30.811459572875627 35.0 32.0 38.5 2.0 41.0 56-57 30.450887845162157 35.0 32.0 37.0 2.0 40.0 58-59 30.148276239051732 35.0 31.0 37.0 2.0 39.5 60-61 29.700340168949175 35.0 31.0 36.0 2.0 39.0 62-63 29.331119151637985 35.0 31.0 35.5 2.0 38.5 64-65 29.00147483632786 35.0 31.0 35.0 2.0 37.0 66-67 28.53495456705197 35.0 30.0 35.0 2.0 36.5 68-69 28.036533472372312 35.0 29.0 35.0 2.0 36.0 70-71 27.550692111360128 35.0 27.5 35.0 2.0 36.0 72-73 27.247547545863938 35.0 26.0 35.0 2.0 35.0 74-75 27.163690013161048 35.0 26.5 35.0 2.0 35.0 76-77 27.07790352489036 35.0 26.0 35.0 2.0 35.0 78-79 27.141145936263523 35.0 27.0 35.0 2.0 35.0 80-81 27.112747715738173 35.0 27.0 35.0 2.0 35.0 82-83 27.029599744347685 35.0 27.0 35.0 2.0 35.0 84-85 26.977626217817452 35.0 27.0 35.0 2.0 35.0 86-87 26.943405389722606 35.0 26.5 35.0 2.0 35.0 88-89 26.87329968085509 35.0 25.5 35.0 2.0 35.0 90-91 26.77822266980065 35.0 25.0 35.0 2.0 35.0 92-93 26.7043032002792 35.0 25.0 35.0 2.0 35.0 94-95 26.66230768260429 34.0 25.0 35.0 2.0 35.0 96-97 26.621097412781776 34.0 25.0 35.0 2.0 35.0 98-99 26.545072385765884 34.0 25.0 35.0 2.0 35.0 100-101 26.449716806196207 34.0 24.0 35.0 2.0 35.0 102-103 25.765393801272374 33.5 21.5 35.0 2.0 35.0 104-105 26.14205627714729 34.0 23.0 35.0 2.0 35.0 106-107 26.00725539581958 34.0 19.5 35.0 2.0 35.0 108-109 25.703993095705627 34.0 12.5 35.0 2.0 35.0 110-111 25.31542260420565 34.0 2.0 35.0 2.0 35.0 112-113 24.810876155796535 34.0 2.0 35.0 2.0 35.0 114-115 24.170776165467593 34.0 2.0 35.0 2.0 35.0 116-117 23.54503454249589 34.0 2.0 35.0 2.0 35.0 118-119 23.00690114076435 33.5 2.0 35.0 2.0 35.0 120-121 22.410168486647635 33.0 2.0 35.0 2.0 35.0 122-123 21.75037107428634 33.0 2.0 35.0 2.0 35.0 124-125 19.681577265445313 28.5 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1292252679763095 1101 2 0.23496470968171934 1101 3 0.17435551682897454 1101 4 0.16227535598580545 1101 5 0.21659142240203977 1101 6 0.25330129965462334 1101 7 0.34245782955973425 1101 8 0.2873052671468628 1101 9 0.3867442523911748 1101 10-11 0.16092971417391055 1101 12-13 0.2189527903544075 1101 14-15 0.4097732269866228 1101 16-17 0.3053693810432634 1101 18-19 0.2737678115781321 1101 20-21 0.26749864894621567 1101 22-23 0.22028457293576764 1101 24-25 0.4310884074789598 1101 26-27 0.37322047783073486 1101 28-29 0.28533192856303913 1101 30-31 0.2075631518292127 1101 32-33 0.4178905640383732 1101 34-35 0.3618057629839413 1101 36-37 0.5021105774169428 1101 38-39 0.2512487629416711 1101 40-41 0.15570275231766573 1101 42-43 -0.042984199081374186 1101 44-45 0.30264410012869547 1101 46-47 0.3471203135908638 1101 48-49 0.17995067063539238 1101 50-51 0.24366479657615514 1101 52-53 0.3158372131481215 1101 54-55 0.4548711416457145 1101 56-57 0.3445667448180245 1101 58-59 0.3414164445537189 1101 60-61 0.21598524515612638 1101 62-63 0.45654611656486566 1101 64-65 0.31545013281262513 1101 66-67 -0.030990750045429394 1101 68-69 0.36311036471974134 1101 70-71 0.14902801315330194 1101 72-73 0.09240786365913323 1101 74-75 0.2067296083854515 1101 76-77 0.08961907452525253 1101 78-79 0.15374157103551056 1101 80-81 0.14831824234731883 1101 82-83 0.14127085886823565 1101 84-85 -0.014170107023669942 1101 86-87 -0.08256480100206787 1101 88-89 -0.0924342994074614 1101 90-91 -0.2260918992227161 1101 92-93 0.004627365663846916 1101 94-95 -0.02373817636904363 1101 96-97 -0.03486852957798803 1101 98-99 -0.08146123309668596 1101 100-101 -0.22572462061301124 1101 102-103 -0.20387343334395425 1101 104-105 0.21128966277952443 1101 106-107 -0.05877500941150515 1101 108-109 -0.12622497772003882 1101 110-111 -0.07485430741834875 1101 112-113 0.22179098910732264 1101 114-115 -0.20526397237614802 1101 116-117 -0.03989510560669274 1101 118-119 -0.5327484800118221 1101 120-121 -0.32647501707676696 1101 122-123 -0.2821236501202087 1101 124-125 -0.08178068629573332 1102 1 0.11404909270921593 1102 2 0.19778320782965864 1102 3 0.20574359221750171 1102 4 0.3059477403978903 1102 5 0.2046219125090829 1102 6 0.25680133536927485 1102 7 0.292403747375225 1102 8 0.12614801044016133 1102 9 0.4579005907211595 1102 10-11 0.28952413860681503 1102 12-13 0.29386299351975254 1102 14-15 0.3805943578144877 1102 16-17 0.5708057630471615 1102 18-19 0.3758492103679245 1102 20-21 0.4222680853690335 1102 22-23 0.39702617233983517 1102 24-25 0.5256212598550078 1102 26-27 0.5194541128678338 1102 28-29 0.4998200866054674 1102 30-31 0.6264998246524058 1102 32-33 0.6072419859916565 1102 34-35 0.677968427909093 1102 36-37 0.7982870690137958 1102 38-39 0.9126942878960023 1102 40-41 0.9047792735066622 1102 42-43 0.9273879475731874 1102 44-45 0.8722289938521115 1102 46-47 0.84760501241432 1102 48-49 1.0800478655047243 1102 50-51 1.3262995683595449 1102 52-53 1.3411168078378637 1102 54-55 1.1782905132719534 1102 56-57 1.1473211096584812 1102 58-59 1.1792757594446819 1102 60-61 1.2268705603124026 1102 62-63 1.4625997497652037 1102 64-65 1.4919774443158005 1102 66-67 1.4254947753170732 1102 68-69 1.487083568527943 1102 70-71 1.6912210457352685 1102 72-73 1.7518469905890015 1102 74-75 1.6627036489368834 1102 76-77 1.5427397696343874 1102 78-79 1.6938657049552432 1102 80-81 1.6890826889233033 1102 82-83 1.7067243328832795 1102 84-85 1.8499165163092215 1102 86-87 1.7974582094368152 1102 88-89 1.855593231485713 1102 90-91 1.8417263948415084 1102 92-93 1.9002117593821524 1102 94-95 1.8225089792141773 1102 96-97 1.7670235815660043 1102 98-99 1.799952097651584 1102 100-101 1.640149010750573 1102 102-103 1.7874440801000624 1102 104-105 1.7716321152535315 1102 106-107 1.778503942414531 1102 108-109 1.7575455199380983 1102 110-111 1.7299753337004375 1102 112-113 1.5658378671366897 1102 114-115 1.5100022548302512 1102 116-117 1.7504854084801842 1102 118-119 2.053504951145637 1102 120-121 2.270069335416629 1102 122-123 2.477219557463542 1102 124-125 2.3058329628231355 1103 1 0.05375332154604706 1103 2 0.15677110899103752 1103 3 0.31547092128680276 1103 4 0.22184378690211304 1103 5 0.09473290630901232 1103 6 0.15161566388250236 1103 7 0.08571423360694297 1103 8 0.1235611763478417 1103 9 0.28268177542258144 1103 10-11 0.3193449954466203 1103 12-13 0.04391014934955706 1103 14-15 0.20615453232780823 1103 16-17 0.3183248764685658 1103 18-19 0.22443980222869442 1103 20-21 0.26798033437141555 1103 22-23 0.3406743433060555 1103 24-25 0.14275756592451216 1103 26-27 0.2414466120288239 1103 28-29 0.2114958700659031 1103 30-31 0.1946589835393766 1103 32-33 0.33070550207730776 1103 34-35 0.15829893515883953 1103 36-37 0.06854631584332793 1103 38-39 0.3813301493534027 1103 40-41 0.245572618288044 1103 42-43 0.23450345608450363 1103 44-45 0.11456831495513597 1103 46-47 0.24556102153682957 1103 48-49 0.31646637410668177 1103 50-51 0.16490659739528013 1103 52-53 0.36883708680440463 1103 54-55 0.35912760886478523 1103 56-57 0.30904968315175907 1103 58-59 0.4683116003249914 1103 60-61 0.5846167321164017 1103 62-63 0.6462906916814681 1103 64-65 0.6585575565589217 1103 66-67 0.763444072389003 1103 68-69 0.6816570961024659 1103 70-71 0.8789196207718284 1103 72-73 1.0074193398801938 1103 74-75 0.9967200273201584 1103 76-77 1.030420462200425 1103 78-79 0.8888948680801221 1103 80-81 0.8561049636225455 1103 82-83 1.0696221259904455 1103 84-85 1.0209758199707188 1103 86-87 1.0371445558031205 1103 88-89 0.9585901375733492 1103 90-91 1.0414527685459483 1103 92-93 1.158870672078951 1103 94-95 1.0981611382162875 1103 96-97 1.0782104141885291 1103 98-99 1.249255293714505 1103 100-101 1.2012776641025589 1103 102-103 1.2527288155742262 1103 104-105 1.0159728032632103 1103 106-107 1.0787395990808974 1103 108-109 1.3022921155321505 1103 110-111 1.4576791999885543 1103 112-113 0.9213903742345622 1103 114-115 0.998063868514997 1103 116-117 0.8335874536958912 1103 118-119 0.9898433560782607 1103 120-121 0.9784687073254723 1103 122-123 0.8520520624057184 1103 124-125 1.039806717235571 1104 1 0.12965727980294162 1104 2 0.14115821102519988 1104 3 0.23830325588384227 1104 4 0.295587213586721 1104 5 0.40927369143803105 1104 6 0.41006415762511494 1104 7 0.2630001167751814 1104 8 0.32575431971746127 1104 9 0.41679779189153976 1104 10-11 0.47039876414743986 1104 12-13 0.4601589773630934 1104 14-15 0.5567721182395147 1104 16-17 0.5621592085381906 1104 18-19 0.7146358806600759 1104 20-21 0.6430567682713786 1104 22-23 0.6324887821793226 1104 24-25 0.6322198789162385 1104 26-27 0.5946527593526199 1104 28-29 0.5253844192485673 1104 30-31 0.47821856121660744 1104 32-33 0.3204531682955576 1104 34-35 0.42881379390899355 1104 36-37 0.268354721534088 1104 38-39 0.17402103027957594 1104 40-41 0.12219811256059643 1104 42-43 -0.10718665031148333 1104 44-45 -0.15009680265115577 1104 46-47 -0.12301343527291664 1104 48-49 -0.3072750143405898 1104 50-51 -0.2621448884797317 1104 52-53 -0.2567794799886336 1104 54-55 -0.34836495156895353 1104 56-57 -0.2575191481229524 1104 58-59 -0.21606734875983946 1104 60-61 -0.17760250069106576 1104 62-63 -0.18208972493424014 1104 64-65 0.042663381841219206 1104 66-67 0.17074503851349832 1104 68-69 0.21789695587173696 1104 70-71 0.04206013685352161 1104 72-73 -0.07097126792218234 1104 74-75 -0.21937389465611545 1104 76-77 -0.0323368652627849 1104 78-79 0.0424760510730664 1104 80-81 -0.0664827824946137 1104 82-83 0.066401711005323 1104 84-85 0.026662502127990706 1104 86-87 0.027422163727788273 1104 88-89 0.07097854407939863 1104 90-91 0.09634249990626742 1104 92-93 0.11757598372544109 1104 94-95 0.05965883500810065 1104 96-97 0.047392265723321 1104 98-99 0.1486275166161377 1104 100-101 -0.04117066658106694 1104 102-103 -0.020496898827957466 1104 104-105 0.15235126317108083 1104 106-107 0.05913175594364262 1104 108-109 -0.1885358164722497 1104 110-111 -0.009403267093915701 1104 112-113 0.10678844198557158 1104 114-115 -0.02890201139205928 1104 116-117 -0.20409955457935425 1104 118-119 0.05596377513985473 1104 120-121 0.21145961678697134 1104 122-123 0.40152008246197113 1104 124-125 0.3071623081205743 1105 1 0.21550573496613623 1105 2 0.19291563088449948 1105 3 0.23515782641943161 1105 4 0.1967460910949015 1105 5 0.26821584085926276 1105 6 0.08852840613181456 1105 7 0.296102652000215 1105 8 0.23872632850511621 1105 9 0.23745722140481718 1105 10-11 0.23225465638538623 1105 12-13 0.35325991321720807 1105 14-15 0.1605413023604214 1105 16-17 0.2588996017612999 1105 18-19 0.2596559505436957 1105 20-21 0.21360901771992502 1105 22-23 0.3656093103271374 1105 24-25 0.30504967697344654 1105 26-27 0.3383791158746092 1105 28-29 0.4122411444921994 1105 30-31 0.36463212743611706 1105 32-33 0.46572824898603926 1105 34-35 0.5664929734317212 1105 36-37 0.7213509874472734 1105 38-39 0.7357312020564564 1105 40-41 0.9231319674665528 1105 42-43 0.8452620531984749 1105 44-45 0.7505100697568068 1105 46-47 1.0943332972345203 1105 48-49 1.1153043266741705 1105 50-51 0.9923883284549433 1105 52-53 1.4270459931030643 1105 54-55 1.5820168760233102 1105 56-57 1.3865097560636244 1105 58-59 1.462110345791416 1105 60-61 1.4834485969882891 1105 62-63 1.3432147664102736 1105 64-65 1.371331883341739 1105 66-67 1.226886289621394 1105 68-69 1.2955075401530323 1105 70-71 1.2952805368295301 1105 72-73 1.3996636156902653 1105 74-75 1.435318592965423 1105 76-77 1.416264462659342 1105 78-79 1.35732665428025 1105 80-81 1.467307260088642 1105 82-83 1.5003265894420288 1105 84-85 1.531220709233196 1105 86-87 1.4872313054653716 1105 88-89 1.402091639595163 1105 90-91 1.4857737680519065 1105 92-93 1.580558321783581 1105 94-95 1.5705534386862112 1105 96-97 1.42813583991747 1105 98-99 1.2860823399411885 1105 100-101 1.471997487278415 1105 102-103 1.483326041926457 1105 104-105 1.6427139427393413 1105 106-107 1.4043915093295354 1105 108-109 1.3547938072091021 1105 110-111 1.2357358670688718 1105 112-113 1.4392343465964252 1105 114-115 1.4562671554023332 1105 116-117 1.50393639572464 1105 118-119 1.2833498921560533 1105 120-121 1.0018442877052962 1105 122-123 0.8772129765902825 1105 124-125 0.8518502864046731 1106 1 0.054374419406038044 1106 2 0.061374649248840285 1106 3 0.19228264983922827 1106 4 0.15932611574098843 1106 5 0.1835174548774745 1106 6 0.17086738001297164 1106 7 0.30080644809891766 1106 8 0.21158557529661692 1106 9 0.3320891831733306 1106 10-11 0.19269317641784056 1106 12-13 0.2974455403399361 1106 14-15 0.28217876441446066 1106 16-17 0.39675238646870525 1106 18-19 0.42491999430553307 1106 20-21 0.3176936559935015 1106 22-23 0.40792093306164645 1106 24-25 0.4776173785754452 1106 26-27 0.5084897227093919 1106 28-29 0.3219898746175289 1106 30-31 0.3339146858202966 1106 32-33 0.5556263584507448 1106 34-35 0.5376089603272121 1106 36-37 0.2758203451461725 1106 38-39 0.2242422176556289 1106 40-41 0.24849038771241538 1106 42-43 0.13988800372595023 1106 44-45 0.4162550128002991 1106 46-47 0.15302555175045995 1106 48-49 0.13695982245127425 1106 50-51 0.3382601086839685 1106 52-53 0.12339566699323612 1106 54-55 -0.02937142009042759 1106 56-57 -0.04953815352731539 1106 58-59 -0.019720678223496435 1106 60-61 -7.042688708693845E-4 1106 62-63 -0.00464072335558896 1106 64-65 0.01112329327832029 1106 66-67 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-1.9322524032483628 2216 58-59 -1.7223211893274062 2216 60-61 -1.7850490120591473 2216 62-63 -1.9796038302914845 2216 64-65 -2.074436221532892 2216 66-67 -2.22338098796547 2216 68-69 -2.143982268752584 2216 70-71 -2.1418374107695186 2216 72-73 -1.935903394619011 2216 74-75 -1.817111451217201 2216 76-77 -1.939571097987212 2216 78-79 -1.9490607345813658 2216 80-81 -2.0853727886395212 2216 82-83 -1.747460643053106 2216 84-85 -2.161380065326341 2216 86-87 -2.362282322055126 2216 88-89 -2.185819958797534 2216 90-91 -2.172075465570522 2216 92-93 -2.3358456128334133 2216 94-95 -2.2090971004144997 2216 96-97 -2.4284442797210666 2216 98-99 -2.6639667518604853 2216 100-101 -2.4181496063051036 2216 102-103 -2.573538628340277 2216 104-105 -2.5770800535151395 2216 106-107 -2.806191410359915 2216 108-109 -2.788315158408455 2216 110-111 -2.7297680511841875 2216 112-113 -2.24838724652345 2216 114-115 -2.384597223950017 2216 116-117 -2.3031973703438346 2216 118-119 -2.1677946741087872 2216 120-121 -2.2118686888942776 2216 122-123 -2.0553985254370275 2216 124-125 -2.082271243077681 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 8.0 4 30.0 5 114.0 6 352.0 7 953.0 8 1518.0 9 2103.0 10 3262.0 11 6482.0 12 9338.0 13 9821.0 14 13087.0 15 17348.0 16 7828.0 17 2933.0 18 2903.0 19 3943.0 20 4932.0 21 7444.0 22 7356.0 23 2290.0 24 2796.0 25 3474.0 26 4620.0 27 5478.0 28 6739.0 29 8441.0 30 10490.0 31 14474.0 32 19595.0 33 26430.0 34 36464.0 35 54681.0 36 121019.0 37 50986.0 38 5903.0 39 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.51849027348836 20.734512789614943 12.573048904319101 15.1739480325776 2 19.837273596905256 21.50805747984316 37.4811046053254 21.173564317926186 3 28.392333794460583 23.288748354868957 29.505136172699864 18.813781677970592 4 15.162064320592691 23.551944771847115 27.861382387607343 33.424608519952855 5 14.67286378262424 34.60198952659362 28.371153967486173 22.353992723295967 6 30.12921863304936 36.68160831574255 9.791475691086312 23.397697360121775 7 26.143182114429635 41.51196478053006 12.382317942335266 19.962535162705038 8 34.9007455124189 27.899740563360147 12.430042510606627 24.769471413614326 9 32.30154358493502 14.187441921092578 18.28397589804182 35.22703859593059 10-11 29.568418529746914 24.901712618207657 18.888942616988263 26.640926235057165 12-13 33.40993091500822 26.049204660608936 20.06502735227459 20.47583707210825 14-15 34.46901687389361 19.7851973947011 24.87764009368312 20.868145637722172 16-17 30.68384891284695 20.761448640375406 26.18417899025746 22.370523456520186 18-19 28.82563923590233 20.780790756150584 26.932845014990143 23.460724992956948 20-21 33.89390429016538 17.62550300013035 27.529822599159882 20.950770110544397 22-23 29.701395575701255 20.576752458761348 27.669737577946623 22.05211438759077 24-25 24.458946359267188 22.030039146760405 30.40685720052308 23.10415729344933 26-27 21.97369472254576 21.2996640358586 32.79298049389672 23.93366074769892 28-29 21.659700701782416 18.540679412840642 34.61723634804035 25.182383537336587 30-31 23.01312320507268 19.274733730547506 32.55961366226143 25.152529402118383 32-33 21.22460821703536 19.505998158294194 33.60019846692708 25.669195157743363 34-35 21.000597082704363 20.70562981713291 34.62669716553908 23.66707593462365 36-37 21.82810325325978 20.37839065187135 31.550354675536006 26.243151419332868 38-39 23.397022155132177 17.312560181311312 32.78593744086989 26.50448022268662 40-41 22.091008859529985 17.350403451306224 34.37756230473924 26.18102538442455 42-43 22.908213251031228 17.43639177035022 35.21284736968249 24.442547608936056 44-45 22.440953986788497 18.09486466826169 34.10992628971967 25.35425505523015 46-47 21.621016470232064 17.48790066562107 32.34401214348486 28.547070720662006 48-49 20.810434650979932 18.515135205594078 32.38217077406306 28.29225936936293 50-51 20.99891515959348 19.091929712433195 31.10653721465123 28.80261791332209 52-53 21.10077662799645 19.097290842349143 30.21921765346497 29.582714876189435 54-55 19.2577042590498 20.205888412811206 27.99455897873629 32.541848349402706 56-57 20.181710768092238 21.20789410612094 26.67351349533056 31.936881630456266 58-59 19.817469294391206 21.04317076144864 26.836975397669693 32.30238454649046 60-61 20.12589194484974 22.710377045113383 24.016495460910004 33.147235549126876 62-63 18.62530116935704 23.76199947019422 24.12014397261829 33.49255538783045 64-65 18.1698153668905 23.000613901935473 25.01639875033113 33.813171980842895 66-67 18.491798522430546 23.598327327466226 25.092926251876396 32.816947898226836 68-69 17.93634341506078 23.282125782619847 25.49269835129487 33.288832451024504 70-71 17.371007009414566 22.59432435046232 26.50185221782586 33.53281642229726 72-73 17.421044221963395 20.954133956766167 27.458866467919417 34.16595535335102 74-75 17.024425728377825 20.234481105696254 27.940422078604676 34.80067108732124 76-77 16.637162932096558 20.08237218435559 28.571879086547558 34.70858579700029 78-79 16.710641948003346 20.792879578510068 28.841407265066877 33.655071208419706 80-81 16.69718656311627 21.689765077389485 27.87524755805788 33.737800801436364 82-83 15.80103690559786 20.88496486883102 28.418508722873735 34.89548950269739 84-85 16.040816069093403 20.013833817587027 28.806822721099305 35.13852739222026 86-87 16.475698313451602 20.21997451886487 28.586701033962232 34.717626133721296 88-89 16.404006340850128 20.511472818020124 27.89133094780572 35.19318989332403 90-91 16.46655285653616 19.96011739823314 27.592579355234776 35.98075038999592 92-93 16.484738650172606 19.80559071242058 27.47137577105663 36.23829486635019 94-95 16.209849341737343 19.53816493778987 27.47190137202878 36.78008434844401 96-97 16.031670612177965 19.55193568326024 27.378239278791373 37.03815442577042 98-99 16.45635619767642 20.30196827052051 27.039542012336902 36.20213351946616 100-101 16.68856670717298 21.01258078486942 26.559773444956964 35.73907906300063 102-103 16.32327403152765 21.64960916311711 26.26564714094095 35.76146966441429 104-105 15.861165656811998 23.23934186348671 25.917594177182192 34.9818983025191 106-107 15.442277914959776 24.81858863376191 25.823854109836002 33.915279341442314 108-109 15.042804943172023 25.776312635867853 25.669300277937797 33.51158214302233 110-111 14.789468981430382 26.923687433313887 25.4013255754059 32.88551800984983 112-113 15.09674747264507 27.773835766342792 25.144671819664016 31.984744941348126 114-115 14.852304263728872 28.334769994113195 25.377491380035323 31.435434362122617 116-117 14.39767294195811 28.724386172212384 25.5771389885245 31.300801897305004 118-119 14.330286185234087 29.10008914379904 26.032617379676896 30.53700729128998 120-121 14.676433084862929 29.42308682868577 26.172017487887462 29.728462598563844 122-123 14.55699130003322 29.814521249532206 25.94768246172477 29.68080498870981 124-125 13.960582503241328 31.161967573046866 26.073634146828756 28.803815776883045 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.5 2 2.0 3 2.0 4 2.0 5 4.0 6 5.5 7 8.5 8 14.0 9 21.5 10 29.0 11 27.0 12 27.5 13 33.0 14 34.0 15 42.0 16 63.5 17 78.5 18 87.5 19 113.0 20 150.0 21 215.0 22 322.5 23 457.5 24 633.0 25 890.5 26 1217.0 27 1578.0 28 2100.0 29 2748.0 30 3352.5 31 3944.0 32 4489.0 33 4906.5 34 5253.5 35 5501.0 36 5422.0 37 5239.5 38 5077.0 39 4783.0 40 4586.5 41 4499.0 42 4423.0 43 4422.0 44 4721.0 45 5278.0 46 6015.5 47 7105.0 48 8934.0 49 12103.5 50 17881.5 51 27148.5 52 38137.5 53 45026.0 54 44088.5 55 38966.5 56 32703.5 57 25777.5 58 19198.0 59 13954.5 60 10408.5 61 7964.0 62 6234.0 63 4990.0 64 4037.0 65 3468.5 66 3255.5 67 2995.0 68 2450.5 69 1692.0 70 1125.0 71 913.5 72 684.5 73 540.0 74 390.0 75 165.5 76 86.0 77 69.0 78 85.0 79 113.0 80 83.5 81 30.5 82 8.5 83 8.5 84 4.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20267173486164078 2 2.1024038886062322E-4 3 0.0 4 0.11058644454068783 5 0.03279750066225723 6 0.003784326999491218 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 1.0512019443031161E-4 108-109 0.0 110-111 7.358413610121813E-4 112-113 1.0512019443031161E-4 114-115 0.0012614423331637394 116-117 0.005045769332654958 118-119 0.002102403888606232 120-121 5.25600972151558E-4 122-123 0.002522884666327479 124-125 0.030800216968081304 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 475646.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.565329971861566 #Duplication Level Percentage of deduplicated Percentage of total 1 80.82523379290166 42.48605084376731 2 9.010785234748155 9.473097983402296 3 3.3705443208384547 5.315213232289722 4 1.7810414322758625 3.744841223245506 5 1.0945243891614118 2.8767017839259914 6 0.7262387906946934 2.2904989002739358 7 0.5419248194529621 1.9940519868140518 8 0.41251790761328505 1.7347311946395387 9 0.32579058458470567 1.5412760622378654 >10 1.7955904237996811 16.367903630302447 >50 0.08226382969312329 2.9207413375007305 >100 0.02555769465598917 2.4834907832193265 >500 0.004392728768998139 1.656850033974317 >1k 0.0027953728529988156 2.187512251874618 >5k 7.986779579996616E-4 2.927038752532355 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT 8938 1.8791285956362505 No Hit ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT 5006 1.05246338663628 RNA PCR Primer, Index 39 (95% over 23bp) ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT 3020 0.6349259743590822 RNA PCR Primer, Index 39 (96% over 25bp) ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA 1460 0.3069509677365099 No Hit GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT 1430 0.3006437560706912 No Hit GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT 1298 0.272892024741089 No Hit ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC 1096 0.23042346619124307 No Hit GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA 1072 0.2253776968585881 No Hit CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCT 1045 0.21970120635935128 RNA PCR Primer, Index 39 (96% over 28bp) TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC 947 0.1990976482510102 No Hit TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG 923 0.19405187891835526 RNA PCR Primer, Index 39 (96% over 30bp) TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC 860 0.18080673442013598 No Hit CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTG 803 0.16882303225508047 Illumina PCR Primer Index 11 (96% over 25bp) ACGCAGAGTCGCCGGACAATTCACGCATTAACCGTTCATCCTTTCCCGCA 683 0.14359418559180567 No Hit GGGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGA 662 0.13917913742573257 No Hit GTATCAACGCAGAGTCGCCGGACAATTCACGCATTAACCGTTCATCCTTT 641 0.1347640892596595 No Hit GGTATCAACGCAGAGTCGCCGGACAATTCACGCATTAACCGTTCATCCTT 624 0.13119000264902891 No Hit GTATCAACGCAGAGTGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 609 0.12803639681611956 No Hit TATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCT 579 0.12172918515030086 RNA PCR Primer, Index 39 (95% over 22bp) GGTATCAACGCAGAGTGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 562 0.11815509853967025 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 2.1024038886062325E-4 0.0 0.0 0.0 0.0 3 2.1024038886062325E-4 0.0 0.0 0.0 0.0 4 2.1024038886062325E-4 0.0 0.0 0.0 0.0 5 4.204807777212465E-4 0.0 0.0 0.0 0.0 6 4.204807777212465E-4 0.0 0.0 0.0 0.0 7 6.307211665818697E-4 0.0 0.0 0.0 0.0 8 6.307211665818697E-4 0.0 0.0 0.0 0.0 9 8.40961555442493E-4 0.0 0.0 0.0 0.0 10-11 0.0031536058329093486 0.0 0.0 4.204807777212465E-4 0.0 12-13 0.005991851082527762 0.0 0.0 4.204807777212465E-4 0.0 14-15 0.007043053026830878 0.0 0.0 4.204807777212465E-4 0.0 16-17 0.007358413610121814 0.0 0.0 6.307211665818697E-4 0.0 18-19 0.007989134776703682 0.0 0.0 0.001366562527594051 0.0 20-21 0.009881298276449292 0.0 0.0 0.003994567388351841 0.0 22-23 0.011878581970625213 0.0 0.0 0.01755507246986204 0.0 24-25 0.013140024303788952 0.0 0.0 0.04909113079895552 0.0 26-27 0.014191226248092069 0.0 0.0 0.11973190145612495 0.0 28-29 0.015768029164546744 0.0 0.0 0.36876164206153317 0.0 30-31 0.01734483208100142 0.0 0.0 0.8932062920743578 0.0 32-33 0.018080673442013598 0.0 0.0 1.6315705377528666 0.0 34-35 0.01818579363644391 0.0 0.0 2.684244164778007 0.0 36-37 0.01955235616403796 0.0 0.0 4.130067318972514 0.0 38-39 0.020393317719480455 0.0 0.0 6.049772309658864 0.0 40-41 0.022390601413656376 0.0 0.0 8.144397303877252 0.0 42-43 0.026174928413147593 0.0 0.0 9.827056256123251 0.0 44-45 0.027646611135171955 0.0 0.0 11.33794881067012 0.0 46-47 0.028172212107323513 0.0 0.0 13.1186849043196 0.0 48-49 0.028172212107323513 0.0 0.0 15.11596859849552 0.0 50-51 0.028172212107323513 0.0 0.0 17.0021402471586 0.0 52-53 0.028382452496184138 0.0 0.0 18.705823238290662 0.0 54-55 0.028382452496184138 0.0 0.0 20.35179524268048 0.0 56-57 0.028382452496184138 0.0 0.0 22.070615541810504 0.0 58-59 0.028908053468335693 0.0 0.0 23.78596687452433 0.0 60-61 0.029013173662766005 0.0 0.0 25.562287920007737 0.0 62-63 0.029433654440487254 0.0 0.0 27.247574877114495 0.0 64-65 0.029643894829347876 0.0 0.0 28.894913444031907 0.0 66-67 0.029749015023778188 0.0 0.0 30.678382662736574 0.0 68-69 0.0298541352182085 0.0 0.0 32.538379382986506 0.0 70-71 0.0298541352182085 0.0 0.0 34.58401836660037 0.0 72-73 0.0298541352182085 0.0 0.0 36.635859441685625 0.0 74-75 0.0298541352182085 0.0 0.0 38.40986784289156 0.0 76-77 0.0298541352182085 0.0 0.0 40.27049528430808 0.0 78-79 0.0298541352182085 0.0 0.0 42.14562931255598 0.0 80-81 0.0298541352182085 0.0 0.0 43.9501225701467 0.0 82-83 0.0298541352182085 0.0 0.0 45.86835587811103 0.0 84-85 0.0298541352182085 0.0 0.0 47.96802664166208 0.0 86-87 0.0298541352182085 0.0 0.0 49.67896292620983 0.0 88-89 0.0298541352182085 0.0 0.0 51.17124920634254 0.0 90-91 0.0298541352182085 0.0 0.0 53.039865782535756 0.0 92-93 0.0298541352182085 0.0 0.0 54.73072410994732 0.0 94-95 0.0298541352182085 0.0 0.0 56.21796462074737 0.0 96-97 0.0298541352182085 0.0 0.0 57.66494409708061 0.0 98-99 0.0298541352182085 0.0 0.0 58.921235540717255 0.0 100-101 0.0298541352182085 0.0 0.0 60.094902511531686 0.0 102-103 0.03006437560706912 0.0 0.0 61.21201481774261 0.0 104-105 0.030274615995929746 0.0 0.0 62.27162637760015 0.0 106-107 0.030274615995929746 0.0 0.0 63.235473440331674 0.0 108-109 0.030274615995929746 0.0 0.0 64.14171463651539 0.0 110-111 0.030274615995929746 0.0 0.0 65.03744381325609 0.0 112-113 0.030274615995929746 0.0 0.0 65.93233202844132 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATAAT 30 0.0041801985 59.696095 1 TATTATG 30 0.0042169923 59.56415 2 TAGGGTG 30 0.004234598 59.501522 5 GTATTAG 45 3.11796E-4 53.0632 1 ATGACAG 35 0.0077926703 51.001305 5 TATGGGT 60 2.1775055E-5 49.63679 4 GTTCTAG 50 5.2436546E-4 47.75688 1 GTCTATG 50 5.2436546E-4 47.75688 1 GCCGTAC 50 5.3315196E-4 47.59622 7 GAGATGT 130 1.8189894E-11 45.920074 1 AGTCGCA 150 1.8189894E-12 43.629864 7 TATTAGG 55 8.478257E-4 43.31938 2 CAGATGT 110 9.607902E-9 43.273834 5 CGTACCG 55 8.526703E-4 43.269283 9 TATATGG 140 4.0017767E-11 42.545822 2 GCTTAAC 35 5.4538195E-5 42.49662 20-21 CGGAGAT 90 5.0340077E-6 39.7974 1 GTTACAG 75 8.129612E-5 39.709435 3 AGTTAGC 60 0.0013008283 39.709435 2 TAGGAGG 60 0.0013008283 39.709435 4 >>END_MODULE