FastQCFastQC Report
Thu 26 May 2016
SRR935989_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935989_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419682
Sequences flagged as poor quality0
Sequence length125
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT10320.24590046749681901No Hit
ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA10300.24542391620322054No Hit
GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT9680.23065082610166746No Hit
GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA7600.18108949156742488No Hit
ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC7250.17274984392945134No Hit
TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC6120.14582469584113686No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGACTA250.002057250171.388499
TTAGGGC401.7710477E-459.4974984
TTATGCC300.004235385559.4974984
TAACAAC2150.055.4191471
TTCACTA350.007797792650.991788
GTCTTAG653.4900688E-545.8273771
TCTTAGG653.517221E-545.7673072
TCGTAGG705.4473854E-542.498212
GTCATAG1309.0221874E-1041.244641
CCATCCG300.001308581139.66499772-73
GTAGTAG957.3962074E-637.6266861
AGGTATA401.19833145E-437.18150710-11
GTACTAG650.001925457336.6619031
AGTAGAG2950.036.303562
TATAAGG1001.0608885E-535.6984982
AGTAAGA1902.910383E-1134.445922
CGCAGAA3300.034.2561342
ATCCGAG700.002769682634.0431941
TTAGGGA700.002787553533.998574
GTAGGGT1051.4818752E-533.998574