##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935989_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 419682 Sequences flagged as poor quality 0 Sequence length 125 %GC 50 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.385601479215214 31.0 30.0 34.0 12.0 34.0 2 28.222201571666165 31.0 30.0 34.0 7.0 34.0 3 28.25818357708932 31.0 30.0 34.0 7.0 34.0 4 31.318846174007938 37.0 33.0 37.0 7.0 37.0 5 31.553271286354907 37.0 33.0 37.0 10.0 37.0 6 31.706172768905983 37.0 33.0 37.0 8.0 37.0 7 31.709444293536535 37.0 33.0 37.0 8.0 37.0 8 31.403205284000745 37.0 33.0 37.0 8.0 37.0 9 32.52817609523401 39.0 34.0 39.0 7.0 39.0 10-11 32.639506817066255 39.0 34.0 39.0 7.0 39.0 12-13 32.64581873894997 39.0 33.5 39.0 7.0 39.0 14-15 33.66093852011761 40.0 33.0 41.0 7.5 41.0 16-17 33.82096801864269 39.5 33.0 41.0 8.0 41.0 18-19 34.06065187451451 40.0 33.5 41.0 9.5 41.0 20-21 34.25699458161179 40.0 34.0 41.0 11.0 41.0 22-23 34.03509919415176 40.0 33.0 41.0 8.0 41.0 24-25 33.75748304668773 40.0 32.0 41.0 2.0 41.0 26-27 33.548110235845236 39.0 32.0 41.0 2.0 41.0 28-29 33.297406131308946 39.0 32.5 41.0 2.0 41.0 30-31 33.01651011956672 39.0 32.0 41.0 2.0 41.0 32-33 32.64744258748291 39.0 32.0 41.0 2.0 41.0 34-35 32.290934564741875 39.0 32.0 41.0 2.0 41.0 36-37 32.07146720612273 39.0 32.0 41.0 2.0 41.0 38-39 31.887821016865153 39.0 32.0 41.0 2.0 41.0 40-41 31.62705572314276 38.5 31.0 41.0 2.0 41.0 42-43 31.332969962971966 38.0 31.0 41.0 2.0 41.0 44-45 30.975206227572308 37.5 31.0 40.5 2.0 41.0 46-47 30.586919381817662 37.0 30.0 40.0 2.0 41.0 48-49 30.183619978936434 36.0 30.0 40.0 2.0 41.0 50-51 29.781390433709333 35.5 30.0 39.0 2.0 41.0 52-53 29.408703494550636 35.0 29.0 39.0 2.0 41.0 54-55 29.019352748033036 35.0 29.0 37.5 2.0 40.5 56-57 28.662344346433727 35.0 29.0 37.0 2.0 40.0 58-59 28.3151862600731 35.0 28.0 36.5 2.0 39.0 60-61 28.00302014382318 35.0 27.0 36.0 2.0 39.0 62-63 27.70838515828651 35.0 27.0 35.0 2.0 37.5 64-65 27.49657836171196 35.0 26.0 35.0 2.0 37.0 66-67 27.376890121568234 35.0 26.5 35.0 2.0 36.5 68-69 27.29065339947865 35.0 27.0 35.0 2.0 36.0 70-71 27.122957382017812 35.0 26.0 35.0 2.0 35.5 72-73 26.982935889554472 35.0 26.0 35.0 2.0 35.0 74-75 26.835056066259693 35.0 25.5 35.0 2.0 35.0 76-77 26.690502570994227 35.0 25.0 35.0 2.0 35.0 78-79 26.537248440485893 34.5 24.5 35.0 2.0 35.0 80-81 26.372191325813354 34.0 24.0 35.0 2.0 35.0 82-83 26.232809603461668 34.0 23.5 35.0 2.0 35.0 84-85 26.114962042689463 34.0 20.5 35.0 2.0 35.0 86-87 25.980283881605594 34.0 19.5 35.0 2.0 35.0 88-89 25.758387302767332 34.0 18.5 35.0 2.0 35.0 90-91 25.49815098098084 34.0 16.0 35.0 2.0 35.0 92-93 25.213186412569517 34.0 9.0 35.0 2.0 35.0 94-95 24.9946661996464 33.5 6.0 35.0 2.0 35.0 96-97 24.786169051805892 33.0 2.0 35.0 2.0 35.0 98-99 24.52381684227582 33.0 2.0 35.0 2.0 35.0 100-101 24.298777407656274 33.0 2.0 35.0 2.0 35.0 102-103 23.6325205751021 31.5 2.0 34.5 2.0 35.0 104-105 24.066053583427454 33.0 2.0 35.0 2.0 35.0 106-107 24.06833030723262 33.0 2.0 35.0 2.0 35.0 108-109 23.867279273354587 33.0 2.0 35.0 2.0 35.0 110-111 23.523938362855684 33.0 2.0 35.0 2.0 35.0 112-113 23.06514694459138 32.5 2.0 35.0 2.0 35.0 114-115 22.503104731677794 32.0 2.0 35.0 2.0 35.0 116-117 21.883200852073713 31.5 2.0 35.0 2.0 35.0 118-119 21.086511930461633 31.0 2.0 35.0 2.0 35.0 120-121 20.282460291363464 29.5 2.0 35.0 2.0 35.0 122-123 19.47074451608599 29.0 2.0 35.0 2.0 35.0 124-125 17.090229030551704 21.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4086758566709676 1101 2 0.42500683549027585 1101 3 0.24127478856905782 1101 4 0.272618244998867 1101 5 0.21252259295293285 1101 6 0.4688216283068911 1101 7 0.09119995783700219 1101 8 0.06296255902703862 1101 9 0.46954467873751327 1101 10-11 0.26525910100009753 1101 12-13 0.002928583366518467 1101 14-15 0.21794010655128915 1101 16-17 0.1965429155404479 1101 18-19 0.23632021351989607 1101 20-21 0.2726541799202451 1101 22-23 0.22667284014888622 1101 24-25 0.2168001780549602 1101 26-27 0.16229471791235284 1101 28-29 0.3674394654780002 1101 30-31 0.5254982381290247 1101 32-33 0.6325146653040008 1101 34-35 0.31278169657737465 1101 36-37 0.289894717319207 1101 38-39 0.4934959058882029 1101 40-41 0.6496939286364736 1101 42-43 0.6903048955691666 1101 44-45 0.8597806618708859 1101 46-47 0.7861555607621789 1101 48-49 0.6266746700659596 1101 50-51 0.7945809994109254 1101 52-53 0.6591667484817911 1101 54-55 0.3577639892226543 1101 56-57 0.3580555176297082 1101 58-59 0.4309664039744945 1101 60-61 0.4279257309451978 1101 62-63 0.3067987082363217 1101 64-65 0.20113212783074275 1101 66-67 0.21617091380841558 1101 68-69 0.23960068216474184 1101 70-71 0.1924964353088896 1101 72-73 0.1885967108283566 1101 74-75 0.370495440656633 1101 76-77 0.3715294115405001 1101 78-79 0.5829125859114761 1101 80-81 0.4856308276571184 1101 82-83 0.43918977405510873 1101 84-85 0.35903082119055796 1101 86-87 0.3051655291224584 1101 88-89 0.3712186455095363 1101 90-91 0.21777470414987832 1101 92-93 0.0020506934582016356 1101 94-95 0.23849808449666554 1101 96-97 0.04071354224381096 1101 98-99 -0.2675670470642224 1101 100-101 -0.3622165652675058 1101 102-103 -0.13474348979516648 1101 104-105 -0.22560450320004932 1101 106-107 0.16709588445434065 1101 108-109 0.2504156799602164 1101 110-111 0.11701527143913637 1101 112-113 -0.16229707467629595 1101 114-115 -0.48686531234908514 1101 116-117 -0.14982223349204205 1101 118-119 -0.005165271637615376 1101 120-121 -0.5889146454863017 1101 122-123 -0.7275105415306236 1101 124-125 -0.663170074714639 1102 1 -0.8119176410095648 1102 2 -0.8861305173877518 1102 3 -0.6967143980879804 1102 4 -0.8985930185747435 1102 5 -0.9508454185500668 1102 6 -1.0777726891577615 1102 7 -0.9148605890761203 1102 8 -0.9201570448740917 1102 9 -1.0779540037522892 1102 10-11 -0.9273722759119138 1102 12-13 -0.7471787693163279 1102 14-15 -0.9409434906093921 1102 16-17 -0.9181715588543042 1102 18-19 -0.8836659282246799 1102 20-21 -0.9055333814822291 1102 22-23 -0.9593124297617877 1102 24-25 -0.8065295117933857 1102 26-27 -1.013233424756116 1102 28-29 -1.1066901645130827 1102 30-31 -1.2665563620960896 1102 32-33 -1.206662883020968 1102 34-35 -1.2219664149913427 1102 36-37 -1.3262251012872923 1102 38-39 -1.350001774643225 1102 40-41 -1.219789607365712 1102 42-43 -1.2820338964854265 1102 44-45 -1.5787778193163717 1102 46-47 -1.5290091442781915 1102 48-49 -1.3261552117159638 1102 50-51 -1.27370018654344 1102 52-53 -1.2291620630264184 1102 54-55 -1.1943020619552982 1102 56-57 -1.1458986395216044 1102 58-59 -1.1405987981413297 1102 60-61 -1.0918027643605583 1102 62-63 -1.013097647628058 1102 64-65 -1.0323745963509339 1102 66-67 -1.246171066295556 1102 68-69 -1.2971822507105522 1102 70-71 -1.187367779812874 1102 72-73 -1.208600082904848 1102 74-75 -1.0021631322683682 1102 76-77 -0.9450569032696166 1102 78-79 -0.8778435022868862 1102 80-81 -0.9331926520011748 1102 82-83 -0.857986850386169 1102 84-85 -1.0173720831874817 1102 86-87 -1.0201374982231997 1102 88-89 -1.157724288406751 1102 90-91 -0.8814565855787464 1102 92-93 -0.522165469301477 1102 94-95 -0.7247157295164364 1102 96-97 -0.909086066081553 1102 98-99 -1.0377558576615584 1102 100-101 -0.741317925718775 1102 102-103 -0.6930961791425254 1102 104-105 -0.7278849344325238 1102 106-107 -0.9185130004440651 1102 108-109 -0.7533351577160197 1102 110-111 -0.9883074697628196 1102 112-113 -0.9939176447515692 1102 114-115 -0.7558602309887625 1102 116-117 -1.118972976177421 1102 118-119 -1.759028341664095 1102 120-121 -1.5352887272955513 1102 122-123 -1.3843576908289315 1102 124-125 -1.019872135235527 1103 1 0.45895890695401675 1103 2 0.43349834398178544 1103 3 0.4310849783792463 1103 4 0.5926315650121836 1103 5 0.6619897924201332 1103 6 0.5313424408277072 1103 7 0.5517394183764637 1103 8 0.35924960531408345 1103 9 0.7904737496665817 1103 10-11 0.6591568698978705 1103 12-13 0.6377937182316487 1103 14-15 0.7796700432812287 1103 16-17 0.8146331586306914 1103 18-19 0.5172892444889285 1103 20-21 0.5575359648020282 1103 22-23 0.7104806989567507 1103 24-25 0.5848071710619536 1103 26-27 0.5234752040928328 1103 28-29 0.5107128587513969 1103 30-31 0.8577076953384832 1103 32-33 0.9945692773586146 1103 34-35 0.9714979802936554 1103 36-37 0.9904025428270344 1103 38-39 1.0300426924349892 1103 40-41 1.237980641923187 1103 42-43 1.0163954716597452 1103 44-45 0.995103672193892 1103 46-47 0.9838745084811293 1103 48-49 0.8056623490536374 1103 50-51 0.9458047756347021 1103 52-53 1.0205553598704036 1103 54-55 1.164207545666212 1103 56-57 1.0181870527612418 1103 58-59 0.9469504199585117 1103 60-61 1.04168696970644 1103 62-63 0.6761793276169428 1103 64-65 0.8823259340245535 1103 66-67 1.1220983197358159 1103 68-69 1.2323579249219883 1103 70-71 1.3572483500608037 1103 72-73 1.2623146595463055 1103 74-75 1.299025244186435 1103 76-77 1.3742350392461304 1103 78-79 1.3936851466840352 1103 80-81 1.3471859392122276 1103 82-83 1.4184605033258393 1103 84-85 1.3582815704413065 1103 86-87 1.5710246699815968 1103 88-89 1.3583981326890253 1103 90-91 1.537288523663701 1103 92-93 1.6106504270579336 1103 94-95 1.7347934557920368 1103 96-97 1.4955503720806398 1103 98-99 1.866840212343039 1103 100-101 1.6562649532140128 1103 102-103 1.5971912421395658 1103 104-105 1.5367997592042109 1103 106-107 1.3581548255132816 1103 108-109 1.212786642331178 1103 110-111 1.2611211655450312 1103 112-113 1.3336652962860782 1103 114-115 1.395585570101801 1103 116-117 1.1602427015728942 1103 118-119 1.5381247716524307 1103 120-121 1.331331774760116 1103 122-123 1.224162785142699 1103 124-125 0.9679071813626194 1104 1 0.16578015588052963 1104 2 0.15379033269482534 1104 3 0.39523661621509376 1104 4 0.49280332307868235 1104 5 0.31753512428125674 1104 6 0.3623631744800164 1104 7 0.454781989840086 1104 8 0.5131264302435419 1104 9 0.30960637143078884 1104 10-11 0.39221109637314555 1104 12-13 0.212066813557378 1104 14-15 0.43524297911732646 1104 16-17 0.40944054422755727 1104 18-19 0.16709908377244886 1104 20-21 0.1362072934733618 1104 22-23 0.18637016037252607 1104 24-25 0.08731299856778207 1104 26-27 0.53822492805309 1104 28-29 0.6087253725533799 1104 30-31 0.5498282940380292 1104 32-33 0.31053664332598174 1104 34-35 0.4395601812505987 1104 36-37 0.6533610755224046 1104 38-39 0.5776196768540842 1104 40-41 0.49949149948667326 1104 42-43 0.5137577584957178 1104 44-45 0.1603686703536269 1104 46-47 0.5275378626707976 1104 48-49 0.591933972167368 1104 50-51 0.5495742517725972 1104 52-53 0.5496622179246309 1104 54-55 0.797700368632718 1104 56-57 0.7771031366773258 1104 58-59 0.8718860106835784 1104 60-61 0.7036149540028447 1104 62-63 0.7936986503994241 1104 64-65 0.6349667142969118 1104 66-67 0.7550083921195743 1104 68-69 0.6609617772100478 1104 70-71 0.7558374802288839 1104 72-73 0.7074900981427987 1104 74-75 0.8104992964499651 1104 76-77 0.9292988350993987 1104 78-79 0.9623515679820365 1104 80-81 0.8563075193864442 1104 82-83 0.8413104603863211 1104 84-85 0.8788495989040683 1104 86-87 0.8830814728278717 1104 88-89 0.8005708722301854 1104 90-91 0.8879694217130201 1104 92-93 0.8105430782663738 1104 94-95 0.8088855917262805 1104 96-97 0.7031291967647313 1104 98-99 0.4844668839170758 1104 100-101 0.7459962745242805 1104 102-103 0.8308435547392499 1104 104-105 0.7577481090999285 1104 106-107 0.8038608738509083 1104 108-109 0.7081369061024958 1104 110-111 0.41075924676205844 1104 112-113 0.42755254938385434 1104 114-115 0.3298881096674968 1104 116-117 0.1837520869243825 1104 118-119 0.1845975970726208 1104 120-121 -0.030133076178415763 1104 122-123 0.08932963583586684 1104 124-125 0.03530688692021755 1105 1 -0.06953412319464292 1105 2 -0.15269857168532752 1105 3 -0.21802584457263663 1105 4 0.07182621142537471 1105 5 -0.16180727144315554 1105 6 -0.3410236037237695 1105 7 -0.29883715736567495 1105 8 -0.1545858969320122 1105 9 -0.20536981074651095 1105 10-11 -0.15368178151693712 1105 12-13 -0.10536957542833392 1105 14-15 -0.08096766338297101 1105 16-17 -0.07633266362652336 1105 18-19 -0.09490567979209175 1105 20-21 -0.16925628268558057 1105 22-23 -0.19698931447353374 1105 24-25 0.003096663026937563 1105 26-27 -0.14046102524074655 1105 28-29 -0.09835026355675325 1105 30-31 -0.03987485456194406 1105 32-33 0.04403553957322259 1105 34-35 0.04893627048028293 1105 36-37 -0.08440179191107688 1105 38-39 0.01695974888846763 1105 40-41 -0.035991247114928626 1105 42-43 0.00407087572587983 1105 44-45 -0.01767245895971925 1105 46-47 0.1300503525046537 1105 48-49 0.1950709536940316 1105 50-51 0.22084182467837365 1105 52-53 0.12576306607148524 1105 54-55 0.18166690749643166 1105 56-57 0.1592223419753438 1105 58-59 0.011664679209193451 1105 60-61 -0.1500070978154433 1105 62-63 -0.096628989893361 1105 64-65 -0.18817723829981148 1105 66-67 -0.031278932151366234 1105 68-69 -0.07371348710968917 1105 70-71 -0.06432924453003608 1105 72-73 -0.1135131550232309 1105 74-75 -0.04475514760720145 1105 76-77 -0.05239048754496167 1105 78-79 0.019947129734095626 1105 80-81 -0.046338582623551616 1105 82-83 -0.19477063639536496 1105 84-85 -0.059897932175282165 1105 86-87 0.1654649978656373 1105 88-89 -0.042455153098035936 1105 90-91 0.09902728159450902 1105 92-93 0.09571503132783477 1105 94-95 0.03575325757965686 1105 96-97 0.02101580069176734 1105 98-99 0.0013513965230949054 1105 100-101 -0.17875102418607014 1105 102-103 -0.23626751764369658 1105 104-105 -0.1536786365721987 1105 106-107 -0.1448901427634759 1105 108-109 -0.2070716450767769 1105 110-111 -0.33109621971871306 1105 112-113 0.003778295869274473 1105 114-115 0.2989975554674267 1105 116-117 0.03291555851727068 1105 118-119 -0.1677231883346053 1105 120-121 -0.3068148468302141 1105 122-123 -0.11825721207862117 1105 124-125 0.038792421949843 1106 1 -0.09800026290837849 1106 2 -0.04971843923499719 1106 3 -0.21180922419237547 1106 4 -0.5950291613582195 1106 5 0.007376663613456458 1106 6 -0.16279245653299768 1106 7 -0.21216764230479157 1106 8 0.01725772622543076 1106 9 0.20219267590164236 1106 10-11 0.11612167928203121 1106 12-13 0.04334864717367992 1106 14-15 0.03977042676870468 1106 16-17 0.02920568288063663 1106 18-19 -0.041418177121720134 1106 20-21 0.15072342250562087 1106 22-23 0.1973067761054068 1106 24-25 0.2706463319011192 1106 26-27 0.2996425850989297 1106 28-29 0.09569481228173515 1106 30-31 0.23156287241946671 1106 32-33 0.4245151379496406 1106 34-35 0.32215780434057706 1106 36-37 0.1415755633548912 1106 38-39 0.0964085846073317 1106 40-41 0.044483010522327504 1106 42-43 -0.19072724433393873 1106 44-45 0.005869519250062183 1106 46-47 0.09304437087679673 1106 48-49 0.07228201970556469 1106 50-51 -0.16236689140793104 1106 52-53 -0.10715725010027555 1106 54-55 -0.19011796753027 1106 56-57 -0.10918718590331622 1106 58-59 0.025790934282898803 1106 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-0.06987795017251486 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 32890.0 3 3271.0 4 1861.0 5 3877.0 6 10846.0 7 9100.0 8 5501.0 9 5610.0 10 4096.0 11 2562.0 12 2071.0 13 1619.0 14 1610.0 15 1682.0 16 1594.0 17 1422.0 18 1601.0 19 1788.0 20 2176.0 21 2519.0 22 2903.0 23 3288.0 24 3840.0 25 4177.0 26 4592.0 27 5074.0 28 6015.0 29 7398.0 30 9562.0 31 12769.0 32 17439.0 33 24080.0 34 35341.0 35 55732.0 36 98132.0 37 29860.0 38 1782.0 39 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 56.28701648889207 18.42723172739638 10.825637721621687 14.460114062089865 2 19.71884018597823 25.89039104521006 33.314268419685476 21.07650034912623 3 30.726741553756188 24.83545968821934 24.4687290005576 19.969069757466865 4 17.103836465847376 23.419623431062053 27.84184199928032 31.634698103810255 5 16.127079893154907 35.44021178393654 24.893905493086287 23.538802829822266 6 31.084249503195277 37.018027935436834 8.644640465876545 23.253082095491347 7 28.128916655944263 42.106404372834675 11.743415252500702 18.021263718720366 8 34.2564131890336 30.716590180184046 10.205822503705186 24.821174127077168 9 35.66247778079594 19.029884531621562 14.113781386859575 31.193856300722928 10-11 29.844263037252016 28.89401975781663 14.12116793191035 27.140549273021 12-13 32.28337169571247 30.381455482960906 16.510715255836562 20.82445756549006 14-15 35.50616895649563 24.10015201986266 20.096644602341772 20.297034421299937 16-17 29.595741537640404 25.266749586591754 21.419193579900973 23.71831529586687 18-19 28.51325527423144 25.717090559042322 21.163642948708784 24.60601121801745 20-21 37.7501298602275 23.34422253039206 17.53434743448611 21.371300174894326 22-23 33.73399383342626 26.446690589541603 16.567067446304584 23.25224813072755 24-25 26.849376432632326 28.747718510681896 18.375937018981038 26.02696803770474 26-27 23.843608857732765 28.7512941453994 20.924588703019097 26.480508293848743 28-29 25.34073158846034 27.591424728075996 22.503118681467942 24.56472500199572 30-31 26.400465114062555 28.626317068637686 19.793915393083335 25.17930242421643 32-33 23.66494155098384 28.892947517406036 20.942999699772685 26.499111231837443 34-35 22.589365281332054 29.586687063062033 21.135407284563072 26.688540371042833 36-37 23.351847351089635 30.751378424616732 19.3321371895864 26.56463703470723 38-39 23.70318479229512 28.209692100209203 20.790741561467968 27.29638154602771 40-41 22.108405888267786 28.3382418116574 22.316539665746923 27.236812634327894 42-43 22.004398568439914 28.570203153816458 23.81326814111637 25.612130136627254 44-45 22.26376160998089 28.228754151953144 24.184025047535993 25.323459190529974 46-47 20.246758259825295 28.22911156542334 23.78658126867485 27.737548906076505 48-49 19.513941508094224 28.59665175061118 24.741232647576023 27.14817409371858 50-51 20.369589355750307 28.374459709970886 23.7102139238757 27.545737010403116 52-53 20.492777865145513 27.181413546447068 23.181718539274975 29.144090049132437 54-55 19.59126195548058 26.704981390671982 22.997531464299158 30.706225189548274 56-57 20.25617167386065 25.932765360233223 23.89990457003499 29.911158395871134 58-59 22.048122149627574 24.419203110926844 23.482184129888818 30.050490609556757 60-61 22.037518883345008 24.2759994472005 23.01933368598129 30.667147983473203 62-63 22.391119943195086 23.689245666957362 23.247482617791565 30.672151772055983 64-65 22.687371626548224 23.106268198649428 23.504315254221133 30.702044920581212 66-67 23.830900538979513 24.059764297730187 22.628680763053932 29.480654400236368 68-69 23.702997664776248 24.073893151598913 22.92558261449745 29.297526569127392 70-71 23.40244255561527 23.468576732923978 23.93246925952362 29.196511451937134 72-73 24.671000116755344 22.137409127408674 24.687798589881364 28.503792165954618 74-75 25.348147265578458 21.282735613010843 25.28130585011319 28.087811271297507 76-77 25.172273292635854 21.110507479472552 25.70815522228735 28.009064005604245 78-79 25.66768212409708 21.698462755507478 26.345818654473234 26.28803646592221 80-81 26.718968731942983 22.248869667780617 25.527220306068347 25.50494129420805 82-83 25.97073498506012 21.853212670545794 26.040668887395697 26.135383456998397 84-85 25.826624531098812 21.608539602190667 26.636089781171503 25.928746085539018 86-87 26.262289300742957 21.634649751950324 26.297078208323605 25.80598273898311 88-89 26.536417030799242 21.487376803632944 25.881157137256633 26.095049028311184 90-91 27.389579955275607 21.47735842537734 25.439053186761868 25.694008432585186 92-93 27.50112601609548 22.375071790973283 25.024009761189266 25.099792431741974 94-95 26.420719254388995 23.317740194314503 24.597084567558486 25.664455983738016 96-97 25.83897375165162 23.88717654221408 23.895039966449392 26.378809739684918 98-99 25.24474383062071 25.32564005113499 23.634682675937366 25.794933442306938 100-101 24.866952043166275 27.162036744706374 23.297897562306474 24.673113649820873 102-103 24.15578938339028 28.825992060655448 23.427380731124995 23.590837824829276 104-105 23.259745520396493 30.662052039649257 23.19695958825772 22.88124285169653 106-107 22.531506222612997 31.81169417578673 22.704138162738666 22.952661438861607 108-109 22.416019748285606 33.28829923608828 22.494174160435758 21.801506855190357 110-111 22.24136369918176 35.50211827050004 21.397033944748642 20.859484085569548 112-113 22.154432316127327 36.50087328648801 20.795655727353523 20.54903867003114 114-115 21.99108327912291 36.997600444168036 21.064621206164023 19.946695070545033 116-117 21.868938863234543 37.3750839921655 20.713540251905012 20.042436892694944 118-119 22.294099272989992 37.648579242660155 20.585661521880642 19.47165996246921 120-121 22.83875456601148 36.938288255015166 20.54210738258232 19.680849796391033 122-123 23.485427049464512 37.122153529783915 20.330287646441384 19.062131774310192 124-125 24.361556932989597 38.53620500867497 18.969702686792434 18.132535371543 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8.0 1 10.5 2 13.0 3 14.0 4 13.0 5 7.5 6 11.5 7 14.5 8 14.0 9 20.0 10 17.0 11 15.5 12 22.5 13 25.5 14 37.0 15 56.5 16 69.5 17 94.0 18 129.0 19 175.0 20 248.5 21 346.5 22 477.0 23 656.0 24 870.5 25 1131.5 26 1419.0 27 1706.0 28 2034.5 29 2382.5 30 2659.5 31 2968.0 32 3275.5 33 3432.5 34 3574.0 35 3699.5 36 3763.0 37 3686.5 38 3535.5 39 3348.0 40 3087.5 41 2790.5 42 2524.0 43 2341.5 44 2244.5 45 2441.5 46 2892.0 47 3712.0 48 4942.0 49 7408.0 50 12371.0 51 20231.5 52 30466.0 53 39580.0 54 43375.5 55 41642.0 56 36265.0 57 28357.0 58 20605.5 59 14853.0 60 10838.5 61 8140.0 62 6392.5 63 5119.0 64 4101.5 65 3580.0 66 3149.5 67 2566.0 68 1975.5 69 1517.5 70 1119.5 71 808.5 72 594.5 73 466.0 74 346.0 75 215.5 76 139.0 77 100.5 78 81.5 79 70.0 80 55.5 81 43.0 82 30.5 83 25.0 84 25.0 85 20.5 86 11.5 87 7.5 88 9.0 89 6.5 90 3.5 91 3.5 92 3.5 93 1.5 94 0.5 95 0.0 96 1.5 97 2.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14582469584113686 2 0.015011365748352325 3 0.006671718110378811 4 0.012152057986761404 5 0.0021444808211931892 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 8.339647637973514E-4 28-29 0.00798223416777465 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 5.956891169981081E-4 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0019062051743939461 66-67 0.0 68-69 0.005242064229583352 70-71 0.01918118956733908 72-73 2.3827564679924327E-4 74-75 0.0076248206975757845 76-77 0.0 78-79 5.956891169981081E-4 80-81 0.0010722404105965946 82-83 0.0 84-85 0.02001515433113643 86-87 0.0019062051743939461 88-89 0.01846636262694135 90-91 1.1913782339962163E-4 92-93 0.014534814454753839 94-95 0.01262860928035989 96-97 0.0041698238189867565 98-99 0.0020253429977935677 100-101 5.956891169981081E-4 102-103 0.0 104-105 4.7655129359848653E-4 106-107 7.148269403977297E-4 108-109 0.0 110-111 0.0 112-113 7.148269403977297E-4 114-115 0.005003788582784108 116-117 0.0 118-119 0.06981476451217827 120-121 0.0011913782339962162 122-123 0.0041698238189867565 124-125 0.2260044509890822 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 419682.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.58376493785785 #Duplication Level Percentage of deduplicated Percentage of total 1 85.38298226839616 56.85120421052174 2 7.450258624413506 9.92132537948394 3 2.5926348238324164 5.1788216303928625 4 1.2756554105155722 3.3975175998190164 5 0.7936949157358155 2.642359785086321 6 0.5420450215304214 2.165483897959061 7 0.38672449467097997 1.8024700994219072 8 0.2822062150678657 1.5032281830465062 9 0.21128496614508155 1.2661333668636627 >10 1.0442641018398449 11.471527819388331 >50 0.02430787602652252 1.1370032069402212 >100 0.011796469237148876 1.4426159582180953 >500 0.0014298750590483486 0.7295195366999607 >1k 7.149375295241743E-4 0.49078932615834225 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT 1032 0.24590046749681901 No Hit ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA 1030 0.24542391620322054 No Hit GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT 968 0.23065082610166746 No Hit GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA 760 0.18108949156742488 No Hit ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC 725 0.17274984392945134 No Hit TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC 612 0.14582469584113686 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 4.765512935984865E-4 0.0 0.0 0.0 0.0 3 4.765512935984865E-4 0.0 0.0 0.0 0.0 4 4.765512935984865E-4 0.0 0.0 0.0 0.0 5 0.0014296538807954595 0.0 0.0 0.0 0.0 6 0.0014296538807954595 0.0 0.0 0.0 0.0 7 0.0014296538807954595 0.0 0.0 0.0 0.0 8 0.0014296538807954595 0.0 0.0 0.0 0.0 9 0.0014296538807954595 0.0 0.0 0.0 0.0 10-11 0.0023827564679924323 0.0 0.0 2.3827564679924324E-4 0.0 12-13 0.005003788582784108 0.0 0.0 5.956891169981081E-4 0.0 14-15 0.005956891169981081 0.0 0.0 7.148269403977297E-4 0.0 16-17 0.006195166816780324 0.0 0.0 0.0020253429977935677 0.0 18-19 0.006790855933778432 0.0 0.0 0.002621032114791676 0.0 20-21 0.0076248206975757845 0.0 0.0 0.006433442463579567 0.0 22-23 0.008339647637973513 0.0 0.0 0.02204049732893 0.0 24-25 0.008577923284772757 0.0 0.0 0.04634461330245281 0.0 26-27 0.008697061108172378 0.0 0.0 0.11925696122302124 0.0 28-29 0.009411888048570108 0.0 0.0 0.4022092917971226 0.0 30-31 0.00953102587196973 0.0 0.0 0.9978984087952307 0.0 32-33 0.00953102587196973 0.0 0.0 1.844134368402743 0.0 34-35 0.010245852812367458 0.0 0.0 3.0674415390700576 0.0 36-37 0.01191378233996216 0.0 0.0 4.723338146501399 0.0 38-39 0.012390333633560648 0.0 0.0 6.839464165725478 0.0 40-41 0.013105160573958378 0.0 0.0 9.224603390185903 0.0 42-43 0.013581711867556863 0.0 0.0 11.107457551193523 0.0 44-45 0.013819987514356107 0.0 0.0 12.796355335706558 0.0 46-47 0.014058263161155351 0.0 0.0 14.769158553380894 0.0 48-49 0.014058263161155351 0.0 0.0 16.927340224265038 0.0 50-51 0.014058263161155351 0.0 0.0 18.978416991912923 0.0 52-53 0.014058263161155351 0.0 0.0 20.80932706191831 0.0 54-55 0.014296538807954595 0.0 0.0 22.58197873628128 0.0 56-57 0.014296538807954595 0.0 0.0 24.425279139920228 0.0 58-59 0.01465395227815346 0.0 0.0 26.182085483771047 0.0 60-61 0.014773090101553081 0.0 0.0 28.00525159525546 0.0 62-63 0.014773090101553081 0.0 0.0 29.755981910112894 0.0 64-65 0.014773090101553081 0.0 0.0 31.431297982758373 0.0 66-67 0.014773090101553081 0.0 0.0 33.241954622785826 0.0 68-69 0.014773090101553081 0.0 0.0 35.121472924738256 0.0 70-71 0.014773090101553081 0.0 0.0 37.1214395661477 0.0 72-73 0.014773090101553081 0.0 0.0 39.119619140206154 0.0 74-75 0.014773090101553081 0.0 0.0 40.872732211531584 0.0 76-77 0.014773090101553081 0.0 0.0 42.651817328358135 0.0 78-79 0.014773090101553081 0.0 0.0 44.45508742333482 0.0 80-81 0.014773090101553081 0.0 0.0 46.230598405459375 0.0 82-83 0.014773090101553081 0.0 0.0 48.0806896650321 0.0 84-85 0.014773090101553081 0.0 0.0 50.03383514184549 0.0 86-87 0.014773090101553081 0.0 0.0 51.670669697532894 0.0 88-89 0.014773090101553081 0.0 0.0 53.07256446547625 0.0 90-91 0.014773090101553081 0.0 0.0 54.80447100423655 0.0 92-93 0.014773090101553081 0.0 0.0 56.31251757282895 0.0 94-95 0.014773090101553081 0.0 0.0 57.685580987509596 0.0 96-97 0.014773090101553081 0.0 0.0 59.002649625192404 0.0 98-99 0.014773090101553081 0.0 0.0 60.16555391939612 0.0 100-101 0.014773090101553081 0.0 0.0 61.27842032777198 0.0 102-103 0.014773090101553081 0.0 0.0 62.3422019529072 0.0 104-105 0.014773090101553081 0.0 0.0 63.35296724662959 0.0 106-107 0.014773090101553081 0.0 0.0 64.29046277896121 0.0 108-109 0.014773090101553081 1.1913782339962162E-4 0.0 65.19233610209635 0.0 110-111 0.014773090101553081 2.3827564679924324E-4 0.0 66.05799152691799 0.0 112-113 0.014773090101553081 2.3827564679924324E-4 0.0 66.879327681435 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGACTA 25 0.0020572501 71.38849 9 TTAGGGC 40 1.7710477E-4 59.497498 4 TTATGCC 30 0.0042353855 59.497498 4 TAACAAC 215 0.0 55.419147 1 TTCACTA 35 0.0077977926 50.99178 8 GTCTTAG 65 3.4900688E-5 45.827377 1 TCTTAGG 65 3.517221E-5 45.767307 2 TCGTAGG 70 5.4473854E-5 42.49821 2 GTCATAG 130 9.0221874E-10 41.24464 1 CCATCCG 30 0.0013085811 39.664997 72-73 GTAGTAG 95 7.3962074E-6 37.626686 1 AGGTATA 40 1.19833145E-4 37.181507 10-11 GTACTAG 65 0.0019254573 36.661903 1 AGTAGAG 295 0.0 36.30356 2 TATAAGG 100 1.0608885E-5 35.698498 2 AGTAAGA 190 2.910383E-11 34.44592 2 CGCAGAA 330 0.0 34.256134 2 ATCCGAG 70 0.0027696826 34.043194 1 TTAGGGA 70 0.0027875535 33.99857 4 GTAGGGT 105 1.4818752E-5 33.99857 4 >>END_MODULE