FastQCFastQC Report
Thu 26 May 2016
SRR935989_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935989_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419682
Sequences flagged as poor quality0
Sequence length125
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT73711.7563297925572219No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT37840.9016350474883364TruSeq Adapter, Index 1 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT21420.5103864354439791RNA PCR Primer, Index 1 (95% over 23bp)
ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA10160.2420880571480311No Hit
GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT9560.22779151834007652No Hit
GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT9360.22302600540409168No Hit
ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC8220.19586258166897794No Hit
GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA7710.18371052368221652No Hit
TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC7240.1725115682826521No Hit
TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC6430.1532112408919134No Hit
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTG4710.11222782964244356TruSeq Adapter, Index 1 (95% over 24bp)
GGGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGA4640.11055990011484887No Hit
TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCT4330.10317335506407233No Hit
GGTATCAACGCAGAGTGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4240.10102887424287912No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTACA552.0008883E-1186.788521
ATACTTG150.004237144759.49648788-89
GTGTATA901.6279955E-952.8857656
GAAGGGT453.1695302E-452.8857656
GTCTAAG350.00770688851.1432341
TAGACAT350.007757988851.0578232
CTAATTT350.007757988851.0578233
GTTTAGT505.255518E-447.7336851
AGTTAGT802.2913264E-644.6223687
TATAAGG705.4097385E-542.5481872
GTCTGAG600.00128965439.7780721
GTGTAAG758.0456586E-539.778071
TGTGTAT1202.0672815E-839.673785
GTATGGT602.0760126E-839.66432614-15
AGTTAAC602.0760126E-839.66432612-13
TATAAGA1202.071647E-839.6643269
GAGATGT1401.8353603E-938.3574261
ATGTGTA1252.9380317E-838.1231774
GTTAGAG957.403136E-637.6215553
TAACACG401.19761105E-437.185307112-113
Produced by FastQC (version 0.11.5)