##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935989_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 419682 Sequences flagged as poor quality 0 Sequence length 125 %GC 50 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.323895234963615 33.0 31.0 34.0 30.0 34.0 2 32.42893190558566 34.0 31.0 34.0 31.0 34.0 3 32.4195414623453 34.0 31.0 34.0 30.0 34.0 4 35.91545265224622 37.0 35.0 37.0 35.0 37.0 5 35.90425607960313 37.0 35.0 37.0 35.0 37.0 6 35.990535691309134 37.0 35.0 37.0 35.0 37.0 7 35.969038462454904 37.0 35.0 37.0 35.0 37.0 8 36.00080298892971 37.0 35.0 37.0 35.0 37.0 9 37.67356236388504 39.0 37.0 39.0 35.0 39.0 10-11 37.60393226299912 39.0 37.0 39.0 35.0 39.0 12-13 37.563029865469574 39.0 37.0 39.0 35.0 39.0 14-15 38.669075633455805 40.0 38.0 41.0 34.0 41.0 16-17 38.646314352295306 40.0 38.0 41.0 34.5 41.0 18-19 38.729348649691914 40.0 38.0 41.0 34.0 41.0 20-21 38.82414780714922 40.0 38.0 41.0 35.0 41.0 22-23 38.664697318445874 40.0 38.0 41.0 34.0 41.0 24-25 38.33168089172278 40.0 38.0 41.0 33.5 41.0 26-27 38.14937023746551 40.0 37.0 41.0 33.0 41.0 28-29 38.02235263842624 40.0 37.0 41.0 33.0 41.0 30-31 37.80240396300056 40.0 36.5 41.0 33.0 41.0 32-33 37.34401761333581 40.0 35.0 41.0 33.0 41.0 34-35 37.04497571971159 39.0 35.0 41.0 32.5 41.0 36-37 36.80940211874705 39.0 35.0 41.0 32.0 41.0 38-39 36.26843657817109 39.0 35.0 41.0 30.5 41.0 40-41 35.92898551760619 39.0 35.0 41.0 30.0 41.0 42-43 35.49431831720207 39.0 35.0 41.0 28.5 41.0 44-45 34.89584137513641 38.0 35.0 41.0 26.5 41.0 46-47 34.14429734894515 37.5 35.0 40.5 21.5 41.0 48-49 33.17370652065135 37.0 34.0 40.0 5.0 41.0 50-51 32.2182354735252 36.0 33.0 39.5 2.0 41.0 52-53 31.63624363208334 35.5 33.0 39.0 2.0 41.0 54-55 31.13786271510334 35.0 33.0 38.5 2.0 41.0 56-57 30.777065730719926 35.0 32.0 37.0 2.0 40.0 58-59 30.484053402337963 35.0 32.0 37.0 2.0 39.0 60-61 30.088407413231923 35.0 32.0 36.0 2.0 39.0 62-63 29.766380259339215 35.0 32.0 35.5 2.0 37.5 64-65 29.474206661233982 35.0 31.0 35.0 2.0 37.0 66-67 29.016778179669366 35.0 31.0 35.0 2.0 36.5 68-69 28.534019805471765 35.0 30.0 35.0 2.0 36.0 70-71 28.03491095639079 35.0 29.5 35.0 2.0 35.0 72-73 27.735799962829 35.0 29.0 35.0 2.0 35.0 74-75 27.62909178854466 35.0 29.0 35.0 2.0 35.0 76-77 27.51782778389352 35.0 29.0 35.0 2.0 35.0 78-79 27.55371567043619 35.0 29.0 35.0 2.0 35.0 80-81 27.523893090482794 35.0 29.0 35.0 2.0 35.0 82-83 27.450339781072337 35.0 29.0 35.0 2.0 35.0 84-85 27.392134997450448 35.0 29.0 35.0 2.0 35.0 86-87 27.375419960827486 35.0 29.0 35.0 2.0 35.0 88-89 27.315955890412262 35.0 28.0 35.0 2.0 35.0 90-91 27.216244680496185 35.0 27.0 35.0 2.0 35.0 92-93 27.136794048827447 35.0 27.0 35.0 2.0 35.0 94-95 27.106155374783768 34.5 27.0 35.0 2.0 35.0 96-97 27.071358790703435 34.5 27.0 35.0 2.0 35.0 98-99 26.99539413174737 34.0 27.0 35.0 2.0 35.0 100-101 26.895774657955307 34.0 25.0 35.0 2.0 35.0 102-103 26.172219680615324 33.5 24.5 35.0 2.0 35.0 104-105 26.55604243212718 34.0 25.0 35.0 2.0 35.0 106-107 26.395261173936458 34.0 24.0 35.0 2.0 35.0 108-109 26.065443597771647 34.0 21.0 35.0 2.0 35.0 110-111 25.610153640137057 34.0 12.0 35.0 2.0 35.0 112-113 25.042115220571766 34.0 2.0 35.0 2.0 35.0 114-115 24.33846221663069 34.0 2.0 35.0 2.0 35.0 116-117 23.63786867199451 34.0 2.0 35.0 2.0 35.0 118-119 23.050602599110753 33.0 2.0 35.0 2.0 35.0 120-121 22.391503566986433 33.0 2.0 35.0 2.0 35.0 122-123 21.65912762520194 33.0 2.0 35.0 2.0 35.0 124-125 19.567924047254827 28.5 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12334697264270744 1101 2 0.14657890330997247 1101 3 0.11701691741730968 1101 4 0.1290692482467648 1101 5 0.2977776532405656 1101 6 0.15487387853353596 1101 7 0.0926805472391905 1101 8 0.1882137232668626 1101 9 0.17963905803575386 1101 10-11 0.2515546651276921 1101 12-13 0.09342124684467734 1101 14-15 0.10677126556313965 1101 16-17 0.2529272252467578 1101 18-19 0.11751249923322149 1101 20-21 0.10394025220968928 1101 22-23 0.22655083573334167 1101 24-25 0.3170436348437988 1101 26-27 0.35381595834250845 1101 28-29 0.3679699774042362 1101 30-31 0.5011846817248937 1101 32-33 0.5744814302066459 1101 34-35 0.742431796705965 1101 36-37 0.6416643486461311 1101 38-39 0.804356609438166 1101 40-41 0.6888541779565998 1101 42-43 0.40185478798083096 1101 44-45 0.8781390088792378 1101 46-47 0.974636077254253 1101 48-49 0.8013679153624338 1101 50-51 1.1045740224397704 1101 52-53 1.190312570880053 1101 54-55 1.1548055042978298 1101 56-57 1.0751713605447293 1101 58-59 1.0730888609553126 1101 60-61 0.9268236331514821 1101 62-63 1.0090238688720348 1101 64-65 1.0328861751897982 1101 66-67 0.87216666321698 1101 68-69 0.8834933924741328 1101 70-71 0.8714663445506083 1101 72-73 0.9851085707975251 1101 74-75 1.0567835901952307 1101 76-77 1.204457528441548 1101 78-79 0.9685790212494965 1101 80-81 0.9066508831346347 1101 82-83 1.0036123164279758 1101 84-85 0.8978038747820598 1101 86-87 0.8064250760005116 1101 88-89 0.482843031234367 1101 90-91 0.7268454707765315 1101 92-93 0.7094544083930145 1101 94-95 0.749930200592825 1101 96-97 0.7647374960724029 1101 98-99 0.6407201911376852 1101 100-101 0.5147572032980108 1101 102-103 0.527948584913247 1101 104-105 0.6845731568445714 1101 106-107 0.6705282235802912 1101 108-109 0.9284563596745237 1101 110-111 0.8324323215014608 1101 112-113 0.8515136299383173 1101 114-115 0.7899110064155792 1101 116-117 0.6848191610232028 1101 118-119 0.16145801366636903 1101 120-121 -0.08098854042293624 1101 122-123 -0.30938159141846455 1101 124-125 -0.48839308211409005 1102 1 0.19444697979955805 1102 2 0.21941281895028197 1102 3 0.07550396027379946 1102 4 0.25529648459833254 1102 5 0.256115052963402 1102 6 0.22338440885578592 1102 7 0.3286449972356138 1102 8 0.1129139490328086 1102 9 0.24090165571043087 1102 10-11 0.22200990556518008 1102 12-13 0.1835812348732233 1102 14-15 0.3849724900343503 1102 16-17 0.3281017546841696 1102 18-19 0.11172521369460497 1102 20-21 0.31504930301678513 1102 22-23 0.4090438903400724 1102 24-25 0.2588357089626925 1102 26-27 0.16875945179400276 1102 28-29 0.1455173264435885 1102 30-31 0.14639545082553695 1102 32-33 -0.0478615908930351 1102 34-35 -0.2400225458727121 1102 36-37 -0.2897860836923982 1102 38-39 -0.07576460150009723 1102 40-41 -0.13931158759815077 1102 42-43 -0.22637636511710468 1102 44-45 -0.4174029269824189 1102 46-47 -0.5031728219578397 1102 48-49 -0.9968090408962311 1102 50-51 -1.1799902883234736 1102 52-53 -1.0892273090150653 1102 54-55 -1.036456312630058 1102 56-57 -0.9014403935981115 1102 58-59 -0.9487102398846439 1102 60-61 -1.0942874345848104 1102 62-63 -1.0070887928889363 1102 64-65 -1.0887736924736195 1102 66-67 -1.3344563466919972 1102 68-69 -1.3001740354206497 1102 70-71 -1.284783858768865 1102 72-73 -1.5854855775053274 1102 74-75 -1.6107858149407797 1102 76-77 -1.5898270797862217 1102 78-79 -1.683881307054527 1102 80-81 -1.561343378376499 1102 82-83 -1.6298449604573655 1102 84-85 -1.5995692375999333 1102 86-87 -1.4513954042888848 1102 88-89 -1.4209201682659511 1102 90-91 -1.442191087691647 1102 92-93 -1.410193409745034 1102 94-95 -1.595737305376634 1102 96-97 -1.5367218497919133 1102 98-99 -1.530266607735438 1102 100-101 -1.497479375990082 1102 102-103 -1.4081863281310767 1102 104-105 -1.4576219778017183 1102 106-107 -1.3116870152956928 1102 108-109 -1.4325381816042082 1102 110-111 -1.378065144324605 1102 112-113 -1.815608472352526 1102 114-115 -1.887347791624748 1102 116-117 -1.7821861545611526 1102 118-119 -1.670237377975738 1102 120-121 -1.4274953491855165 1102 122-123 -1.5876917951283112 1102 124-125 -1.5349174576793203 1103 1 0.0950419443376802 1103 2 0.07614933288040504 1103 3 0.08671388711427852 1103 4 0.1125857317632466 1103 5 0.06126416672708501 1103 6 0.14138736504701654 1103 7 0.14729260185124105 1103 8 0.2525660376191752 1103 9 0.22825710665379972 1103 10-11 0.327811741384771 1103 12-13 0.07997635839979012 1103 14-15 0.07363773242961003 1103 16-17 0.3194024167219496 1103 18-19 0.2878421695628859 1103 20-21 0.28413505740449807 1103 22-23 0.2670520012345108 1103 24-25 0.3061378739380416 1103 26-27 0.08146331098986792 1103 28-29 0.1681281525624101 1103 30-31 0.27561858115879545 1103 32-33 0.25492598565119806 1103 34-35 0.48048540975958076 1103 36-37 0.7686623506441279 1103 38-39 0.693217748299304 1103 40-41 0.4240772881797099 1103 42-43 0.6243406354666803 1103 44-45 0.577065082805305 1103 46-47 0.595556823174995 1103 48-49 0.4515926905872121 1103 50-51 0.7125077553735011 1103 52-53 0.794291924859408 1103 54-55 0.6924761669685005 1103 56-57 0.756614916988287 1103 58-59 0.7339696468360977 1103 60-61 0.7832588645867133 1103 62-63 0.8118460466942139 1103 64-65 0.8488202411238639 1103 66-67 0.8778692939196091 1103 68-69 0.7154530994338408 1103 70-71 0.7211999442842121 1103 72-73 0.5038398395287942 1103 74-75 0.4336983421099845 1103 76-77 0.4768518008358207 1103 78-79 0.42212547479594775 1103 80-81 0.5526299041136546 1103 82-83 0.6710282338438915 1103 84-85 0.5783316803098622 1103 86-87 0.592472362047797 1103 88-89 0.5205553189466556 1103 90-91 0.46847883740989715 1103 92-93 0.7679792169178228 1103 94-95 0.6504463511089753 1103 96-97 0.7467138530487638 1103 98-99 0.8705736709911633 1103 100-101 0.9542760178168237 1103 102-103 0.8501264070910715 1103 104-105 0.7837656310370456 1103 106-107 0.9295608576129268 1103 108-109 0.7768995831177499 1103 110-111 0.9866114756806148 1103 112-113 0.9770963805210648 1103 114-115 1.0625134040179738 1103 116-117 1.0587369099409543 1103 118-119 1.4057137579221148 1103 120-121 1.4402818558474593 1103 122-123 1.318199161162287 1103 124-125 1.0970631283421213 1104 1 0.1386930300940321 1104 2 0.07796506101192335 1104 3 0.09205065560894354 1104 4 0.0442996318981983 1104 5 0.28921779204912923 1104 6 0.05236761286937508 1104 7 0.10035357596484573 1104 8 -0.0359813798756079 1104 9 -0.0685776375493603 1104 10-11 -0.06977751146764177 1104 12-13 0.037512243567256576 1104 14-15 0.19557982542433194 1104 16-17 0.22638008817330757 1104 18-19 0.35376659601363514 1104 20-21 0.2186983011782644 1104 22-23 0.28964125408691643 1104 24-25 0.11719786657697284 1104 26-27 0.27177431577455025 1104 28-29 0.3047831642174188 1104 30-31 0.27941872837999426 1104 32-33 0.23953830315825542 1104 34-35 0.2876409734940921 1104 36-37 0.3480360471230952 1104 38-39 0.4239440395519125 1104 40-41 0.34817555833060965 1104 42-43 0.33527487666408007 1104 44-45 0.5067393559006348 1104 46-47 0.37456860087098676 1104 48-49 0.835530244261605 1104 50-51 0.9204116935406006 1104 52-53 1.0925682099777916 1104 54-55 1.0500243205692819 1104 56-57 0.9298175915067475 1104 58-59 0.9516699290100625 1104 60-61 0.9584796963864939 1104 62-63 0.7773196082204095 1104 64-65 0.6984828592075338 1104 66-67 0.7971522039919954 1104 68-69 0.551789131822499 1104 70-71 0.543473284978603 1104 72-73 0.6454420969205223 1104 74-75 0.5421604939931868 1104 76-77 0.5702084441924669 1104 78-79 0.6970058575710674 1104 80-81 0.6822919087756638 1104 82-83 0.710446710104474 1104 84-85 0.9032212644099751 1104 86-87 0.7383992421852028 1104 88-89 0.78349851610038 1104 90-91 0.7993922222706473 1104 92-93 0.6698072134826631 1104 94-95 0.5719467805041418 1104 96-97 0.37543732256170514 1104 98-99 0.40471672092369104 1104 100-101 0.6383546584744124 1104 102-103 0.45931546364854725 1104 104-105 0.4572934190385176 1104 106-107 0.5996105447678772 1104 108-109 0.6269333213093837 1104 110-111 0.44360448267362074 1104 112-113 0.4128496623269804 1104 114-115 0.09769006945779779 1104 116-117 0.07045633087089698 1104 118-119 -0.08434881108782477 1104 120-121 -0.1274797684931137 1104 122-123 0.15630184794918378 1104 124-125 0.12862060595222857 1105 1 -0.0077691518509013235 1105 2 0.06860059023232168 1105 3 0.08394678666505939 1105 4 0.13041558403018172 1105 5 0.14746108239419442 1105 6 0.06996375554393808 1105 7 0.058027562255077214 1105 8 0.10447406336825793 1105 9 0.1589406615095399 1105 10-11 0.27816602286488745 1105 12-13 0.288209350573176 1105 14-15 0.27543924217813043 1105 16-17 0.24715678156240983 1105 18-19 0.06477798209473207 1105 20-21 0.11241852889988735 1105 22-23 0.1751414283636663 1105 24-25 0.2616558658798738 1105 26-27 0.2939858877508499 1105 28-29 0.223105460023163 1105 30-31 0.3564839078850497 1105 32-33 0.3018393372003132 1105 34-35 0.28965967919874913 1105 36-37 0.27629792698831324 1105 38-39 0.06205255587583025 1105 40-41 0.11070605676211898 1105 42-43 0.31106202665827 1105 44-45 0.34257936984277393 1105 46-47 0.19226640498391845 1105 48-49 0.31995280729169195 1105 50-51 0.4052496161617256 1105 52-53 0.3526036462505928 1105 54-55 0.3193980861089578 1105 56-57 0.334250030219426 1105 58-59 0.32018392924243244 1105 60-61 0.337043655954286 1105 62-63 0.47472869384838745 1105 64-65 0.4176562064830094 1105 66-67 0.35574985706507434 1105 68-69 0.49710108720765334 1105 70-71 0.21104619075297393 1105 72-73 0.17451436368013873 1105 74-75 0.30178637990000823 1105 76-77 0.34289056339775925 1105 78-79 0.2790640660060646 1105 80-81 0.2115413568992821 1105 82-83 0.17268965189603946 1105 84-85 0.17023067535455638 1105 86-87 0.2334439732180833 1105 88-89 0.29780373278447314 1105 90-91 0.23846216534024478 1105 92-93 0.3165366197798605 1105 94-95 0.46404913904951073 1105 96-97 0.33867073722418795 1105 98-99 0.24659251969213258 1105 100-101 0.2683534966294019 1105 102-103 0.2776029041039898 1105 104-105 0.21659108373004798 1105 106-107 0.06059869394877637 1105 108-109 0.24576101969441666 1105 110-111 0.3164521647928247 1105 112-113 0.4074441784185332 1105 114-115 0.5222630914166686 1105 116-117 0.3586026071576427 1105 118-119 0.4452346674761358 1105 120-121 0.44853684589052634 1105 122-123 0.3347612414660901 1105 124-125 0.600698565752392 1106 1 0.04157927797179184 1106 2 -0.02520415234405249 1106 3 -0.09394255321957701 1106 4 0.04670483846299334 1106 5 -0.04422872747549178 1106 6 0.12491996148283846 1106 7 0.13136659237684967 1106 8 0.1337413139557455 1106 9 0.1971269464913803 1106 10-11 0.029458481741194475 1106 12-13 0.15482973083380358 1106 14-15 0.06207671280407112 1106 16-17 0.13157250469848947 1106 18-19 0.25583224400457993 1106 20-21 0.19490090446066688 1106 22-23 0.3033084866845357 1106 24-25 0.11049158474335741 1106 26-27 0.1670204957406014 1106 28-29 0.15935305669054145 1106 30-31 0.19096371989103034 1106 32-33 0.19928646388265037 1106 34-35 0.15647232606907835 1106 36-37 0.046352402527730874 1106 38-39 0.1975150638869465 1106 40-41 0.1875219080759436 1106 42-43 -0.04433746063076427 1106 44-45 -0.14703823323348786 1106 46-47 -0.10667078792358353 1106 48-49 -0.07625526790591408 1106 50-51 -0.1385017190230613 1106 52-53 -0.0838041725915275 1106 54-55 0.016252390744721623 1106 56-57 -0.19082474012879302 1106 58-59 -0.12593335338948108 1106 60-61 -0.1103233596406703 1106 62-63 -0.17150608504501008 1106 64-65 -0.1348646223997072 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-1.5750848603441128 2216 52-53 -1.2806695719770929 2216 54-55 -1.375414275921944 2216 56-57 -1.0994508922025226 2216 58-59 -0.8998964870300377 2216 60-61 -1.0839936745408245 2216 62-63 -0.7960356549374836 2216 64-65 -0.951603293674669 2216 66-67 -1.1519862671859507 2216 68-69 -1.010851845621108 2216 70-71 -0.9630116774274136 2216 72-73 -0.8401547075907523 2216 74-75 -0.8552470874604481 2216 76-77 -1.0591070319980105 2216 78-79 -1.0422955208750473 2216 80-81 -0.9561009483671974 2216 82-83 -0.9233565480408892 2216 84-85 -1.08588431203113 2216 86-87 -1.2630575247320905 2216 88-89 -1.0454880127216732 2216 90-91 -1.0293358479047896 2216 92-93 -1.4137140897754819 2216 94-95 -1.3477117407991166 2216 96-97 -1.1612011486162395 2216 98-99 -1.2032015121590156 2216 100-101 -1.1368568076910002 2216 102-103 -1.2449451879805231 2216 104-105 -1.0890646636682497 2216 106-107 -0.8649571244050556 2216 108-109 -0.6322693729262063 2216 110-111 -0.9781217078025684 2216 112-113 -0.7297020294023433 2216 114-115 -0.7083719606173915 2216 116-117 -0.3467742455702023 2216 118-119 -0.11172193138857622 2216 120-121 -0.5832206832800786 2216 122-123 -0.6288641921510667 2216 124-125 -1.3149727524437616 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 6.0 4 26.0 5 86.0 6 286.0 7 668.0 8 1281.0 9 1797.0 10 2759.0 11 5124.0 12 7643.0 13 8307.0 14 11186.0 15 14256.0 16 6374.0 17 2336.0 18 2088.0 19 2503.0 20 3682.0 21 6139.0 22 6260.0 23 1981.0 24 2408.0 25 3137.0 26 4166.0 27 5011.0 28 6128.0 29 7774.0 30 9877.0 31 13733.0 32 18358.0 33 24594.0 34 33214.0 35 50677.0 36 112592.0 37 40707.0 38 2512.0 39 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.06617884043785 20.361452974108605 12.078115860718848 15.4942523247347 2 19.492993454521194 21.487613151956612 36.96539498044935 22.053998413072847 3 28.966455554443606 22.737215320170986 28.950729361754853 19.34559976363056 4 15.650589276386247 23.068482956185317 27.701834019888228 33.57909374754021 5 14.926867169233923 34.13064047533361 28.214472125630692 22.728020229801775 6 30.860070292488235 36.31881813307917 9.486388276642641 23.334723297789957 7 26.497443302309843 40.869277214652996 12.359834350770345 20.27344513226681 8 35.25216711700764 27.592796450645967 12.644097197401843 24.510939234944555 9 32.97401365796007 13.973913582188418 18.094176066640934 34.95789669321057 10-11 29.302662492077335 25.042532202953666 18.800663359400687 26.854141945568312 12-13 33.20633241358934 25.86112818753247 20.230793791489745 20.701745607388453 14-15 34.64099008296758 19.335473048641592 24.794963805929253 21.228573062461578 16-17 30.017489432475063 21.194857058439485 26.371514622976445 22.416138886109003 18-19 28.63668205927345 21.040216163666773 26.96946735861914 23.353634418440627 20-21 33.55409572009283 17.904270376141938 27.19189767490624 21.349736228858994 22-23 29.632793400717688 20.993871550364325 27.249798657078454 22.123536391839536 24-25 25.085898370671128 22.13699896588369 29.158982277057394 23.61812038638779 26-27 22.879585019133533 21.49222506564494 31.692686367297146 23.93550354792438 28-29 23.692462388189153 18.81329196868105 34.11130332013286 23.382942322996936 30-31 25.70315143370457 20.35207609571056 30.719211212298838 23.225561258286035 32-33 23.125247210983556 18.895139653356587 32.58443297544331 25.395180160216547 34-35 21.237984950510146 20.419269828107947 33.67776078078164 24.664984440600264 36-37 23.751078197301766 20.666123398191967 30.492849347839556 25.089949056666715 38-39 23.6465943261803 17.21303272477733 32.45493016140792 26.685442787634443 40-41 22.089462974347242 17.51885951744416 33.62367220895821 26.768005299250387 42-43 23.296329125385412 17.60714064458328 34.484562120843876 24.611968109187433 44-45 22.901982929932664 18.185316501541642 33.61402204526284 25.29867852326285 46-47 22.09065435258124 17.483118170424277 32.037471228215644 28.388756248778833 48-49 21.134454181975876 18.74014134511368 31.891765670197913 28.23363880271253 50-51 21.151490890722023 19.13770426179822 30.738868953159777 28.971935894319984 52-53 20.95431779299565 19.155694073131563 30.133410534642895 29.756577599229892 54-55 19.265777421952812 19.791413498791943 28.055527756730097 32.887281322525155 56-57 19.947841460915647 20.518154221529635 26.94849910170081 32.585505215853914 58-59 19.86480239800611 20.59857225232438 26.865460038791277 32.67116531087824 60-61 20.310735270990893 22.243508180002955 23.978750577818445 33.46700597118771 62-63 18.692367077930435 23.292397577213222 24.295776325884834 33.719459018971506 64-65 18.190796841418024 22.941774962948138 24.608632250132242 34.258795945501596 66-67 19.179402499988086 23.806596423005992 24.244427923999602 32.76957315300633 68-69 19.167607855471523 23.76775749257771 24.579800896869536 32.484833755081226 70-71 18.999980937948255 23.1421647819063 25.547438298521264 32.31041598162418 72-73 19.598052811414355 21.89645970043986 26.12358881248183 32.381898675663955 74-75 19.900067193732397 21.646031995653853 26.238675949885863 32.215224860727886 76-77 20.16657850467735 21.34425588898261 26.541405159144304 31.947760447195733 78-79 19.845859484085572 21.880256956457508 27.290305517034323 30.983578042422593 80-81 19.859679471599925 22.36157376299198 27.080027258733995 30.6987195066741 82-83 19.212165401422983 22.092203144285435 27.13363927926382 31.561992175027758 84-85 19.55921388098608 21.115868681525534 27.33903288680477 31.985884550683615 86-87 19.919605796769936 21.23441081580816 26.999370952292452 31.846612435129458 88-89 19.867780843591103 21.405969281503616 26.84806591657493 31.878183958330354 90-91 20.135721808416847 20.748209358514302 26.672099351413692 32.44396948165516 92-93 20.095929775401373 20.57629347934865 26.56475617253063 32.76302057271934 94-95 19.79463022002373 20.571885379882865 26.727141025824313 32.906343374269085 96-97 19.963805929251194 20.644202038686434 26.492677789373857 32.89931424268851 98-99 20.29882148865093 21.31328005489871 26.267149889678375 32.12074856677199 100-101 20.65837943967099 22.151772055985248 25.865059735704655 31.32478876863911 102-103 20.416529658169758 23.247601755614966 25.525278663368933 30.810589922846344 104-105 20.074246691542644 25.053850296176627 25.157381064710897 29.71452194756983 106-107 19.794749357847135 26.867127968318872 24.62602637234859 28.712096301485413 108-109 19.493449802469488 27.928645974809495 24.28195633837048 28.295947884350532 110-111 19.087042508577966 29.24537743042318 24.32615325962638 27.341426801372474 112-113 19.29367865869713 30.204333524351206 24.078259346671228 26.423728470280437 114-115 18.93232684980664 30.767681966455314 24.03789562975512 26.262095553982924 116-117 18.415013951670744 31.220526946621458 24.373485391850128 25.990973709857673 118-119 18.412982457812493 31.672679270100122 24.710846990759492 25.20349128132789 120-121 18.7864339658383 32.01340067027179 24.34685433323683 24.85331103065308 122-123 18.233026943628662 32.108489357773024 24.567966688310527 25.090517010287783 124-125 17.410480729239286 33.48873404431542 25.12200266707662 23.97878255936867 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.5 2 1.0 3 2.0 4 2.5 5 1.5 6 2.0 7 3.0 8 9.5 9 15.5 10 18.5 11 26.0 12 30.0 13 26.5 14 19.5 15 22.5 16 30.5 17 46.5 18 53.5 19 68.5 20 104.5 21 154.5 22 238.0 23 354.0 24 506.5 25 729.5 26 1012.5 27 1366.5 28 1807.0 29 2259.5 30 2861.5 31 3448.0 32 3789.0 33 4211.5 34 4518.5 35 4654.5 36 4685.0 37 4426.5 38 4108.5 39 3794.0 40 3535.0 41 3440.0 42 3386.5 43 3262.0 44 3313.5 45 3683.0 46 4163.5 47 4787.5 48 5890.0 49 8312.5 50 13420.0 51 22347.0 52 33180.0 53 41120.0 54 42272.0 55 38595.5 56 33385.0 57 26049.5 58 18567.5 59 13177.5 60 9650.0 61 7318.0 62 5803.5 63 4633.5 64 3548.5 65 3064.5 66 2760.0 67 2251.5 68 1708.0 69 1216.5 70 859.5 71 594.0 72 390.5 73 266.5 74 147.5 75 80.5 76 42.5 77 19.0 78 11.0 79 6.0 80 5.0 81 3.5 82 3.0 83 1.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19467120343498173 2 7.148269403977297E-4 3 0.0 4 0.10460300894486778 5 0.024542391620322054 6 0.0016679295275947028 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 0.0 108-109 0.0 110-111 4.7655129359848653E-4 112-113 1.1913782339962163E-4 114-115 7.148269403977297E-4 116-117 0.0045272372891856216 118-119 0.0019062051743939461 120-121 3.5741347019886487E-4 122-123 0.0022636186445928108 124-125 0.028950491086108053 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 419682.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.59357429516096 #Duplication Level Percentage of deduplicated Percentage of total 1 82.05493889750285 47.25837219678224 2 8.903427935120675 10.255604765259683 3 3.1077342347317 5.369565676129061 4 1.6134718488267943 3.717024431942268 5 0.9988351281273764 2.8763242580210338 6 0.6629023343691512 2.2907348906955214 7 0.479709023682132 1.9339710106847197 8 0.3791646846889331 1.7469959550186682 9 0.29153084912865856 1.5111273256760485 >10 1.4338511648763623 14.171606473707666 >50 0.050449642619363716 1.9591198300789248 >100 0.01984903971811681 2.345180277905123 >500 0.0024811299647646014 1.1555615627496352 >1k 0.0012405649823823007 1.6533199440659456 >5k 4.1352166079410024E-4 1.755491401283504 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT 7371 1.7563297925572219 No Hit ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT 3784 0.9016350474883364 TruSeq Adapter, Index 1 (95% over 21bp) ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT 2142 0.5103864354439791 RNA PCR Primer, Index 1 (95% over 23bp) ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA 1016 0.2420880571480311 No Hit GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT 956 0.22779151834007652 No Hit GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT 936 0.22302600540409168 No Hit ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC 822 0.19586258166897794 No Hit GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA 771 0.18371052368221652 No Hit TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC 724 0.1725115682826521 No Hit TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC 643 0.1532112408919134 No Hit CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTG 471 0.11222782964244356 TruSeq Adapter, Index 1 (95% over 24bp) GGGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGA 464 0.11055990011484887 No Hit TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCT 433 0.10317335506407233 No Hit GGTATCAACGCAGAGTGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 424 0.10102887424287912 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 2.3827564679924324E-4 0.0 0.0 0.0 0.0 7 2.3827564679924324E-4 0.0 0.0 0.0 0.0 8 4.765512935984865E-4 0.0 0.0 0.0 0.0 9 4.765512935984865E-4 0.0 0.0 0.0 0.0 10-11 9.53102587196973E-4 0.0 0.0 0.0 0.0 12-13 0.001906205174393946 0.0 0.0 0.0 0.0 14-15 0.0022636186445928108 0.0 0.0 0.0 0.0 16-17 0.0023827564679924323 0.0 0.0 0.0010722404105965946 0.0 18-19 0.002501894291392054 0.0 0.0 0.0016679295275947026 0.0 20-21 0.0026210321147916754 0.0 0.0 0.005599477699782217 0.0 22-23 0.002740169938191297 0.0 0.0 0.02132567038853227 0.0 24-25 0.002859307761590919 0.0 0.0 0.04586806200885432 0.0 26-27 0.002859307761590919 0.0 0.0 0.11866127210602315 0.0 28-29 0.003216721231789784 0.0 0.0 0.40089877573972676 0.0 30-31 0.0036932725253882703 0.0 0.0 0.9980175466186303 0.0 32-33 0.003812410348787892 0.0 0.0 1.8483041922217298 0.0 34-35 0.003812410348787892 0.0 0.0 3.0712539494188458 0.0 36-37 0.0044080994657860004 0.0 0.0 4.730962967198975 0.0 38-39 0.0045272372891856216 0.0 0.0 6.856620012295023 0.0 40-41 0.004884650759384487 0.0 0.0 9.25093284915722 0.0 42-43 0.005837753346581459 0.0 0.0 11.14296062256661 0.0 44-45 0.005956891169981081 0.0 0.0 12.83388375007744 0.0 46-47 0.005956891169981081 0.0 0.0 14.815503166683346 0.0 48-49 0.006433442463579567 0.0 0.0 16.987147411611648 0.0 50-51 0.00667171811037881 0.0 0.0 19.051329339833494 0.0 52-53 0.0069099937571780535 0.0 0.0 20.892723538298043 0.0 54-55 0.007148269403977297 0.0 0.0 22.680743991879567 0.0 56-57 0.007148269403977297 0.0 0.0 24.53750696956267 0.0 58-59 0.007148269403977297 0.0 0.0 26.306822784870448 0.0 60-61 0.007148269403977297 0.0 0.0 28.15191025586039 0.0 62-63 0.007505682874176162 0.0 0.0 29.943504844143902 0.0 64-65 0.007624820697575784 0.0 0.0 31.6440781353501 0.0 66-67 0.007624820697575784 0.0 0.0 33.475345618825685 0.0 68-69 0.007624820697575784 0.0 0.0 35.3903669921512 0.0 70-71 0.007624820697575784 0.0 0.0 37.42119032982115 0.0 72-73 0.007624820697575784 0.0 0.0 39.43914678256394 0.0 74-75 0.007624820697575784 0.0 0.0 41.21072621651631 0.0 76-77 0.007863096344375027 0.0 0.0 43.006728904265614 0.0 78-79 0.007863096344375027 0.0 0.0 44.82655915669483 0.0 80-81 0.007863096344375027 0.0 0.0 46.62637425479292 0.0 82-83 0.007863096344375027 0.0 0.0 48.50553514327515 0.0 84-85 0.007863096344375027 0.0 0.0 50.49049041893625 0.0 86-87 0.007863096344375027 0.0 0.0 52.14876978283557 0.0 88-89 0.007863096344375027 0.0 0.0 53.57449211545885 0.0 90-91 0.007863096344375027 0.0 0.0 55.332608975367066 2.3827564679924324E-4 92-93 0.007863096344375027 0.0 0.0 56.86841465681158 2.3827564679924324E-4 94-95 0.007863096344375027 0.0 0.0 58.257561677651175 2.3827564679924324E-4 96-97 0.007863096344375027 0.0 0.0 59.59083305931634 2.3827564679924324E-4 98-99 0.007863096344375027 0.0 0.0 60.77506302390857 2.3827564679924324E-4 100-101 0.007863096344375027 0.0 0.0 61.899247525507405 2.3827564679924324E-4 102-103 0.007863096344375027 0.0 0.0 62.97839792986118 2.3827564679924324E-4 104-105 0.007863096344375027 0.0 0.0 64.00572338103612 2.3827564679924324E-4 106-107 0.007863096344375027 0.0 0.0 64.9595407951735 2.3827564679924324E-4 108-109 0.007863096344375027 0.0 0.0 65.87976134311216 2.3827564679924324E-4 110-111 0.007863096344375027 0.0 0.0 66.76578933573515 2.3827564679924324E-4 112-113 0.007863096344375027 0.0 0.0 67.60559185287909 2.3827564679924324E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTACA 55 2.0008883E-11 86.78852 1 ATACTTG 15 0.0042371447 59.496487 88-89 GTGTATA 90 1.6279955E-9 52.885765 6 GAAGGGT 45 3.1695302E-4 52.885765 6 GTCTAAG 35 0.007706888 51.143234 1 TAGACAT 35 0.0077579888 51.057823 2 CTAATTT 35 0.0077579888 51.057823 3 GTTTAGT 50 5.255518E-4 47.733685 1 AGTTAGT 80 2.2913264E-6 44.622368 7 TATAAGG 70 5.4097385E-5 42.548187 2 GTCTGAG 60 0.001289654 39.778072 1 GTGTAAG 75 8.0456586E-5 39.77807 1 TGTGTAT 120 2.0672815E-8 39.67378 5 GTATGGT 60 2.0760126E-8 39.664326 14-15 AGTTAAC 60 2.0760126E-8 39.664326 12-13 TATAAGA 120 2.071647E-8 39.664326 9 GAGATGT 140 1.8353603E-9 38.357426 1 ATGTGTA 125 2.9380317E-8 38.123177 4 GTTAGAG 95 7.403136E-6 37.621555 3 TAACACG 40 1.19761105E-4 37.185307 112-113 >>END_MODULE