FastQCFastQC Report
Thu 26 May 2016
SRR935988_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935988_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1596541
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC80780.5059688413889778No Hit
GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT80440.5038392374514654No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT69980.4383225986679954No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG62550.3917844890923565No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT55760.3492550457520352No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT41700.26118965939490435No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA40090.25110535839668385No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC25460.15946975367372337No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC23560.14756902578762462No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG20920.13103327756694003No Hit
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC19040.11925782050069494No Hit
GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG18640.1167524041036215No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG18200.11399644606684076No Hit
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA17150.107419728024523No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTAGT17900.073.575051
AGTAGTA13950.072.943552
GAGTACG7250.071.5109561
GAGTAAG11700.065.195171
GAGTCCG7600.063.5130161
GAGTTAG10000.061.9761541
AGTCCGT2500.061.886842
GTATACG1350.061.7995871
GAGTCAG46250.060.301131
GTGGGGT47650.058.0504237
AGTACGG5250.057.8063932
TCGTAGG8550.057.070642
GATAGCG17100.057.0509769
GGTATCA31750.055.744761
AGGGTAA48800.055.4634556
TGGGGTA51900.055.245268
GAGTCGA4000.055.1230281
GGGGTAA58650.055.074079
GAGTCGT5850.055.0084231
GATTGTG45450.054.4471059
Produced by FastQC (version 0.11.5)