FastQCFastQC Report
Thu 26 May 2016
SRR935988_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935988_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1596541
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT85430.5350943070049564No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT72970.45705058623611916No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG68460.4288020163591164No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC67590.42335273569548165No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT58070.3637238254451342No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT46890.293697437146932No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA33750.21139450850307007No Hit
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC22740.1424329221736241No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG21860.13692100610006258No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC21150.13247389199525725No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC20740.129905840188257No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG20250.12683670510184206No Hit
GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG20240.1267740696919152No Hit
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA17420.10911088409254757No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTAGT16050.071.4510651
AGTAGTA12150.068.6741262
GAGTACG6600.066.968461
GATAGCG15350.065.502429
GAGTAAG13050.063.6191
AGGGTAA44100.062.1949276
TACGCGG1550.061.5218852
TCGTAGG8700.060.2843782
GAGTTAG12600.060.2026631
GAGTCCG6650.060.1777151
GAGTCGG8600.057.6450731
ACGGGAT5400.057.309245
GATTGTG45450.057.0731249
GGTATCA35700.056.71711
GGATAGC18150.056.0529568
CGGGATT13650.055.3558736
TAGTAGG27250.055.334422
GTAGTAG27250.055.2352831
GGGATAG18600.055.0166977
GAGTCAG40500.052.797161
Produced by FastQC (version 0.11.5)